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Method for evaluating eye irritation by using miniature pig erythrocytes

A system evaluation, miniature pig technology, applied in the direction of color/spectral property measurement, material inspection products, biological testing, etc., can solve problems such as animal suffering, lack of relevant patents and literature reports, and differences in animal and human responses, achieving sensitive High degree, meet the needs of routine toxicity detection and mechanism research, and have good correlation effect

Inactive Publication Date: 2013-05-22
程树军 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Disadvantages of animal experiments include: subjectivity of the method, differences in responses between animals and humans, extreme suffering for animals, etc.
At present, there are no relevant patents and literature reports for the in vitro method established based on the standardized experimental animal minipig red blood cell system

Method used

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  • Method for evaluating eye irritation by using miniature pig erythrocytes
  • Method for evaluating eye irritation by using miniature pig erythrocytes
  • Method for evaluating eye irritation by using miniature pig erythrocytes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0187] 1. Preparation of Minipig Red Blood Cell Suspension

[0188] 1.1 Miniature pigs

[0189]The blood comes from closed group Wuzhishan miniature pigs, and the anticoagulant blood comes from Wuzhishan miniature pigs raised in a standardized manner. The genetic background is clear, the population is stable, and the source is reliable, which can meet the needs of testing. The healthy and stable source of red blood cells; , age and weight are uniformly controlled, and the difference between batches is small to ensure stable and reliable test results; the damage effect of chemicals on the cell membrane is quantitatively evaluated by red blood cell hemolysis; A predictive model was established to evaluate the eye irritation of chemical substances.

[0190] 1.2 Preparation of minipig red blood cell suspension

[0191] 1.2.1 Experimental materials

[0192] PBS buffer solution, PBS buffer solution contains the following components: based on 1L of buffer solution, sodium chl...

Embodiment 2

[0226] 1. Preparation of Minipig Red Blood Cell Suspension

[0227] 1.1 Miniature pigs

[0228] The blood material comes from a closed herd of Wuzhishan miniature pigs native to Wuzhishan District, Hainan Province.

[0229] 1.2 Preparation of minipig red blood cell suspension

[0230] 1.2.1 Experimental materials

[0231] PBS buffer solution: pH 7.3, stored at 4°C, PBS buffer solution contains the following components: based on 1L of buffer solution, sodium chloride 8.0g, potassium chloride 0.2g, glucose 1.8g, anhydrous disodium hydrogen phosphate 1.15g, Anhydrous potassium hydrogen phosphate 0.2 g, the rest is deionized water.

[0232] 5mL anticoagulant tube: containing K 2 -EDTA.

[0233] Positive control solution: Prepare a sodium dodecyl sulfate (SDS) solution with a concentration of 10 g / L in PBS.

[0234] 1.2.2 Test substance: Benzophenone is a fragrance fixative, which can be used as a raw material for cosmetics, and can also be used to manufacture antihistamines...

Embodiment 3

[0264] 1. Preparation of Minipig Red Blood Cell Suspension

[0265] 1.1 Miniature pigs

[0266] Tibetan miniature pigs are native to the Nyingchi region of Tibet and later turned into experimental animals. They are standardized experimental animals with known genetic background, clear microbial background, and stable population.

[0267] 1.2 Preparation of minipig red blood cell suspension

[0268] 1.2.1 Experimental materials

[0269] PBS buffer solution: pH 7.2, stored at 4°C, PBS buffer solution contains the following components: based on 1L buffer solution, 8.0g sodium chloride, 0.2g potassium chloride, 1.8g glucose, 1.15g anhydrous disodium hydrogen phosphate, Anhydrous potassium hydrogen phosphate 0.2 g, the rest is deionized water.

[0270] 5mL anticoagulant tube: containing citrate buffer, in which the concentration of sodium citrate in the citrate buffer is 66 mmol / L, and the concentration of citric acid is 44 mmol / L, prepared with ultrapure water.

[0271] Posit...

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Abstract

The present invention discloses an experimental method for evaluating eye irritation by using miniature pig erythrocytes. The method mainly comprises: (1) preparing a suspension of miniature pig erythrocytes; (2) carrying out a miniature pig erythrocyte hemolysis test; (3) carrying out a protein denaturation experiment; (4) predicting a model; (5) carrying out classification and judgment. According to the present invention, the source of the erythrocytes is reliable and safe; the tested material is easy to obtain, and has good uniformity; the test system is simple and cheap, and no special equipment is required; the detection method is rapid, sensitive and universal; the method of the present invention can be directly applicable for safety evaluations of chemicals, cosmetics, pesticides, eye medicines and other substances possibly contacting the eyes, wherein the chemicals comprise surfactants, disinfection products, detergent and the like, the cosmetics comprise shampoo, bath foam, eye cream and the like; the method further can be applicable for rapidly screening, classifying and identifying the eye irritations of the raw materials, the formulas or the products.

Description

technical field [0001] The invention relates to a method for evaluating eye irritation by using minipig erythrocyte system. Background technique [0002] Eyes are often exposed to a variety of substances, including cosmetics, pharmaceuticals, pesticides, and environmental pollutants. Subjective contact or accidental exposure of these foreign substances may cause health risks to the population. Therefore, evaluating and investigating the potential eye irritation of cosmetics / chemicals has become a prerequisite for ensuring the safe use of cosmetics and daily chemicals. In the 1930s, untested eyelash products containing p-phenylenediamine were allowed to be sold in the US market, causing consumers to use such products to cause eye allergies, corneal ulcers and blindness. These public hazards prompted the U.S. Food, Drug and Cosmetic Administration (FDA) to take action in 1938, mandating testing of eye contact products for safety. [0003] For the toxicological evaluation of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31G01N33/50G01N33/72
Inventor 程树军敖华英黄韧王希龙谈伟君
Owner 程树军
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