The invention belongs to the technical field of
molecular biology, and particularly discloses a
reagent and a kit for rapidly extracting
nucleic acid from an FFPE sample and application of the
reagent and the kit. The combination
buffer solution in the
reagent is simple in formula and low in cost, the combination effect of the
nucleic acid and the magnetic beads can be remarkably improved, and the combination amount of the
nucleic acid is increased. According to the method, the
lysis buffer solution, the binding
buffer solution and the washing buffer solution with specific components are combined for use, tissue cells are fully lysed,
protein bound with nucleic acid is digested, the nucleic acid is completely released, then the released nucleic acid is specifically bound to the magnetic beads, the binding amount of the nucleic acid is increased, effective separation of the nucleic acid and other lysates is achieved, the magnetic beads combined with the nucleic acid are washed for multiple times, impurities such as
protein and lipid are thoroughly removed, and the nucleic acid isfinally eluted from the magnetic beads to obtain high-purity and high-yield nucleic acid. The reagent / kit is simple in composition, low in cost and convenient for nucleic acid extraction operation, and the extraction time can be greatly shortened.