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Functional polymer thin film, preparation method and application thereof

A functional polymer and polymer technology, which is used in the testing of pharmaceutical preparations, material inspection products, and biological testing. Application prospects, fixation and detection signal enhancement effects

Active Publication Date: 2015-05-06
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the reactive functional groups of polymers are connected by chemical bonds. Different reaction systems should be used for different functional groups, and there are relatively many considerations. Moreover, for liquid-solid interface reactions, the reaction rate is usually not high, the time is long, and it is difficult for the polymer to move in the solution. The steric hindrance of its own space is large, resulting in limited local reactions and more prominent shortcomings

Method used

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  • Functional polymer thin film, preparation method and application thereof
  • Functional polymer thin film, preparation method and application thereof
  • Functional polymer thin film, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1. Dextran surface (small molecule detection: flow-through immobilized SA, detection of Biotin)

[0049] (1) Prepare a layer of chromium layer with a thickness of 3nm and a layer of gold with a thickness of 47nm on the glass substrate by thermal evaporation method, as the substrate of the biochip.

[0050] (2) Prepare the ethanol solution HS-(CH 2 ) 11 -EG6-OH at a concentration of 1 mM.

[0051] (3) The substrate of the biochip is cleaned with ethanol or deionized water, and then the biochip is placed in a plasma cleaner for cleaning for 3 minutes.

[0052] (4) Soak the biochip in the prepared thiol solution and incubate at 4°C for 12 hours. After the predetermined time, the biochip is taken out, washed with ethanol and deionized water alternately, and dried with nitrogen.

[0053] (5) Prepare 20 mL of the solution required for esterification and linking photocrosslinking agent, 10 mM carboxy-terminal photocrosslinking agent, 1-(3-dimethylaminopropyl)-3-ethy...

Embodiment 2

[0065] Example 2. Poly(polyethylene glycol methacrylate) surfaces (small molecule arrays)

[0066] (1) Prepare a layer of chromium layer with a thickness of 3nm and a layer of gold with a thickness of 47nm on the glass substrate by thermal evaporation method, as the substrate of the biochip.

[0067] (2) Preparation of ethanol solution of hydroxyl-terminated and carboxyl-terminated thiols (HS-(CH 2 ) 11 -EG6-OH and HS-(CH 2 ) 11 -EG6-COOH), the concentration is 1mM, the above two thiol solutions are mixed according to 999:1 (v / v), set aside.

[0068] (3) Clean the biochip with ethanol or deionized water, and then put the biochip into a plasma cleaner for cleaning for 3 minutes.

[0069] (4) Soak the biochip in the mixed thiol solution and incubate at 4°C for 12 hours. After the predetermined time, the biochip is taken out, washed with ethanol and deionized water alternately, and dried with nitrogen.

[0070] (5) Activate the carboxyl groups on the surface of the biochip, ...

Embodiment 3

[0087] Embodiment 3. Dextran surface (antigen antibody fluorescent detection)

[0088] (1) The glass substrate is cleaned with ethanol or deionized water, and the biochip is cleaned in a plasma cleaner for 3 minutes to be used as the substrate of the biochip.

[0089] (2) Prepare 3-aminopropyltriethoxysilane (APES) diluted with acetone at 1:50, take it out after 20-30s, and then use pure acetone solution to remove unbound APES, so that APES can be combined with glass, A monolayer is formed on the surface.

[0090] (3) Soak in 25% glutaraldehyde for 30 minutes, wash with acetone, immerse in 1 mM aqueous solution of PEG with one end being an amino group and one end being a hydroxyl group, and incubate at room temperature for 1 h. After the predetermined time is reached, the biochip is taken out and used Rinse with deionized water and dry with nitrogen gas.

[0091] (4) Prepare 20 mL of the solution required for esterification and linking photocrosslinking agent, 10 mM carboxy-...

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Abstract

The invention discloses a functional polymer thin film, a preparation method of the thin film and an application of the thin film. The preparation method comprises the following steps: (1) modifying a photo-cross-linking agent on the surface of a substrate; (2) rotationally coating the surface formed in the step (1) with a polymer solution; (3) performing irradiation reaction on the polymer solution with ultraviolet lights and covalently grafting the polymer to the substrate to form the polymer thin film; and (4) further functionalizing the polymer or directly performing biological sensing application. According to the preparation method, a three-dimensional polymer surface can be simply, quickly and efficiently prepared; the density and thickness of the surface polymer can be precisely controlled by regulating and controlling the density of the photo-cross-linking agent, the molecule weight of the polymer and spin-coating parameters; moreover, the preparation method is strong in operability, excellent in repeatability and suitable for mass production. The surface of the prepared polymer thin film has the high requirement for the fixed amount of bio-molecules and the excellent anti-nonspecific adsorption ability, thus the detection performance of a biological sensor (for example, surface plasmon resonance imaging, fluorescence, quartz crystal microbalance and electrochemical transducer) can be improved.

Description

technical field [0001] The invention relates to a simple, rapid and universal polymer film, a preparation method and its application in biosensing, especially in surface plasmon resonance imaging. Background technique [0002] Biochips mainly immobilize biological molecules such as proteins, peptides, and nucleic acids, as well as biological samples such as cells and tissues, on the surface of the chip by chemically modifying the surface of the substrate, thereby achieving accurate, rapid, and large-information detection of target biological components. Features such as high parallelism, diversity, miniaturization and automation. Reasonable surface chemical modification can make the immobilization of biological samples more efficient and stable. In order to improve the immobilization ability of biochips on biological samples, reduce non-specific adsorption, increase the signal intensity of detection, and then improve the detection sensitivity of instruments, a lot of resear...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48G01N33/15C03C17/38
Inventor 朱劲松周文菲杨墨李少鹏王瑞
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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