37 results about "Mouse Nerve" patented technology

The invention relates to a medicament for nerve regeneration promotion, and particularly to a nerve regeneration promotion injection and a preparation method thereof. The nerve regeneration promotion injection is composed of the following ingredients in proportion: 280-320 micrograms of mouse nerve growth factor for injection, 19-21ml of single sialic acid four hexose ganglioside sodium injecta, 18-22ml of cerebroside-kinin injecta, 9-11ml of mecobalamine injecta and 9-11ml of dexamethasone acetate injecta. The five effective ingredients of the medicament provided by the invention are combined together and cooperative actions of multiple nutritive factors are utilized, so that the medicament has a significant curative effect on repairing of injured nerve, especially the repairing of spinal nerve injury. The medicament provided by the invention also has a protection effect on spinal cord transected injury at the early stage.

The invention discloses a method for preparing a mouse nerve growth factor (NGF) and a method for preparing a mouse nerve growth factor for injection. The method for preparing the mouse nerve growth factor adopts 100KD and 5KD of ultrafiltration membrane filtration and pasteurization processes on the basis of the conventional process, can effectively filter a macromolecular virus, can further inactivate potential viruses, such as pseudo rabies viruses (PRV), Sindbis viruses, encephalomyo-carditis (EMC) viruses and the like by pasteurization at the same time, does not introduce any pollutant, such as a foreign organic solvent, a detergent and the like, and can keep high purity, high specific activity and high safety of the mouse nerve growth factor.

The invention discloses a double-antibody sandwich enzyme immune gauging method of mouse nerve growth factor, which detects whether the NGF exists and the content qualitatively or quantatively.

The invention discloses a preparation method of a mouse meningeal single-cell suspension. The preparation method comprises the following steps of: 1) preparing a reagent and a culture medium; 2) carrying out tissue pretreatment; 3) carrying out tissue digestion and dissociation; 4) terminating digestion; 5) filtering by a micron cell filter; 6) obtaining a target tissue single-cell suspension; and 7) measuring the yield of living cells. The preparation method of the mouse meningeal single-cell suspension can be used for quickly and efficiently obtaining the mouse meningeal single-cell suspension at low cost and ensuring that dissociated cells keep good activity, can be applied to dissociation, separation and purification of mouse meningeal cells in a large range and establishment of a cell line, and provides important reference data for research of mouse nerve tissue single cells, gene functions and genetic breeding.

The invention belongs to the field of medication and particularly relates to an eye drop for treatment of nearsightedness and a preparation method of the eye drop. The eye drop comprises, by weight, 5-15 parts of silkworm larva, 5-15 parts of caulis dendrobii powder, 70-79 parts of fructus ligustri lucidi, 90-100 parts of semen cassiae, 50-80 parts of Vitamin B1, 50-70 parts of Vitamin C, 5-15 parts of borneol, 5-15 parts of mouse nerve growth factors, 50-80 parts of Vitamin B12, 5-15 parts of sodium chloride and 60-80 parts of piracetam. By the arrangement, problems that drug therapy is proneto side effects, defects prone to recurrence, risks and sequelae of surgical treatment and wearing optical glasses can only overcome the shortcoming of daily vision of patients with visual impairmentbut not the root cause are solved, and the eye drop adopts natural raw materials is developed into a safe, efficient and high-quality product with reasonable compatibility and synergistic effect, remarkable effect in the treatment of nearsightedness and safety and efficiency.

The inventions discloses a novel mouse nerve growth factor intravitreal injection drug delivery system and an application thereof, and relates to the field of mouse nerve growth factor-containing drug for treating oculopathy through intravitreal injection drug delivery. In allusion to the problems that non-ideal treatment effect and big side effects existing in a conventional drug delivery mode of a mouse nerve growth factor for treating oculopathy, the invention aims to provide the mouse nerve growth factor intravitreal injection drug delivery system which is characterized by comprising the mouse nerve growth factor and an ultrasonic contrast agent. The invention also provides the application of the mouse nerve growth factor intravitreal injection drug delivery system in preparing a drug for treating glaucomatous optic nerve damage, wherein the drug is used for being administrated by intravitreal injection under ultrasonic irradiation. The novel drug delivery mode provided by the invention has the advantages of good treatment effect, safety and reliability, and can be used for delaying or preventing occurrence and development of the glaucomatous optic nerve damage.

The invention discloses a method for extracting a nerve growth factor, wherein the method includes the steps: acidizing and centrifuging a supernatant containing the nerve growth factor to obtain an acidolysis supernatant, carrying out cation-exchange chromatography on the acidolysis supernatant, and collecting a target elution peak containing the nerve growth factor, to obtain a protein liquid 1;and carrying out hydrophobic chromatography on the protein liquid 1, and collecting a target elution peak containing the nerve growth factor, to obtain a target protein liquid. The method does not include the step of cationic reverse adsorption chromatography. Compared with the prior art, the method has simple steps and is safer and more reliable, and the purity, yield and sample activity of theobtained nerve growth factor sample are improved. The method not only is suitable for extracting the mouse nerve growth factor, but also is suitable for extracting the human nerve growth factor, and provides a new way for large-scale separation and purification of the nerve growth factor.

The present invention discloses cell adhesion molecule 5 (ICAM5) monoclonal antibody, a preparation method and applications thereof, wherein a preservation number of the hybridoma cell strain is CGMCC No.5798. The present invention provides the ICAM5 monoclonal antibody, which can be provided for ICAM5 antigen-based immunofluorescence and Western blotting, and other scientific researches, wherein test results show that the high and stable expression exists on the surface of human and mouse nerve cells. In addition, the antibody has characteristics of strong specificity and high titer, wherein an ELISA detection titer can be 1:400000, such that an ICAM5 antibody use concentration in a test is low, and an antibody use amount is low; and the ICAM5 antigen is positioned on the surface of nerve cell bodies and nerve fibers, such that structures of nerve cell bodies and nerve fibers can be clearly displayed with immunofluorescence staining.

The invention relates to a new application of caesalpinia minax, namely a new application of caesalpinia minax in preparation of medicines for treating an alzheimer's disease, in particular to a new application of a caesalpinia minax extract in preparation of medicines for treating the alzheimer's disease. The learning ability and the memory of dementia mice treated by the caesalpinia minax extract are obviously improved; the clearance rate of oxygen radicals in the brain tissue is improved; and beta-starch amyloid protein is reduced. The caesalpinia minax has a protecting effect on nerve injuries of AD mice and has positive reference for development and research and clinical treatment of medicines for clinical treatment of elder diseases of the alzheimer's disease and the like.

A mouse nerve retrograde tracing mark component comprises a cylinder tank body and an upper cover. A shape of the cylinder tank body is a cylindrical shape, a cross section of the cylinder tank body is a major arc, an upper cover is a curved plate and the cross section is an inferior arc, the upper cover fastens the cylinder tank body, and the upper cover and the cylinder tank body form a completecylindrical shape. Single and multiple retrograde tracing marking of mouse nerves can be realized so that direct observation of a mutual relation between different sources of motor neuron pools or neuronal subgroups in a spinal cord becomes possible.