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212 results about "Saccharic acid" patented technology

Saccharic acid, also called glucaric acid, is a chemical compound with the formula C₆H₁₀O₈. It is derived by oxidizing a sugar such as glucose with nitric acid. The salts of saccharic acid are called saccharates or glucarates.

Method for preparing activated carbon for biomass-based super capacitor

The invention discloses a method for preparing activated carbon for a biomass-based super capacitor, in particular relates to a new method for preparing activated carbon with high specific surface area and high specific capacitance by the steps of hydrolysis of biomass with concentrated acid, in-situ polycondensation and carbonization of saccharic acid solution and activation under certain conditions. The method comprises the following specific steps of: firstly, hydrolyzing biomasses with inorganic acid with a certain concentration, and filtering to obtain a saccharic acid solution and filter residues; adjusting the concentration of the saccharic acid solution, performing polycondensation and carbonization to prepare hydrothermal carbon; mixing the hydrothermal carbon with an activating agent in a certain mass ratio for activating to finally obtain the activated carbon for the super capacitor. The method for preparing activated carbon for the super capacitor by using biomasses is a pollution-free process route meeting the requirement of sustainable development.
Owner:JILIN UNIV

Process and catalyst for the preparation of aldonic acids

This invention relates to a process for the preparation of aldonic acids (in particular gluconic acid) by oxidation of aldoses with oxygen or with a gas containing oxygen, in the presence of supported bimetallic catalysts based on gold and platinum.
Owner:UNIV DELGI STUDI DI MILANO

Non-fluorinated urethane based coatings

ActiveUS20160090508A1Imparting durable water repellencyIncreased durable water repellencyWater-repelling agents additionSynthetic fibresSaccharic acidPolymer science
The present invention is an aqueous composition comprising at least one hydrophobic compound having at least one linkage of Formula I:—NHC(O)—X—  (I)wherein X is the residue of a cyclic or acyclic sugar alcohol which is substituted with at least one —R1; —C(O)R1; —(CH2CH2O)n(CH(CH3)CH2O)mR2; —(CH2CH2O)n(CH(CH3)CH2O)mC(O)R1; or mixtures thereof; where the cyclic or acyclic sugar alcohol is selected from a saccharide, reduced sugar, aminosaccharide, aldonic acid, or aldonic acid lactone; wherein each n is independently 0 to 20; each m is independently 0 to 20; m+n is greater than 0; each R1 is independently a linear or branched alkyl group having 5 to 29 carbons optionally comprising at least 1 unsaturated bond; each R2 is independently —H, a linear or branched alkyl group having 6 to 30 carbons optionally comprising at least 1 unsaturated bond, or mixtures thereof.
Owner:THE CHEMOURS CO FC LLC

L-threonate ferrous, as well as pharmaceutical composition and use for improving and treating human anemia thereof

A compound of ferrous L-threonate with structure (I), its compostions and methods useful for iron supplementation for mammals, particularly for human body to improve and treat nutritional iron-deficiency anemia, blood loss anemia and hemolytic anemia.
Owner:JUNENG INDUSTRY CO LTD

Preparation method of biomass-based colloidal carbon

InactiveCN102219204ASaccharic acidCarbonization
The invention discloses a preparation method of biomass-based colloidal carbon and in particular relates to a colloidal carbon ball using xylose or glucose as a precursor. The colloidal carbon ball is prepared by hydrolyzing biomass by using dilute acid or concentrated acid respectively to obtain saccharic acid solution of xylose and glucose and performing in-situ polycondensation and carbonization on the saccharic acid solution. The preparation method particular comprises the following steps of: hydrolyzing the biomass by using dilute acid at a certain concentration; filtering to obtain xylosic acid solution and filter residues; adjusting the concentration of the saccharic acid solution and performing polycondensation and carbonization to prepare the colloidal carbon ball with grain size of 160 to 1,800 nm; performing alkali boiling on the residues to remove lignin; performing hydrolysis reaction on the residues and the concentrated sulfuric acid; filtering to obtain acid solution of the glucose; and performing in-situ polycondensation and carbonization to prepare the colloidal carbon ball with grain size of 180 to 2,000 nm. The method for preparing the colloidal carbon from the biomass is a green production route of sustainable development.
Owner:JILIN UNIV

Genetically engineered bacterium for high-yielding L-valine and method for producing L-valine by fermentation

The invention provides a genetically engineered bacterium for high-yielding L-valine. A construction method of the genetically engineered bacterium comprises the steps that starting from an escherichia coli W3110, an acetolactate synthase gene alsS of a bacillus subtilis is integrated on a genome of the escherichia coli W3110 and subjected to high expression; an escherichia coli ppGpp 3'-pyrophosphoric acid hydrolytic enzyme mutant R290E / K292D gene spoT is integrated on the genome of the escherichia coli W3110 and subjected to high expression; genes of frdA, frdB, frdC and frdD of four subunits of a lactic dehydrogenase gene ldhA, a pyruvate formate lyase I gene pflB and fumaric reductase on the genome of the escherichia coli W3110 are knocked out; a branched chain amino acid transaminasegene ilvE of the escherichia coli is replaced with leucine dehydrogenase gene bcd of the bacillus subtilis; and an acetyl-hydroxyl acid isomerized reductase gene ilvC of the escherichia coli is replaced with an encoding gene of a mutant L67E / R68F / K75E. According to the genetically engineered bacterium for the high-yielding L-valine, an L-valine fermentation method is further modified. Double-phasedissolved oxygen control is adopted, and the L-valine yield and the saccharic acid conversion rate are improved.
Owner:TIANJIN UNIV OF SCI & TECH

Jujube-acetic acid beverage and processing technic thereof

The invention discloses jujube-acetic acid beverage and a processing technic thereof. The processing technic comprises the following steps: pretreatment of raw materials, pulp preparation, ultra-high-pressure enzymolysis, saccharic acid adjustment, vaccination, alcohol fermentation, acetic acid fermentation, clarification, sterilization, mixng and bottling. Based on the traditional process, the invention adopts a new technique and the ultra-high-pressure assisted enzymolysis, thereby not only improving the yield of jujube juice, but also reducing the working time; and due to the liquid-state shaker fermentation, the method shortens the fermentation time, facilitates the reduction of the production cost of the enterprise, and improves the economic efficiency. In addition, with the optimized mixing scheme, the invention solves the problem of single taste. The jujube-acetic acid beverage produced in the invention has the advantages of good taste, moderate and refreshing sweetness and sourness, high clarity and transparency, fruity palate and delicate flavor.
Owner:北京中食德源投资管理有限公司

Method for co-producing plurality of saccharic acids from cellulose fuel ethanol

The invention discloses a method for co-producing a plurality of saccharic acids from cellulose fuel ethanol. The method comprises the following steps: performing dilute acid hydrolysis pretreatment or dilute acid steam explosion pretreatment on plant fiber raw materials to obtain a hemicellulose pre-hydrolysate and cellulose-enriched solid material through solid-liquid separation, wherein an enzyme hydrolysate is obtained by neutralizing and performing cellulose enzyme hydrolysis on the solid material, and fuel ethanol is produced by anaerobically fermenting the enzyme hydrolysate through saccharomyces cerevisiae or zymomonas mobilis; and a plurality of saccharic acids are produced by whole-cell co-catalyzing through gluconobacter oxydans or pseudomonas fragi under an aerobiotic condition after the hemicellulose pre-hydrolysate is concentrated and neutralized. The plurality of saccharic acids can be co-produced from fuel ethanol prepared from the plant fiber raw materials, the inhibiting effect of hemicellulose pre-hydrolysate sugar fermentation can be effectively overcome, the utilization efficiency of various carbohydrate substances in the plant fiber raw material can be obviously improved, the pollution load in industrial wastewater is effectively reduced, thereby improving the economic benefit of bio-refinery of the plant fiber raw materials. The utilization rate of total carbohydrate in the raw materials is over 80%, and the carbohydrate conversion rate is more than 90%.
Owner:NANJING FORESTRY UNIV

Isolated alcohol dehydrogenase enzymes and uses thereof

InactiveUS20090203089A1FungiSugar derivativesBiofuelMannitol dehydrogenase activity
Bacterial polynucleotides and polypeptides are provided in which the polypeptides have a dehydrogenase activity, such as an alcohol dehydrogenase (ADH) activity, an uronate, a 4-deoxy-L-erythro-5-hexoseulose uronate (DEHU) ((4S,5S)-4,5 dihydroxy-2,6-dioxohexanoate) hydrogenase activity, a 2-keto-3-deoxy-D-gluconate dehydrogenase activity, a D-mannuronate hydrogenase activity, and / or a D-mannnonate dehydrogenase activity. Methods, enzymes, recombinant microorganism, and microbial systems are also provided for converting polysaccharides, such as those derived from biomass, into suitable monosaccharides or oligosaccharides, as well as for converting suitable monosaccharides or oligosaccharides into commodity chemicals, such as biofuels. Commodity chemicals produced by the methods described herein are also provided.
Owner:BIO ARCHITECTURE LAB

Bacillus coagulans used to preparing L-lactic acid and application method thereof

The invention pertains to the technical field of lactic acid preparation, and relates to a bacillus coagulans used to preparing L-lactic acid and application methods thereof. Pentose or hexose is taken as raw material, or agricultural and industrial wastes containing the pentose, the hexose or combinations thereof are taken as raw materials. Ferment is performed on the raw materials to obtain theL-lactic acid. By applying the method of the invention, the highest yield can reach 195g / L, optical purity is greater than 99%, the highest conversion rate of saccharic acid can reach 0.98, and throughput of the ferment process is 2.7g / L / h. By using the bacillus coagulans XZL4(DSM No.23183) and the bacillus coagulans XZL9(DSM No.23184) provided in the invention, high concentration of L-lactic acid can be produced by directly fermenting various reducing sugar in by-products produced in xylitol productions. According to the invention, low cost is achieved, and production efficiency is improved at the same time. The bacillus coagulans and the methods are applicable to popularization and application in industrial productions.
Owner:SHANGHAI JIAO TONG UNIV

Preparation method of azithromycin intermediate

The invention discloses a preparation method of azithromycin intermediate, in particular to a preparation method of azithromycin intermediate 9- deoxidation-alpha- heterocyclic nitrogen-9a-erythromycin A, belonging to the field of pharmaceutical chemical synthesis. The preparation method comprises the steps of: in acid aqueous solution, under the action of potassium borohydride or sodium borohydride, carrying out reduction reaction on erythromycin A 6, 9-imine ether, adding organic saccharic acid for hydrolysis reaction, and obtaining the 9-deoxidation-alpha-heterocyclic nitrogen-9a-erythromycin A. By adopting the method, the hydrolysis of boric acid ester is thorough, the products of acid degradation are less, the yield is high, the production cost of azithromycin is remarkably reduced, and the industrialized production can be very smoothly carried out.
Owner:KAIFENG PHARMA GRP +1

Fermentation production method for citric acid

The invention discloses a fermentation production method for citric acid, and in particular relates to a continuous fermentation production method for citric acid. A citric acid continuous fermentation system consists of a culture medium storage tank, a seed culture tank, two or more serially connected fermentation tanks and a fermentation mash storage tank. By the citric acid continuous fermentation system, a fermentation period can be saved, the conversion rate of saccharic acid and the utilization rate of the fermentation tanks are improved, and stages such as growth and development and saccharification effects of aspergillus niger on citric acid production bacteria, fermentation and acid production and the like are controllable sectionally.
Owner:RIZHAO JINHE BOYUAN BIOCHEM

Method for producing polymer grade L-lactic acid by bacillus by utilizing hydrolysate of maize straws

InactiveCN102643874AStrong ability to produce L-lactic acidAvoid pollutionMicroorganism based processesFermentationSaccharic acidHydrolysate
The invention discloses a method for producing polymer grade L-lactic acid. The method comprises the following steps: inoculating bacillus sp. into a fermentation culture medium taking maize straw hydrolysate as an only carbon source for fermentation culturing to obtain the L-lactic acid; the mass ratio of glucose, xylose and arabinose in the maize straw hydrolysate is (27-29): (8-10):1; and the bacillus sp. is bacillus coagulans XZL4DSM No.23183. With adoption of the method, the glucose in the maize straw hydrolysate can be efficiently utilized; the xylose also can be utilized; the yield of the L-lactic acid can reach 80g / liter; the conversion rate of saccharic acid can reach 94-98%; the condition that D-lactic acid exists in a fermentation liquid cannot be detected; and the optical purity can reach 100%; and in addition, the method also can lower the cost of the fermenting the raw materials and is beneficial for industrial production.
Owner:INST OF MICROBIOLOGY - CHINESE ACAD OF SCI

Method for synchronously determining monosaccharide, uronic acid and saccharic acid in wood fiber material reaction system

The invention discloses a method for synchronously determining monosaccharide, uronic acid and saccharic acid in a wood fiber material reaction system. The method comprises the steps as follows: pretreating the wood fiber material reaction system to obtain a solution to be determined; determining standard samples of monosaccharide, uronic acid and saccharic acid by adopting an integrated pulse amperometric detection method and a chromatographic peak integration method and utilizing a high-performance liquid ion exchange chromatograph, thus obtaining a standard equation; determining the solution to be determined by utilizing the high-performance liquid ion exchange chromatograph and calculating the content of each component by utilizing the standard equation; and separating and quantifyingxylose and mannose in the solution to be determined by utilizing a high-performance liquid chromatography, and then amending the result of the high-performance liquid ion exchange chromatography. Themethod for synchronously, correctly and quantitatively determining the monosaccharide, the uronic acid and the saccharic acid is established for the first time, the separation degrees and the detection efficiencies of eight types of materials are improved greatly and the synchronous, correct and quantitative determination on nine types of materials in the wood fiber material reaction system can be realized under the combination of the high-performance liquid ion exchange chromatography; and the method has significance to matter changing, and component analysis and determination on products and intermediate products in bio-refinery process of the wood fiber material.
Owner:NANJING FORESTRY UNIV

Escherichia coli gene engineering bacterium and method for producing L-threonine by using escherichia coli gene engineering bacterium

The invention belongs to the technical field of gene engineering, and particularly relates to an escherichia coli gene engineering bacterium and a method for producing L-threonine by using the escherichia coli gene engineering bacterium. The gene engineering bacterium is characterized in that a promoter P<ppc> of phosphoenolpyruvate carboxylase gene (ppc) of an initial strain is replaced into a promoter P<zwf> of 6-phosphate dehydrogenase gene (zwf), so that the goal of regulating the L-threonine production capacity by glycine betaine is achieved. In the fermentation process, the glycine betaine is added, so that the L-threonine yield through shaking flask fermentation can reach 50 to 55g / L; the 5L fermentation tank yield reaches 120 to 150g / L; the saccharic acid conversion rate reaches 59 to 61 percent.
Owner:TIANJIN UNIV OF SCI & TECH

Method for preparing glycosyl amide modified polysiloxane

ActiveCN101942097AToxicAvoid the hydrosilylation reaction stepPharmaceutical non-active ingredientsSaccharic acidAlcohol
The invention discloses a method for preparing glycosyl amide modified polysiloxane. The method comprises the following steps of: reacting cyclosiloxane, hexamethyldisiloxane and aminosilane serving as raw materials under the action of a catalyst at the temperature of between 30 and 220 DEG C for 4 to 8 hours, wherein the molar ratio of the cyclosiloxane to the aminosilane to the hexamethyldisiloxane is 0.25-2.25:3-5:1; the molar ratio of the catalyst to the sum of the cyclosiloxane, the aminosilane and the hexamethyldisiloxane is 0.1 to 1; inactivating the catalyst after the reaction is finished; performing reduced pressure distillation to obtain the amino-polysiloxane; reacting the amino-polysiloxane and glycosyl lactone or saccharic acid in low-carbon alcohol serving as a solvent at the temperature of between 30 and 200 DEG C for 5 to 10 hours, wherein the molar ratio of primary amine of the amino-polysiloxane to the glycosyl lactone or saccharic acid is 1-5:1; and evaporating off the solvent to obtain the glycosyl amide modified polysiloxane after the reaction is finished. The method has the advantages of low cost, no toxicity and large-scale industrialization.
Owner:CHINA RES INST OF DAILY CHEM IND

L-lysine fermenting method

The invention relates to the field of fermentation, in particular to an L-lysine fermenting method. The L-lysine fermenting method comprises the following steps: inoculating a lysine first stage seed medium with a lysine shake flask seed, culturing the seed till the seed is mature, then, inoculating the seed in a lysine second stage seed medium for culturing till the seed reaches a mature standard of a lysine second stage seed, inoculating a lysine fermenting medium with the second stage seed, and under conditions of a fed-batch carbon source and a fed-batch nitrogen source, performing fermentation culture till the end of fermentation, wherein the nitrogen source in the lysine primary seed medium, the lysine secondary seed medium and the lysine fermenting medium is ammonium chloride, and during fermentation culture, the fed-batch nitrogen source is an ammonium chloride solution. By the L-lysine fermenting method, the final content of lysine, the total acid amount and the conversion ratio of saccharic acid can be significantly increased.
Owner:ANHUI BBCA FERMENTATION TECH ENG RES

Non-fluorinated and partially fluorinated polymers

InactiveUS20160090505A1Imparting durable water repellencyIncreased durable water repellencyStain/soil resistant fibresLiquid surface applicatorsSaccharic acidPolymer science
A polymer composition with (A) a first poly(meth)acrylate polymer or urethane polymer; and (B) a second poly(meth)acrylate polymer or urethane polymer which has a residue X incorporated therein, where X is the residue of a cyclic or acyclic sugar alcohol which is substituted with at least one —R1; —C(O)R1; —(CH2CH2O)n(CH(CH3)CH2O)mR2; —(CH2CH2O)n(CH(CH3)CH2O)mC(O)R1; or mixtures thereof; where the cyclic or acyclic sugar alcohol is selected from a saccharide, reduced sugar, aminosaccharide, aldonic acid, or aldonic acid lactone; wherein each n is independently 0 to 20; each m is independently 0 to 20; m+n is greater than 0; each R1 is independently a linear or branched alkyl group having 5 to 29 carbons optionally comprising at least 1 unsaturated bond; and each R2 is independently —H, a linear or branched alkyl group having 6 to 30 carbons optionally comprising at least 1 unsaturated bond, or mixtures thereof.
Owner:THE CHEMOURS CO FC LLC

Black and red Chinese wolfberry fruit compound fermented beverage and preparation method thereof

The invention discloses a novel black and red Chinese wolfberry fruit compound fermented beverage and a preparation method thereof and belongs to the processing field of microbial fermentation technologies of foods. The preparation method mainly comprises the steps of pretreatment of raw materials, juicing, ultrasonic assisted chitosan immobilized complex enzyme treatment, filtration, pasteurization, primary fermentation of a lactic acid bacterium composite leavening agent, secondary fermentation with active dry yeast and acetic acid bacteria, saccharic acid blending, centrifugation, pasteurization, filling and finished beverage. The preparation method is simple in process and low in processing cost, the prepared beverage has the flavor of Chinese wolfberry fruits and also has the smell produced through Chinese wolfberry fruit, a pleasant mellow aroma and soft sour, so that the beverage has more delicate taste, has no bad odor, homogeneous color and luster and good nutritional health-care functions; the total acid of the beverage is 2.83%, pH is 3.70, the aqueous protein of plants is 2.83g / L, the total phenol is 38.17mg / L, the carotenoid is 35.67ug / L, the alcoholic strength is 1% or below, and bacteria and escherichia coli are not detected out.
Owner:NINGXIA UNIVERSITY

Method for fermenting L-lysine

The invention relates to the field of fermentation, and particularly provides a method for fermenting L-lysine. Acetate is added into lysine primary seed culture media, lysine secondary seed culture media and lysine fermentation culture media, and formulas and fermentation processes for the various culture media are optimized. The method has the advantages that growth of lysine producing thalli and synthesis of lysine can be promoted, accordingly, the endpoint lysine content, the total acid content and the saccharic acid conversion rate can be obviously increased, and the fermentation periods can be obviously shortened.
Owner:ANHUI BBCA FERMENTATION TECH ENG RES

Method for increasing yield of L-arginine synthesized from corynebacterium crenatum

The invention discloses a method for increasing the yield of L-arginine synthesized from corynebacterium crenatum, and belongs to the technical field of bioengineering. By means of the method, recombinant corynebacterium crenatum of which a nitrogen transcriptional regulation factor AmtR is knocked out and ammonium transport protein is over-expressed is successfully constructed. By adopting a strategy of batch fermentation by a 5-L fermentation tank and optimizing fermentation conditions, finally the recombinant corynebacterium crenatum Cc5-5 / pXMJ19-amtB is fermented for 96 h, the L-arginine yield of the recombinant corynebacterium crenatum reaches 60.9+ / -1.31 g / L, the yield is increased by 30.56% compared with an original strain of corynebacterium crenatum original strain SYPA5-5, the production intensity reaches 0.634 g / L.h, and the saccharic acid conversion rate is 0.36+ / -0.018 g / g, so that high yield of the L-arginine is realized. Meanwhile, utilization of NH4+ is increased in therecombinant corynebacterium crenatum, so that it is shown that knockout of the nitrogen transcriptional regulation factor and overexpression of the ammonium transport protein have a remarkable effecton the yield of the L-arginine.
Owner:JIANGNAN UNIV

Technique for glycosylated degradation of white spirit waste lees to prepare fermentalbe sugar

The invention relates to a technique for glycosylated degradation of white spirit waste lees to prepare fermentalbe sugar, which includes the procedures of drying and grinding of the white spirit waste lees, glycosylated degradation, the separation of saccharic acid, and the like. The technique is as follows: mixing ground waste lees with hydrogen chloride with mass fraction of 2% according to mass per unit volume of 1:10 for glycosylated degradation and collecting the filtrate; letting the filtrate pass through a chromatographic column which is filled with anionic resin, eluting through distilled water and collecting the eluate by step; and combining the eluates with sugar content equal to or more than 0.5 to be recovered sugar liquid. The recovered sugar liquid can be used as material for alcohol fermentation or other fermentations after being concentrated, and the used anionic exchange resin can be regenerated through NaOH. The concentration of the reducing sugar in the recovered sugar liquid prepared through the technique is equal to or more than 1%, wherein the content of dextrose is 55%, the content of xylose is 35% and the content of others is 10%; and the separation and recovery rate of the saccharic acid is more than 75%. The saccharic acid separation process is low in cost and no harmful impurities such as sulfate radical and the like are left behind, so the fermentalbe sugar has little effect to the subsequent alcohol fermentation or other fermentations. Therefore, the fermentalbe sugar is ideal material for alcohol fermentation and other fermentations.
Owner:SICHUAN UNIV

Escherichia coli capable of producing L-alanine through fermentation and application of escherichia coli

The invention discloses escherichia coli capable of producing L-alanine through fermentation and application of the escherichia coli and belongs to the technical field of biological engineering. According to the escherichia coli, aspartase AspA and L-aspartic acid-beta-decarboxylase AsD are introduced into the escherichia coli capable of producing fumaric acid through the fermentation; furthermore, a YgaW gene is over-expressed to strengthen the transport ability of the L-alanine in recombinant bacteria, so that a novel strain capable of fermenting glucose to produce the L-alanine is constructed; when the recombinant strain is fermented in a fermentation tank for 45h, the yield of the L-alanine reaches 147g / L and the saccharic acid conversion rate is 79.0 percent.
Owner:金华利家园生物工程有限公司 +1

Actinobacillus succinogenes capable of producing succinic acid

The invention discloses actinobacillus succionogenes capable of producing succinic acid with high yield and a method for screening and producing the succinic acid by a fermentation method. The actinobacillus succinogenes takes CGMCC1593 as the original strain and is obtained by blending a plurality of turns of protoplast in a progressive way through a method of '96-pore plate culture, HPLC concentration detection and then anaerobic bottle re-screening'. The actinobacillus succinogenes has already been preserved on February 26, 2012 in China Center for Type Culture Collection with the preservation number CCTCCNO: M2012036. The actinobacillus succinogenes adopts fed-batch culture in a 5 to 15 L fermentation tank, 95.6 g / L succinic acid is produced in 48 hours, the production intensity is 1.99 g / (L.h), and the saccharic acid transformation rate is 0.71 g / g. Compared with other bacterial strains at home and abroad, the actinobacillus succinogenes has the advantages of high yield and lowerproduction cost.
Owner:JIANGNAN UNIV

Corynebacterium-glutamicum recombinant strain for increasing conversion rate of L-phenylalanine saccharic acid

InactiveCN104560852ABlock formationBlock formation pathwayBacteriaTransferasesSaccharic acidMetabolite
The invention discloses a corynebacterium-glutamicum recombinant strain for increasing the conversion rate of L-phenylalanine saccharic acid and belongs to the field of metabolic engineering. The recombinant strain is characterized in that genes such as aroP, aceE, ldh and pstI are deficient, iolT2-ppgk gene is integrated on the original pstI site, and simultaneously regulatory expression plasmid containing eight key enzymes in the L-Phe synthesis path is transferred. The corynebacterium-glutamicum recombinant strain disclosed by the invention has the advantages that the accumulated amount of PEP (phosphoenolpyruvate) of an L-Phe intermediate product, the yield of the L-Phe and the conversion rate of the L-Phe for saccharic acid of glucose are obviously increased, the flowing direction of a carbon source is changed to reduce or block accumulation of side products, and the means of the metabolic engineering can be applied in synthesis of other metabolites adopting PEP as a precursor.
Owner:JIANGNAN UNIV +1

Method for improving saccharic acid conversion rate in L-tryptophan fermentation process

The invention relates to an improved method for preparing L-tryptophan through a fermentation process, and concretely relates to a method for improving the saccharic acid conversion rate in an L-tryptophan fermentation process. In the invention, the content of acetic acid in the fermentation process is substantially reduced by controlling the dissolved oxygen level, the initial glucose concentration, the glucose limited feeding, the specific thallus growth rate and the like according to a cellar metabolic flux distribution regulating principle, so the thallus biomass, the L-tryptophan output and the saccharic acid conversion rate are substantially improved. The method effectively increases the conversion rate in the L-tryptophan fermentation process and substantially improves the L-tryptophan output without adding extra apparatuses or manpower investment, and is suitable for the industrialized production.
Owner:TIANJIN UNIV OF SCI & TECH

Citrus crop special-purposed sustained-and-controlled-release formulated fertilizer, and preparation method and application method thereof

The invention provides a citrus special-purposed sustained-and-controlled-release formulated fertilizer, and a preparation method and an application method thereof. The formulated fertilizer comprises the following components according to the total mass of the fertilizer: 12-20% of nitrogen, 8-12% of phosphorus pentoxide, 12-18% of potassium oxide, 1-4% of calcium, 0.3-1.4% of magnesium, 4.8-9.2% of sulfur, 0.05-0.11% of boron, 0.11-0.22% of zinc, and 0.2-1% of a synergist. A total nutrient content of nitrogen, phosphorus pentoxide and potassium oxide is higher than 30%. A total nutrient content of nitrogen, phosphorus pentoxide, potassium oxide, calcium, magnesium, sulfur, boron and zinc is higher than 40%. 70% of nitrogen is provided by an A-type nitrogen fertilizer with a controlled-release period of 3-5 months and a B-type nitrogen fertilizer with a controlled-release period of 7-9 months. The nitrogen content of the A-type nitrogen fertilizer accounts for 20-55% of the total nitrogen content of the A-type nitrogen fertilizer and the B-type nitrogen fertilizer. With the formulated fertilizer, citrus yield can be improved, and citrus fruit soluble solid, sugar-acid ratio and VC content can be improved.
Owner:INST OF SOIL FERTILIZER & RESOURCE ENVIRONMENT JIANGXI ACAD OF AGRI SCI

Method for improving gluconobacter oxydans for producing 2-keto-L-gulconic acid

InactiveCN104673736AImprove heat resistanceConducive to continuous catalytic reactionsBacteriaMicroorganism based processesSaccharic acidCross-link
The invention discloses a method for improving gluconobacter oxydans for producing 2-keto-L-gulconic acid, and belongs to the technical field of genetic engineering. On the basis of genetic engineering transformation, adaptor protein SH3 and ligand protein SH3lig of the adaptor protein, a CutA gene and a key enzyme gene in saccharic acid transformation are subjected to fusion expression, and G.oxydans is transformed to produce 2-KLG in a one-step fermentation manner, so that the yield of 2-KLG is finally increased to be 40.3 g / L, that is, the yield is increased by 24.4% when being compared with that of a one-step engineering bacterium of which CutA is not expressed; in addition, due to the thermal resistance of foreign protein, the thermal resistance of the engineering bacterium is further improved. Enzyme cross-linking inside cells can be achieved by expressing the foreign protein CutA, continuous catalytic reaction of a plurality of enzymes can be facilitated, and the method has significance in is one-step fermentation production of vitamin C.
Owner:JIANGNAN UNIV

Method of co-catalytically synthesizing various saccharic acids by virtue of synergism of metal ions and selective regulation whole-cell

InactiveCN105132476ADeter and reduce utilizationEfficient synthesisFermentationSaccharic acidCellulose
The invention discloses a method of co-catalytically synthesizing various saccharic acids by virtue of the synergism of metal ions and a selective regulation whole-cell, and relates to the technical field of synthesizing the saccharic acids by biologically catalyzing sugar. The method is mainly characterized in that in a mixed sugar solution or lignocelluloses hydrolysate containing glucose and xylose, 1g / L to 10g / L of gluconobacter oxydans is used as a biological catalyst to co-catalyze the glucose and the xylose under an oxygen-supply condition so as to synthesize the saccharic acids. Metal salt of a given concentration containing zinc ions and trivalent iron ions is added so as to selectively inhibit the catabolism of a cell on the gluconic acid, but a dehydrogenation catalytic reaction of the xylose and the glucose is hardly affected, thus an effect of the cell for co-catalyzing and high-efficiently synthesizing a gluconic acid (salt) and xylonic acid (salt) product can be further achieved, and the reaction time is effectively shortened. By adopting the method, the utilization rate of the glucose and the xylose reaches 100 percent, and the yield of the gluconic acid is more than 70 percent, and the yield of the xylonic acid is more than 92 percent, and the total concentration (mass concentration) of the product gluconic acid (salt) and xylonic acid (salt) can be more than 30 percent.
Owner:NANJING FORESTRY UNIV
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