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Combined rapid detection method for various avian antibodies

A detection method and antibody technology are applied in the field of combined rapid detection of various poultry antibodies, which can solve the problems of heavy repetitive sample addition and poor accuracy of detection technicians, and achieve the effects of low cost, high accuracy and simple operation.

Pending Publication Date: 2020-05-26
内蒙古正大食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a combined rapid detection method for various poultry antibodies, to at least solve the problems in the prior art that detection technicians repeatedly add samples to heavy work and poor accuracy

Method used

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  • Combined rapid detection method for various avian antibodies
  • Combined rapid detection method for various avian antibodies
  • Combined rapid detection method for various avian antibodies

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Step 1: Use an 8-channel micropipette to add 0.125 mL of physiological saline to the wells in the 1st to 10th columns of the 96-well original reaction plate 1.

[0025] Step 2: Use a single-channel micropipette to pipette 0.125mL of the serum solution to be tested to each well of the first column of the original reaction plate 1, and repeatedly pipette 5 times to mix.

[0026] Step 3: Use an 8-channel micropipette to draw 0.125mL from the wells of the first column of the original reaction plate 1, pipette and mix 5 times repeatedly, and then transfer to the wells of the second column. Pipette 0.125 mL into the wells and discard.

[0027] Step 4: If figure 1 As shown, transfer the serum to be tested diluted in step 3 into corresponding wells of 96-well ND reaction plate 2, H9 reaction plate 3, H5 reaction plate 4, and H7 reaction plate 5 at 0.025 mL per well.

[0028] Step 5: Add 0.025 mL of normal saline to the wells in column 11 of each reaction plate in step 4, and ...

Embodiment 2

[0037] Step 1: Use an 8-channel micropipette to add 0.125 mL of PBS to the 1st to 10th column wells of the 96-well original reaction plate 1.

[0038] Step 2: Use a single-channel micropipette to pipette 0.125mL of the serum solution to be tested to each well of the first column of the original reaction plate 1, and repeatedly pipette 5 times to mix.

[0039] Step 3: Use an 8-channel micropipette to draw 0.125mL from the wells of the first column of the original reaction plate 1, pipette and mix 5 times repeatedly, and then transfer to the wells of the second column. Pipette 0.125 mL into the wells and discard.

[0040] Step 4: If figure 1 As shown, transfer the serum to be tested diluted in step 3 into corresponding wells of 96-well ND reaction plate 2, H9 reaction plate 3, H5 reaction plate 4, and H7 reaction plate 5 at 0.025 mL per well.

[0041] Step 5: Add 0.025mL PBS to the wells in column 11 of each reaction plate in step 4, and add 0.05mL PBS to the wells in column 1...

Embodiment 3

[0045] Step 1: Use an 8-channel micropipette to add 0.1 mL of physiological saline to the wells in the 1st to 10th columns of the 96-well original reaction plate 1.

[0046] Step 2: Use a single-channel micropipette to pipette 0.1mL of the serum solution to be tested to each well of the first column of the original reaction plate 1, and repeatedly pipette 5 times to mix.

[0047] Step 3: Use an 8-channel micropipette to draw 0.1mL from each well of the first column of the original reaction plate 1 and pipette repeatedly 5 times to mix well, then transfer to the wells of the second column, and sequentially dilute to the wells of the 10th column. Pipette 0.1 mL into the wells and discard.

[0048] Step 4: If figure 1 As shown, transfer the serum to be tested diluted in step 3 into corresponding wells of 96-well ND reaction plate 2, H9 reaction plate 3, H5 reaction plate 4, and H7 reaction plate 5 at 0.02 mL per well.

[0049] Step 5: Add 0.02 mL of normal saline to the wells i...

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Abstract

The invention belongs to the technical field of antibody detection, and provides a combined rapid detection method for various avian antibodies, which comprises: step 1, adding 0.1-0.2 mL of a diluentto all holes in first to tenth columns of an original reaction plate; step 2, adding 0.1-0.2 mL of serum liquid into each hole in the first column in the step 1; step 3, sucking 0.1-0.2 mL from eachhole in the first column in the step 2 into the holes in the second column; step 4, respectively transferring the serum liquid obtained in the step 3 into corresponding reaction holes of ND, H9, H5 and H7 reaction plates according to 0.02-0.04 mL per hole; step 5, respectively adding 0.02 to 0.04 mL of diluent into each hole in the eleventh column of each reaction plate in the step 4; step 6, adding 0.02-0.04 mL of ND, H9, H5 and H7 virus antigen solutions into the holes in the first to eleventh columns of the reaction plates in the step 5; step 7, adding 0.02-0.04 mL of chicken erythrocyte suspension into each hole of each reaction plate in the step 6, and judging the antibody titer of ND, H9, H5 and H7; meanwhile, the invention has the characteristic that multiple antibodies can be simultaneously detected by a single person.

Description

technical field [0001] The invention belongs to the technical field of antibody detection, and in particular relates to a combined rapid detection method of various poultry antibodies. Background technique [0002] Some viruses contain hemagglutinin on the surface, which can bind to mucin receptors on the surface of chicken erythrocytes, causing erythrocytes to agglutinate, which is called agglutination (HA). This agglutination phenomenon can be inhibited by specific immune serum, which is called hemagglutination inhibition (HI). Through the HA-HI test, known viruses can be used to check the corresponding antibody content in the tested serum. The test is suitable for the detection of Newcastle disease, avian influenza and egg drop syndrome antibody titers in serum. [0003] However, the existing HA / HI method can only detect one poultry disease antibody. Usually, poultry farms need to detect multiple antibodies such as Newcastle disease, avian influenza, and egg drop syndrom...

Claims

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Application Information

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IPC IPC(8): G01N33/536G01N33/68
CPCG01N33/536G01N33/6854
Inventor 刘利军王建华王静丽郝瑞军
Owner 内蒙古正大食品有限公司
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