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Zwitterionic polymer microspheres and preparing method thereof

A zwitterionic, hydrophobic polymer technology, applied in the field of bioseparation and biomedical engineering, to achieve the effect of reducing the background value, superior reagent performance, and broad application value of immunoassay

Inactive Publication Date: 2016-06-22
于乐
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The introduction of zwitterions into hydrophobic polymer microspheres by copolymerization to improve their hydrophilicity and reduce their non-specific binding has not been reported in the current art

Method used

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  • Zwitterionic polymer microspheres and preparing method thereof
  • Zwitterionic polymer microspheres and preparing method thereof
  • Zwitterionic polymer microspheres and preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Preparation of phosphoric acid type zwitterionic polystyrene microspheres

[0051] Add 45 mg of sodium dodecylsulfonate to a four-necked flask equipped with a condenser tube (the top of the condenser tube needs to be connected to a drying tube), nitrogen passage, magnetic stirring, and water bath heating, and add 100 mL of purified water and stir to dissolve. Then pass nitrogen to remove oxygen, and the flow rate of the air flow is moderate. It is advisable to introduce nitrogen into the medium so that there are continuous small bubbles coming out, and the air flow is uninterrupted. During nitrogen flow, use a pipette to measure 4mL of purified styrene, and slowly add it dropwise through the feeding port of the four-neck flask, weigh 36mg of potassium persulfate, dissolve it in 1mL of purified water, and add it to the reaction system. The temperature was set to 85° C., and the polymerization was initiated by heating. Weigh 10 mg of 2-methacryloyloxyethyl phos...

Embodiment 2

[0056] Example 2 Preparation of Carboxylic Acid Type Zwitterionic Polystyrene Microspheres

[0057] Preparation method is with reference to embodiment 1, and specific implementation method is as follows:

[0058] Add 45 mg of sodium dodecylsulfonate to a four-necked flask, add 100 mL of purified water and stir to dissolve. Then, oxygen was blown with nitrogen gas. During the nitrogen gas flow, 4 mL of styrene was measured with a pipette, and slowly added dropwise through the feeding port of the four-neck flask. Weigh 36mg of potassium persulfate, dissolve it with 1mL of purified water, add it to the reaction system, set the temperature of the water bath to 85°C, and heat to initiate polymerization. Weigh 10 mg of N,N-dimethyl-N-methacryloyloxyethyl-N-acetic acid inner salt, dissolve it with 1 mL of long-chain carboxyl monomer and stir until it becomes milky white, and keep nitrogen gas flowing during this process. The milky white liquid was added to the styrene reaction syst...

Embodiment 3

[0060] Example 3 Preparation of phosphoric acid type zwitterionic polystyrene magnetic microspheres

[0061]Add 130ml of water and 20ml of ethanol into a four-necked flask equipped with a condenser tube, nitrogen passage, ultrasonic vibration rod and mechanical stirring, and mix well, then add 90mg of sodium dodecylsulfonate and 270mg of cetyl alcohol, and heat to 50°C. Dissolve it and pass nitrogen gas to remove oxygen. Set the stirring blade speed to 500rpm. During nitrogen flow, pipette 10ml styrene with a pipette and add it to 0.4g oleic acid-modified ferric oxide. Ultrasonic dispersion is uniform and then slowly added dropwise into the four-necked flask reaction system. Add 36 mg of potassium persulfate aqueous solution, and turn on the ultrasound (power 650W, frequency 30%, working for 2 seconds, intermittent for 1 second), to disperse the oil-soluble monomer and magnetic particles evenly to form a uniform gray reaction system, and finally add positive ten Hexane 2ml to...

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Abstract

The invention provides a zwitterionic polymer microsphere and a preparation method thereof. The zwitterion is introduced into the hydrophobic polymer microsphere through copolymerization to improve its hydrophilicity and reduce its non-specific binding. Introduce long-chain functional group molecules, and couple molecular probes through chemical bonds. On the one hand, the covalent combination makes the complex more stable. At the same time, the long-chain functional groups can reduce the steric hindrance during coupling and stabilize the activity of the bound biomolecules. The present invention also provides zwitterionic polymer magnetic microspheres and fluorescent microspheres and their preparation methods. In practical application, the present invention can well inhibit the non-specific adsorption of proteins. Balls are similar. It can effectively improve the stability of the reagent, reduce the background value of the reaction, and meet the requirements of the national standard or industry standard for the linear correlation, repeatability, recovery rate and other properties of the reagent. The invention has very wide application value of immunoassay.

Description

technical field [0001] The invention belongs to the fields of biological separation and biomedical engineering, and in particular relates to a zwitterionic polymer microsphere and a preparation method thereof. Background technique [0002] Immunoassay technology is a biochemical detection method based on the specific interaction of antigens and antibodies. Its development originated from the immunoturbidimetric detection technology invented in the 1850s. Immunoturbidimetric detection can use the precipitation or turbidity formed after the antigen-antibody reaction for direct determination, but in clinical testing, the content of some analytes in specimens is very low (even at nanogram levels). Therefore, in order to find a method to increase the sensitivity, the antigen or antibody in the detection reagent is labeled with a substance that can be measured in a small amount, and the sensitivity is improved by measuring the marker. The emerging labeling immunoassay techniques ...

Claims

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Application Information

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IPC IPC(8): C08F212/08C08F230/02C08F222/26C08F220/34C08F220/38C08F257/02C09K11/06G01N33/533G01N33/558
Inventor 不公告发明人
Owner 于乐
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