Rapid detection kit for IgG blood group antibody titer

Abstract

The invention discloses a rapid detection kit for IgG blood group antibody titer. The rapid detection kit comprises a standard red blood cell suspension, a flushing fluid and a detection test strip; the detection test strip comprises a sample adding part, a reaction part and a water absorption part which are sequentially arranged in a contact manner from the upstream side to the downstream side; the sample adding part is sequentially provided with a washing sample adding area, an erythrocyte sample adding area and a sample adding area from the upstream side to the downstream side; the reactionpart is provided with at least one detection point and a contrast point, wherein at least one detection point is coated and cured with an anti-human IgG antibody, and the contrast point is coated andcured with an anti-RBC antibody. When the kit is used, only one-step sample adding, red blood cell adding and flushing fluid adding are needed, and a result can be obtained after 3-5 minutes.

Description

technical field [0001] The invention belongs to the technical field of blood type detection, and in particular relates to a kit for rapid detection of IgG blood type antibody titer. Background technique [0002] Hemolytic disease of the newborn (HDN) is one of the most common acute hemolytic diseases in pediatrics. IgG antibody is the main type, and this IgG antibody can enter the fetus through the placenta, destroying fetal red blood cells and causing HDN. In severe cases, miscarriage, premature delivery, stillbirth, hemolytic jaundice, severe anemia, and nuclear jaundice may develop. It can cause early death of infants or leave survivors with irreversible neurological sequelae, bringing great pain to children and their families. Among the human blood group systems discovered so far, hemolytic disease of the newborn caused by ABO blood group incompatibility is the most common, followed by the Rh blood group system, and HDN caused by other blood group systems is relatively ...

AI Technical Summary

Problems solved by technology

But it still has many problems and deficiencies, such as, 1) the production process is complicated, artificial blood group antigen needs to be prepared, and tracer particles are needed for labeling; 2) the process is not mature, artificial blood group antigen is only a theoretical concept, and its practical application is almost impossible. Zero, specificity, affinity, and stability cannot be studied. 3) The result interpretation is not easy, and there are 3-100 lines in the result interpretation area, including many coated lines in the block area, detection area, and quality control area, etc., 4 ) is still inconvenient to operate. When there is no blocking zone, it is necessary to use traditional chemical methods to inactivate IgM antibodies in advance, and then measure the processed samples. 5) The detection time is relatively long and takes more than ten minutes, because the test strip is based on traditional The low pore size nitrocellulose membrane chromatography strip, and the result interpretation area located on the nitrocellulose membrane is very long, the sample passes for a long time, and the release of tracer particle-labeled substances also takes time. 6) The types of detection antibodies are limited, only Measures IgG anti-A and anti-B antibodies but not IgG anti-D

Images