130 results about "Neomycin Sulfate" patented technology

This invention relates to a method of treating otitis externa using a topical combination medication, including one or more antifungal agent such as, for example fluconazole, voriconazole, itraconazole, clotrimazole, amphotericin B, caspofungin, micafungin, terbinafine, naftifine, butenafine, amorolfine, ravuconazole, posaconazole, flucytosine, econazole, enilaconazole, miconazole, oxiconazole, saperconazole, sulconazole, terconazole, tioconazole, nikkomycin Z, anidulafungin (LY303366), nystatin, pimaricin, griseofulvin, ciclopirox, haloprogin, tolnaftate, and undecylenate and one or more antibacterial agent such as neomycin sulfate, polymyxin B sulfate, colistin sulfate, gentamycin, tobramycin, chloramphenicol, Ciprofloxacin, Ofloxacin, a penicillin compound, a cephalosporin compound, a macrolide compound, a fluoroquinolone compound, streptomycin, or kanamycin.

The invention provides an erythrocyte preservative solution. The solution comprises the following components by content: 0.3-0.5g / L of adenine, 25-35g / L of glucose, 6-10g / L of sodium citrate, 0.2-0.4g / L of inosine, 0.2-0.3g / L of chloramphenicol, 0.08-0.12g / L of neomycin sulfate, 2.5-3.5g / L of sodium chloride and 0.04-0.06g / L of sodium dihydrogen phosphate. According to the preservative solution, the preservation period of erythrocytes can be 14 weeks, and the erythrocyte suspension preserved in the solution has a relatively high antigen titer in the preservation period.

The invention relates to a red cell storage solution and a preparation method thereof. The storage solution comprises a buffering system, a nutritional system, an osmotic pressure system and an anticorrosion system, wherein the anticorrosion system comprises the following components according to mass concentration: 0.2-1g / L of chloramphenicol, 0.1-0.6g / L of neomycin sulfate, and 0.1-0.5g / L of gentamicin. Through mutual concurrent effects of the buffering system, the nutritional system, the anticorrosion system and the osmotic pressure system of the red cell storage solution provided by the invention, long storage cycle of the red cell storage solution is ensured, at the end of the sixth month of the storage, that red cell suspension free hemoglobin concentration still conforms to national standard is detected, and the hemolysis ratio at the end of the storage is smaller than 0.8 percent of the total of red cells; the red cell storage solution provided by the invention can ensure relatively strong antigenic activity and stability of the red cells.

The invention discloses a compound dimetridazole premix for preventing and treating the poultry enterovirus syndrome. The premix is prepared by uniformly mixing the following components in percentage by weight: 10 to 30 percent of dimetridazole, 4 to 10 percent of neomycin sulfate, 2 to 8 percent of decoquinate econazole nitrate, 10 to 30 percent of lysozyme, 0.5 to 5 percent of etamsylate, 3 to 10 percent of lactic acid TMP and the balance of pharmaceutically applicable auxiliary material. According to the invention, the novel high-efficiency anticoccidial drug decoquinate econazole nitrate, the dimetridazole and the neomycin sulfate which have a special curative effect on intestinal infection, the lysozyme with effects of resisting to bacteria, resisting to viruses, diminishing inflammation, relieving pain and repairing intestinal mucosa, the high-efficiency hemostatics etamsylate and the drug synergist are compounded into a compound preparation; and the compound dimetridazole premix has a high-efficiency treatment effect on the poultry enterovirus syndrome. The compound dimetridazole premix has a reasonable and scientific formula, is simple to prepare, has wide application and can be used as a priority drug for preventing and treating the poultry enterovirus syndrome.

The invention discloses a method for producing neomycin sulfage. The method comprises the steps that a spore suspension liquid is prepared by inoculating and culturing spores, then the spore suspension liquid is subjected to fermentation, absorption, desorption, concentration and refining, and the neomycin sulfate is formed. When a basal culture medium is prepared and fermented, starch is utilized to replace rice flour in the traditional processing in the proportion of 5%; and by means of the measure, the material loss can be reduced, the labor intensity is reduced, the work environment can be improved, and the production can be powerfully guaranteed.

The invention provides a solution for sealing and preserving magnetic nanoparticles. The solution using water as solvent contains, calculated by 1L of the solution, Tris 0.01mol, bovine gamma-globulin 0.5 to 5g, fish skin gelatin 1 to 10g, new-born calf serum 1 to 10mL, emulsifying agent Tween 0.2 to 5g, and neomycin sulfate 0.01 to 0.5g. Compared with the prior art, the solution provided by the invention can effectively reduce nonspecific adsorption of the magnetic nanoparticles in immunoreaction when being used for sealing and preserving magnetic nanoparticles coated with antigens or antibodies, thereby improving the sensitivity of a reagent for determining chemiluminiscence of the magnetic nanoparticles.

The invention provides a nano-gold-based method for visually and rapidly detecting antibiotics in milk. The method disclosed by the invention is mainly characterized in that pyrocatechol violet is used for reducing chloroauric acid to prepare the nano-gold. In a nano-gold synthesizing process, the addition of the antibiotics (including kanamycin mono-sulfate, neomycin sulfate, streptomycin sulfate and bleomycin sulfate) influences the synthesis of the nano-gold so that the color is changed; and the antibiotics can be quantitatively detected through naked eyes and an ultraviolet visible absorption spectrometer. According to the method, the operation is simple and rapid, the sensitivity is high, the selectivity is good, and the method can be applied to the detection of the antibiotics in the milk.

The invention discloses a method for extracting neomycin sulfate from neomycin sulfate fermentation broth, and relates to a preparation method of a medicine raw material. The extraction method comprises filtering, resin absorption, prewashing, desorption, decolorization, desalination, salt conversion and spraying drying to obtain a finished product. The method has the following benefits: the problems of low static adsorption quantity, high cost, water resource waste, environmental pollution, low yield of finished neomycin sulfate products and high neomycin sulfate C content in the prior art are solved, and the method has the advantages of high production efficiency, low cost, little environmental pollution and good quality of the finished products.

The invention relates to a method for extracting neomycin from a neomycin broth. The method comprises the following steps: 1) heating the neomycin broth to temperature of 65-75 DEG C; 2) placing a diatomite filter aid with mass accounting for 1-2% of that of the neomycin broth in the heated neomycin broth; 3) filtering the neomycin broth prepared in the step 2) with a filter to obtain a clear liquid containing neomycin; 4) loading the clear liquid containing neomycin to directly carry out column adsorption; and 5) desorbing the clear liquid and concentrating the clear liquid to prepare the neomycin finished product. The method for extracting neomycin from the neomycin broth can solve the environmental protection problem in neomycin production, energy saving for the novel extraction technology for neomycin sulfate can be realized, and cleaning production can be realized.

The invention belongs to a compound veterinary medicine preparation for treating avian enteritis type colibacillosis in veterinary medicine. The preparation consists of three main medicinal components and an adjuvant; three main medicinal components include 3.25%W / W of neomycin sulfate, 0.81%W / W of lactic acid TMP (Trimethoprim) and 10%W / W of glutamine; and the adjuvant includes 85.94%W / W of glucose. The veterinary medicine preparation is utilized to treat the avian enteritis type colibacillosis. A processing technology of the composition is as follows: respectively grinding, screening and accurately weighing the components according to the necessary ratio, mixing for 25 min, and performing semi-finished product quality control, split charging, packaging and finished product quality control to obtain the composition. An application method is as follows: drinking the water in 1% level of the composition, and a course of treatment lasts for 4 days. The compound veterinary medicine preparation provided by the invention is remarkable in efficacy of avian enteritis type colibacillosis, low in drug resistance, safe and low toxicity, thereby having good popularization and application value.

The invention discloses Chinese medicinal oral liquid combining with ciprofloxacin or neomycin sulfate capable of eliminating medicine resistance plasmid in chicken colibacillosis, which is prepared from the following raw material medicines: 45 to 55 grams of astragalus, 45 to 55 grams of folium artemisiae argyi, 45 to 55 grams of root of red-rooted salvia, 45 to 55 grams of dandelion, 25 to 35 grams of rhubarb, 45 to 55 grams of sanguisorba officinalis, 45 to 55 grams of Chinese violet, 45 to 55 grams of artemisia apiacea, 25 to 35 grams of red paeony root, and 15 to 25 grams of liquoric root. The preparation process comprises: 1, sorting; 2, grinding; 3, soaking; 4, ultrasonic extraction; 5, concentration; 6, filtering; 7, adding anticorrosion agent; and 8, packing separately. The Chinese medicinal oral liquid has good treatment effect on infectious bursal disease, basically avoids residual and has high biological safety.

The invention belongs to the field of chemical detection, and particularly relates to an HPLC (high-performance liquid chromatography) detection method of a neomycin sulfate component and relevant substances. The HPLC detection method comprises the following steps: (1) testing a chromatographic condition and system applicability; (2) preparing a solution; and (3) determining. The HPLC detection method of the neomycin sulfate component and relevant substances is simple in operation and good in isolation, can detect two major components B and C as well as more relevant substances of neomycin, ishigh in detection efficiency, has good dedication performance, precision and accuracy, can detect more impurities compared with the prior art, can effectively control the components and relevant substances in the neomycin sulfate, and has high practicability.

The invention relates to a preparation method of a medicine raw material, in particular to a method for extracting sulfuric acid fra avermectin from fermentation broth of neomycin sulfate. The preparation method is as follows: fermentation broth of neomycin sulfate is adsorbed by strong acidic ion exchange resin, parsed, concentrated and decolorized; the concentrated and decolorized concentrated solution of the neomycin sulfate is adsorbed by weak acidic cation exchange resin; ammonia of 0.1 M to 1.0 M is used to elute the neomycin sulfate B in the saturated resin in the first procedure; and eluent is concentrated, acidified, decolorized, spray dried and micronized, and sulfuric acid fra avermectin is prepared. The preparation method solves the problems existing in the prior art that the analytical yield is low, the raw materials are consumed a lot, the cost is high as the methanol is used in the crystallization process, the methanol pollutes the environment, and a lot of organic solvent remains in the product. The preparation method has the advantages that the adsorption selectivity of the resin is improved, the used amount of the eluent is reduced, no organic solvent remains in the product, the environmental pollution is reduced, the yield is improved, the quality of the product is improved, and the production cost is low.

The invention relates to a neomycin sulfate fermentation method. The method is characterized by comprising the following steps of: (1) culturing bevel spores: preparing the bevel spores from sandy soil spores; (2) culturing a bevel spore suspension: culturing the bevel spores for 4-12 days at the temperature of 28+ / -2 DEG C, thereby preparing the bevel spore suspension; (3) culturing in a seeding tank: putting the bevel spore suspension in the seeding tank for culturing, wherein the mass content of peanut meal in the seeding tank is 1-2% the mass of culture fluid in the seeding tank; (4) culturing in a fermentation tank: feeding the culture fluid cultured by the seeding tank to the fermentation tank for culturing, wherein the mass content of the peanut meal in culture fluid in the fermentation tank is 0-4% the mass of the culture fluid in the fermentation tank; and (5) diluting fermentation broth into diluted fermentation broth, thereby completing the fermentation step. The neomycin sulfate fermentation method provided by the invention has the advantages that the problem of quality instability of peanut cakes can be solved, and the fermentation production of neomycin is effectively guaranteed.

The invention discloses an external medicinal composition for treating skin ulcers (bedsores, burn ulcers and crural region ulcers) and a preparation method thereof, and belongs to the technical field of medicaments. The compound refined white sugar formulation of the composition of the invention is a medicinal composition which is prepared by mixing the medicinal active ingredients such as refined white sugar, neomycin sulfate (or kanamycin sulfate or povidone iodine) and a medicinal diluent or a carrier. In a skin external medicine administration form, the formulation of the composition can be powder or ointment which is used for treating the skin ulcers such as the bedsores, the burn ulcers, the crural region ulcers and the like.

The invention discloses a composition for treating poultry intestinal bacteria and anaerobic bacteria mixing infection and a preparation method thereof, and aims to provide the composition for treating poultry intestinal bacteria and anaerobic bacteria mixing infection and the preparation method thereof. The composition can effectively treat mixed infection and reduce poultry reaction and is quick in effect and remarkable in effect. The composition is prepared from the following raw materials in percentage by weight: 5-15% of neomycin sulfate, 10-30% of dimetridazole, 5% of sodium chloride, 5% of potassium chloride and the balance of glucosum anhydricum. The composition disclosed by the invention adopts neomycin sulfate and dimetridazole as antibiotic components to remedy the deficiency of the antibiotic ranges of neomycin sulfate and dimetridazole and avoid therapeutic error. Combination of sodium chloride and potassium chloride has supplementing effect to electrolyte loss of diseased poultry caused by a great quantity of defecation and water drainage. The medicines are combined in use, so that the composition has the remarkable treatment effect on intestinal bacteria and anaerobic bacteria mixing infection.

The present invention provides the application of 2-methoxy estradiol, Neomycin sulfate and their mixture in inhibiting the nuclear translocation of angiogenesis factor. Animal test shows that each of 2-methoxy estradiol and Neomycin sulfate can inhibit the growth of human tumor cell in nude mouse and the mixture of them has even high effect. The present invention also provides the medicine composition containing 2-methoxy estradiol and Neomycin sulfate.

The invention discloses compound norfloxacin nicotinic soluble powder and a preparation method thereof, and provides soluble powder capable of treating poultry gastrointestinal infection and lowering drug resistance probability and the preparation method thereof. The compound norfloxacin nicotinic soluble powder is prepared from the following raw materials in percentage by weight: 3-10% of norfloxacin nicotinate, 5-15% of neomycin sulfate, 5% of sodium chloride, 5% of potassium chloride and the balance anhydrous glucose. In the invention, the norfloxacin nicotinate and the neomycin sulfate are taken as antibacterial ingredients, thus achieving double efficacies and effectively reducing the probability of bacterial drug resistance, wherein, the norfloxacin nicotinate can be absorbed into the bloodstream for killing pathogenic bacteria through circulation secretion, and the neomycin sulfate is rarely absorbed by oral administration and directly applied to intestinal tract for producing contact sterilization effect, so the norfloxacin nicotinate and the neomycin sulfate have good effect of killing the pathogenic bacteria resulting in the poultry gastrointestinal infection from the two aspects; and the combination of the sodium chloride and the potassium chloride can make up electrolyte loss caused by excessive diarrhea of infected poultry. Therefore, the combination of the drugs can strengthen the curative effect on the poultry bacterial gastrointestinal infection.

The invention provides a method for detecting detecting neomycin sulfate content in triamcinolone acetonide acetat. The method comprises the following steps: preparing bacterium suspension; preparinga standard solution; preparing a test solution; preparing a negative sample solution; culturing; detecting. The method is high in specificity and repeatability; compared with a liquid chromatography,the method has the advantages of being simple to operate, low in test cost, and convenient to popularize and apply; dichloromethane is treated as a solvent, so that the solubleness is high, and creamsubstrate can be quickly dissolved in dichloromethane; the influence of the cream substrate is greatly avoided; the detecting neomycin sulfate content detecting result is high in reliability and highin accuracy degree.

The invention relates to a method for detecting bifidobacterium in sour milk, which comprises the following steps of: preparing diluent, culture medium and antibiotic solution; treating and culturing a sample; counting a bacterial colony; and performing microscopic examination, Gram staining and catalase test. The antibiotic solution contains neomycin sulfate and paromomycin sulfate. The method of the invention can effectively eliminate the interference of other lactic acid bacteria, accurately detect the live bacteria number of the bifidobacterium in the sour milk containing 4 kinds of lactic acid bacteria, reduce the detection cost and improve the detection efficiency.

The invention relates to an external coating ointment for burns and scalds. The external coating ointment is prepared from the following medicines by using the following preparation methods: (1) preparing the following raw materials: 15g of furazolidone tablets, 3500 units of neomycin sulfate, 10g of a fluocinonide ointment, 5g of sesame oil, 15g of snake slough, 15g of astragalus membranaceus, 15g of radix stemonae, 15g of radix sophorae flavescentis and 10g of human urine agglomerate; (2) baking the snake slough, and decocting and concentrating the snake slough, the astragalus membranaceus, the radix stemonae and the radix sophorae flavescentis with a proper amount of water to obtain a thick medicine liquid; (3) mixing and crushing the human urine agglomerate, the furazolidone tablets and the neomycin sulfate into mixed medicine powder; (4) mixing the mixed medicine powder with the thick medicine liquid, the fluocinonide ointment and the sesame oil into mixed cream; and (5) enclosing the mixed cream into a glass bottle or an ointment shell and sealing. According to the external coating ointment for burns and scalds, the traditional Chinese medicines and the western medicines with the effects of efficiently killing insects, sterilizing, removing wind and expelling toxin are combined; a specific medicine human urine agglomerate is utilized to prepare the ointment; the ointment can be timely coated by a patient, and is capable of quickening wound healing, preventing and inhibiting tumor growth of the wound, inhibiting hyperblastosis, and healing and smoothing the wound.

The invention discloses application of aspartic acid serving as a neomycin sulfate synergist. According to application disclosed by the invention, the aspartic acid is found to serve as the synergist of antimicrobial medicine for the first time and especially serve as a synergist of neomycin sulfate to kill aeromonas hydrophila. In-vitro and in-vivo experiments show that combined utilization of the aspartic acid and the neomycin sulfate can greatly improve a killing or clearing effect on the aeromonas hydrophila, a treating effect on aeromonas hydrophila infection of fish can be improved by injecting or orally taking the two compositions, and the fish survival rate is remarkably improved. Therefore, the aspartic acid disclosed by the invention can serve as the synergist of the neomycin sulfate, can be combined with the neomycin sulfate to be applied to preparation of compound antibacterial medicine, not only improves a pesticide effect of the antibacterial medicine, but also remarkably reduces the use amount of medicine and further reduces side effects of the medicine and medicine residue.

The invention relates to medicinal granules for preventing and treating piglet diarrhea. The medicinal granules are mainly prepared from neomycin sulfate, norfloxacin hydrochloride, codonopsis pilosula, rhizoma atractylodis, agastache rugosa, elecampane, fried kudzu vine root and plantain. A preparation method for the granules comprises the following steps: respectively adding water into the codonopsis pilosula, the rhizoma atractylodis, the agastache rugosa, the elecampane, the fried kudzu vine root and the plantain and extracting; merging filtrate; performing alcohol precipitation by adding alcohol; taking supernatant; recovering the alcohol; concentrating the supernatant to prepare extractum; drying the extractum to form dry paste; crushing, screening, and uniformly mixing the screened dry paste with the neomycin sulfate, the norfloxacin hydrochloride and appropriate amount of auxiliary materials; and uniformly mixing the mixture with appropriate auxiliary materials to prepare the granules. The granules can effectively prevent and treat the piglet diarrhea.

The invention relates to the technical field of medicine preparation and in particular relates to nasal drops for treating rhinitis and a preparation method thereof. The nasal drops for treating the rhinitis are prepared from active components, auxiliary materials and a solvent, wherein the active components comprise chloramphenicol, dexamethasone sodium phosphate, chlorpheniramine maleate, ribavirin, metronidazole, lidocaine hydrochloride, borneol and neomycin sulfate; the auxiliary materials comprise a preservative, polyethylene glycol, a stabilizer and an osmotic pressure regulator. According to the nasal drops, the chloramphenicol, the dexamethasone sodium phosphate, the chlorpheniramine maleate, the ribavirin, the metronidazole, the lidocaine hydrochloride, the borneol and the neomycin sulfate are used as the active components, so that the nasal drops have the effects of freeing orifices and smoothening breath, relieving swelling and pain and repairing lesion tissues of nasal mucosa and have a good medicinal effect and people do not have dependence after the nasal drops are used for a long period of time.

The invention discloses a triamcinolone acetonide and neomycin paste and a preparation method thereof. The triamcinolone acetonide and neomycin paste comprises the following components in parts by weight: 6-10 parts of thymol crystals, 0.5-1.5 parts of triamcinolone acetonide acetate, 6-10 parts of neomycin sulfate, 260-300 parts of rubber, 125-135 parts of rosin, 20-25 parts of yellow soft paraffin, 10-15 parts of wool fat, 3-5 parts of liquid paraffin, 350-360 parts of lithopone, and 175-180 parts of zinc oxide. According to the invention, the preparation process of the triamcinolone acetonide and neomycin paste is simple, the procedures are few, the cost is low, the medicine efficacy is obvious, and the performance is stable.

The invention provides an ointment preparation as well as a preparation method and application thereof, belongs to the technical field of medicine preparations, and is used for solving the problem that a conventional skin injury medicine cannot have take effects of bleeding stopping, pain relieving, infection prevention and the like into account all generally. The ointment preparation comprises a matrix, polymyxin B sulfate, neomycin sulfate, bacitracin and hemostatic, wherein the polymyxin B sulfate, the neomycin sulfate, the bacitracin and the hemostatic are uniformly dispersed in the matrix. Due to synergism of multiple antibiotics in the ointment preparation, not only is a complementary antibacterial spectrum provided, but also the antibacterial range is widened, most skin bacterial infection pathogenic bacteria are covered, and moreover, due to the synergistic and overlapping effect of the antibiotics, the antibacterial function can be enhanced, and induced drug resistance can be avoided. In addition, the ointment preparation comprises local anesthetic and hemostatic, so that a prevention and treatment effect on partial skin bacterial infection can be achieved, functions of pain relieving and bleeding stopping of partial skin wound can be achieved, and patients can be greatly relieved from pain.

An analysis and detection method for neomycin sulfate relates to neomycin sulfate. The analysis and detection method for neomycin sulfate comprises the steps of synthesizing a gold nano-particle; preparing an Au@PVP core-shell nano-particle sol; weighing the Au@PVP core-shell nano-particle sol, and measuring a surface plasma resonance absorption spectrum of the Au@PVP core-shell nano-particle sol;and performing quantitative analysis and detection on neomycin sulfate. A hydrogen bond can be formed by using an amino group of neomycin sulfate and oxygen of pyrrolidone in PVP (Polyvinyl Pyrrolidone) on an Au@PVP core-shell nano-particle to induce agglomeration of the Au@PVP core-shell nano-particle, thereby causing reduction of the surface plasma resonance absorption at 542 nm and implementing the quantitative analysis and detection on neomycin sulfate. The method is simple in a sample pretreatment process, expensive instrument and equipment and higher operation technical requirements arenot needed and the requirement of rapid analysis and detection can be satisfied. The phenomenon that the nano-particle is hypersensitive to substances and thus agglomerates instantly is utilized; theanalysis and detection method is simple, rapid, visible and highly sensitive.

The invention relates to an extraction method of neomycin sulfate, comprising the steps of pre-treating neomycin fermentation broth, filtering the fermentation broth by micro-filtration membrane, concentrating the broth by nanofiltration membrane, crystallizing the broth in acetone solvent, obtaining crude neomycin by suction filtration, dissolving the crude neomycin in the water, salting the sulfuric acid, dewatering by activated carbon, and then obtaining neomycin sulfate product by fine filtering, spraying and drying. The extraction rate can be improved above 6% in such a manner that the crystallization technology is used for purifying and concentrating to extract the neomycin sulfate, and it is beneficial for improving the economic benefit. The method reduces wastewater discharge and is beneficial for environment protection. The method has small energy consumption without releasing and emitting pungent smell, and is beneficial for the occupational health of staffs on the position.

The invention relates to the technical field of seedling culture of vegetables and fruits, in particular to a successive-cropping-obstacle resisting culture method for netted melon seedlings. The successive-cropping-obstacle resisting culture method has the advantages that netted melon seeds are soaked in saturated salt water solution under a vacuum environment, soaked in fish scale mucus, and sown in a successive-cropping garden for the netted melons; the soil is irrigated by nitrobacteria liquid once; caulicles and laminas of the netted melons are gradually inoculated with mycosphaerella, melon fusarium oxysporum, melon phytophthora, bacillus anthraci, sphaerotheca erysiphales and tobacco ringspot viruses stage by stage after two pieces of true netted melon leaves grow; Chinese alpine rush leach liquor, allium caelureum leach liquor, subprostrate sophora leach liquor, 5% mirabilite water solution and 0.2% neomycin sulfate water solution are timely sprayed after fungus or virus inoculation, and the healthy netted melons are kept; the treated netted melon seedlings have extremely high disease resistance, and high disease resistance and high yield can be achieved after the netted melon seedlings are sowed in successive cropping fields.