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41results about How to "Promotes adherent growth" patented technology
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Mesenchymal stem cell serum-free culture medium
ActiveCN106906182AEasy to add componentsThe added ingredients are clearCulture processSkeletal/connective tissue cellsL-glutamineTransferrin
The invention discloses a mesenchymal stem cell serum-free culture medium. The mesenchymal stem cell serum-free culture medium comprises a basic culture medium and added ingredients added in the basic culture medium, and the added ingredients comprise L-glutamine, nonessential amino acid, L-ascorbic acid, sodium selenite, fibronectin, ethanolamine, hydrocortisone, a trypsin inhibitor, human transferrin, human insulin, bFGF, TGF-beta1 and PDGF-BB. By means of the culture medium, the problems that in the prior art, the serum-free culture medium is poor in cell adherence, components are relatively complex, and primary cell culture is not supported are solved.
Owner:北京赛斯达生物技术有限公司
Chitosan polyacrylamide hydrogel base material and preparation method thereof
The invention discloses a chitosan polyacrylamide hydrogel base material and a preparation method thereof. The chitosan polyacrylamide hydrogel base material is composed of an acrylamide solution, a methylene diacrylamide solution, H2O, an ammonium persulfate solution, tetramethyl ethylenediamine and a chitosan solution. The preparation method of the chitosan polyacrylamide hydrogel base material comprises a step of mixing and stirring, a step of transferring to a glass clamping plate for reacting, and a step of immersion. The chitosan polyacrylamide hydrogel base material has a simple technology and allows hydrogels having different hardnesses to be obtained, and the obtained hydrogels have the advantages of non-toxicity, easy adherence growth of cells, reduction of the reaching complexity of the cytomechanics, benefiting for the researches on the mechanical properties of the cells, and reduction of the researching cost.
Owner:CHONGQING UNIV
Collagen-based composite hemostasis powder and preparation method thereof
ActiveCN103721247AGood hemostatic effectGood biocompatibilityOrganic active ingredientsPeptide/protein ingredientsFreeze-dryingBiocompatibility Testing
The invention discloses collagen-based composite hemostasis powder and a preparation method thereof. The method is characterized by comprising the following steps: modifying type I collagen by using dencichine, preparing the modified type I collagen into a solution with the concentration of 1-2 percent by using polylysine-modified chitosan; preparing medical gelatin into a 1-3% solution; preparing the modified chitosan into a 1-3% solution; blending the three solutions according to a mass ratio of 0.5-1:2-8:8-2; stirring at the temperature of 4-10 DEG C for 8-10 hours, and performing freeze drying to prepare composite sponge; sequentially impregnating the composite sponge in a modifier and a blood coagulation factor in an ultrasonic cleaner, fully washing, performing freeze drying, and grinding by a grinder to prepare the collagen-based composite hemostasis powder. The powder has the effects of rapidly and effectively stopping bleeding, resisting bacteria, diminishing inflammation, relieving pain, promoting wound healing and repairing, has high adhesion property, hydrophilicity, biological degradability and biocompatibility and is low in price and suitable for wound hemostasis or repair.
Owner:BEIJING HOTWIRE MEDICAL TECH DEV CO LTD +1
Preparation method of composite cavity microfibers based on micro-fluidic technology
ActiveCN108149342AStable growthPrecise Density ControlConjugated cellulose/protein artificial filamentsArtificial cell constructsCell adhesionAdhesion process
The invention relates to a preparation method of composite cavity microfibers based on a micro-fluidic technology. The preparation method is characterized in that in the preparation process of cavitymicrofibers, a modified material capable of promoting the cell wall adhesion growth is introduced in a microfiber inner cavity; while the cavity is formed, the modified material is adhered onto the cavity to form a modified coating layer, so that a promoting effect on late cell adhesion and culture is provided. By utilizing the micro-fluidic technology, a micron-sized channel capable of generatinga coaxial laminar flow pattern is formed, the flow pattern control on a sample fluid is realized, and the sample fluid is finally solidified into a micron-sized hollow fiber material with a specificinner coating structure. The microfiber material can simulate a microstructure in a human body tissue, and a new method and a new concept are provided for tissue engineering and organ regeneration. The preparation method provided by the invention is simple and reliable to operate, high in efficiency and excellent in technical effect; convenient conditions are provided for the modification of the microfibers; an internal finish coating is uniform, stable, simple and controllable to facilitate the cell wall adhesion growth.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Manufacturing method for hard micro-fluid chip
ActiveCN107305214AHigh light transmittanceReduced autofluorescenceMaterial strength using tensile/compressive forcesTransmissivity measurementsEpoxyMicron scale
The invention discloses a manufacturing method for a hard micro-fluid chip. The manufacturing method comprises the following steps: preparing an upper chip and chemically bonding and packaging the upper chip under a semi-cured state with a substrate, thereby acquiring the hard micro-fluid chip. The upper chip can be prepared from epoxy resin or amino resin; when the upper chip is prepared, the heating curing temperature is 45-85 DEG C and the time is 15 minutes to 8 hours; a thermal polymerized epoxy resin material is used after the two materials of prepolymer and curing agent are mixed, the viscosity thereof after mixing is low (approximate to mineral oil viscosity), the rolling printing for a tiny structure at micron scale and even nanometer scale is convenient and the rolling precision is high; the mixture can be solidified within 40min under the temperature of 80 DEG C, the preparation time of the micro-fluid chip is shortened, the period is short, the consumption of a reagent is less and the batch production is convenient; the hard micro-fluid chip can be flexibly packaged and combined with various substrates; the packaging is independent of external high temperature and high pressure environments; and the packaging strength is high and the speed is high.
Owner:TSINGHUA UNIV +1
Preparation method of super-hydrophilic cell growth surface
The invention discloses a preparation method of a super-hydrophilic cell growth surface. The preparation method sequentially comprises the following steps: 1) preprocessing, to be specific, mixed gases of Ar and O2 are introduced into a plasma reaction chamber, a polystyrene substrate is subjected to plasma etching treatment; 2) an acrylic monomer is applied on the surface of the preprocessed polystyrene substrate in a spraying manner, so that the surface of the polystyrene substrate is grafted with the acrylic monomer, and the hydrophilic cell growth surface is formed. The super-hydrophilic cell growth surface has a lasting stable super-hydrophilic effect, and the super-hydrophilic effect is measured by a contact angle meter to be less than 10 degrees. Besides, the material physical property of a polystyrene plastic is not changed, the biological property for cell culture is met, and the high transparency of the polystyrene substrate is guaranteed.
Owner:GUANGZHOU JET BIOFILTRATION CO LTD
Serotype 8 recombinant adeno-associated virus preparation method
ActiveCN108179137AAddress process complexitySolve the process conditionsFermentationVector-based foreign material introductionSerum free mediaSerotype
The invention belongs to the field of bioengineering, and more specifically relates to a method for preparing serotype 8 recombinant adeno-associated virus, The method comprises the following steps: 1) using a serum-free medium for culturing 293T cell in a sheet carrier; 2) performing transient transfection on the cultured 293T cell by adeno-associated viral vector system plasmid to obtain the transfected cell; and the adeno-associated viral vector system plasmid comprises AAV expression vector plasmid, auxiliary plasmid pHelper and AAV capsid plasmid pRC8; and 3) continuously culturing the transfected cell, collecting and replacing the serum-free medium on time to obtain a medium containing the recombinant adeno-associated virus. The preparation method can guarantee production stability,preparation and purifying steps are saved, downstream virus purifying difficulty is obliviously reduced, and the preparation method is especially suitable for industrial scale-production of the serotype 8 recombinant adeno-associated virus.
Owner:BRAINVTA (WUHAN) CO LTD
Bioreactor for in vitro culture of neurotrophic factors
InactiveCN101665768APromote growthReduce protein consumptionTissue/virus culture apparatusNervous systemCulture plates
The invention discloses a bioreactor for in vitro culture of neurotrophic factors, which comprises an upper cover and a lower cover which are provided with concave cavities. The concave cavities of the upper cover and the lower cover are closed in opposite directions and fixed to form a box body, a culture plate through which cerebrospinal fluid can pass is arranged in the cavity of the box body,a polyether sulfone semi-permeable membrane is covered on the culture plate, the culture plate is embedded to the middle lower part of the concave cavity of the lower cover and positioned between theupper cover and the lower cover, both the upper part and the lower part of the culture plate are spaces in which the cerebrospinal fluid flows, the upper cover is provided with an input nozzle, the lower cover is provided with an output nozzle, and the upper end face of the upper cover is provided with a plurality of holes through which the air enters the box body. The bioreactor enables the cerebrospinal fluid of a nervous system dysfunction patient to be circulated in the bioreactor and fully mixed with the neurotrophic factors secreted by star-shaped gliocytes to form mixed cerebrospinal fluid containing the neurotrophic factors to be redelivered to the lower cavity of the arachnoid membrane or the ventricular system of the patient, thereby realizing promotion of recovery of functionaldeficiency or functional disorder of the central nervous system caused by diseases or injury, and remarkably improving the life quality of the patient.
Owner:THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
Primary culture method of embryonic cell tissue pieces of Chinese softshell turtle
The invention discloses a primary culture method of the embryonic cell tissue pieces of a Chinese softshell turtle. The primary culture method comprises the following steps of: (1) collecting the fertile eggs of the Chinese softshell turtle, and disinfecting; (2) breaking the eggshells of the fertile eggs, separating embryos from yolk, cleaning the separated embryonic tissues with a 1*PBS (Phosphate Buffer Solution), and then soaking for sterilization; (3) cleaning the embryonic tissues for 4-5 times in an M199 culture medium, then transferring into a complete culture medium, cutting the embryonic tissues into pieces to obtain tissue pieces, and then cleaning the tissue pieces once by using the complete culture medium; (4) uniformly dispersing the tissue pieces to the inner wall of a cell bottle by using a sterile disposable inoculating needle so that the tissue pieces grow for 3-4 hours in a way of being attached to the wall, then adding the complete culture medium to submerge the tissue pieces, and culturing at the constant temperature of 30-31 DEG C for more than 3 days. The primary culture method disclosed by the invention has the advantages of easy operation, convenient culture, no need of specific cell separation liquid and high culture success rate.
Owner:ZHEJIANG INST OF FRESH WATER FISHERIES
Low-serum protein-free culture medium applicable to Marc-145 cell growth and preparation method thereof
InactiveCN105176915AImprove stabilityReduce riskVertebrate cellsArtificial cell constructsChemical IngredientsAmino acid
The invention belongs to the field of cell culture and particularly relates to a low-serum protein-free culture medium applicable to Marc-145 cell growth and a preparation method thereof. The culture medium comprises amino acid, vitamin, inorganic salt, micro elements and other ingredients. The low-serum protein-free culture medium applicable to Marc-145 cell growth and the preparation method of the low-serum protein-free culture medium have the advantages of being free of protein and animal source ingredients and definite in chemical ingredient, the interference to cell culture of serum is industrially reduced, the production cost is lowered, and great convenience is brought to industrial production of cells.
Owner:南京三生生物技术股份有限公司
A serum-free medium for mesenchymal stem cells
ActiveCN106906182BPromotes adherent growthEasy to add componentsCulture processSkeletal/connective tissue cellsHydrocortisonePancreatic hormone
The invention discloses a serum-free medium for mesenchymal stem cells, which comprises a basal medium and supplementary components added to the basal medium, the supplementary components include L-glutamine, non-essential amino acids, L-ascorbic acid, Sodium selenate, fibronectin, ethanolamine, hydrocortisone, trypsin inhibitor, human transferrin, human insulin, bFGF, TGF‑β1 and PDGF‑BB, this medium overcomes the serum-free culture in the prior art The base has poor cell adhesion, relatively complex components, and does not support primary cell culture.
Owner:北京赛斯达生物技术有限公司
Plush-type bioreactor artificial liver system
ActiveCN106267397AGuaranteed adherent growthReduce adhesionBioreactor/fermenter combinationsBiological substance pretreatmentsHollow fibreArtificial liver
The invention discloses a plush-type bioreactor artificial liver system. The plush-type bioreactor artificial liver system comprises a plasma separating / recirculating part and a bioreactor circulating part which are mutually connected through a pipe, wherein the plasma separating / recirculating part comprises a blood transfusion port, a blood pump, an arterial ampulla, a plasma separator, a venous ampulla and a blood retransfusion port which are sequentially connected; a blood inlet, a plasma outlet and a blood cell outlet are formed in the plasma separator; the blood inlet is communicated with the outlet of the arterial ampulla; an artificial liver bioreactor comprises a reactor housing, and a plurality of pieces of plush and a plurality of hollow fiber tubes, which are arranged inside the inner chamber of the reactor housing; the plurality of pieces of plush and the plurality of hollow fiber tubes are arranged in parallel and are alternately spaced. The plush-type bioreactor artificial liver system has the beneficial effects as follows: the plush-type bioreactor artificial liver system significantly increases the adherent area of liver cells in the reactor, improves the uniformity of liver cell distribution, can maintain good cell activity, and benefits maximum achievement of functions of the liver cells, so that the best therapeutic effect is achieved.
Owner:WUHAN TOGO MEDITECH CO LTD
Culture method of mesenchymal stem cells contained in umbilical cord blood
InactiveCN113249315AAccelerated settlementPromote proliferationCell dissociation methodsCulture processHydroxyethyl starchAstragalus polysaccharide
The invention discloses a culture method of mesenchymal stem cells contained in umbilical cord blood, belongs to the technical field of stem cell culture, and solves the problems that the success rate of separation of umbilical cord blood mesenchymal stem cells is low and the cell proliferation rate is low in the culture process in the prior art. The culture method specifically includes the following steps: step S1, collecting and transporting umbilical cord blood; step S2, extracting the mesenchymal stem cells; step S3, performing primary culture; step S4, performing subculture; and step S5, performing cryopreservation. According to the culture method, firstly, effective umbilical cord blood is acquired, sedimentation of red blood cells is accelerated by adding macromolecular hydroxyethyl starch with the combination of a density gradient centrifugation method, the success rate of separation of a single mesenchymal stem cell is increased; a specially-made culture medium used for the primary culture contains astragalus polysaccharide, and the astragalus polysaccharide can also maintain the phenotype and characteristics of the umbilical cord blood mesenchymal stem cells while promoting cell proliferation; and the culture method provided by the invention is simple to operate, free of exogenous pollution, safe and reliable and high in cell proliferation rate.
Owner:上海南滨江细胞生物科技有限公司
Preparation method of collagen-based composite hemostatic powder
ActiveCN103721247BGood hemostatic effectGood biocompatibilityOrganic active ingredientsPeptide/protein ingredientsFreeze-dryingBiocompatibility Testing
The invention discloses collagen-based composite hemostasis powder and a preparation method thereof. The method is characterized by comprising the following steps: modifying type I collagen by using dencichine, preparing the modified type I collagen into a solution with the concentration of 1-2 percent by using polylysine-modified chitosan; preparing medical gelatin into a 1-3% solution; preparing the modified chitosan into a 1-3% solution; blending the three solutions according to a mass ratio of 0.5-1:2-8:8-2; stirring at the temperature of 4-10 DEG C for 8-10 hours, and performing freeze drying to prepare composite sponge; sequentially impregnating the composite sponge in a modifier and a blood coagulation factor in an ultrasonic cleaner, fully washing, performing freeze drying, and grinding by a grinder to prepare the collagen-based composite hemostasis powder. The powder has the effects of rapidly and effectively stopping bleeding, resisting bacteria, diminishing inflammation, relieving pain, promoting wound healing and repairing, has high adhesion property, hydrophilicity, biological degradability and biocompatibility and is low in price and suitable for wound hemostasis or repair.
Owner:BEIJING HOTWIRE MEDICAL TECH DEV CO LTD +1
Method for preparing dental pulp stem cells
InactiveCN109321522APromote growthShorten the timeCell dissociation methodsCulture processNeutral proteaseDigestion
The invention provides a method for preparing dental pulp stem cells. The method comprises the following steps of S1, collecting teeth; S2, separating the dental pulp stem cells, fixing the teeth, splitting the dental crown, fetching out dental pulp, putting the fetched dental pulp into PBS (phosphate buffer saline) for flushing, then preparing the fetched dental pulp into blocks with size of 1 to3 mm<3>, adding a mixed solution of 0.025% to 0.2% of I-type collagenase and 0.025% to 0.2% of neutral protease, digesting for 10 to 30 min at the temperature of 37 DEG C, adding the PBS, centrifuging at the speed of 1000 to 1500 rpm to remove supernatant, settling, adding a culture medium for moistening, attaching the tissue blocks into a cell culture dish, and putting into a CO2 (carbon dioxide) culture box to culture. By adopting the technical scheme, compared with the prior art, the method has the advantages that the enzyme used by the digestion wall-attaching method is a mixture of the I-type collagenase and the neutral protease, and the functions of the I-type collagenase and the neutral protease are mild, so that the restriction of surrounding tissues to the stem cells is reduced,and the damage to the cells is avoided; the purity of the separated stem cells is guaranteed, and the cell harvesting time is shortened.
Owner:山东康华生物医疗科技股份有限公司
Decidua parietalis mesenchymal stem cell culture medium for treating tumors and co-culture method
ActiveCN113322228AInhibition of proliferative abilityPromote growthCell dissociation methodsDead animal preservationMesenchymal stem cellStem cell culture
The invention discloses a decidua parietalis mesenchymal stem cell culture medium for treating tumors and a co-culture method. The decidua parietalis mesenchymal stem cell culture medium for treating tumors contains decidua parietalis mesenchymal stem cells and a selective culture medium, the decidua parietalis mesenchymal stem cells have a homing effect, a proliferation inhibiting effect and a cell cycle adjusting effect on various tumor cells.
Owner:GUANGDONG VITALIFE BIOTECHNOLOGY CO LTD
Permeable protection agent, serum-free frozen stock solution, and preparation and application methods of serum-free frozen stock solution
PendingCN109874780AReduced Possibility of ContaminationEasy to prepareDead animal preservationSerum freeObserved Survival
The invention relates to a permeable protection agent, a serum-free frozen stock solution, and preparation and application methods of the serum-free frozen stock solution. The permeable protection agent is fulvic acid. The technical scheme of the invention has the technical effects that the cost is low; the toxicity is low; and the survival rate of cells is high after resuscitation.
Owner:北京美迪阿姆科技发展有限公司
Rapid preparation of accellular porcine intestinal submucosa
ActiveCN108785746AImprove cleanlinessAvoid hard-to-remove risksTissue regenerationProsthesisAntigenCell-Extracellular Matrix
The invention provides a rapid preparation of accellular porcine intestinal submucosa. The method sequentially comprises the steps of decellularization treatment performed before degreasing treatment,the degreasing treatment, and secondary decellularization treatment and renaturation treatment which are performed after the degreasing treatment. The rapid preparation of the accellular porcine intestinal submucosa not only can quickly and effectively remove all relevant antigen components of all cells and the like in tissues, but also maintains the good chemical composition and mechanical properties of extracellular matrix (ECM).
Owner:承功(厦门)生物科技有限公司
Method for producing porcine pseudorabies gE gene deletion virus vaccine by using micro-carrier suspension culture cells
ActiveCN107198771APromotes adherent growthImprove surface propertiesViral antigen ingredientsArtificial cell constructsSuspension cultureCell Maintenance
The invention relates to a method for producing a porcine pseudorabies gE gene deletion virus vaccines by using a BHK cell line. The method comprises the following steps: resuscitating BHK21 cells and performing micro-carrier culture by adopting a low-serum culture medium; then replacing the low-serum culture medium with a cell maintenance solution, performing continuous perfusion culture, inoculating when the cell density is maintained at (1.5-3)*10<7> pcs / ml and continuously harvesting virus liquid for six days after 48 hours; concentrating, inactivating and sterilizing the harvested virus liquid to prepare the finished product vaccine. The method provided by the invention has the advantages that the vaccine is high in potency and stronger in mmunogenicity and can achieve a good immune effect without adding an immunoenhancer and promote the secretion of neutralizing antibodies in vivo after immunization; the immune protective rate achieves 100 percent, so that the vaccine efficacy evaluation criteria is fully reached.
Owner:广州渔跃生物技术有限公司 +1
Method for preparing feed layer cells by using R6-MEF carried with Xist Tale suppression transcription factor R6
ActiveCN108251377APromotes adherent growthGood cloneGenetically modified cellsSkeletal/connective tissue cellsMammalBiology
The invention discloses a method for preparing feed layer cells by using R6 transfected MEF. The method comprises the following steps: firstly using suppression transcription factor R6 transfected MEFbased on transcriptional activation effect material sample Tale combined inside an Xist first intron area to obtain immortal type MEF cell lines R6-MEF, after screening the various R6-MEF cell lines,selecting the R6-MEF cell lines with relatively high multipotential gene expression, and after mitomycin treatment, obtaining the novel feed layer cells R6-feeder, so that the obtained feed layer cells can be preferably used for maintaining the pluripotency iPSC of embryonic stem cells ESC and induced multipotential stem cells. According to the method, a new selection is provided for cultivationand research of stem cells, and a new technological and theoretical basis is provided for researching how to obtain and improve iPSCs of people, pigs, cows and other mammals.
Owner:INNER MONGOLIA UNIVERSITY +2
Modified film material for bioartificial liver
InactiveCN102911390APromotes adherent growthImprove hydrophilicitySuction devicesProsthesisFiberBioartificial liver device
The invention relates to a modified film material for a bioartificial liver. The modified film material is prepared by the following steps of: by taking a polyvinylidene fluoride hollow fiber film as a raw material, taking 2-methyl-2-acrylic acid-2-(2-methoxyethoxy) ethyl ester as a grafting monomer and taking benzophenone as an initiator, performing surface graft modification on the polyvinylidene fluoride hollow fiber film by employing an ultraviolet irradiation method. The modified film material has high hydrophilcity; compared with the unmodified film material in a miniature artificial liver reactor, the modified film material contributes to adherent growth of hepatic cells and maintenance of functional status and is suitable for serving as a film material of the bioartificial liver.
Owner:程向东
Four-in-one plush thread artificial liver bioreactor
ActiveCN105999448BSimple structureEasy to operateDialysis systemsMedical devicesOxygenatorsEngineering
The invention discloses a four-in-one plush thread type artificial liver bioreactor, which includes a constant temperature water tank and a three-in-one bioreactor placed inside it; the three-in-one bioreactor includes a cavity and is arranged in the cavity The first partition and the second partition in the body; the first partition and the second partition divide the cavity into three independent spaces from left to right, followed by plasma separator, oxygenator and reactor ; The constant temperature water tank includes a water tank shell and a hot water inlet and a hot water outlet located on the water tank shell. The beneficial effects of the present invention are: after the blood enters the plasma separator, the plasma enters the oxygenator and the reactor in turn, reducing unnecessary pipelines; it can provide a suitable metabolic environment for cells and ensure cell activity; it can increase the effective cell filling volume and ensure The cells are evenly distributed laterally.
Owner:WUHAN TOGO MEDITECH CO LTD
Preparation method of mesenchymal stem cells derived from early mesoderm
InactiveCN111518756AReasonable designImprove separation efficiencyCell dissociation methodsSkeletal/connective tissue cellsCell extractionMesenchymal stem cell
The invention discloses a preparation method of mesenchymal stem cells derived from early mesoderm. The method comprises the steps of cleaning, enzymolysis, filtering, primary culture, subculture, induced differentiation and induced differentiation. The preparation method has the beneficial effects that the separation efficiency of the stem cells is improved, so that temperature change during heating and refrigeration of the stem cells becomes more smooth, cell inactivation caused by the temperature change in a cell extraction process is avoided, the problem of the extraction rate of the cellsis reduced, the separation speed of the mesenchymal stem cells can be improved, purity of the mesenchymal stem cells in a separation process is ensured by polylysine, and growth of the cells adheringto walls after separation is promoted.
Owner:河南省遗传资源细胞库有限公司 +1
A kind of fabrication method of rigid microfluidic chip
ActiveCN107305214BHigh light transmittanceReduced autofluorescenceMaterial strength using tensile/compressive forcesTransmissivity measurementsMicron scaleEpoxy
The invention discloses a manufacturing method for a hard micro-fluid chip. The manufacturing method comprises the following steps: preparing an upper chip and chemically bonding and packaging the upper chip under a semi-cured state with a substrate, thereby acquiring the hard micro-fluid chip. The upper chip can be prepared from epoxy resin or amino resin; when the upper chip is prepared, the heating curing temperature is 45-85 DEG C and the time is 15 minutes to 8 hours; a thermal polymerized epoxy resin material is used after the two materials of prepolymer and curing agent are mixed, the viscosity thereof after mixing is low (approximate to mineral oil viscosity), the rolling printing for a tiny structure at micron scale and even nanometer scale is convenient and the rolling precision is high; the mixture can be solidified within 40min under the temperature of 80 DEG C, the preparation time of the micro-fluid chip is shortened, the period is short, the consumption of a reagent is less and the batch production is convenient; the hard micro-fluid chip can be flexibly packaged and combined with various substrates; the packaging is independent of external high temperature and high pressure environments; and the packaging strength is high and the speed is high.
Owner:TSINGHUA UNIV +1
Bioreactor for in vitro culture of neurotrophic factors
InactiveCN101665768BPromotes adherent growthImprove the quality of lifeTissue/virus culture apparatusSubarachnoid spaceLife quality
Owner:THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
Super-hydrophilic coating layer for cell culture and preparation method
PendingCN112480750AEasy to operateLow costCell culture supports/coatingHyaluronic acid coatingsBiologyPolyvinyl alcohol
The invention provides a super-hydrophilic coating layer for cell culture and a preparation method. According to the super-hydrophilic coating layer for cell culture and the preparation method, the polyvinyl alcohol-chitosan interpenetrating network super-hydrophilic coating layer is coated on a cell culture device in a spraying, dip-coating or roll-coating manner and the like, the super-hydrophilic coating layer has a durable and stable super-hydrophilic effect, and the water contact angle is smaller than 10 degrees; the prepared coating layer of the cell culture device is strong in adhesiveforce, high in transparency, resistant to ultraviolet and water soaking, good in biocompatibility, low in cytotoxicity and high in cell adherence rate.
Owner:武汉中科先进材料科技有限公司
Plush thread bioreactor artificial liver system
ActiveCN106267397BGuaranteed adherent growthReduce adhesionBioreactor/fermenter combinationsBiological substance pretreatmentsFiberHollow fibre
The invention discloses a plush thread type bioreactor artificial liver system, which includes a plasma separation / reinfusion circulation part and a bioreactor circulation part, and the two parts are connected to each other through pipelines; the plasma separation / reinfusion circulation part includes sequentially connected blood Input port, blood pump, arterial jug, plasma separator, venous jug and blood return port; plasma separator is equipped with blood inlet, plasma outlet and blood cell outlet; blood inlet is connected with the outlet of arterial jug; bioartificial liver reaction The reactor comprises a reactor shell and a plurality of plush wires and a plurality of hollow fiber tubes arranged in the inner cavity of the reactor shell; the plurality of plush threads and the plurality of hollow fiber tubes are arranged in parallel and alternately arranged at intervals. The beneficial effects of the present invention are: significantly increasing the area of the hepatocytes that can adhere to the wall in the reactor, improving the uniformity of the distribution of the hepatocytes, maintaining good cell activity, and being conducive to the maximum play of the function of the hepatocytes, thereby achieving the best good therapeutic effect.
Owner:WUHAN TOGO MEDITECH CO LTD
Chitosan polyacrylamide hydrogel base material and preparation method thereof
The invention discloses a chitosan polyacrylamide hydrogel base material and a preparation method thereof. The chitosan polyacrylamide hydrogel base material is composed of an acrylamide solution, a methylene diacrylamide solution, H2O, an ammonium persulfate solution, tetramethyl ethylenediamine and a chitosan solution. The preparation method of the chitosan polyacrylamide hydrogel base material comprises a step of mixing and stirring, a step of transferring to a glass clamping plate for reacting, and a step of immersion. The chitosan polyacrylamide hydrogel base material has a simple technology and allows hydrogels having different hardnesses to be obtained, and the obtained hydrogels have the advantages of non-toxicity, easy adherence growth of cells, reduction of the reaching complexity of the cytomechanics, benefiting for the researches on the mechanical properties of the cells, and reduction of the researching cost.
Owner:CHONGQING UNIV
Plush thread bioartificial liver reactor
ActiveCN106222086BGood biocompatibilityLess prone to allergic reactionsBioreactor/fermenter combinationsBiological substance pretreatmentsHollow fibreHigh cell
Owner:WUHAN TOGO MEDITECH CO LTD
Serum-free cryopreservation liquid, and preparation method and preparation device thereof
InactiveCN113455494AEasy to prepareImprove survival rateDead animal preservationPrimary cellGlutamine
The invention relates to the technical field of cell cryopreservation, and concretely relates to serum-free cell cryopreservation liquid, and a preparation method and a preparation device thereof. The cryopreservation liquid comprisesa DMEM / F-12 basic culture medium, Nutridoma, pyruvic acid, L-glutamine and 0.1-0.41 g / L of an intracellular and extracellular cryoprotectant. The cell cryopreservation liquid without serum or DMSO is determined in formula components and has very high biological safety and clinical application prospects, the recovery survival rate of cells cryopreserved with the cell cryopreservation liquid reaches 87% or above, good cell viability and physiological characteristics can be maintained, and the cryopreservation liquidis very suitable for the related research field of primary cells and stem cells.
Owner:南京生航生物技术有限公司
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