Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for intrathecally injecting nerve regeneration promoting injection

A technique of nerve regeneration and intrathecal injection, applied in the field of medicine, can solve the problems such as effective therapeutic concentration and large molecular weight in parts without obvious and incapable spinal cord injury, and achieves reduction of secondary injury, good biological safety, and avoidance of molecular weight. big effect

Inactive Publication Date: 2015-05-06
赵廷宝
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Clinically, in addition to surgery for early spinal cord injury, the current drug treatment is still based on nerve growth factor and ganglioside, but intramuscular injection and intravenous administration have no obvious therapeutic effect, the reason may be that this type of molecular weight is large, It is not easy to pass through the blood-brain barrier, and cannot reach effective therapeutic concentrations at the site of spinal cord injury

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for intrathecally injecting nerve regeneration promoting injection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1 acute toxicity test

[0018] 40 healthy adult Wistar rats (SPF grade, provided by the Experimental Animal Center of the Academy of Military Medical Sciences, license number: SCXK-jun-2007-004), male or female, weighing 210-270 g, were randomly divided into 5 groups (L1 ~L5), 8 rats in each group, after successful anesthesia by intraperitoneal injection of 1% pentobarbital sodium (40mg / kg), the N6 compound was prepared immediately, respectively according to the first group 41.5μL / kg, the second group 83μL / kg, Inject 166 μL / kg in the third group, 332 μL / kg in the fourth group, and 664 μL / kg in the fifth group into the subarachnoid space, tail vein, muscle, and abdominal cavity of rats at L2 and 3 levels, 2 rats for each part, and perform acute treatment. For the toxicity test, observe for 3 days, and record whether the experimental animals have adverse reactions and death.

[0019] Administration method: (1) subarachnoid route: after successful anesthesia, t...

Embodiment 2

[0021] Embodiment 2 subacute toxicity test

[0022] 40 healthy adult Wistar rats (the same source as in Example 1), male or female, with a body weight of 210-270 g, were randomly divided into 4 groups (L1-L4) according to the injection site, 10 in each group, and measured body weight, white blood cell count, Red blood cell count and hemoglobin content, after successful intraperitoneal injection of 1% pentobarbital sodium (40mg / kg), the N6 compound was prepared immediately and injected into the subarachnoid of rats at L2 and 3 levels respectively at a therapeutic dose of 166 μL / kg Cavity, tail vein, muscle, abdominal cavity, the administration method is the same as that of Example 1. After 2 weeks, body weight, white blood cell count, red blood cell count and hemoglobin content were measured and statistically processed.

[0023] Experimental animals were treated in two ways: 5 animals were randomly selected from each group and killed, and the liver, spleen, heart and kidney wer...

Embodiment 3

[0027] Example 3 In Vitro Hemolysis Test

[0028] Take 7 test tubes and prepare N6 complex immediately. Inject 41.5 μL, 83 μL, 166 μL, 332 μL, and 664 μL of N6 complex into test tubes 1 to 5 respectively, add human erythrocyte suspension and normal saline in equal amounts, and use test tube 6 as a blank control. Test tube No. 7 was used as a complete hemolysis control, placed in a 37°C incubator, and observed for hemolysis at 1 hour, 2 hours, and 3 hours respectively.

[0029] After 3 hours of observation, there was no hemolysis in test tubes 1 to 5. The upper layer was a light pink clear liquid, and the lower layer was red blood cell deposits. No red blood cell aggregation or rupture was found in the smear microscope.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for intrathecally injecting a nerve regeneration promoting injection. The method is completed by injecting the nerve regeneration promoting medicine into the arachnoid membrane. The nerve regeneration promoting injection is prepared from the following components: 280-320mu g of mouse nerve growth factors for injection, 19-21ml of monosialotetrahexosyl ganglioside sodium injection, 8-22ml of cattle encephalon glycoside and ignotin injection, 9-11ml of mecobalamine injection and 9-11ml of dexamethasone acetate injection. The method is conductive to reducing secondary injury after spinal cord injury, and the intrathecal injection has an excellent biological safety.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for intrathecally injecting an injection for promoting nerve regeneration. Background technique [0002] Paraplegia caused by spinal cord injury is a disease that seriously affects the quality of life of the patient. The patient loses the ability to live independently, suffers great pain physically and mentally, and also brings a huge burden to the family and society. [0003] Spinal cord injury repair has always been a difficult problem for basic medical researchers and clinicians. Clinically, in addition to surgery for early spinal cord injury, the current drug treatment is still based on nerve growth factor and ganglioside, but intramuscular injection and intravenous administration have no obvious therapeutic effect, the reason may be that this type of molecular weight is large, It is not easy to pass through the blood-brain barrier, and cannot reach an ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/18A61K9/08A61P25/28A61K31/7032A61K31/714A61K31/573
Inventor 赵廷宝张晓君林世德刘鑫于大鹏尚明富
Owner 赵廷宝
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com