The invention discloses a serum-free medium for mesenchymal stem cells, which comprises a basal medium and supplementary components added to the basal medium, the supplementary components include L-glutamine, non-essential amino acids, L-ascorbic acid, Sodium selenate, fibronectin, ethanolamine, hydrocortisone, trypsin inhibitor, human transferrin, human insulin, bFGF, TGF‑β1 and PDGF‑BB, this medium overcomes the serum-free culture in the prior art The base has poor cell adhesion, relatively complex components, and does not support primary cell culture.