220 results about "Nonspecific adsorption" patented technology

It is an object of the present invention to provide a detection surface used for a biosensor wherein nonspecific adsorption is repressed. The present invention provides a biosensor comprising a substrate coated with a hydrophobic polymer.

It is an object of the present invention to provide a biosensor, which is not significantly affected by the baseline fluctuation and suppresses nonspecific adsorption. The present invention provides a biosensor, which comprises a metal surface or metal film coated with a hydrophobic polymer, and has two or more types of different surfaces in a region coated with a hydrophobic polymer.

It is an object of the present invention to provide a solid substrate used for sensors that suppresses nonspecific adsorption and that is able to immobilize a physiologically active substance. The present invention provides A solid substrate used for sensors, wherein two or more different hydrophobic polymer layers are laminated on the solid substrate, and among the above hydrophobic polymer layers, the surface of a layer, which is farthest from the solid substrate, is modified.

Provided is a magnetic particle holding carrier enabling automatization of treatment of a biological substance such as a protein by improving dispersibility of nano-size magnetic particles and suppressing nonspecific adsorption onto the wall of a container such as a pipette tip without damaging the properties of the nano-size magnetic particles such as a large solid-phase area and ability to arbitrarily design a functional protein, and provide a method for preparing the same. The magnetic particle holding carrier is formed of a micro-size nonmagnetic carrier and a plurality of nano-size magnetic particles bound to the carrier.

The invention relates to a separation and purification technique, in particular to an affinity chromatography stationary phase of immobilized metal and a preparation method thereof. The structure of the stationary phase is shown in the drawing; wherein, the particle size of GMAPolymer micro-sphere is 10nm-50um; the GMAPolymer micro-sphere is poly-methacrylic acid glycidyl esters micro-sphere. By carrying out amination and phosphoric esterification, a synthesis method of the high-performance poly-methacrylic acid glycidyl ester-group novel affinity chromatography stationary phase is gained and used for the research in phosphorylation proteomics and highly-selective separation, enrichment and purification of phosphopeptide by the chelation with zirconium ions and iron ions; meanwhile, compared with the traditional stationary phase, the affinity chromatography stationary phase reduces the nonspecific adsorption on the non-phosphopeptide.

A blood purification sorbent for antibody removal belongs to the technical field of biomedicine, which consists of the two parts of solid-phase carrier material and a petunidin fixed on the carrier by chemical coupling. The molecular structure of the petunidin is shown as above, wherein, one atom among A, B and C is N, the others are C; n is 0-2. The blood purification sorbent can adsorb the antibody component in plasma and autoantibodies such as rheumatoid factor, antinuclear antibody, etc. in heavy load, has limited nonspecific adsorption to other plasma components such as seralbumin, etc., and also has low preparation cost and stable physicochemical property. The material can be used as adsorption filler of a blood purification device for removing the autoantibody and immune complex in the plasma.

A target substance detection element that can effectively prevent the nonspecific adsorption of a target substance or impurities and detects the target substance with high sensitivity, a target substance detection kit, and a structure constituting the target substance detection element. The structure has a substrate, polymers present on the substrate surface, and first target substance capturing molecules bonded to the polymers. The polymer is composed of a polymer of a carboxybetaine monomer represented by General Formula (1) below. The first target substance capturing molecules are bonded to some of the carboxyl groups of the polymers. A compound represented by General Formula (2) is bonded to at least some of the carboxyl groups, from among the carboxyl groups of the polymers, that are not bonded to the first target substance capturing molecules.

The invention provides a zwitterionic polymer microsphere and a preparation method thereof. The zwitterion is introduced into the hydrophobic polymer microsphere through copolymerization to improve its hydrophilicity and reduce its non-specific binding. Introduce long-chain functional group molecules, and couple molecular probes through chemical bonds. On the one hand, the covalent combination makes the complex more stable. At the same time, the long-chain functional groups can reduce the steric hindrance during coupling and stabilize the activity of the bound biomolecules. The present invention also provides zwitterionic polymer magnetic microspheres and fluorescent microspheres and their preparation methods. In practical application, the present invention can well inhibit the non-specific adsorption of proteins. Balls are similar. It can effectively improve the stability of the reagent, reduce the background value of the reaction, and meet the requirements of the national standard or industry standard for the linear correlation, repeatability, recovery rate and other properties of the reagent. The invention has very wide application value of immunoassay.

The invention relates to a chip of a surface plasmon resonance instrument which is based on polyethylene glycol and a preparation method. The chip comprises a glass film base and a gold plating film on the glass film base; wherein the gold plating film is provided with an initiator layer which is introduced in the way of self-assembly and a functionalized polyethylene glycol layer which is combined with the initiator layer. The invention uses a quartz crystal microbalance to monitor the growth of the film at the surface of the chip at real time and the chip of the invention effectively solves the problem of non-specific absorption of protein. As the surface ornament of the chip is not limited to traditional carboxylic dextran, the density and types of the functional groups at the chip surface are no longer strictly restricted. Furthermore, the chip of the invention also has good performance in terms of the specificity maintenance and quality control of biological signaling molecule and so on. The preparation method of the chip of the invention has few steps and the low manufacturing cost and is more suitable for industrial production. The chip has more potential of deep development and more superior generality.

The invention belongs to the technical fields of polymer materials and analysis, relates to a monodispersed organic and inorganic hybrid microsphere particle with core-shell structure and preparation and application thereof. Inorganic silica particles as the core are modified by a silanizing agent, and then 3-acetylaminophenylboronic acid and polyene cross-linking monomer coat on the click nuclear surface through a mercaptan-alkene click chemistry technology to form organic and inorganic hybrid nano / micron particles with core-shell structure, smooth surface and boric acid functional groups. According o the invention, introduction of the phenyl boronic acid functional group to the material surface by a mercaptan-alkene method not only overcomes the shortcomings of complex procedure and low reaction efficiency in a post modification method but also allows stable polymerization of APBA, increases surface bonding amount of boric acid and hydrophilic property of the material, and reduces the nonspecific adsorption of proteins. The microsphere particles of the invention can be used in separation or enrichment of glycoprotein with 1,2-cis diol structure, and has good practical value and application prospect in the field of proteomics.

The present invention relates to preparation method of aflatoxin immuno-affinity column, including steps of: preparation of hydrazine antibody: employing sodium periodate oxidating unconjugated active area Fc hydroxoy of antibody to aldehyde group, and reacting with adipic dihydrazide to gain hydrazine antibody; preparation of aldehyde solid substrate: oxidating solid substrate containing continued diol structure by sodium periodate to gain aldehyde solid substrate; coupling of antibody and solid substrate: mixing hydrazine antibody and aldehyde solid substrate, adding sodium borohydride to seal unreacted aldehyde group on solid substrate after oscillating reaction to gain coupling antibody solid substrate; column packed. The present invention couples the unconjugated active area Fc end of antibody to solid substrate to remain activity of antibody better; hydrazide derived on antibody instead of solid substrate to avoid nonspecific adsorption of hydrazide group; the combined affinity column not introducing new groups ( such as carboxyl, amido and so on) except coupling antibody to avoid nonspecific adsorption from them.

The invention relates to the field of drug screening, in particular to a chip, a preparation method, an application and a method for screening drugs. The chip is capable of resisting protein nonspecific adsorption, parallel fixing multiple kinds of small molecular substances and being used for screening drugs with complicated components. Due to the adoption of the chip, bioactivity of the small molecular compound and a specific disease target can be effectively analyzed only through trace compound, and the sensitivity is high.

The invention discloses a preparation method for a small-molecule microarray. The preparation method comprises the steps of bonding the hydroxyl terminal of a nonspecific adsorption resistance material modified on a chip substrate with the hydroxyl terminal of a small-molecule light crosslinking agent through an esterification reaction, and randomly fixing small molecules on the hydroxyl terminals through light crosslinking. By the adoption of a direct esterification mode, the small-molecule light crosslinking agent is quickly and efficiently coupled to the surface of the nonspecific adsorption resistance material; a chip modification process is simple and convenient and can be implemented under a normal temperature condition; therefore, the modification cost is reduced, and the modification efficiency is improved; a high detection signal is guaranteed; a non-reacted group still keeps high nonspecific adsorption resistance capacity, so that a background signal is reduced; the prepared small-molecule microarray is high in detection signal and low in background signal and can be used for mutual action between high-flux screened small-molecule medicaments and targets.

The invention discloses a multi-index disease combined detection microfluidic device, which comprises a microfluidic reaction chip, a microfluidic storage chip, a direct current motor driving module,a reaction chip fluid control module, a storage release fluid control module, a temperature control module, and a CCD detection module. Through the cooperation between the reaction chip fluid controlmodule, the storage release fluid control module, the temperature control module, and the microfluidic reaction chip, a sample and a sample diluting solution are mixed; microsphere surface coated antibodies capture target cell specific antigens, non-specific adsorbed cell antigens on the microsphere surface are washed off, target cell antigens specifically absorbed on the microsphere surface capture enzyme labeled antibodies, exciting liquids (A and B) are mixed, enzymes specifically adsorbed on the microsphere surface catalyze the chemiluminescence of the exciting liquids; the CCD detection module detects and collects the chemiluminescence signals of the microsphere surface, and multiple index antigens in a serum sample are captured and detected automatically.

The invention discloses a method for hydrophilically modifying a polyacrylic ester or polyacrylic ester copolymer material and a product prepared with the method. The modification method comprises the following steps of: (1) performing swelling pretreatment on microspheres by using an organic solvent; (2) under the action of an alkaline catalyst, catalyzing ring opening of an epoxy group for reacting with hydroxy groups of polysaccharides to generate a stable ester bond, and chemically bonding hydrophilic polysaccharide molecules on the surfaces of the microspheres; and (3) under an alkaline condition, crosslinking and reinforcing the polysaccharide molecules bonded on the surfaces of the microspheres by using a bifunctional crosslinking agent. The method provided by the invention is easy and convenient to operate, and has mild reaction conditions; and an obtained hydrophilic material is stable, a coating is prevented from falling easily, and nonspecific adsorption of polymer microspheres on proteins is well suppressed. According to hydrophilically-modified polyacrylic ester microspheres, rich hydroxy groups contained in a hydrophilic layer can be further derived into other various functional groups; and the microspheres have great application prospects in the field of biotechnology, particularly in the field of biochemical separation.

The invention discloses a functional polymer thin film, a preparation method of the thin film and an application of the thin film. The preparation method comprises the following steps: (1) modifying a photo-cross-linking agent on the surface of a substrate; (2) rotationally coating the surface formed in the step (1) with a polymer solution; (3) performing irradiation reaction on the polymer solution with ultraviolet lights and covalently grafting the polymer to the substrate to form the polymer thin film; and (4) further functionalizing the polymer or directly performing biological sensing application. According to the preparation method, a three-dimensional polymer surface can be simply, quickly and efficiently prepared; the density and thickness of the surface polymer can be precisely controlled by regulating and controlling the density of the photo-cross-linking agent, the molecule weight of the polymer and spin-coating parameters; moreover, the preparation method is strong in operability, excellent in repeatability and suitable for mass production. The surface of the prepared polymer thin film has the high requirement for the fixed amount of bio-molecules and the excellent anti-nonspecific adsorption ability, thus the detection performance of a biological sensor (for example, surface plasmon resonance imaging, fluorescence, quartz crystal microbalance and electrochemical transducer) can be improved.

The invention discloses application of hyperbranched polyglycerol modified magnetic nanoparticle microspheres in chemiluminescence immune assay. By utilizing the hyperbranched polyglycerol modified magnetic nanoparticle microspheres, a nanometer microsphere material of which the surface is hydrophilic and which is enriched in functional groups is prepared. When a chemiluminescence diagnostic agent based on the hyperbranched polyglycerol modified magnetic nanoparticle microspheres is actually used, the reagent can well inhibit nonspecific adsorption of proteins, and the antibody coupling amount is 2-3 times higher than that of commercial magnetic microspheres. In the project-based performance analysis, the diagnostic agent based on the magnetic microspheres can greatly enhance the reaction between antigens and antibodies. When the reagent is compared with a commercial magnetic microsphere reagent, the sensitivity is improved by 4-5 times, and the upper detection limit is improved by 1.3 times, so that the detection range is improved by nearly 6.5 times.

An object of the present invention is to overcome the problem of nonspecific adsorption of a labeled antibody on the detection site and to provide an immunochromatography method by which the false positive signal can be suppressed, and wherein the detection sensitivity is high and the highly reliable measurement can be performed. The present invention provides an immunochromatography method, which comprises developing an analyte and a labeling substance modified with a first antibody against the analyte on a porous carrier in a state where the analyte and the labeling substance is mixed, and capturing the analyte and the labeling substance at the reaction site on the porous carrier having a second antibody against the analyte, so as to detect the analyte; wherein a fragmented antibody is used as the first antibody and / or the second antibody; an analyte is detected via amplification using an amplification solution which contains a compound containing silver and a reducing agent for silver ions; and the ratio of the non-specifically adsorbed labeling substance in the pre-amplification detection site and the non-detection site of the porous carrier is between 0.4 to 2.5 in a system where the density of the non-specifically adsorbed labeling substance is 106 / mm2 or less.

The present invention relates in general to the preparation and use of matrices having solid spaces, interstitial spaces and interstitial polymer networks. In particular, the interstitial polymer networks have utility in chemical and biochemical separations, solid phase synthesis, catalysis of chemical reactions, and immobilized enzyme reactors. The interstitial polymer networks in one embodiment comprise crosslinked polymers suspended in the interstitial spaces from and / or between solid particles. The matrices are characterized by high ligand and functional group density and by reversible high sorptive and binding capacity, and are substantially accompanied by a very low nonspecific adsorption or interaction with molecules such as proteins. Moreover, the matrices of the invention exhibit other characteristics highly desirable in chromatographic and catalytic applications, such as high physical rigidity, high ligand density, chemical stability, high ligand reactivity, and rapid exchange and reaction kinetics.

The invention relates to an analytical chromatographic method which comprises the steps of:a) providing a membrane type flow matrix attached to a liquid-impervious backing, which flow matrix permits a capillary force assisted lateral fluid flow therethrough, and at least a part of which flow matrix contains ion-exchange functions;b) treating the flow matrix to reduce or eliminate unspecific adsorption properties of the flow matrix;c) applying to the flow matrix a sample containing at least two components;d) initiating a first lateral flow of aqueous fluid to transport the sample through the flow matrix and separate said components therein;e) interrupting the lateral flow; and eitherf1) detecting at least one of the separated components on the flow matrix in the position reached by the respective component when the flow was interrupted; orf2a) initiating a second flow of aqueous fluid to transport the components in a direction substantially transverse to the direction of the first lateral flow;f2b) interrupting the second lateral flow; andf2c) detecting at least one of the separated components on the flow matrix in the position reached by respective component when the flow was stopped.The invention also relates to a chromatographic device comprising a membrane type flow matrix attached to a liquid-impervious backing, which membrane permits a capillary force assisted lateral fluid flow therethrough and at least a part of which is modified to support ion-exchange functions.

The present invention relates in general to the preparation and use of matrices having solid spaces, interstitial spaces and interstitial polymer networks. In particular, the interstitial polymer networks have utility in chemical and biochemical separations, solid phase synthesis, catalysis of chemical reactions, and immobilized enzyme reactors. The interstitial polymer networks in one embodiment comprise crosslinked polymers suspended in the interstitial spaces from and / or between solid particles. The matrices are characterized by high ligand and functional group density and by reversible high sorptive and binding capacity, and are substantially accompanied by a very low nonspecific adsorption or interaction with molecules such as proteins. Moreover, the matrices of the invention exhibit other characteristics highly desirable in chromatographic and catalytic applications, such as high physical rigidity, high ligand density, chemical stability, high ligand reactivity, and rapid exchange and reaction kinetics.

According to the present invention, there can be provided a structure preventing the nonspecific adsorption, and a target substance-detecting element and a target substance-detecting kit. A first aspect of the present invention is a structure including: a substrate; and a membrane, present on the surface of the substrate, having a polymer of a monomer represented by the following General Formula (1) or (2):(wherein R represents H or CH3; x represents an integer of 2 to 5, and y represents an integer of 1 to 7, with the proviso that x+y=an integer of 3 to 10), and(wherein R1=H or CH3, and R2 represents O or NH; a represents an integer of 2 to 5, and b represents an integer of 1 to 7, with the proviso that a+b=an integer of 3 to 10.)

The invention discloses an in-vitro diagnostic check test strip and a manufacturing method thereof. The in-vitro diagnostic check test strip comprises a substrate, a reaction membrane and a mat, wherein, the substrate is adhered with the reaction membrane and the mat, one end of the reaction membrane is mutually connected with the mat in a lapping joint way, the substrate is made of transparent plastic or PVC material, the surface of the substrate, which is adhered with the reaction membrane, is provided with parallel checking line and quality-control line, and the mat is coated with colloidal gold solution or colored latex solution with the diameter range of 10nm to 50nm; the manufacturing method comprises the steps of preparation of the reaction membrane, marking and solid phase of the mat, assembly and cutting. The invention has the advantages of being capable of judging the testing result directly by the transparent substrate, preventing the volatilization of liquid and the infiltration of takeout liquid, causing the testing result to be more accurate, reducing nonspecific adsorption during testing reaction, reducing reaction background, preventing tracer from being adsorbed on the reaction membrane, guaranteeing fast elution and release, and being simple and convenient and feasible.

The invention discloses a zwitterion-containing water-soluble cross-linking agent as well as a preparation method and application thereof. The structural formula of the compound is a formula I. The compound has good water solubility and anti-nonspecific adsorption feature, so that the compound can be used as a cross-linking agent to prepare the polymer with high cross-linking degree, the obtained polymer has excellent biological pollution resistant feature, and can be widely applied to the fields such as biomedicine and materials. The water-soluble cross-linking agent is capable of reducing the protein aggregation speed, so that the protein molecule can be more easily presented in crystal form, and therefore the water-soluble cross-linking agent can be used for synthesizing the biomaterial for promoting protein crystallization. Furthermore, the cross-linking agent molecule comprises two-NH-C=O groups capable of generating hydrogen bond interaction with the carbohydrate (-OH) abound on the surface of microorganism; therefore, the cross-linking agent molecule can be used as an additive to prepare a molecular imprinting film material so as to selectively separate the microorganism (structural formula).

The invention relates to a preparation method of a yeast microsphere magnetic composite material surface imprinted adsorbent. The preparation method is characterized by taking a yeast microsphere magnetic composite material as a matrix material, pyrethroid pesticide as template molecules, alpha-methacrylic acid (MAA) as functional monomers, ethylene glycol dimethacrylate (EGDMA) as a cross-linking agent and 2,2'-azobisisobutyronitrile (AIBN) as an initiator to prepare the yeast microsphere magnetic composite material surface imprinted adsorbent through a suspension polymerization process. The preparation method has the advantages that the template molecules are thoroughly removed, and the compatibility with an imprinted high polymer molecular layer is good. The prepared magnetic composite material surface imprinted adsorbent can be used for selectively identifying and separating pyrethroid pesticide in environmental water, and the problem that partial template molecules cannot be eluted due to too-deep embedding is avoided, and the obtained imprinted adsorbent is high in mechanical strength, identification points are not easily damaged, and nonspecific adsorption is great reduced.

The invention provides a magnetic hyperbranched polymer or magnetic hyperbranched polymer derivative blood heavy metal ion adsorbent and a preparation method thereof. The preparation method includes: 1) modifying the surfaces of magnetic ferroferric oxide nanoparticles with polydopamine layers to obtain magnetic ferroferric oxide-polydopamine; and 2) mixing the obtained magnetic ferroferric oxide-polydopamine with a cross-linking agent in a solvent for a reaction, adjusting pH of the solution to 7.5-9, adding hyperbranched polymer, and carrying out a reaction while performing stirring to prepare the magnetic hyperbranched polymer or magnetic hyperbranched polymer derivative blood heavy metal ion adsorbent. The adsorbent can effectively capture trace heavy metal ion in blood, is high in biocompatibility, can selectively adsorb heavy metal ions, and prevents nonspecific adsorption of other elements in blood and the adsorbent itself from affecting blood components. The method is simple in process, mild in synthetic condition, short in production cycle, and suitable for industrial production and has bright application prospects in the medical field.

The invention relates to a water soluble cationic polymer adsorption material in the biomedical material field and the application thereof, which contains the quaterisation reaction synthesis of natural polysaccharides with amino groups and hydroxyls and the derivatives thereof or chemosynthetic polymers. The invention is characterized in that the molecule of the polymer adsorption material has a structure in formula I: in the formula, R is selected from the natural polysaccharides with amino groups and hydroxyls and the derivatives thereof or the chemosynthetic polymers, the molecular weight of which is from 6 KD to 500 KD; X is selected from a C0 to C6 paraffinic chain with O atoms, N atoms or OH groups; R1, R2 and R3 are selected from a C1 to C4 alkyl group. After the adsorption material is dissolved in a medical dialysis solution, the material can be applied to a blood purification treatment for severe hepatitis patients. The invention has the advantages of removing protein-bound toxin and water soluble toxin molecule at the same time, low cost which is only about one tenth of that of a molecular adsorbent recirculating system and little nonspecific adsorption to plasma protein.

The invention relates to an enzyme stabilizer comprising a lignin derivative produced by reaction between lignin and a hydrophilic compound, and to a method of saccharifying lignocellulosic biomass which employs the enzyme stabilizer. According to the invention it is possible to accomplish effective saccharification of cellulosic biomass with saccharifying enzymes, by enhancing saccharifying enzyme activity and preventing nonspecific adsorption of saccharifying enzyme onto substrate.

The invention provides an anti-bioadhesive material, which is a copolymer formed by copolymerization of a zwitterionic monomer and a phosphonic acid or phosphonate monomer. On the other hand, the invention also provides a preparation method of the anti-bioadhesive material. The method includes: directly mixing the zwitterionic monomer with the phosphonic acid or phosphonate monomer for copolymerization, or firstly preparing a homopolymer of one of the two monomers, then adopting the prepared homopolymer as the macroinitiator or chain transfer agent, and taking the other monomer of the two monomers as the polymerization monomer to prepare a copolymer of the zwitterionic monomer and the phosphonic acid or phosphonate monomer. The invention also provides an anti-bioadhesive metal instrument,the surface of which is provided with a coating of the anti-bioadhesive material. The anti-bioadhesive material has high stability, and by means of a simple coating process, a stable polymer coating can form on the metal surface, an anti-bioadhesive material surface modified metal surface protein has significantly reduced nonspecific adsorption performance, and the anti-bioadhesive performance issignificantly improved.