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Erythrocyte preservative solution

A technology of red blood cells and preservation solution, which is applied in the field of biotechnology materials, can solve the problems of bacteria growth and preservation effect, and achieve the effects of long preservation time, normal metabolism protection and invasion prevention

Active Publication Date: 2015-11-11
苏州苏大赛尔免疫生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the storage period of GMA red blood cell preservation solution is only 42 days, and the preserved red blood cells will grow bacteria in about 4 weeks, and the preservation effect is greatly affected.

Method used

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  • Erythrocyte preservative solution
  • Erythrocyte preservative solution
  • Erythrocyte preservative solution

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0025] Preparation of washed red blood cells:

[0026] Aspirate 5 parts of fresh red blood cells from type A healthy people and mix them, add 2 to 3 times the volume of sodium oxide injection, blow and mix well, put them in a centrifuge at 3000rpm for 5 minutes, and take out the centrifuged type A red blood cell test tube , absorb the supernatant and white blood cells, platelets and other components attached to the surface of red blood cells, then add 2 to 3 times the volume of sodium oxide injection, mix well, and centrifuge at 3000rpm for 5 minutes in a centrifuge, and repeat twice. After the 4th centrifugation, aspirate most of the supernatant, leaving the packed red blood cells for later use. Aspirate 100 μl of the washed red blood cells into a dropper bottle that has been added with 9.9 mL of red blood cell preservation solution, and prepare 2 bottles of 10 mL of 1% red blood cell suspension preserved in different preservation solutions, one of which is used for detection...

Embodiment 1

[0029] Prepare red blood cell preservation solution according to the following components and contents:

[0030]

[0031] The erythrocyte preservation solution of Example 1 was prepared together with the washed erythrocytes into a 1% erythrocyte suspension.

Embodiment 2

[0033] Prepare red blood cell preservation solution according to the following components and contents:

[0034]

[0035] The erythrocyte preservation solution of Example 2 was prepared together with the washed erythrocytes into a 1% erythrocyte suspension.

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Abstract

The invention provides an erythrocyte preservative solution. The solution comprises the following components by content: 0.3-0.5g / L of adenine, 25-35g / L of glucose, 6-10g / L of sodium citrate, 0.2-0.4g / L of inosine, 0.2-0.3g / L of chloramphenicol, 0.08-0.12g / L of neomycin sulfate, 2.5-3.5g / L of sodium chloride and 0.04-0.06g / L of sodium dihydrogen phosphate. According to the preservative solution, the preservation period of erythrocytes can be 14 weeks, and the erythrocyte suspension preserved in the solution has a relatively high antigen titer in the preservation period.

Description

technical field [0001] The invention relates to biotechnology materials, in particular to a red blood cell preservation solution. Background technique [0002] There are many kinds of antigens on the surface of red blood cell membrane, such as ABH antigen, Rh antigen and so on. These antigens can specifically bind to antibodies, so red blood cells are widely used in blood group serology tests. At present, red blood cells are obtained from human blood cells and cannot be replaced by substances from other sources, which greatly limits the acquisition and preservation of red blood cells. If fresh red blood cells are placed in normal saline, hemolysis will occur within 1 to 2 days, making them unusable. Therefore, the preservation method of red blood cells has always been a concern of relevant researchers. Especially for some rare blood types, due to the scarcity of sources and short storage time, it has greatly hindered the smooth progress of blood type serological tests. ...

Claims

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Application Information

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IPC IPC(8): A01N1/02G01N33/80
Inventor 费敏钱小红张晓丽唐亚犁
Owner 苏州苏大赛尔免疫生物技术有限公司
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