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Blood-purifying adsorbing agent for cleaning antibody

A blood purification and adsorbent technology, applied in the field of biomedicine, can solve the problems of difficult storage and disinfection, poor selectivity of pathogenic factors, limited application range, etc., and achieve low production cost, high antibody adsorption selectivity, and good stability Effect

Inactive Publication Date: 2008-10-08
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the existing problems in the prior art: (1) the protein A adsorbent is expensive, difficult to store and sterilize, and has a limited scope of application; (2) the adsorbent with hydrophobic amino acid as ligand has poor selectivity to pathogenic factors, Defects such as insignificant treatment effect

Method used

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  • Blood-purifying adsorbing agent for cleaning antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Coupling of 4-Mercaptoethylpyridine (4-MEP) on agarose gel with alkenyl reactive groups (activated by bromopropene)

[0019] 10g Sepharose CL-6B agarose gel was washed with 200mL deionized water several times and then uniformly dispersed in 5mL 3M NaOH solution, 2mL bromopropene was mixed with the gel suspension, and the resulting mixture was suspended and stirred at 30°C for 18 hours. After the reaction, the gel was washed with 100 mL of anhydrous acetone and 100 mL of deionized water several times. The agarose gel with alkenyl active groups activated by bromine is obtained.

[0020] Weigh 1g of 4-MEP·HCl (hydrochloride salt form), dissolve it in 1mL of deionized water, adjust the pH to 7 with 10M NaOH, and then add 6mL of phosphate buffer (0.1M, pH7) and 3mL of acetonitrile. The activated agarose gel was added to the mixing system and stirred at 30°C for 12 hours. After the reaction, the gel was washed with 100 mL of acetonitrile, 100 mL of deionized water, and...

Embodiment 2

[0022]Example 2: Coupling of 4-MEP on agarose gel (activated by divinyl sulfone) with alkenyl reactive groups

[0023] Take 10g of agarose gel substituted with primary amino groups and evenly disperse it in 60mL of a mixed solution composed of 0.1M carbonic acid buffer, pH9.5 and ethanol (35 / 65, v / v), and add 5mL to the reaction system. Vinyl sulfone was stirred with a paddle at 45°C for 20 hours. The gel suspension was washed with ethanol and then dispersed in 30 mL of a mixed solution composed of 0.05M carbonate buffer, pH 8.6 and ethanol (35 / 65, v / v). Weigh 1 g of 4-MEP·HCl, dissolve in 1 mL of deionized water, adjust the pH to 8.6, and add to the reaction system. Stir with a paddle at 45°C for 24 hours. After the reaction, the gel was thoroughly washed with ethanol, and then dispersed in 60 mL of 1M ethanolamine aqueous solution (pH 10.5), and stirred at 45°C for 2.5 hours. After the reaction, the gel was washed several times with 200 mL of deionized water.

[0024] The evalua...

Embodiment 3

[0025] Example 3: Coupling of 3-Mercaptoethylpyridine (3-MEP) on agarose gel with alkenyl reactive groups (activated by bromopropene)

[0026] The adsorbent synthesis method and evaluation method are the same as in Example 1, except that the 4-MEP·HCl used in the reaction is replaced with 3-MEP·HCl. The evaluation results show that the binding capacity of the adsorbent to adsorb immunoglobulin from human serum is 23.2 mg / ml, and the purity of the immunoglobulin in the eluate is greater than 85%.

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Abstract

A blood purification sorbent for antibody removal belongs to the technical field of biomedicine, which consists of the two parts of solid-phase carrier material and a petunidin fixed on the carrier by chemical coupling. The molecular structure of the petunidin is shown as above, wherein, one atom among A, B and C is N, the others are C; n is 0-2. The blood purification sorbent can adsorb the antibody component in plasma and autoantibodies such as rheumatoid factor, antinuclear antibody, etc. in heavy load, has limited nonspecific adsorption to other plasma components such as seralbumin, etc., and also has low preparation cost and stable physicochemical property. The material can be used as adsorption filler of a blood purification device for removing the autoantibody and immune complex in the plasma.

Description

Technical field [0001] The invention relates to a blood purification adsorbent for antibody elimination, belonging to the technical field of biomedicine. Background technique [0002] Autoantibodies and immune complexes have been proved to be closely related to the production and development of a series of diseases, such as immune response (allergies, transplant rejection), autoimmune diseases (systemic lupus erythematosus, rheumatoid, etc.), and even cancer. Therefore, there is a clinical need for an adsorbent that can selectively remove autoantibodies and circulating immune complexes from human blood in order to relieve acute symptoms, curb the development of the disease and play a therapeutic effect on the disease. As the incidence of such diseases, especially autoimmune diseases, increases year by year, this demand is also increasing. Based on the above considerations, many institutions have developed adsorbent materials with different properties, some of which have been appl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/22A61M1/38
CPCB01J20/3248B01J20/3208A61M1/3679B01J20/3246B01J20/3242B01J20/3217B01J20/3255
Inventor 贾凌云任军张丹妮林雪邳志前谢健
Owner DALIAN UNIV OF TECH
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