82 results about "Antigen retrieval" patented technology

A method is disclosed for reversing fixation-induced cross-linking in tissue specimens that have been preserved for histological examination. The method involves placing the fixed tissue in a liquid under elevated temperature and pressure conditions that are sufficient to reverse the fixation-induced cross-linking, restore antigenicity to proteins, and permit improved molecular and proteomic analysis of the preserved tissue specimen. Methods are also disclosed for processing tissues for histological examination under elevated pressure conditions that enhance the perfusion of liquid reagents into the tissue and reduce overall processing times.

The present invention concerns a method and apparatus for automatic staining or other processing of a biological sample on a slide, perhaps robotically, by applying predetermined amounts of reagents in a predetermined sequence according to a processing protocol, the processing including pre-treatment steps, under the control of an adaptive processing control system. Further provided is a method of antigen retrieval, the method comprising contacting, within a heated processing tank of an automated staining apparatus, at least one biological sample with at least one composition for antigen retrieval comprising at least one chelating agent, at least one detergent and at least 50% by volume of at least one liquid that facilitates antigen retrieval.

The present invention relates to the field of cancer. More specifically, the present invention provides methods and compositions useful for diagnosing or predicting cancer in a patient. In one embodiment, a method for identifying a patient as having cancer comprises the steps of (a) providing a formalin-fixed, paraffin-embedded or fresh frozen sample of patient tissue; (b) steaming the sample in antigen retrieval buffer; (c) incubating the sample in hydrochloric acid (HCl); (d) incubating the sample with an affinity reagent specific for 5hmC under conditions to form a complex between the affinity reagent and 5-hydroxymethylcytosine (5hmC) present in the sample; (e) detecting the complexes formed between 5hmC and the affinity reagent with secondary detection reagents; (f) quantifying 5hmC levels; and (g) identifying the patient as having cancer if the 5hmC levels in the sample are reduced as compared to a control.

Provided herein are methods, compounds, mixtures and formulations of antigen retrieval agent useful in retrieving antigens and improving the detection of amino acids, peptides and proteins or epitopes thereon in a tissue fixed with aldehyde-based cross-linking agents. Contacting the fixed tissue with a solution of the aldehyde scavenging agent causes reactivity with the aldehyde moieties to retrieve antigens and improve detection of the amino acids, peptides and proteins or epitopes. Also provided are kits comprising the antigen retrieval agent and, optionally, components for staining or detecting the proteins or the antigens or epitopes and instructions for using the kit. Further provided is a method for identifying an antigen retrieval agent. A fixed protein is contacted with an agent to be tested and heated in solution therewith. Detection of protein peaks via mass spectrometry indicates the tested agent is an antigen retrieval agent.

The invention discloses an antigen pre-processing method for preparing an antibody, and relating to the technical field of immunology. Purified antigen protein is processed by adopting an antigen retrieval solution in the pre-processing method. Aiming at the characteristics of an immunohistochemistry technology and disadvantages in the prior art, the invention aims to provide the antigen pre-processing method; and the antigen pre-processing method has the advantages of being simple and convenient to operate, low in production cost and the like. The key step of immunohistochemistry in the invention is antigen retrieval; the antigen is processed by selecting a corresponding antigen retrieval solution; the antigen pre-processing method, which can be applied to immunohistochemistry, is designed in a targeted manner, i.e., the immunohistochemistry retrieval manner of the antibody is selected according to the processing manner of the antigen; the antigen pre-processing method is simple and convenient to operate and low in production cost; and a novel effective way is provided for preparing the antibody applied to immunohistochemistry.

The present invention relates to an in-vitro immunoassay method for detecting the presence of liver cancer cells in a subject. In the method of the present invention, antigen retrieval treatment based on heat-induced epitope retrieval method and antigen retrieval treatment based on protease-induced epitope retrieval method can be combined in the detection of glypican 3 antigen expression in liver cancer tissues to thereby detect the difference in the expression level or expression pattern of the glypican 3 antigen by immunohistochemical staining method. This enables samples, which has been determined by the conventional HIER method as highly expressing glypican 3, to be graded according to the expression level of glypican 3.

Disclosed are a pretreatment solution for immunohistochemical staining, which elutes a paraffin-containing embedding medium from a glass slide with a tissue specimen embedded in the medium, and retrieves antigenicity of the tissue specimen, and which is usable three or more times, and a pretreatment solution concentrate for immunohistochemical staining which allows ready preparation of the pretreatment solution and a method of immunohistochemical staining using the same. The pretreatment solution contains an antigen retrieving agent, particular nonionic surfactants, and cyclodextrin or a derivative thereof, with the balance being not less than 80 mass % of water. The content of the antigen retrieval agent is such that the pH of the pretreatment solution is in a predetermined range, and the content of cyclodextrin or a derivative thereof is a particular amount.

Compositions and methods for preparing a sample for immunological staining are provided. Compositions include kits comprising a first solution comprising a surfactant and a second solution comprising a chaotropic agent. Methods comprise contacting a sample, such as cells or tissues, with a first solution comprising a surfactant and then contacting the sample with a second solution comprising a chaotropic agent. The method does not require extreme heat for antigen retrieval and therefore, maintains the cellular morphology of the sample.

Provided in the present invention are an immunohistochemical quality control reference object and a method for quality control using the reference object. In the present invention, particles solidified by a glucan solution are used to simulate biological tissue, and target antigen proteins are uniformly embedded in the particles. The solid glucan particles in which the target antigens are embedded are formed into a paraffin block after being dehydrated, transparentized, wax-dipped and paraffin-embedded, and the paraffin block is stuck to a glass slide after being sliced on a slicer, so as to be dyed together with tissue to be detected. In the method of the present invention, negative control, positive control and antigen retrieval control are designed according to different materials, and the purpose of quality control is achieved through a dyeing result of a reference object.