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Indirect anti-human globulin accelerated blood matching test method

A technology of antihuman globulin and test method, which is applied in the field of indirect antihuman globulin accelerated blood matching, can solve the problems of low reaction sensitivity, adverse reactions of blood transfusion, long incubation time, etc., achieve high sensitivity, improve sensitivity and accuracy strong effect

Inactive Publication Date: 2018-09-18
SECOND AFFILIATED HOSPITAL SECOND MILITARY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The weakness of this method is that the incubation time is long, the reaction sensitivity is low, and it needs to be incubated at 37°C for 30 to 60 minutes, and antibodies with weaker titers may be missed, resulting in serious adverse reactions to blood transfusions
When the patient is in urgent need of blood, the long-term operation of this method may cause greater danger

Method used

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  • Indirect anti-human globulin accelerated blood matching test method
  • Indirect anti-human globulin accelerated blood matching test method
  • Indirect anti-human globulin accelerated blood matching test method

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Experimental program
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Effect test

Embodiment 1

[0076] An indirect antiglobulin test acceleration enhancer provided by the present invention comprises a polyethylene glycol (PEG) solution with a molecular weight of 2000 and a 0.5% polybrene solution with a concentration of 5%, said The volume ratio of polyethylene glycol solution and polybrene solution is 10:1.

[0077] The preparation method of described indirect antihuman globulin test accelerated enhancer, comprises the following steps:

[0078] Dissolve the polyethylene glycol powder in distilled water to make a 5% polyethylene glycol (PEG) solution, and dissolve the polybrene powder in distilled water to make a 0.5% polybrene solution , then the polybrene solution is added to the polyethylene glycol solution, the volume ratio of the polyethylene glycol solution and the polybrene solution is 10:1, and the pH value of the solution is adjusted to 7 with hydrochloric acid or sodium hydroxide to obtain the described Accelerated enhancer for indirect antiglobulin test. The...

Embodiment 2

[0080] An indirect antiglobulin test acceleration enhancer provided by the present invention comprises a polyethylene glycol (PEG) solution with a molecular weight of 8000 at a concentration of 20% and a polybrene solution at a concentration of 2.5%. The volume ratio of polyethylene glycol solution and polybrene solution is 20:1.

[0081] The preparation method of described indirect antihuman globulin test accelerated enhancer, comprises the following steps:

[0082] Dissolve the polyethylene glycol powder in distilled water to make a 20% polyethylene glycol (PEG) solution, and dissolve the polybrene powder in distilled water to make a 2.5% polybrene solution , then the polybrene solution is added to the polyethylene glycol solution, the volume ratio of the polyethylene glycol solution and the polybrene solution is 20:1, and the pH value of the solution is adjusted to 7 with hydrochloric acid or sodium hydroxide to obtain the described Accelerated enhancer for indirect antigl...

Embodiment 3

[0084] A kind of indirect antihuman globulin test accelerated enhancer provided by the present invention, comprises the polyethylene glycol (PEG) solution that concentration is 1%, molecular weight is 5000 and the concentration is the polybrene (polybrene) solution that concentration is 0.1%, described The volume ratio of polyethylene glycol solution and polybrene solution is 15:1.

[0085] The preparation method of described indirect antihuman globulin test accelerated enhancer, comprises the following steps:

[0086] Dissolve the polyethylene glycol powder in distilled water to make a 1% polyethylene glycol (PEG) solution, and dissolve the polybrene powder in distilled water to make a 0.1% polybrene solution , then the polybrene solution is added to the polyethylene glycol solution, the volume ratio of the polyethylene glycol solution and the polybrene solution is 15:1, and the pH value of the solution is adjusted to 7 with hydrochloric acid or sodium hydroxide to obtain the...

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Abstract

The invention discloses an indirect anti-human globulin accelerated blood matching test method, which comprises: adding physiological saline to red blood cells, washing, uniformly mixing, carrying outcentrifugal separation, removing the supernatant, adding physiological saline, preparing a red blood cell suspension, adding plasma or serum, uniformly mixing, adding an indirect anti-human globulintest acceleration enhancer, uniformly mixing, incubating, adding physiological saline, carrying out centrifugal separation, slightly shaking the test tube, observing the result, and judging the resultaccording to the red blood cell agglutination or dispersion condition. According to the present invention, the cross matching test and / or the red blood cell irregular antibody screening test before the blood transfusion is accelerated and enhanced by using the indirect anti-human globulin test acceleration enhancer, and the indirect anti-human globulin test acceleration enhancer has characteristics of short incubation time, high sensitivity and strong accuracy, can be quickly detect various weak red blood cell irregular antibodies, and is suitable for emergency rapid cross-matching and red blood cell irregular antibody screening.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a test method for indirect antiglobulin accelerated blood matching, which can accelerate the speed of blood matching or antibody screening. Background technique [0002] The ABO blood group system is the first human blood group system discovered by Landsteiner in 1900. With further research on blood types, 36 human blood group systems and more than 300 blood group antigens have been discovered so far. Incompatible transfusions may result in severe hemolytic reactions and even shock leading to death. At present, the ABO blood group and Rh blood group detected routinely in clinical practice are the two most important blood group systems, but if the remaining 34 blood group systems do not match, adverse transfusion reactions caused by antigens and antibodies may still occur. Therefore, cross-matching blood is particularly important before blood transfusion, which can reduce the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/559
CPCG01N33/559
Inventor 周晔唐晓峰王雪琦蒋天舒沈磊黄斐
Owner SECOND AFFILIATED HOSPITAL SECOND MILITARY MEDICAL UNIV
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