The invention discloses a gold /
platinum bimetal nanocluster
fluorescence probe based on polyethyleneimine protection, and an application of the
fluorescence probe in aureomycin detection, and belongs to the technical field of
fluorescence probes. A
bimetal gold /
platinum nanocluster has a great
Stokes shift (about 150nm), can stably exist in a solution with extreme pH (potential of
hydrogen) and high
ion concentration, and releases stable fluorescence under long-time
ultraviolet irradiation, so that a wide application of the nanocluster in the fields of medicines and
biology is facilitated. In addition, the probe is used for detecting
tetracycline antibiotics based on a mechanism of fluorescence
quenching, and aureomycin can be distinguished from
tetracycline by adding Al<3+> based on a mechanism of fluorescence enhancement, so that the purpose of specific aureomycin detection is achieved. The probe is used for detecting the aureomycin; a
linear range is 0.5-30 micrometers; and a
detection limit is 0.5 micrometers. In addition, real
object detection is successfully performed on the aureomycin in milk by the fluorescence probe, and a good effect is obtained, so that the fluorescence probe can be used for detecting aureomycin residues in food.