The invention discloses a three-dimensional macroporous graphene, carbon nano tube and molybdenum disulfide composite material, and a preparation method and an application of the composite material, and relates to a nano composite material, and a preparation method and an application of the nano composite material. The composite material, the preparation method and the application aim at solving the problems of poor stability and too low first cycle efficiency of existing molybdenum disulfide serving as a cathode active substance of a lithium ion battery. The method comprises the steps of 1, preparing a three-dimensional macroporous graphene / foam metal composite, 2, preparing a three-dimensional macroporous graphene / carbon nano tube / foam metal composite, 3, preparing a three-dimensional macroporous graphene / carbon nano tube, 4, loading molybdenum disulfide by a hydrothermal method, and 5, annealing the three-dimensional macroporous graphene / carbon nano tube loaded with molybdenum disulfide to obtain the three-dimensional macroporous graphene, carbon nano tube and molybdenum disulfide composite material.