The invention relates to a
silica gel matrix separation material, wherein two or more
silane reagents form nonpolar / polar copolymerization
stationary phase through the copolymerization on
silica gel surface. The
structural formula of the nonpolar / polar copolymerization
stationary phase is as follows: FORMULA, wherein
Silica Gel is
silica gel; NP stands for nonpolar group, which is one or more of C1 to C30 normal chain
alkyl and phenyl; and P stands for polar group, which is one or more kinds of C1 to C12 normal chain
alkyl whose
tail end has the functional group such as
chlorine atom, bromineatom, cyan, amido,
benzenesulfonic acid group, sulfo group, carboxyl,
quaternary ammonium group and
alcohol group. The bonded phase of the separation material has novel structure, and simultaneously has nonpolar group and polar group, thereby being capable of providing hydrophobic force and polymorphic polar acting force, and greatly improving the selectivity of the inverse and efficient liquid chromatogram. The
stationary phase prepared with the preparation method provided by the invention has the advantages of even and stable
surface bonding group, large bonding quantity, and the like.