Method for synchronously determining monosaccharide, uronic acid and saccharic acid in wood fiber material reaction system

A technology of lignocellulosic raw materials and reaction systems, which is applied in the directions of measuring devices, material separation, and analysis of materials, etc., can solve the problem that it is difficult to distinguish the mutual interference of various sugar acids and uronic acids, the inaccurate determination of sugar acids and uronic acids, and the sensitivity Low-level problems, achieve good economic benefits and social effects, improve separation and detection efficiency, and achieve rapid qualitative analysis

Active Publication Date: 2012-07-18
NANJING FORESTRY UNIV
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  • Claims
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AI Technical Summary

Problems solved by technology

Among these methods, method (1) generally can only measure monosaccharides in the biorefining process of lignocellulosic raw materials; method (2) and method (3) are difficult to distinguish the concentration of various sugar acids and uronic acids in the sample. Mutual interference, inaccurate determination of trace amounts of sugar and uronic acids, and often requires derivatization of the sample
The above-mentioned methods for the determination of lignocellulosic raw material pretreatment liquids all have the disadvantages of cumbersome operation steps, low separation degree of sugar components, poor reproducibility of results, low sensitivity or high detection cost, and cannot be completed simultaneously.

Method used

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  • Method for synchronously determining monosaccharide, uronic acid and saccharic acid in wood fiber material reaction system
  • Method for synchronously determining monosaccharide, uronic acid and saccharic acid in wood fiber material reaction system
  • Method for synchronously determining monosaccharide, uronic acid and saccharic acid in wood fiber material reaction system

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Embodiment 1

[0033] High performance liquid phase ion exchange chromatography system: American Dionex ICS-3000 ion chromatography system, equipped with double pump (DP) built-in vacuum degassing module, electrochemical detector (ED) and automatic sampler (AS40), operating software of chromatography system Chromeleon 6.70 chromatographic workstation was used; chromatographic conditions: chromatographic column: CarboPacTM PA10 (2×250mm) chromatographic column with guard column (2×50mm), column temperature: 30°C; injection volume: 10.0 μL;

[0034] Elution conditions: Binary gradient elution with 18mmol / L sodium hydroxide, 200mmol / L sodium hydroxide and 500mmol / L sodium acetate as the eluent, the flow rate is 0.3mL / min, and 18mmol / L L sodium hydroxide solution for isocratic elution; the concentration gradient of sodium acetate solution elution within 10-20min is 50-200mmol / L, and the concentration gradient of sodium hydroxide solution elution is 65.4-70.8mmol / L; 20-40min The concentration of ...

Embodiment 2

[0060] The lignocellulosic raw material was subjected to steam explosion (200°C, 5.0min) and enzymatic hydrolysis (substrate mass concentration 10%, cellulase dosage 3.0FPIU / g cellulose, xylanase dosage 100IU / g xylan, stirred at 50°C Reaction 24h) After the biorefining process, the sample solution of the reaction system was obtained, the sample solution to be tested was fully dissolved in distilled water at 25-30°C, the volume was fixed and the concentration of the xylo-oligosaccharide component to be tested was adjusted to 0.1-10.0 mg / L, centrifugal 5min under the condition of 10000rpm, filter the supernatant liquid with 0.2 μm microfiltration membrane to obtain sample liquid, transfer to the high performance liquid phase ion exchange chromatography system of embodiment 1 again, adopt CarboPacTM PA10 (2 * 250mm) chromatographic column , the column temperature is 30°C, the injection volume is 10.0 μL, and the binary gradient elution is carried out with 18mmol / L sodium hydroxide...

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Abstract

The invention discloses a method for synchronously determining monosaccharide, uronic acid and saccharic acid in a wood fiber material reaction system. The method comprises the steps as follows: pretreating the wood fiber material reaction system to obtain a solution to be determined; determining standard samples of monosaccharide, uronic acid and saccharic acid by adopting an integrated pulse amperometric detection method and a chromatographic peak integration method and utilizing a high-performance liquid ion exchange chromatograph, thus obtaining a standard equation; determining the solution to be determined by utilizing the high-performance liquid ion exchange chromatograph and calculating the content of each component by utilizing the standard equation; and separating and quantifyingxylose and mannose in the solution to be determined by utilizing a high-performance liquid chromatography, and then amending the result of the high-performance liquid ion exchange chromatography. Themethod for synchronously, correctly and quantitatively determining the monosaccharide, the uronic acid and the saccharic acid is established for the first time, the separation degrees and the detection efficiencies of eight types of materials are improved greatly and the synchronous, correct and quantitative determination on nine types of materials in the wood fiber material reaction system can be realized under the combination of the high-performance liquid ion exchange chromatography; and the method has significance to matter changing, and component analysis and determination on products and intermediate products in bio-refinery process of the wood fiber material.

Description

technical field [0001] The invention relates to a method for synchronous analysis and detection of various products and intermediate products produced in the biorefining process of lignocellulosic raw materials, in particular to a method for the simultaneous determination of various monosaccharides, uronic acids and sugar acids by using high performance liquid phase ion exchange chromatography The method, in particular to a method for the simultaneous determination of arabinose, galactose, glucose, xylose, xylonic acid, gluconic acid, galacturonic acid and glucuronic acid produced in the biorefining process of lignocellulosic raw materials by using high performance liquid phase ion exchange chromatography The composition and content of substances such as methods. Background technique [0002] The biorefining of lignocellulosic raw materials is a current hotspot. The basic composition of raw materials and their material changes during biological or chemical processing are bas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 徐勇余世袁勇强王荥朱均均李鑫周晓青连之娜
Owner NANJING FORESTRY UNIV
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