43 results about "Erythrocyte suspension" patented technology

Lysis / resealing process for incorporating an active ingredient, the process comprising the following steps: (1) - placing a globular concentrate in suspension in an isotonic solution having a haematocrit level which is equal to or greater than 65 %, with refrigeration at from + 1 to + 8 DEG C, (2) - measuring the osmotic fragility based on a sample of erythrocytes from that same globular concentrate, preferably on a sample of the suspension obtained in step (1), (3) - lysis and internalisation procedure of the active ingredient, inside the same chamber, at a temperature which is constantly maintained at from + 1 to + 8 DEG C, comprising allowing the erythrocyte suspension having a haematocrit level which is equal to or greater than 65 % and a hypotonic lysis solution which is refrigerated at from + 1 to + 8 DEG C, to circulate in a dialysis cartridge; the lysis parameters being adjusted in accordance with the osmotic fragility previously measured; and (4) - resealing procedure carried out in a second chamber at a temperature of from + 30 to + 40 DEG C by means of a hypertonic solution. Figure 1.

The present invention relates to a method for detecting substances specifically inhibiting a type III secretion mechanism and functions of the type III secretory proteins, within short time and large amounts thereof, without depending upon animal infectious experiments. Namely it relates to the method for detection of a type III secretory mechanism inhibitor comprising mixing a bacterium having the type III secretory mechanism and an erythrocyte suspension, adding the type III secretory mechanism inhibitor thereto, and detecting changes in the thus formed hemolytic activity. The method for detecting substances can be treated large amount of samples within short time by exhibiting the substances inhibiting the type III secretion mechanism or the functions of the type III secretory proteins as numerical index of the hemolytic activity of erythrocytes. Consequently, the present invention is useful for development of drugs.

A lysis / resealing process for preparing erythrocytes containing active ingredient is provided comprising placing a globular concentrate in suspension in an isotonic solution having a haematocrit level which is equal to or greater than 65%, with refrigeration at 1 to 8° C.; measuring the osmotic fragility based on a sample of erythrocytes from that same globular concentrate, preferably on a sample of the suspension; lysis and internalization procedure of the active ingredient, inside the same chamber, at a temperature maintained at 1 to 8° C., comprising allowing the erythrocyte suspension having a haematocrit level equal to or greater than 65% and a hypotonic lysis solution which is refrigerated at 1 to 8° C., to circulate in a dialysis cartridge; the lysis parameters being adjusted in accordance with the osmotic fragility previously measured; and resealing in a second chamber at a temperature of from 30 to 40° C. by means of a hypertonic solution.

The invention provides a method for detecting the mechanical characteristics of red blood cells based on an atomic force microscope, which relates to the technical field of biophysics. The technical scheme adopted in the invention is as follows: a round coverslip is used to fix red blood cells; and the method specifically includes the following steps: preparing red blood cell suspension, preprocessing the round coverslip, preparing an atomic force microscope sample, and using the atomic force microscope to detect the mechanical characteristics of the red blood cells in a liquid environment. The red blood cells are fixed by the round coverslip, so that the red blood cells can still be maintained in a physiological state, and the maintenance of the normal morphology and function of the red blood cells is very obvious. Necessary disinfection and innocuous treatment is carried out on the commonly used round coverslip, so that there is no additional drug effect and toxic effect on the red blood cells, and the morphology and function of the cells will not be affected. The red blood cells treated by the round coverslip can be stably attached, the mechanical characteristics of the red blood cells can be independently detected without any other influence or interference, and the real physiological characteristics of the cells can be reflected.

The invention provides a erythrocyte preservation solution. The composition and content of the erythrocyte preservation solution are as follows: adenine 0.3-0.5g / L, glucose 25-35g / L, sodium citrate 6-10g / L, inosine 0.2-0.4 g / L, chloramphenicol 0.2~0.3g / L, neomycin sulfate 0.08~0.12g / L, sodium chloride 2.5~3.5g / L, sodium dihydrogen phosphate 0.04~0.06g / L. The present invention can make the storage period of red blood cells reach 14 weeks, and within the storage period, the preserved red blood cell suspension of the present invention has higher antigen titer.

The invention relates to a method for measuring the electrical characteristics of a cell suspension by using a pulsed electro-acoustic method. According to the method of the invention, the fundamental principles of a traditional pulsed electro-acoustic method (PEA) for measuring the space charges of a solid thin film or a cable material are adopted to detect the charged characteristics of the cell suspension. The method includes the following steps that: 1) a three-layer composite media model is established, and the use conditions of the three-layer composite media model are defined; 2) red cells are separated from venous blood obtained from the tail of an SD rat, and the red cells are diluted to an appropriate concentration; 3) an obtained red cell suspension is injected into a measuring chamber; 4) direct-current bias voltage and pulse electrical signals are applied to the sample; and 5) acoustic waves are detected and converted into electrical signals through a piezoelectric sensor, so that the charged characteristics of the cell suspension can be calculated. The method of the invention has the advantages of simplicity, quickness and convenient operation. With the method adopted, the charged characteristics of various kinds of cells can be measured in a non-destructive manner, and a new measurement means can be provided for the study of electrophysiology.

The invention discloses a preparation method and a detection method of a goat pox reverse indirect hemagglutination diagnostic reagent capable of detecting goat pox antigen. The preparation method is: mix glutaraldehyde and sheep red blood cells to prepare red blood cell suspension, mix with the same amount of purified goat pox IgG, stir, centrifuge, wash, and use PBS containing 1% newborn calf serum to make 1% sensitization Red blood cells, this is the diagnostic reagent for goat pox reverse indirect hemagglutination. The optimized preparation conditions are: use glutaraldehyde monoformylation method to fix red blood cells, the concentration of sensitized red blood cells is 0.8%-1.0%, and the IgG content is controlled at 1.5-1.55mg.ml-1, which can achieve a better labeling effect; The reagent is used to detect goat pox virus antigen according to the conventional reverse indirect hemagglutination test method, the reaction temperature is 20-25°C, and the reaction time is 50min. The method has good specificity, sensitivity and repeatability, and can be used for rapid detection of goat pox antigen in veterinary clinic.

A process for the detection of antibodies in a test sample by preparing a suspension of erythrocytes with a test serum or plasma by mixing a test serum or plasma with erythrocytes; incubating the suspension of erythrocytes at a temperature of from 37° C. to 45° C. to bind any antibodies in the test serum or plasma to the surface of said erythrocytes; combining the suspension of erythrocytes with an amount of a solution of a macromolecule which is effective to agglutinate the erythrocytes; packing the resultant red cell agglutinates by centrifuging the suspension of erythrocytes; and, determining the presence of anti-erythrocyte antibodies by observing if antibody-dependent erythrocyte agglutination has occurred.

The invention discloses a method for building an oxidative stress model of carp red blood cells, which comprises sterilizing the tail of a carp, drawing about 1 millimeter of blood from the tail of each carp; putting the blood into 6 millimeters of carp saline solution containing heparin, centrifuging 900 grams of a blood sample at a temperature of 4 DEG C for 10 minutes, rejecting supernate; suspending red blood cell deposits in the 6 millimeters of carp saline solution, centrifuging 900 grams of sample at a temperature of 4 DEG C for 10 minutes; re-suspending the red blood cell deposits in the carp saline solution, washing suspending the red blood cell deposits in the 6 millimeters of carp saline solution after the red blood cell deposits are washed twice, preserving the red blood cell deposits at a temperature of 4 DEG C for reserve; drawing adequate red blood cell suspension, adding equal volume of Fe2+ mixture solution with a density of 40 mu M or H2O2 mixture solution with a density of 20 mu M, incubating the suspension at a temperature of 37 DEG C for 8 to 10 hours; centrifuging 1000 grams of sample at a temperature of 4 DEG C for 3 minutes, and detecting apoptosis of cell samples by a flow cytometry and agarose gel electrophoresis. The method for building the oxidative stress model of carp red blood cells utilizes the Fe2+ or H2O2 mixture solution as a source of free radical OH, for the first time, and a red blood cell oxidative stress model is built.

The present invention discloses an experimental method for evaluating eye irritation by using miniature pig erythrocytes. The method mainly comprises: (1) preparing a suspension of miniature pig erythrocytes; (2) carrying out a miniature pig erythrocyte hemolysis test; (3) carrying out a protein denaturation experiment; (4) predicting a model; (5) carrying out classification and judgment. According to the present invention, the source of the erythrocytes is reliable and safe; the tested material is easy to obtain, and has good uniformity; the test system is simple and cheap, and no special equipment is required; the detection method is rapid, sensitive and universal; the method of the present invention can be directly applicable for safety evaluations of chemicals, cosmetics, pesticides, eye medicines and other substances possibly contacting the eyes, wherein the chemicals comprise surfactants, disinfection products, detergent and the like, the cosmetics comprise shampoo, bath foam, eye cream and the like; the method further can be applicable for rapidly screening, classifying and identifying the eye irritations of the raw materials, the formulas or the products.

The invention discloses a method for detecting malaria parasites, which comprises the following steps: providing a sample to be tested, and processing the sample to obtain a red blood cell suspension; preparing a saponin-containing lysate; mixing the red blood cell suspension with the lyse and fully After lysis, centrifuge and retain the first precipitate, then fully lyse the first precipitate, then centrifuge and retain the second precipitate, then wash the second precipitate with saline at least three times, then centrifuge and retain the third precipitate; use Raman laser irradiation The third step is to precipitate and collect the Raman signal; and analyze the Raman signal to determine whether the sample to be tested contains Plasmodium. This method of detection of malaria parasites releases malaria parasites from red blood cells through saponin, and then obtains malaria pigment by lysing the plasma parasite cell membrane, and concentrates malaria parasites. Combined with Raman spectroscopy, the detection sensitivity is high. The invention also discloses a malaria parasite detection system.