The invention provides a clamping and positing device capable of automatically aligning center and a using method thereof, relating to a tooling clamp capable of automatically aligning center and being used in a plurality of fields of mechanical manufacture, printing, surface coating, electronic assembly and the like. The device comprises a driving mechanism, a clamping mechanism, a guiding mechanism and a supporting mechanism, wherein, the driving mechanism comprises a driving device and a driving force transmission device; the clamping mechanism comprises a positioning board (5); the supporting mechanism comprises a base (7) and a fixed seat (10); the driving mechanism, the clamping mechanism and the guiding mechanism are supported by the supporting mechanism; the driving mechanism drives the positioning board (5); the guiding mechanism ensures that the positioning board (5) moves according to a guided movement route; thus, the positioning board (5) can clamp a piece (13) to be processed, and the central reference position of the piece (13) to be processed is automatically positioned on the station for positioning a centre (central line or central point). The device can automatically align center and eliminate central line / point position offset.