Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

EDTA antigen retrieval liquid

A repair solution and antigen technology, applied in the field of immunohistochemistry, can solve the problems of antigen destruction, volatile, drying of tissue slices, etc., and achieve the effect of good repair effect and non-volatile effect.

Active Publication Date: 2016-10-26
安徽世纪康大生物科技有限公司
View PDF8 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The solvent of the above-mentioned antigen retrieval solution is water. If it is applied to an automatic immunohistochemical staining instrument, on the one hand, the antigen retrieval solution needs to be used in a large amount to achieve a good repair effect, and the tissue section cannot be completely cut with a small amount. On the other hand, it has a low boiling point and is easy to volatilize at a higher temperature, which can easily lead to drying of tissue sections, and once drying occurs, the antigens in tissue sections will become irreversible. Reversed damage beyond repair

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] An EDTA antigen retrieval solution, each 1000mL of EDTA antigen retrieval solution includes: Tris-Base 1.2114g, EDTA 0.37224g, Tween-20 500μl, triethanolamine 150mL and the remainder of single distilled water.

[0028] The preparation method is as follows: Weigh Tris-Base and EDTA according to the ratio, dissolve Tris-Base and EDTA with single-distilled water, add the formula amount of triethanolamine, add Tween-20 after complete dissolution, and finally dilute with single-distilled water to Rated Capacity.

Embodiment 2

[0030] An EDTA antigen retrieval solution, each 1000mL of EDTA antigen retrieval solution includes: Tris-Base 1.2114g, EDTA 0.37224g, Tween-20 500 microliters, ethylene glycol 325mL and the remainder of single distilled water.

[0031] The preparation method is as follows: Weigh Tris-Base and EDTA according to the ratio, dissolve Tris-Base and EDTA with single-distilled water, add the formula amount of ethylene glycol, add Tween-20 after complete dissolution, and finally dilute the volume with single-distilled water To rated capacity.

Embodiment 3

[0033] An EDTA antigen retrieval solution, each 1000mL of EDTA antigen retrieval solution includes: 1.20g Tris-Base, 0.35g EDTA, 400 microliters of Tween-20, 50mL triethanolamine and the remainder of monodistilled water.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of immunohistochemistry and discloses EDTA antigen retrieval liquid. Each 1,000 mL of EDTA antigen retrieval liquid is prepared from 1.20-1.25 g of Tris-Base, 0.35-0.40 g of EDTA, 400-600 microliters of twain-20, 50-500 mL of triethanolamine or ethanediol or triethanolamine and the balance single steaming water. The EDTA antigen retrieval liquid can achieve infiltration, is good in retrieval effect and can retrieve tissue slices with a little amount, the retrieved tissue slices can be dyed easily, and the dyeing effect is good. The retrieval liquid is not prone to volatilization and can be used in cooperation with a full-automatic immunohistochemical instrument.

Description

Technical field [0001] The invention relates to the technical field of immunohistochemistry, in particular to an EDTA antigen retrieval solution. Background technique [0002] Immunohistochemistry technology is a detection technology that has developed rapidly in pathological diagnosis in recent years. As an important pathological auxiliary diagnosis method, it has received increasing attention in clinical applications. With the application and promotion of automatic immunohistochemical staining apparatus, the requirements for staining quality are also increasing. Among them, antigen retrieval plays an extremely important role in the entire immunohistochemical staining process, and the quality of the repair conditions directly determines the quality of the final staining result. Because of its own structural characteristics, the automatic staining instrument has a small volume for holding the repair solution, and it cannot completely immerse the tissue section in the repair solu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/30
CPCG01N1/28G01N1/30
Inventor 李思勇余向东
Owner 安徽世纪康大生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com