41results about How to "Promotes adherent growth" patented technology

The invention discloses a serum-free medium for mesenchymal stem cells, which comprises a basal medium and supplementary components added to the basal medium, the supplementary components include L-glutamine, non-essential amino acids, L-ascorbic acid, Sodium selenate, fibronectin, ethanolamine, hydrocortisone, trypsin inhibitor, human transferrin, human insulin, bFGF, TGF‑β1 and PDGF‑BB, this medium overcomes the serum-free culture in the prior art The base has poor cell adhesion, relatively complex components, and does not support primary cell culture.

The invention discloses a plush-type bioreactor artificial liver system. The plush-type bioreactor artificial liver system comprises a plasma separating / recirculating part and a bioreactor circulating part which are mutually connected through a pipe, wherein the plasma separating / recirculating part comprises a blood transfusion port, a blood pump, an arterial ampulla, a plasma separator, a venous ampulla and a blood retransfusion port which are sequentially connected; a blood inlet, a plasma outlet and a blood cell outlet are formed in the plasma separator; the blood inlet is communicated with the outlet of the arterial ampulla; an artificial liver bioreactor comprises a reactor housing, and a plurality of pieces of plush and a plurality of hollow fiber tubes, which are arranged inside the inner chamber of the reactor housing; the plurality of pieces of plush and the plurality of hollow fiber tubes are arranged in parallel and are alternately spaced. The plush-type bioreactor artificial liver system has the beneficial effects as follows: the plush-type bioreactor artificial liver system significantly increases the adherent area of liver cells in the reactor, improves the uniformity of liver cell distribution, can maintain good cell activity, and benefits maximum achievement of functions of the liver cells, so that the best therapeutic effect is achieved.

The invention discloses collagen-based composite hemostasis powder and a preparation method thereof. The method is characterized by comprising the following steps: modifying type I collagen by using dencichine, preparing the modified type I collagen into a solution with the concentration of 1-2 percent by using polylysine-modified chitosan; preparing medical gelatin into a 1-3% solution; preparing the modified chitosan into a 1-3% solution; blending the three solutions according to a mass ratio of 0.5-1:2-8:8-2; stirring at the temperature of 4-10 DEG C for 8-10 hours, and performing freeze drying to prepare composite sponge; sequentially impregnating the composite sponge in a modifier and a blood coagulation factor in an ultrasonic cleaner, fully washing, performing freeze drying, and grinding by a grinder to prepare the collagen-based composite hemostasis powder. The powder has the effects of rapidly and effectively stopping bleeding, resisting bacteria, diminishing inflammation, relieving pain, promoting wound healing and repairing, has high adhesion property, hydrophilicity, biological degradability and biocompatibility and is low in price and suitable for wound hemostasis or repair.

The invention provides a rapid preparation of accellular porcine intestinal submucosa. The method sequentially comprises the steps of decellularization treatment performed before degreasing treatment,the degreasing treatment, and secondary decellularization treatment and renaturation treatment which are performed after the degreasing treatment. The rapid preparation of the accellular porcine intestinal submucosa not only can quickly and effectively remove all relevant antigen components of all cells and the like in tissues, but also maintains the good chemical composition and mechanical properties of extracellular matrix (ECM).

The invention discloses a four-in-one plush thread type artificial liver bioreactor, which includes a constant temperature water tank and a three-in-one bioreactor placed inside it; the three-in-one bioreactor includes a cavity and is arranged in the cavity The first partition and the second partition in the body; the first partition and the second partition divide the cavity into three independent spaces from left to right, followed by plasma separator, oxygenator and reactor ; The constant temperature water tank includes a water tank shell and a hot water inlet and a hot water outlet located on the water tank shell. The beneficial effects of the present invention are: after the blood enters the plasma separator, the plasma enters the oxygenator and the reactor in turn, reducing unnecessary pipelines; it can provide a suitable metabolic environment for cells and ensure cell activity; it can increase the effective cell filling volume and ensure The cells are evenly distributed laterally.

The invention discloses a manufacturing method for a hard micro-fluid chip. The manufacturing method comprises the following steps: preparing an upper chip and chemically bonding and packaging the upper chip under a semi-cured state with a substrate, thereby acquiring the hard micro-fluid chip. The upper chip can be prepared from epoxy resin or amino resin; when the upper chip is prepared, the heating curing temperature is 45-85 DEG C and the time is 15 minutes to 8 hours; a thermal polymerized epoxy resin material is used after the two materials of prepolymer and curing agent are mixed, the viscosity thereof after mixing is low (approximate to mineral oil viscosity), the rolling printing for a tiny structure at micron scale and even nanometer scale is convenient and the rolling precision is high; the mixture can be solidified within 40min under the temperature of 80 DEG C, the preparation time of the micro-fluid chip is shortened, the period is short, the consumption of a reagent is less and the batch production is convenient; the hard micro-fluid chip can be flexibly packaged and combined with various substrates; the packaging is independent of external high temperature and high pressure environments; and the packaging strength is high and the speed is high.

The invention discloses a bioreactor for in vitro culture of neurotrophic factors, which comprises an upper cover and a lower cover which are provided with concave cavities. The concave cavities of the upper cover and the lower cover are closed in opposite directions and fixed to form a box body, a culture plate through which cerebrospinal fluid can pass is arranged in the cavity of the box body,a polyether sulfone semi-permeable membrane is covered on the culture plate, the culture plate is embedded to the middle lower part of the concave cavity of the lower cover and positioned between theupper cover and the lower cover, both the upper part and the lower part of the culture plate are spaces in which the cerebrospinal fluid flows, the upper cover is provided with an input nozzle, the lower cover is provided with an output nozzle, and the upper end face of the upper cover is provided with a plurality of holes through which the air enters the box body. The bioreactor enables the cerebrospinal fluid of a nervous system dysfunction patient to be circulated in the bioreactor and fully mixed with the neurotrophic factors secreted by star-shaped gliocytes to form mixed cerebrospinal fluid containing the neurotrophic factors to be redelivered to the lower cavity of the arachnoid membrane or the ventricular system of the patient, thereby realizing promotion of recovery of functionaldeficiency or functional disorder of the central nervous system caused by diseases or injury, and remarkably improving the life quality of the patient.

The invention discloses a plush thread type bioreactor artificial liver system, which includes a plasma separation / reinfusion circulation part and a bioreactor circulation part, and the two parts are connected to each other through pipelines; the plasma separation / reinfusion circulation part includes sequentially connected blood Input port, blood pump, arterial jug, plasma separator, venous jug and blood return port; plasma separator is equipped with blood inlet, plasma outlet and blood cell outlet; blood inlet is connected with the outlet of arterial jug; bioartificial liver reaction The reactor comprises a reactor shell and a plurality of plush wires and a plurality of hollow fiber tubes arranged in the inner cavity of the reactor shell; the plurality of plush threads and the plurality of hollow fiber tubes are arranged in parallel and alternately arranged at intervals. The beneficial effects of the present invention are: significantly increasing the area of ​​the hepatocytes that can adhere to the wall in the reactor, improving the uniformity of the distribution of the hepatocytes, maintaining good cell activity, and being conducive to the maximum play of the function of the hepatocytes, thereby achieving the best good therapeutic effect.

The invention discloses a chitosan polyacrylamide hydrogel base material and a preparation method thereof. The chitosan polyacrylamide hydrogel base material is composed of an acrylamide solution, a methylene diacrylamide solution, H2O, an ammonium persulfate solution, tetramethyl ethylenediamine and a chitosan solution. The preparation method of the chitosan polyacrylamide hydrogel base material comprises a step of mixing and stirring, a step of transferring to a glass clamping plate for reacting, and a step of immersion. The chitosan polyacrylamide hydrogel base material has a simple technology and allows hydrogels having different hardnesses to be obtained, and the obtained hydrogels have the advantages of non-toxicity, easy adherence growth of cells, reduction of the reaching complexity of the cytomechanics, benefiting for the researches on the mechanical properties of the cells, and reduction of the researching cost.

The invention discloses a plush thread bioartificial liver reactor, which comprises a reactor shell, a plurality of plush threads and a plurality of hollow fiber tubes arranged in the inner cavity of the reactor shell; one end of the reactor shell is provided with a The plasma / preflush inlet and the nutrient solution / liver cell suspension inlet are provided at the other end with the plasma / preflush outlet and the nutrient solution outlet; the two ends of the hollow fiber tube are respectively connected to the plasma / preflush inlet and the The plasma / preflush outlet is connected; the plurality of plush threads and the plurality of hollow fiber tubes are arranged in parallel and alternately at intervals. The beneficial effects of the present invention are: significantly increasing the area of ​​the hepatocytes that can adhere to the wall in the reactor, improving the uniformity of the distribution of the hepatocytes, maintaining good cell activity, and being conducive to the maximum play of the function of the hepatocytes, thereby achieving the best good therapeutic effect.