High-sensitivity-ratio fluorescent probe based on composite system of cadmium telluride quantum dots and europium ions and preparation method and application of high-sensitivity-ratio fluorescent probe
A technology of cadmium telluride quantum dots and ratio fluorescent probes, applied in the fields of chemistry and nanomaterials science, can solve problems such as non-reproducibility, and achieve the effects of good selectivity, simple raw materials, and good anti-interference.
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Embodiment 1
[0039] 1. Preparation of cadmium telluride quantum dot solution
[0040] Combine 0.0319g tellurium powder and 0.05g NaBH 4 Mix it in 2mL ultrapure water, and continue to introduce nitrogen to maintain an oxygen-free environment. The solution was stirred in an ice bath and reacted for 10 hours. The mixed solution gradually changed from black to white, and NaHTe was formed in the upper layer. Dissolve 0.2284g of cadmium chloride and 220μL of mercaptopropionic acid in 120mL of ultrapure water, adjust the pH to 9 with 1M NaOH solution, then bubbling with nitrogen for 30 minutes to remove oxygen in the solution. Dilute sulfuric acid was injected into NaHTe solution under anaerobic environment, and bubbling continuously to make the generated H 2 Te is transferred to the cadmium chloride solution to generate the precursor of CdTe quantum dots. Heat to 105°C and reflux for 30 minutes, remove the heat source, and cool to room temperature. The prepared cadmium telluride quantum dot aque...
Embodiment 2
[0049] 1. Preparation of cadmium telluride quantum dot solution
[0050] The preparation process of this step is the same as in Example 1.
[0051] 2. Preparation of Europium ion solution
[0052] The preparation process of this step is the same as in Example 1.
[0053] 3. Preparation of tetracycline ultrapure water test solution
[0054] The preparation process of this step is the same as in Example 1.
[0055] 4. Preparation of dual emission ratio fluorescent probe
[0056] Add 200 μL of cadmium telluride quantum dot solution, 32 μL of 10 mM europium nitrate solution, 200 μL of 100 mM Tris-HCl buffer solution at pH 8, tetracycline solution and ultrapure water to the cuvette, and the final total volume is 2mL mixed test system (the concentration of tetracycline in this test system varies from 0 to 80 μM), mix well and then stand and incubate for 5 min to make it fully react.
[0057] 5. Drawing of calibration curve for quantitative test
[0058] Use excitation light with a wavelength of ...
Embodiment 3
[0062] 1. Preparation of cadmium telluride quantum dot solution
[0063] The preparation process of this step is the same as in Example 1.
[0064] 2. Preparation of Europium ion solution
[0065] The preparation process of this step is the same as in Example 1.
[0066] 3. Preparation of interference substance to be tested
[0067] Preferably, the inorganic ions (K + , Ca 2+ , Na + , Mg 2+ , Fe 2+ ,Zn 2+ , SO 4 2- , Cl - , CO 3 2- ), amino acids (Gly, His, Lys, Arg, Glu, Cys), antibiotics (chlortetracycline, oxytetracycline, doxycycline, amoxicillin, carbenicillin sodium, gentamicin, kanamycin , Ciprofloxacin, chloramphenicol, sparfloxacin), glutathione, ascorbic acid, glucose and other interference substances to be tested, the concentration is 1M.
[0068] 4. Preparation of dual emission ratio fluorescent probe
[0069] Add 200 μL of cadmium telluride quantum dot solution, 32 μL of 10 mM europium nitrate solution, 200 μL of 100 mM Tris-HCl buffer solution at pH 8, and ultrapure water t...
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