The invention discloses an application of a
Glypican-1
protein in the diagnosis of
pancreatic cancer, a detection method of a
Glypican-1 positive
exosome concentration, and a use of the detection method. A nano-
plasma enhanced scattering (nPES) detection technology can be used to quantify exosomes in serum, an
exosome extracting process is omitted, the characteristic
antigen of the exosomes is used as a target, and the
antigen and a corresponding
antibody carrying gold nanoparticles form an
antigen-
antibody complex in order to obtain exosomes, and the amount of the exosomes is reflected by using the light
radiation principle of the gold nanoparticles. The
Glypican-1 is a
pancreatic cancer-derived
exosome biomarker, and the content of Glypican-1 positive exosomes in
blood plasma has specific specificity even in early
pancreatic cancer, so the exosome content index can be used to diagnose the pancreatic
cancer, and the exosome level is closely related to the staging and progression of pancreatic
cancer patients. Experiments prove that the method using the nPES detection technology to detect the content of the Glypican-1 exosomes in the serum is concise and accurate, and has better sensitivity and specificity than CA19-9 in the diagnosis of the pancreatic
cancer.