310 results about "Branched-chain amino acid" patented technology

The invention provides a nursing sow high-efficiency feed and a preparation method thereof. Raw materials such as corn, white sugar, wheat bran, fish meal, soya bean meal, puffed soybean, feather meal, blood globulin powder, distillers dried grains with soluble (DDGS), soybean oil, vitamine, trace elements, an ionic equilibrium agent, a physiological regulator and the like are adopted, and the nursing sow high-efficiency feed is prepared by performing smashing, mixing, tampering, pelletizing and other processing on the raw materials. In the invention, the newest theory of the existing animal nutrition and feeding is taken as a basis and production practice is combined, thus the defects of the prior art are overcome. The high-efficiency feed has the following advantages: the current cultivation situation in the southern part of China, market requirements and the newest sow nutrition theory are combined, and maximization of productive potential of a nursing sow is taken as an aim, especially amino acid balance is considered, so that branched chain amino acid nutrition is enriched, the sow antistress capability is improved, and the palatability of the feed is improved; and the ionic balance of the feed is considered, high-quality feed raw materials are selected, and strict scientific processing is carried out, thus the milk production performance of the nursing sow can be greatly improved, finally the weight of weaning litter of suckling pigs is improved, and the market requirement is met.

A composition includes a plurality of amino acids. The plurality of amino acids includes at least one essential amino acid and at least one non-essential amino acid. The plurality of amino acids also includes at least one branch-chain amino acid. The composition also includes a source of carbohydrates. The compositions also includes purified water. The plurality of amino acids comprises about 1 wt % of the composition. A composition includes a plurality of amino acids, sodium citrate, sodium chloride, potassium phosphate, flavoring, a source of carbohydrates, and purified water. In some embodiments of the composition, the plurality of amino acids includes alanine, arginine, aspartate, cystine, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonin, tryptophan, tyrosine, and valine.

An object of the present invention is to provide: a novel gene (polynucleotide) encoding an FAD-conjugated glucose dehydrogenase having excellent properties that it has excellent reactivity to glucose, excellent thermal stability, and excellent substrate-recognition performance and also has a low activity for maltose; a process for the production of the enzyme using a transformant cell transfected with the gene; and a method for the determination of glucose, a reagent composition for use in the determination of glucose, a biosensor for use in the determination of glucose and others, each characterized by using the enzyme obtained. The invention relates to a polynucleotide encoding an FAD-conjugated glucose dehydrogenase, comprising a polypeptide containing an amino acid sequence: X1-X2-X3-X4-X5-X6 (wherein X1 and X2 independently represent an aliphatic amino acid residue; X3 and X6 independently represent a branched amino acid residue; and X4 and X5 independently represent a heterocyclic amino acid residue or an aromatic amino acid residue); and others.

The present invention relates to mutant microorganisms having improved productivity of branched-chain amino acids, and a method for producing branched-chain amino acids using the mutant microorganisms. More specifically, relates to mutant microorganisms having improved productivity of L-valine, which are produced by attenuating or deleting a gene encoding an enzyme involved in L-isoleucine biosynthesis, a gene encoding an enzyme involved in L-leucine, and a gene encoding an enzyme involved in D-pantothenic acid biosynthesis, and mutating a gene encoding an enzyme involved in L-valine biosynthesis, such that the expression thereof is increased, as well as a method for producing L-valine using the mutant microorganisms. The inventive mutant microorganisms produced by site-specific mutagenesis and metabolic pathway engineering can produce branched-chain amino acids, particularly L-valine, with high efficiency, and thus will be useful as industrial microorganisms for producing L-valine.

A method of modulating plasma levels of branched chain amino acids and branched chain alpha-keto acids is disclosed, wherein an ammonia scavenger compound or a salt thereof, for example phenylbutyrate or an even numbered congener thereof or a salt thereof, is administered to an individual in need thereof. In various methods, a decrease in plasma levels of branched chain amino acids and branched chain alpha-keto acids is effected to treat individuals suffering from an inborn error in metabolism of amino acids, such as Maple Syrup Urine Disease, for example.

The invention provides methods for treating muscle loss in an individual. In one embodiment, the invention includes administering to an individual an effective amount of a branched chain amino acid (BCAA), a BCAA precursor, a BCAA metabolite, a BCAA-rich protein, a protein manipulated to enrich the BCAA content or any combination thereof. The invention further provides nutritional products for such administration, including orally-administrable nutritional products

The invention relates to a high-protein dietary fiber energy protein bar and a production method thereof. The protein bar comprises the following materials in parts by weight: 5-35 parts of soy protein, 0-31 parts of cocoa butter replacer chocolate, 3-29 parts of glucose syrup, 0-18 parts of oligomeric isomaltose, 3-8 parts of concentrated whey protein powder and 3-9 parts of plaint oil. According to the formula of the protein bar, the soy protein, the concentrated whey protein, the glucose syrup and the oligomeric isomaltose are used as main raw materials for simultaneously supplementing exercising people with protein and energy (carbohydrate). The added concentrated whey protein has nutrient substances such as high-quality alpha-lactalbumin, beta-lactoglobulin and branched chain amino acid, thus being beneficial to rapidly supplementing protein after movement and relieving fatigue; the protein, energy (carbohydrate) and functional nutrient substances (such as dietary fiber) are rapidly supplemented to the exercising people; the protein bar provided by the invention is easy to carry, convenient to eat and good in taste.

The invention provides a genetically engineered bacterium for high-yielding L-valine. A construction method of the genetically engineered bacterium comprises the steps that starting from an escherichia coli W3110, an acetolactate synthase gene alsS of a bacillus subtilis is integrated on a genome of the escherichia coli W3110 and subjected to high expression; an escherichia coli ppGpp 3'-pyrophosphoric acid hydrolytic enzyme mutant R290E / K292D gene spoT is integrated on the genome of the escherichia coli W3110 and subjected to high expression; genes of frdA, frdB, frdC and frdD of four subunits of a lactic dehydrogenase gene ldhA, a pyruvate formate lyase I gene pflB and fumaric reductase on the genome of the escherichia coli W3110 are knocked out; a branched chain amino acid transaminasegene ilvE of the escherichia coli is replaced with leucine dehydrogenase gene bcd of the bacillus subtilis; and an acetyl-hydroxyl acid isomerized reductase gene ilvC of the escherichia coli is replaced with an encoding gene of a mutant L67E / R68F / K75E. According to the genetically engineered bacterium for the high-yielding L-valine, an L-valine fermentation method is further modified. Double-phasedissolved oxygen control is adopted, and the L-valine yield and the saccharic acid conversion rate are improved.

An object of the present invention is to provide: a novel gene (polynucleotide) encoding an FAD-conjugated glucose dehydrogenase having excellent properties that it has excellent reactivity to glucose, excellent thermal stability, and excellent substrate-recognition performance and also has a low activity for maltose; a process for the production of the enzyme using a transformant cell transfected with the gene; and a method for the determination of glucose, a reagent composition for use in the determination of glucose, a biosensor for use in the determination of glucose and others, each characterized by using the enzyme obtained. The invention relates to a polynucleotide encoding an FAD-conjugated glucose dehydrogenase, comprising a polypeptide containing an amino acid sequence: X1-X2-X3-X4-X5-X6 (wherein X1 and X2 independently represent an aliphatic amino acid residue; X3 and X6 independently represent a branched amino acid residue; and X4 and X5 independently represent a heterocyclic amino acid residue or an aromatic amino acid residue); and others.

The invention discloses a method for preparing pinctada martensii high F value oligopeptide by an enzymatic method, relates to enzymatic hydrolysis of a pinctada martensii meat protein and desorption of an aromatic amino acid in a hydrolysate by adopting an effective separation means, and provides a method for preparing an oligopeptide mixture with the molecular weight of less than 2,000 Da and the F value (the molar ratio of a branched chain amino acid to the aromatic amino acid) of more than 20 by adopting the enzymatic hydrolysis of the pinctada martensii meat protein and an adsorbent adsorption and separation desorption effect. In the method, an aquatic animal protein is first taken as a raw material to prepare the high F value oligopeptide; the raw material is extracted by using hot water in a preparation process, the extract can be used for extracting taurine, and the precipitated protein is taken as an enzymatic substrate, so that the resource is utilized fully and effectively; a single enzyme, namely pancreatin is used as the preferred active enzyme; and the aromatic amino acid is desorbed by adopting a method of combining activated carbon static adsorption and gel filtration chromatography to prepare a high F value oligopeptide product.

Methods useful for reducing or preventing non-alcoholic steatohepatitis or hepatic steatosis are provided herein. Such methods may comprise administering to a subject in need thereof a sirtuin pathway activator and / or PDE5 inhibitor alone or in combination with an amount of a branched amino acid in free amino acid form, or a metabolite thereof. Also provided herein are compositions and kits for practicing any of the methods described herein.

The invention discloses a corynebacteria expression system without depending on antibiotic being selection pressure, and belongs to the microbe gene engineering field. Alr knock out carrier and expression carrier used for plasmid anaplerosis are constructed to bring conveniences for lab research and utilization, and a fermentation process no longer needs antibiotic. Through test results, the expression system realizes thalline inner plasmid stability in fermentation without adding the antibiotic; when the expression system is used to construct overexpression vector of branched chain amino acid-valine synthesis path main gene ilvBNC, valine output can be obviously improved; the corynebacteria expression system has wide application value.

The present invention provides a fatigue-preventive (brain fatigue) agent or a fatigue-recovering agent for the central nervous system, which contains a branched-chain amino acid, such as L-valine, L-leucine and L-isoleucine, and / or a 2-aminobicyclo-[2,2,1]-heptane-2-carboxylic acid. In addition to the application as injection and transfusion, these can be prepared as solid-state modes to be taken, such as tablets, pellets and powder medicine by adding an appropriate vehicle such as starch and lactose thereto. Moreover, these can be prepared as various beverages such as so-called health drinks and sports drinks, or as food items for fatigue-recovering and the fatigue-prevention in the central nervous system.

The present invention provides for compositions, methods and kits for regulating energy metabolism. In one aspect, the invention provides for compositions that comprise a combination of (a) branched chain amino acids, such as leucine, and (b) vitamin B6, or any precursors or metabolites of (a) or (b). These combinations may be synergistic and / or effective for reducing weight or adipose volume. In another aspect, the invention provides for methods of regulating energy metabolism by the administration of one or more compositions comprising branched chain amino acids and vitamin B6. The invention also provides for kits comprising compositions of branched chain amino acids and vitamin B6 packaged in an oral dose form with usage instructions.

The invention relates to the formula of a special culture material capable of increasing the content of each amino acid in a grey mushroom fruiting body and a culture method. The formula provided by the invention is characterized in that the formula of the special culture material consists of the following raw materials in part by weight: 20 to 30 parts of straw, 20 to 30 parts of sawdust, 20 to 27 parts of cottonseed hull, 10 to 15 parts of bran, 0.3 to 0.5 part of monopotassium phosphate, 0.2 to 0.3 part of magnesium sulfate and 1.0 to 1.2 parts of calcium carbonate or calcium sulfate, wherein 10 to 30 kilos of pig excrement residues are added into 100 kilos of mixed culture material. According to the invention, the grey mushroom fruiting body has a round and attractive mushroom flower and greatly increases the content of each amino acid in the grey mushroom fruiting body, wherein the content of sulfur amino acid, branched chain amino acid, children amino acid, essential amino acid, flavor amino acid and sweet amino acid is greatly increased respectively. The grey mushroom fruiting body can increase the health-care medicinal function, increase the production additional value, reduce pollutants in the pig breeding industry and recycle the pig excrement residues.

The invention relates to a recombinant DNA (deoxyribonucleic acid), strain and method for producing L-valine by fermentation. The recombinant DNA comprises DNA sequence for coding acetohydroxy acid synthetase free of feedback inhibition of three branched-chain amino acids to acetohydroxy acid synthetase, DNA sequence for coding dihydroxy reduction isomerase, and DNA sequence for coding branched-chain amino acid aminotransferase. The strain is corynebacterium or brevibacterium transformed by introducing the recombinant DNA. The method is implemented by culturing the strain to produce the L-valine. In the invention, the expression L-valine is used for producing the bacterium L-valine biosynthetic gene, so the yield of the L-valine can be greatly increased; and the fermentation model, especially the high-temperature short-time fermentation model, is constructed according to the optimum temperature of the L-valine biosynthetic enzyme, so that the metabolism intensity of the strain is enhanced, the activity of the L-valine biosynthetic enzyme is enhanced, and the yield of the L-valine and the conversion rate of the sugar acid can be further increased.

The invention provides a sport nutrition solid drink. The component of the sport nutrition solid drink comprises ginseng powder, an oligopeptide, whey protein powder, an inulin, a taurine, a branched chain amino acid, whole milk powder and a food additive. The beneficial effect of the sport nutrition solid drink is that the ginseng powder (artificial cultivation), the oligopeptide, the whey protein powder, a glutamine, and the branched chain amino acid are matched up so that the function of each component can be effectively acted, the synergistic effect between components is used better, the sports fatigue is effectively relieved, the sport injury is prevented, and the immunity of the organism is enhanced. The invention further provides a preparation method based on the sport nutrition solid drink.

The invention discloses an anti-hypoxia and anti-fatigue energy composition which comprises puffing black rice flour, oatmeal, coconut oil, corn syrup, isolated soy protein powder, maltodextrin, carnitine, a vine tea and flavone extract, a grapeseed extract, a quercetin extract, a silverweed cinquefoil root extract, a rhodiola extract, octacosane alkanol, branched chain amino acid, taurine, low-fat coconut powder and concentrated vanilla, wherein the multiple components are cooperated. The composition can be used for improving the aerobic metabolism of an organism, improving the oxygen utilization rate of cells, enhancing the plateau adaptiveness and improving the working capacity under a plateau low-oxygen condition; furthermore, the composition can quickly supplement sugar, fat, proteins, electrolyte and other nutrients, which are needed by the organism, and promote absorption of nutrition and can also quickly complement the carbohydrates and the nutrients, which are consumed and lost during motion, particularly in high-intensity sports training and fitness, of a human body, so that fatigues of central nervous system and bones can be relieved, and toxic and side effects are avoided.

The invention belongs to the medicine field, and specifically relates to an intra-intestinal nutrient emulsion for tumor patients. The emulsion is characterized by comprises the following effective components: protein, fat, carbohydrate, total dietary fiber, green tea theine, taurine, L-carnitine, composite vitamins and mineral substances, and water. The protein is provided in an amino acid powder form, the formula of the amino acid powder is adjusted on the basis of 20 kinds of protein amino acids, wherein methionine is replaced by homocysteine, glutamine and glutamic acid are not added, the tryptophan content is low, and the content of branched chain amino acid reaches 35% or more; fat is provided in a saturated aliphatic acid or unsaturated fatty acid form, wherein in the unsaturated aliphatic acid the value of omega-6 / omega-3 is equal to 2-6 / 1; and the carbohydrate is provided in a form of glycerin, glucose, fructo-oligosaccharide or lentinan. Besides one prominent characteristic of the emulsion is that the emulsion is a high-fat and low-sugar type, and the ratio of sugar to fat is 1:1. The emulsion formula is adjusted according to the nutrient and metabolism requirements of tumor patients so as to develop an individualized intra-intestinal nutrient preparation for tumor patients, the preparation can provide balanced nutrients for tumor patients, effectively cures the malnutrition symptom, improves the life quality of patients, and can also be used to assist the tumor treatment.

The invention provides an orally-administrable nutritional product comprising a dipeptide including a branched chain amino acid (BCAA). In one embodiment, the nutritional product comprises a dipeptide selected from at least one of the following: alanyl-leucine, alanyl-isoleucine, alanyl-valine, glycyl-leucine, glycyl-isoleucine, and glycyl-valine.

The present invention comprises a compound amino acid injection and the preparation method. The characteristics of the injection are as follows. The prescription comprises most human essential amino acid and non essential amino acid. Content of branched chain amino acid (BCAA) in each amino acid is high, and content of the aromatic amino acid (AAA) is low. Content of arginine is high. The prescription comprises the acetylcysteine. The compound amino acid injection is prepared under strict control of aerobic environment, which makes the product in accordance with quality requirement without any antioxidant. The production is applicable in the patients with hepatic disease, which is in accordance with specific requirement for AAA restriction. No antioxidant is added in the prescription, which can effectively reduce incidence rate of adverse reaction in clinical application.

The invention provides a microencapsulated amino-acid composition and a preparation method of the microencapsulated amino-acid composition. The microencapsulated amino-acid composition can be dispersed quickly and dissolved clearly. The preparation method of the microencapsulated amino acid composition comprises the steps that auxiliary materials such as a coating agent and a wetting agent are added to an amino-acid mixture, and the wetting speed of composition particles to water is increased greatly by surface modification of microencapsulated crumby particles. A mass ratio of the amino acid to the auxiliary materials in the composition is 100 / 0.1-10.0. Compared with the conventional product, the composition has better dispersing and fast dissolving properties in the water, and a solution is transparent and free of sediment, floatage and foam. The shelf life of the composition is stable, the composition can be used more conveniently and rapidly, facilitates fast absorption of human bodies, and solves the problems that edible demands of humans cannot be met, the amino-acid is difficult to take and absorb, and taste and odor of the amino-acid get worse as branched-chain amino acid in the amino-acid is low in dissolvability and poor in hydrophilicity and stability. The preparation method is simple in technology, and facilitates the industrial production.

The invention discloses an anti-fatigue energy composition which is prepared by the following raw materials in parts by weight: expanded black rice flour, oatmeal, coconut oil, corn syrup, soybean protein isolate powder, maltodextrin, carnitine, vine tea flavone extract, grape seed extract, quercetin extract, octacosanol, caffeine, branched chain amino acid, taurine, low fat cocoa and concentrated vanilla; the anti-fatigue energy composition is capable of rapidly supplementing sugar, fat, protein, electrolyte and other nutrients which are needed by the organism and promoting the nutrients to be absorbed, can be used for rapidly supplementing the consumed carbohydrate and the lost nutrients of human body caused by sweating excessively under the condition of exercises, especially medium and high intensity exercises and exercise training, and is beneficial for relieving central and peripheral fatigue; after the anti-fatigue energy composition is adopted, the antioxidant capacity and the aerobic metabolism level of the organism are improved, the oxygen utilization rate of cells can be improved, the moment and fatigue resistance of the organism can be better rapidly improved, and the sport injury is avoided.

The invention relates to BCAT inhibitors and the use thereof for treating or preventing neuronal loss associated with stroke, ischemia, CNS trauma, hypoglycemia and surgery, as well as treating neurodegenerative diseases including Alzheimer's disease, amyotrophic lateral sclerosis, Huntington's disease and Down's syndrome, treating or preventing the adverse consequences of the overstimulation of the excitatory amino acids, treating anxiety, psychosis, convulsions, aminoglycoside antibiotics-induced hearing loss, migraine headache, chronic pain, neuropathic pain, Parkinson's disease, diabetic retinopathy, glaucoma, CMV retinitis, urinary incontinence, opioid tolerance or withdrawal, and inducing anesthesia, as well as for enhancing cognition.

A prophylactic or therapeutic composition for hemoglobinuria or myoglobinuria which may occur in humans or animals upon loading of exercise or stress or the like is provided.According to the present invention, the prophylactic or therapeutic composition for hemoglobinuria or myoglobinuria which comprises a branched-chain amino acid such as valine, leucine or isoleucine or a salt thereof, a basic amino acid such as ornithine, arginine, lysine, histidine or citrulline or a salt thereof and glutamine or a salt thereof as active ingredients can be provided.

The invention discloses high-F-ratio leech oligopeptide and an enzymatic hydrolysis preparation method and application thereof. The preparation method comprises the following steps: (1) preliminarily cleaning dried leech bodies, removing impurities and carrying out high-speed crushing; (2) pre-soaking raw materials with alkali; (3) carrying out enzymatic hydrolysis by using double enzymes consisting of alkali protease AlcalaseAF 2.4L and papain; (4) statically adsorbing and removing aromatic amino acids by using active carbon powder; and (5) carrying out further separation and purification by using a gel filtration chromatographic column, collecting a separation product and then carrying out freeze drying so as to obtain leech oligopeptide powder with a molecular weight of less than 2000 Da and an F ratio (Fishcer ratio, i.e., a ratio of the mole content of branched-chain amino acids to the mole content of aromatic amino acids) of more than 20. According to the invention, preparation process is simple, two commercial enzymes consisting of alkali protease and papain are selected and used, industrial production can be easily realized, high yield is obtained, and the high-F-ratio leech oligopeptide has a certain anticoagulation effect and increases a novel source for high-F-ratio products.

A composition of dipeptides or tripeptides or ester derivatives may be used as a nutritional supplement and to modulate expression of genes associated with healthy maintenance and regulation of muscular and neurological tissue. The compositions include dipeptides and / or tripeptides of branched chain amino acids and optionally include adjuvants, anti-aging compounds and may be provided in a dissolvable powder or liquid form.