A stem cell culture solution is prepared from raw materials as follows: 10% of a compound amino acid injection, 1% of water-soluble vitamins for injection, 0.5% of glutamine dipeptide, 10% of human albumin, 10% of a hydrolyzed protein injection, 0.5% of a microelement injection and 68% of a plasmalyte-A solution, wherein the pH value is 6.8-7.0, and the osmotic pressure is 310 mOsm / kg. The stem cell culture solution is used for culturing mesenchymal stem cells, and a mesenchymal stem cell culture supernatant is collected; the collected culture supernatant is filtered by a filter membrane, and filtrate is collected and is the stem cell injection. A decellularized stem cell secretion factor is used for intravenous injection, so that the dispute of stem cells in the aspect of ethic is effectively avoided; the stem cell culture solution is prepared from clinical raw materials, is safe and reliable, provides nutrition for the living environment of the stem cells, can effectively promote the stem cells to secrete a large quantity of cell factors and keeps the activity of the stem cells; through cooperation of a nutritional supplement formula and the stem cell secretion factor, the sub-health state of a body can be effectively relieved, and the body health is regained.