56results about How to "Reduced autofluorescence" patented technology

This invention provides an inexpensive and rapid method for fabricating a high-anisotropic-etch ratio, shaped glass structures using a novel photosensitive glass composition. Structures of the photosensitive glass may include micro-channels, micro-optics, microposts, or arrays of hollow micro-needles. Furthermore, such shaped glass structures can be used to form a negative mold for casting the shape in other materials.

This invention provides an inexpensive and rapid method for fabricating a high-anisotropic-etch ratio, shaped glass structures using a novel photosensitive glass composition. Structures of the photosensitive glass may include micro-channels, micro-optics, microposts, or arrays of hollow micro-needles. Furthermore, such shaped glass structures can be used to form a negative mold for casting the shape in other materials.

This invention provides an inexpensive and rapid method for fabricating a high-anisotropic-etch ratio, shaped glass structures using a novel photosensitive glass composition. Structures of the photosensitive glass may include micro-channels, micro-optics, microposts, or arrays of hollow micro-needles. Furthermore, such shaped glass structures can be used to form a negative mold for casting the shape in other materials.

This invention relates to methods for the detection of one or more mRNA transcripts in paraffin-embedded tissue by “mRNA liberation in fixed-treated tissue or ‘MLIFTT’”. This method includes treating the tissue with ammonia-ethanol and sodium borohydride combined with pressure cooking of the tissue. The chemical treatments reduce the tissue autofluorescence and the physical treatments overcome the interference created by the fixation-induced chemical bonds. The methods of the present invention can be utilized to identify a plurality of mRNA transcripts in a microarray format.

The invention provides a near infrared fluorescent dye with a tumor specificity targeting performance and applications thereof. The near infrared fluorescent dye is NIR dye PC-002, and can be automatically absorbed and accumulated by canine tumor cells; and the dye can be specifically accumulated in the tumor parts, and will not absorbed by the normal cells, so the dye can be used for tumor specificity target molecule imaging. The NIR specific combining between the near infrared fluorescent dye and the tumor cell is achieved by activating a HIF1[alpha] / OATPs signal molecule and oxygen deficiency. Heterogeneity of tumor cells is overcome, specific target molecule conjugate with common characters can be designed according to the characteristics of oxygen deficiency and OATP high expression of tumor cells, and through the observation on specific combining between the NIR dye and other tumor cells, the near infrared imaging technology can be applied to early warning and rapid diagnosis of more tumors.

The invention relates to tumor-specific heptamethine cyanine near-infrared fluorescent dye and application thereof; the near-infrared fluorescent dye is ICG-03, may be spontaneously absorbed and accumulated by various human tumor cells, may specifically gather at tumor parts other than normal cells, and accordingly may serve for accurately diagnosing tumors. Molecules described herein have better optical features than ICG, are suitable for photodynamic therapy and tumor imaging, and are good in stability. The heptamethine cyanine near-infrared fluorescent dye of the invention is suitable for the accurate diagnosis and treatment of tumors.

The present invention provides a fluorescent probe and application thereof. The probe which is of an electronic structure of a donor-Pi-receptor has twisted intramolecular charge transfer and aggregation-induced luminescence, and can be excited by two-photon. The probe can help overcome a self-absorption problem caused by large commercial lipid droplet fluorescent dye background and small Stokes'displacement and is high in biocompatibility, high in brightness, low in background and good in light stability. At the same time, a two-photon excitation process is used in the probe, autofluorescence can be lowered, a signal-to-noise ratio can be improved, and a three-dimensional resolution and light stability can be improved; the probe proposed in the invention can be applied to lipid droplet imaging of various cells and tissue sections.

The invention discloses a manufacturing method for a hard micro-fluid chip. The manufacturing method comprises the following steps: preparing an upper chip and chemically bonding and packaging the upper chip under a semi-cured state with a substrate, thereby acquiring the hard micro-fluid chip. The upper chip can be prepared from epoxy resin or amino resin; when the upper chip is prepared, the heating curing temperature is 45-85 DEG C and the time is 15 minutes to 8 hours; a thermal polymerized epoxy resin material is used after the two materials of prepolymer and curing agent are mixed, the viscosity thereof after mixing is low (approximate to mineral oil viscosity), the rolling printing for a tiny structure at micron scale and even nanometer scale is convenient and the rolling precision is high; the mixture can be solidified within 40min under the temperature of 80 DEG C, the preparation time of the micro-fluid chip is shortened, the period is short, the consumption of a reagent is less and the batch production is convenient; the hard micro-fluid chip can be flexibly packaged and combined with various substrates; the packaging is independent of external high temperature and high pressure environments; and the packaging strength is high and the speed is high.

The present invention relates to a method for amplifying and detecting nucleic acid sequences in a reaction cartridge comprising the following steps, (i) providing a sample comprising at least one nucleic acid molecule, (ii) in a first reaction chamber of the cartridge providing reagents for an amplification reaction, (iii) mixing the sample with the amplification reagents, (iv) amplifying the at least one nucleic acid in the first reaction chamber of the cartridge, (v) transferring at least parts of the amplification reaction into a second and third reaction chamber of the cartridge each comprising a probe set, wherein (a) each probe set consists of at least three probes, (b) each of the probes is specific for a nucleic acid sequence, (c) there are at least two probes in each set which carry an identical label, (d) each of the probes in a given probe set that carries an identical label has a melting temperature (Tm) which differs by more than 2° C. from the other probe in said probe set with the same label, (e) wherein the probes carrying the identical label differ in melting temperature (Tm) in a way that they are distinguishable by melting point, (f) performing a melting point analysis in order to determine which of the probes has specifically bound a nucleic acid.

This invention relates to methods for the detection of one or more mRNA transcripts in paraffin-embedded tissue by “mRNA liberation in fixed-treated tissue or ‘MLIFTT’”. This method includes treating the tissue with ammonia-ethanol and sodium borohydride combined with pressure cooking of the tissue. The chemical treatments reduce the tissue autofluorescence and the physical treatments overcome the interference created by the fixation-induced chemical bonds. The methods of the present invention can be utilized to identify a plurality of mRNA transcripts in a microarray format.

An apparatus for capturing an optical molecular image of one or more internal regions of interest of a small animal having a cranio-caudal axis. The apparatus may include a support member for the animal in an immobilized state; an endoscope for insertion into and withdrawal from the body of the animal to allow the endoscope to be positioned selectively proximate the internal regions of interest within the body; a light source connected to the endoscope to provide illumination within the body at the regions of interest; a mechanism for rotating the animal about its cranio-caudal axis to enable image capture from different angles; and a device for capturing images of the one or more internal regions of interest from the different angles, due to light emitted from the one or more internal regions of interest through an external surface of the body in response to illumination from the light source. A corresponding method is disclosed.

Disclosed are methods and assays for detection of low concentration analytes such as proteins in a sample, using beads. Specially coated beads allow for femtomolar sensitivity through strong near-infrared fluorescence enhancement on plasmonic beads having gold nanostructures in a coating. By selecting different bead sizes and labeling with different fluorophores of plasmonic beads for immobilization of different capture antibodies, multiplexed plasmonic beads can be used for simultaneous quantification of various markers down to 0.01 pg / mL sensitivity. Exemplified are human cytokine IL-6, IFN-gamma, IL-1 beta, VEGF and ovarian cancer biomarker CA-125. Using flow cytometry, a detection limit below that of glass bead based immunoassays by 2-3 orders of magnitude was achieved. The multiplexed plasmonic bead assay was used to simultaneously quantify cytokines and CA125 of ovarian cancer cell culture medium, demonstrating the potential of plasmonic bead based immunoassay for sensitive biological detection relevant to human diseases.

The present invention relates to a medical probe which consists of a cannula with a multilumen stylet inside. The multilumen contains at least two lumen. Both the multilumen as well as the cannula may have beveled ends. In the lumen straight optical fibers (i.e.no angle end face) are present that can be connected at the proximal end to a console. The cannula, multilumen, fiber system forming the medical probe comprises at least in one of the lumen of the multilumen more than one optical fiber. Preferably the source and detector fibers for the fluorescence detection are contained in one single lumen of the multilumen.

A standardized, buffered solution or “ink” composition for re-formulating or suspending biological membranes used in the fabrication of membrane arrays. The composition can enhance assay performance and prolong the shelf life of biological membrane arrays. The ink composition comprises a combination of at least two of the following six classes of reagents: 1) a pH buffer reagent; 2) a monovalent or divalent, inorganic salt; 3) a membrane stabilizer; 4) a solution viscosity control reagent; 5) a water-soluble protein; or, 6) a protease inhibitor. A method for fabricating a membrane array using the present ink composition is also described.

The present invention discloses a metal ion imprinting polymer preparation method, which comprises that a silane coupling agent, a cross-linking agent, a surfactant, a solvent and a metal ion template are used to prepare the metal ion imprinting polymer through a sol-gel method. The present invention further provides a metal ion imprinting polymer prepared through the method, and applications of the metal ion imprinting polymer. According to the present invention, the metal ion imprinting polymer can well meet the requirements on the metal-specific adsorption, and the adsorbents have characteristics of high temperature resistance, acid resistance, alkali resistance and short development cycle, and can selectively extract the trace target from the complex system.

The present invention provides a biochip cartridge wherein an elastic body is used for a substrate member in order to stabilize the feeding of blood or solution and whereby it is possible to avoid the risk of accidental contact of the operator with solutions due to mishandling.The biochip cartridge comprises a tabular substrate member formed using an elastic material and a flexible cover airtightly attached to the surface of the substrate member, wherein at least an area for storing biopolymers, an area for detecting desired biopolymers from the biopolymers that have been preprocessed, and a flow path for connecting the areas is formed on the substrate member, so that biopolymers can be successively moved from the biopolymer storage area to the biopolymer detection area through the flow path.

An optical device for fluorescence imaging equipped with a plurality of optical elements, wherein a silicone-based bonding agent containing a low-autofluorescent substance is interposed between the optical elements to bond the plurality of optical elements to each other.

The present invention relates to a method for amplifying and detecting nucleic acid sequences in a reaction cartridge comprising, (i) providing a sample comprising at least one nucleic acid molecule, (ii) in a first reaction chamber of the cartridge providing reagents for an amplification reaction, (iii) mixing the sample with the amplification reagents, (iv) amplifying the at least one nucleic acid in the first reaction chamber of the cartridge, (v) transferring at least parts of the amplification reaction into a second and third reaction chamber of the cartridge each comprising a probe set and performing a melting point analysis in order to determine which of the probes has specifically bound a nucleic acid.

The invention discloses a tissue autofluorescence quenching agent. The tissue autofluorescence quenching agent is prepared from a tissue autofluorescence quenching agent A and a tissue autofluorescence quenching agent B, wherein the tissue autofluorescence quenching agent A is a mixture of a phosphoric acid buffer solution with the pH value of 7.4 and sodium borohydride; and the tissue autofluorescence quenching agent B is a mixed solution of Sudan black B and absolute ethyl alcohol. An application method of the tissue autofluorescence quenching agent comprises the following steps: S1, dewaxing paraffin sections to water: sequentially putting the sections into xylene I for 15 minutes, xylene II for 15 minutes, absolute ethyl alcohol I for 5 minutes, absolute ethyl alcohol II for 5 minutes,85% alcohol for 5 minutes and 75% alcohol for 5 minutes, and washing with distilled water; and S2, repairing antigen: placing the tissue sections in a repair box full of an EDTA antigen repair buffersolution. According to the preparation and the application of the tissue autofluorescence quenching agent, the preparation process of the agent is simple and rapid, the raw materials are easy to obtain, the tissue autofluorescence can be effectively reduced, and the signal-to-noise ratio in immunofluorescence analysis is improved.

It is intended to obtain a photosensitive resin composition that is capable of forming a highly fine pattern with a high aspect ratio while attaining the high adhesion of the pattern to a substrate, having low autofluorescence, and being exceedingly suitable for producing a biochip that causes exceedingly low damage on cultured cells. The photosensitive resin composition for forming a biochip of the present invention contains: an epoxy compound (A1) of a particular structure having an oxycyclohexane skeleton having an epoxy group; an epoxy compound (A2) of a particular structure which is a polyvalent carboxylic acid derivative having an epoxidized cyclohexenyl group; a cationic photoinitiator (B); and a solvent (C).

The invention belongs to the biotechnical field, and concretely relates to a pretreatment method and a pretreatment liquid used for fluorescence in situ hybridization of FFPE tissue slices. The pretreatment liquid comprises a pretreatment liquid I and a pretreatment liquid II; the component of the pretreatment I is alkylphenol polyoxyethylene (TX-10) and / or octylphenoxypolyethoxyethanol; and components of the pretreatment liquid II comprise alkylphenol polyoxyethylene (TX-10) and / or octylphenoxypolyethoxyethanol, and also comprise ethylene diamine tetraacetic acid (EDTA) and / or a citric acid buffer. Paraffin is directly dissolved and emulsified in aqueous environment with the temperature being higher than the melting point of the paraffin through the pretreatment liquid I and the pretreatment liquid II by using the hydrophilic and lipophilic characteristics of an emulsifier in order to complete hydration and permeation, so steps are simplified, and time is saved. The pretreatment liquid II also can destroy formalin fixation induced cross-linking among proteins, so the FFPE tissues can be easily digested by proteases, and the autofluorescence and the background of the tissues are substantially reduced.

Provided is a method of manufacturing a plastic substrate with lower auto-fluorescence and better specificity. The method includes: (a) preparing a plastic substrate having an Atomic Force Microscopic (AFM) surface roughness of Ra<3 nm or Rq<4 nm under the condition of 50 μm×50 μm or less; (b) treating the plastic substrate with plasma; and (c) treating the plastic substrate with a surface-modifying monomer. A plastic substrate manufactured by the method is also provided. The plastic substrate exhibits a remarkably low auto-fluorescence and thus better specificity for detection of target biomolecules, which enables the broad application of a plastic substrate, which can be easily designed to include a microfluidic structure relative to a glass substrate but has been limitedly used due to high auto-fluorescence, to microarrays, biochips, or well plates. Furthermore, the method of manufacturing the plastic substrate enables an enhancement in the surface specificity of the plastic substrate and easy adjustment of the surface characteristics of the plastic substrate.

Provided herein are methods, compounds, mixtures and formulations of antigen retrieval agent useful in retrieving antigens and improving the detection of amino acids, peptides and proteins or epitopes thereon in a tissue fixed with aldehyde-based cross-linking agents. Contacting the fixed tissue with a solution of the aldehyde scavenging agent causes reactivity with the aldehyde moieties to retrieve antigens and improve detection of the amino acids, peptides and proteins or epitopes. Also provided are kits comprising the antigen retrieval agent and, optionally, components for staining or detecting the proteins or the antigens or epitopes and instructions for using the kit. Further provided is a method for identifying an antigen retrieval agent. A fixed protein is contacted with an agent to be tested and heated in solution therewith. Detection of protein peaks via mass spectrometry indicates the tested agent is an antigen retrieval agent.

The invention discloses a method for manufacturing slices of plant leaves. Aqueous solution with a nonionic surfactant tween-20 is added in slice manufacturing procedures. The volume concentration of the tween-20 in the aqueous solution with the nonionic surfactant tween-20 is lower than 0.1%, and the usage of the aqueous solution used for manufacturing the slices ranges from 50 micro-l to 100 micro-l. The method has the advantages that the aqueous solution with the nonionic surfactants--tween-20 is added, so that the activity of materials can be kept, and dehydration and shrinkage of the materials can be reduced to the greatest extent; the effective observation time of the compressed slices made from fluorescent bacteria with markers can reach 18 h when the materials are about to be observed by laser confocal microscopes or other microscopes, fluorescence can be kept for 2 days, and observed images are almost free of auto-fluorescence of the plant leaves; only the aqueous solution with the tween-20 needs to be added when the slices are manufactured by the aid of the method for manufacturing the slices of the plant leaves, and accordingly the method is particularly suitable for manufacturing slices of citrus leaves and is easy to implement.

The invention discloses a method for improving the near-infrared emission intensity of an up-conversion material. Ga<3+> is doped in the process of synthesizing the NaGdF4: 18%Yb and 0.5% Tm up-conversion material by adopting a solvothermal method, the NaGdF4: 18%Yb, 0.5%Tm and x%Ga<3+> rare earth up-conversion luminescent material is prepared, and the value of x is 0.5-5. The particle size of thematerial is 9-11 nm, the particle size is small, the morphology and size distribution is uniform, and the material can be used as a multi-mode biological imaging contrast agent for UCL imaging, MRI imaging and CT imaging. When Ga<3+> is doped into a matrix, fluorescence emission of Tm<3+> in a luminescence center at the near-infrared wavelength of 800 nm is remarkably enhanced, near-infrared light emission at the near-infrared wavelength of 800 nm has good penetrability to biological tissue, light damage to the biological tissue is small, and fluorescence imaging application of the material in a living body is facilitated. The development of the multimode imaging material has a potential application prospect in the field of biomedicine.

The invention provides a near-infrared fluorescent probe taking benzylboronic acid pinacol ester as a detection group as well as a preparation method and application of the near-infrared fluorescent probe, aiming at overcoming the defects that most of existing nitrosyl peroxide anion detection probes are low in sensitivity, high in detection limit, relatively poor in water solubility and relatively short in fluorescence emission wavelength. The near-infrared fluorescent probe designed by the invention is relatively low in light scattering level, autofluorescence of biological cells can be reduced, larger tissue penetration can be realized, and rapid, sensitive, efficient and specific detection of the nitrosyl peroxide anions can be realized through a fluorescence emission spectrum.