64results about How to "High bonding capacity" patented technology

The invention relates to a silica gel matrix separation material, wherein two or more silane reagents form nonpolar / polar copolymerization stationary phase through the copolymerization on silica gel surface. The structural formula of the nonpolar / polar copolymerization stationary phase is as follows: FORMULA, wherein Silica Gel is silica gel; NP stands for nonpolar group, which is one or more of C1 to C30 normal chain alkyl and phenyl; and P stands for polar group, which is one or more kinds of C1 to C12 normal chain alkyl whose tail end has the functional group such as chlorine atom, bromineatom, cyan, amido, benzenesulfonic acid group, sulfo group, carboxyl, quaternary ammonium group and alcohol group. The bonded phase of the separation material has novel structure, and simultaneously has nonpolar group and polar group, thereby being capable of providing hydrophobic force and polymorphic polar acting force, and greatly improving the selectivity of the inverse and efficient liquid chromatogram. The stationary phase prepared with the preparation method provided by the invention has the advantages of even and stable surface bonding group, large bonding quantity, and the like.

This invention has discloses a green synthesis method for carbon quantum dot bonded silica gel chromatography filler, which comprises the concrete steps of: mixing the solid powder of aconitic acid and tryptophan to be ground evenly, letting the mixture react under the temperature of 180oC- 200oC for 3 - 6 hours. Through dissolution, centrifugation and freeze drying of the generated solid, the amino-functionalized carbon quantum dots (NCDs) are obtained; After that, NCDs are dispersed in DES and added with gamma-glycidoxypropyl trimethoxy siloxane(GPTMS). Under the protection of N2 and the temperature of 55oC- 65oC, the mixture is stirred to react for 22 - 25 hours, then the silica gel (SiO2) is added to react for another 12 - 48 hours. Water and absolute ethyl alcohol are alternatively used to wash the raw product generated from the reaction. Silica gel chromatography filler modified by carbon quantum dots will be generated after drying. This green synthesis method realizes the surface bond of carbon quantum dots and silica gel through epoxy group and amino. DES used in the process of reaction is reaction solvent, which serves as an excellent dispersing agent for carbon quantum dots and silica gel, ensures higher bonded amount, and at the same time, meets the requirements of green chemistry.

The present invention relates to a protein A immunoadsorption material for purifying blood and its preparation method. Said protein A immunoadsorption material is a high-molecular material which is obtained by using agarose gel as carrier matrix, making said agarose gel be reacted with diglycidyl ether coupling reagent to obtain active carrier, then making said active carrier and protein A implement coupling reaction. Said invention can be used for making clinical immunoadsorption therapy.

A process for preparing the chromatographic bonded immobile phase includes preparing gamma-epoxypropoxypropyl silica gel as precursor, reaction of sodium hydride calixarene in anhyrous toluene at 70-80 deg.C to obtain calixarene phenol sodium salt, carrying it on said precursor under the action of quaternary ammonium salt, and reaction in nitrogen or argon gas at 110-120 deg.c. Its advantages aresimple process, low cost and high acid stability.

The invention discloses a tetraazacalix [2] arene [2] triazine bonded silica gel solid phase extraction material, a preparation method and application thereof. The tetraazacalix [2] arene [2] triazine bonded silica gel solid phase extraction material has the following structural formula described in the specification. The preparation method comprises the steps of: firstly modifying silica gel by using aminopropyltriethoxysilane to prepare amino silica gel; and then reacting the amino silica gel react with tetraazacalix [2] arene [2] triazine to prepare the tetraazacalix [2] arene [2] triazine bonded silica gel solid phase extraction material. The tetraazacalix [2] arene [2] triazine bonded silica gel solid phase extraction material synthesized by adopting the preparation method has the characteristics of high bonding quantity, stable bonding layer, wide application range and the like. The filler can be used for simultaneously extracting organic matters and divalent inorganic copper ions from water, and has a recycling rate of polycyclic aromatic hydrocarbon and Cu<2+> being 90 percent and enriching multiple reaching 500 times. The tetraazacalix [2] arene [2] triazine bonded silica gel solid phase extraction material can be repeatedly used for multiple times, and has a good popularization and application prospect.

The invention discloses an embedded triazine ring amide silica gel stationary phase for liquid chromatograph and a preparation method thereof, wherein the embedded triazine ring amide silica gel stationary phase has high bonding quantity and stable bonding layer and contains two polar functional groups of triazine ring and amide. The structural formula of the obtained stationary phase is shown in the specification.

The invention discloses a preparation method of novel solid phase microextraction fiber based on a nucleic acid aptamer / nanogold / porous polymer coating. The method comprises the steps of washing quartz fiber with acetone, benzene, alkali and acid, and carrying out high-temperature activation and silanization treatment on the washed quartz fiber; feeding functional monomer, a cross-linking agent and an initiating agent into a polymerization solvent, then feeding the silanized quartz fiber, carrying out thermal initiation copolymerization reaction, and pulling out fiber after polymerization; carrying out silanization treatment on the prepared coating with a sulfydryl silanization reagent to obtain the quartz fiber with a sulphur modified porous polymer coating; putting the obtained quartz fiber into the prepared nanogold solution for bonding to obtain quartz fiber with a nanogold / porous polymer coating; putting the obtained quartz fiber into aptamer phosphate buffer solution, and bonding the quartz fiber with the nanogold / porous polymer coating and the aptamer for a certain period of time to obtain a solid phase microextraction head with the single-stranded DNA nucleic acid aptamer / nanogold / porous polymer coating.

The invention discloses a tetraoxacalix[2]arene[2]triazine bonded silica stationary phase with large bonding quantity and a stable bonding layer. The concrete preparation method comprises the following steps: in the presence of anhydrous potassium carbonate, 3-aminopropyltriethoxysilane silica reacts with the tetraoxacalix[2]arene[2]triazine in N2 at the temperature of 30-90 DEG C to obtain the tetraoxacalix[2]arene[2]triazine bonded silica stationary phase. Experimental result indicates that the calixarene bonded stationary phase synthesized by the method has the characteristics of relatively large bonding quantity, stable bonding layer and the like; and the method is easy, the preparation cost is low, and the preparation method is widely applicable. The tetraoxacalix[2]arene[2]triazine bonded silica stationary phase not only has the traditional ODS (octadecylsilane bonded silica) reversed-phase chromatography performance, but also can provide sites for various actions such as complexing, hydrogen bonding, pi-pi interaction, space matching and the like. Therefore, the tetraoxacalix[2]arene[2]triazine bonded silica stationary phase can replace ODS to some degree, and provide possibility in separating substances difficult to separate at the same time.

The invention discloses a 25,27-di(3-methyl-thio-ethoxy thiadiazole) calix[4]arene stationary phase which is low in cost, high in content of compounded calixarene bonded stationary phase bonded amount, and stable in stability bonding layer, and is prepared by the following steps of: under the action of phase transfer catalyst ammonium tetrabutyl bromide, carrying out nitrogen protection to gamma-glycidol ether bonded silica gel and calixarene derivative sodium phenolate, and preparing the object calixarene stationary phase at 80 DEG C. The calixarene stationary phase compounded by the method in the invention is high in bonded amount, stable in boned layer, simple and easy in compounding method, low in cost, free of pollution in the compounding process and the like. The stationary phase not only has traditional ODS (Octadecylsilyl) reversion phase chromatography performance, and has containing complexing action, pi-pi action, hydrogen bonding action and dipole-dipole action because of many action sites; in a certain degree, the stationary phase in the invention has special separation performance comparing with the traditional ODS column.

The invention relates to a preparation method of an organic-inorganic hybrid cyclodextrin chiral separation porous monolithic material. The preparation method comprises the following steps that an alkenylsilane coupling agent and an alkoxysilane coupling agent are mixed and are subjected to prehydrolysis; and the hydrolysate is added with alkenyl-containing cyclodextrin or a derivative of the alkenyl-containing cyclodextrin and an organic polymerization reaction initiator so that the alkenylsilane coupling agent and the alkoxysilane coupling agent undergo a polycondensation reaction in situ and the derivative of the alkenyl-containing cyclodextrin and the alkenylsilane coupling agent undergo a radical polymerization reaction in situ, and thus the organic-inorganic hybrid cyclodextrin chiral separation porous monolithic material with specific cyclodextrin derivative groups is obtained. The preparation method provided by the invention has simple processes, allows mild reaction conditions, can adopts different alkenyl-containing cyclodextrin derivatives according to different requirements, and realizes preparation of the organic-inorganic hybrid cyclodextrin chiral separation porous monolithic material.

The invention discloses a preparation method for a solid-phase micro extraction fiber extraction head. The preparation method comprises the following steps of: firstly, carrying out silane treatment on a bare quartz fiber; secondly, adding an acrylamide function monomer, a cross-linking agent and an initiator in a polymerization solution and fully mixing to obtain a prepolymerization solution; thirdly, coating a porous polymer coating on the surface of the quartz fiber subjected to silane treatment; fourthly, carrying out ageing treatment on the quartz fiber; fifthly, repeating the second step to the fourth step for multiple times and repeatedly coating the porous polymer coating on the surface of the quartz fiber for multiple times; sixthly, carrying out bonding reaction on the porous polymer coating and the quartz fiber; and seventhly, adding the quartz fiber treated in the sixth step into an aptamer solution and carrying out bonding reaction at room temperature. According to the preparation method disclosed by the invention, the quartz fiber is treated by the steps, and thus the bonded amount of the aptamer on the surface of the fiber can be remarkably improved; and an SPME (Solid-Phase Micro Extraction) head with a novel aptamer / porous polymer coating can be prepared.

The invention belongs to the technical field of chromatographic separation and particularly relates to a bonded silica gel stationary phase with multi-acting-force mixed modes as well as a preparation method and application thereof. The bonded silica gel stationary phase is an ionic liquid bonded silica gel stationary phase containing aryl and fatty group at the same time. The stationary phase provided by the invention is stable in bonding layer, has separation characteristics of the multi-acting-force multiple modes, and wide application prospect. The preparation method of the bonded silica gel stationary phase is simple, lower in preparation cost and wider in applicable range.

The invention discloses a method for forming a die bonding connection structure of a reflective LED at low temperature, which comprises the following steps of: forming a first metal layer on a first surface of a base plate, and providing an LED epitaxial structure on a second surface of the base plate; forming a second metal layer on a base body, wherein the material of the second metal layer is different from that of the first metal layer; applying a force to the base plate and the base body so that the first metal layer and the second metal layer produce plastic deformation and are primarily bonded together; and heating the base plate and the base body in a high temperature furnace, wherein the first metal layer and the second metal layer are subjected to solid state diffusion in an interface to form a diffusion alloy layer.

The invention discloses nucleic acid aptamer-molecular imprinting synergistic recognition magnetic microspheres and a preparation method and application thereof. The nucleic acid aptamer-molecular imprinting synergistic recognition magnetic microspheres disclosed by the invention comprise magnetic microsphere inner cores and nucleic acid aptamer-molecular imprinting synergistic recognition shells;the nucleic acid aptamer-molecular imprinting synergistic recognition shells have molecular imprinting polymer rigid recognition cavities; the molecular imprinting polymer rigid recognition cavitieshave structural stability, difficult deformation and strong specific adsorptivity; and nucleic acid aptamers are not easy to digest.

The invention relates to the technical field of polysaccharide-based chiral stationary phases, and discloses a method for preparing a chiral stationary phase of triazolyl bonding amylose and a derivative thereof from silica gel through a click reaction. The chiral stationary phase of bonding-type amylose and the derivative thereof is prepared by conducting selective modification on an amylose 6-hydroxyl group and conducting alkynylation treatment on silica gel and utilizing an efficient catalyst. Due to the fact that amylose and the derivative thereof have highly ordered spatial structures and inherent chiral environments, the chiral stationary phase shows excellent resolution capacity on various chiral racemates in a liquid chromatogram and can be applied to the field of various chiral chromatographic column packing materials.

The invention provides phenylene ethylenediamine derivatized beta-cyclodextrin bonded silica gel and an application thereof. According to the technical scheme, 6-(ethylenediamino)-beta-cyclodextrin and phthalic acid are taken as raw materials, thionyl chloride is taken as a chlorinating agent and a solvent, phenylene chloride is generated under a heating condition, then stirring is performed at the room temperature to enable 6-(ethylenediamino)-beta-cyclodextrin to be subjected to a reaction with phenylene chloride, 6(phenylene ethylenediamino)-beta-cyclodextrin is prepared, and a novel chiral stationary phase is prepared in a follow-up stage with 3-isocyanatopropylsiloxane as a coupling agent and ordered mesopore SBA-15 silica gel as a bonding matrix. The product has large specific surface area and large bonded quantity, the method is simple and lower in cost, and a chromatographic column filled with the stationary phase has longer service life because cyclodextrin is stable in performance and is connected with silica gel in a bonding manner. Besides, the product and a solute have various stereoselectivity functions, and by means of good permeability and small mass transfer resistance of SBA-15, the chiral separation speed is high and the effect is outstanding.

The invention discloses a benzyl nitrogen bridged calix [2] arene [2] triazine bonded silica stationary phase, its preparation method and its purpose. The preparation method comprises the following steps: a continuous solid-liquid reaction is used, 3-aminopropyltriethoxysilane silica and tetraazacalix[2]arene[2]triazineN2 is subjected to the reflux reaction under the temperature of 130 DEG C, tetraazacalix[2]arene[2]triazine bonded silica is prepared, then active hydrogen atoms are provided on the benzyl-substituted tetraazacalix[2]arene[2]triazine, and the benzyl nitrogen bridged calix[2]arene[2]triazine bonded silica stationary phase is prepared. The synthesized calixarene bonded stationary phase has the advantages of simple method, high bonding amount, stable bonding layer, low preparation cost, wide application field of preparation method and the like. possesses a traditional ODS reversed phase mode, and can provide a plurality of action sites of hydrogen bond action, Pi-Pi actionand the like, and provides separation possibility for substances which are difficult to separate.

Borono benzoylated beta-cyclodextrin bonded silica gel and uses thereof are provided. According to a technical scheme, a borono benzoylated beta-cyclodextrin ligand is firstly prepared by adopting ethylenediamine-beta-cyclodextrin, dicarboxyphenylboronic acid, N-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride as raw materials, and then a novel chiral stationary phase is prepared by adopting 3-isocyanato propylsiloxane as a coupling agent and adopting ordered mesopores SBA-15 silica gel as a bonding substrate. The bonded silica gel enriches chiral recognition sites through bonding a phenylboronic acid port substitute and a cyclodextrin cavity, and can generate chiral recognition functions with a neutral solute and an ionized solute through hydrogen bonds, dipoles, pi-pi, charge transfer, inclusion, and other synergistic effects, thus improving chiral separation capability and a chiral separation range. Test results prove that the bonded silica gel is large in specific area and high in bonding amount, and a chromatographic column is long in service lifetime because cyclodextrin is stable in performance and connected to the silica gel in a bonding manner.

The invention provides mesoporous silica (SiO2) and a preparation method and application thereof, and belongs to the field of silicone fillers. The specific surface area of the mesoporous SiO2 is 367-407 m2 / g, and the average pore diameter is 11.26-13.65 nm; and the content of surface hydroxyl is 0.498-0.541 mmol / g. The method for preparing the mesoporous SiO2 comprises the steps: a natural mineral (olivine or serpentine) is adopted as a silicon source, the mesoporous SiO2 is prepared through a one-step method, firstly the natural mineral silicon source is added into an acid, surfactant and porogenic agent mixed solution, the mixture is stirred for a period of time at the specific temperature to sufficiently react, after reacting is completed, centrifugal separation is conducted, a silicon-containing precursor is obtained, the silicon-containing precursor is subjected to still standing, filtered, washed and dried and then calcined at the high temperature, then the mesoporous SiO2 is obtained, and then the obtained mesoporous SiO2 is bonded to C18 and then used for high performance liquid chromatography fillers.

The invention discloses a preparation method of a tetraethylenepentamine carbon quantum dot / monomer co-bonded silica gel chromatographic packing. The preparation method comprises the following steps:reacting tetraethylenepentamine (TEPA) serving as a precursor substance and a medium with citric acid to prepare a mixture of functionalized carbon quantum dots (TEPACDs) and a TEPA monomer; and carrying out silanization treatment on the functionalized carbon quantum dots TEPACDs, and bonding the silanized functionalized carbon quantum dots TEPACDs to the surface of silica gel to prepare the silica gel chromatographic stationary phase Sil-TEPA / CDs formed by co-bonding of the TEPACDs and the TEPA monomer. Material characterization experiments show that the bonding amount of surface functional groups of the Sil-TEPA / CDs is large, and rich interaction sites can be provided for chromatographic separation of samples. Chromatographic separation experiments show that the hydrophilic chromatographic stationary phase Sil-TEPA / CDs prepared by the invention has good hydrophilic chromatographic selectivity, and has an excellent separation effect on basic nucleoside, amino acids and ginsenoside.

The invention relates to a mono-dispersed core-shell type polymer chromatography medium with a porous surface and a preparation method of the chromatography medium. The chromatography medium comprises an imporous polymer core and a porous polymer shell layer and can be used for controllably preparing a polymer shell layer hole structure. The preparation method comprises the following steps: firstly preparing a micro-scale mono-dispersed imporous polymer microsphere as a core; preparing a superfine emulsion from monomers, a cross-linking agent, an initiator and a pore-foaming agent, and enabling an organic phase to be uniformly attached to the surface of the imporous core; and finally separating an organic phase layer from the surface of the imporous core through solidification by virtue of thermal initiation polymerization reaction, so as to obtain the mono-dispersed core-shell type polymer chromatography medium with the porous surface.

The invention discloses a chitosan derivatization calix [4] arene bonded silica gel stationary phase. Firstly, chitosan reacts with 3-(4-brombutyl)-1-methylimidazole bromide to generate 3-(4-brombutyl)-1-methylimidazole bromide bonded chitosan; p-tert-butyl calix [4] arene is obtained through condensation polymerization of p-tert-butylphenol and formaldehyde, and 5,11,17,23-tetra-tert-butyl-25,27-di(3-bromopropoxy)-26,28-dyhydroxyl calix [4] arene is obtained through substitution reaction of p-tert-butyl calix [4] arene and 1,3-dibromopropane; the surface of silica gel is modified by means of glutaraldehyde, then condensation polymerization is conducted between silica gel and 3-(4-brombutyl)-1-methylimidazole bromide bonded chitosan, then reduction is conducted by means of sodium borohydride, and the product reacts with 5,11,17,23-tetra-tert-butyl-25,27-di(3-bromopropoxy)-26,28-dyhydroxyl calix [4] arene to generate the expected stationary phase. The stationary phase is easy to prepare, wide in application range, high in reproducibility and broad is application prospects.

The invention discloses a silica gel loaded polyamine and carboxyl bifunctional group composite adsorbing material. The bifunctional group composite adsorbing material comprises the following components in percentage by mass: 60.2-86.2% of silica gel, 6.1-24.0% of coupling agent, 1.7-14.5% of polyamine, and 1.7-14.5% of carboxyl, wherein the total mass of all the components is 100%. The invention further comprises a preparation method of the adsorbing material. The method comprises the step as follows: the porous silica gel is taken as a nonpolar rigid skeleton, activated, humidified, modified with the coupling agent, bonded with high molecular polyamine, and is grafted with carboxyl to obtain the bifunctional radical composite adsorbing material. The prepared adsorbing material has an excellent adsorptive property to heavy metal ions and can be used repeatedly after being regenerated; and the adsorbing material has a simple preparation technology, is easy to realize industrialization, is free from toxic solvent, is extensive in sources of raw material, can be applied to adsorption, separation and enrichment of heavy metal in water body, and realizes harmless treatment of waste water polluted by heavy metal.

A separation material based on silica gel substrate and preparation method thereof are provided. The separation material based on silica gel substrate is obtained by copolymerizing two or more silane reagents on the silica gel surface to form "non-polar / polar copolymerized stationary phase" and has the following formula, wherein NP denotes non-polar groups, which comprise C1-30 n-alkane groups, phenyl, and so on; P denotes polar groups, which comprise C1-12 n-alkane groups jointing with Cl, Br, CN, amino group, benzenesulfonic group, sulfonic group, carboxyl group, quaternary ammonium group, alcohol group, and so on. The preparation method of the above mentioned separation material comprises the following steps: acidification preprocessing of silica gel, hydrating, copolymerizing a mixture of non-polar silane reagent and polar silane reagent on the hydrated silica gel surface, filtering, washing, drying, and so on. The separation material of the present invention has both non-polar groups and polar groups, which can provide hydrophobic applied force and several kinds of polar applied forces, and thereby improve greatly the selectivity of separation and enrichment. The separation material obtained through the preparation method of the present invention has merits such as uniform and stable bonding groups on the surface, larger bonding amount and so on, and can be applied to liquid chromatogram chromatography and solid phase extraction.

The invention discloses a pyridine ionic liquid functionalized beta-cyclodextrin silica gel, which is prepared by the following steps of: ultrasonically dispersing silica gel microspheres and isocyanate silane into anhydrous pyridine, performing heating to 50-80DEG C under the protection of nitrogen or argon atmosphere, and carrying out stirring reaction for 24-48h; and adding beta-cyclodextrin into the reaction system, carrying out stirring reaction for 24-72h at a temperature of 60-80DEG C, adding halogenated isocyanate into the reaction system drop by drop, carrying out mechanical stirringreaction for 24-72h at a temperature of 60-100DEG C, carrying out centrifugal washing, and carrying out vacuum drying. Chromatographic separation selectivity results show that the pyridine ionic liquid functionalized beta-cyclodextrin silica gel prepared by the method has good chiral separation selectivity as compared with binaphthol chiral substances, benzoin chiral substances and alcohol chiralsubstances when used as a stationary phase.

The invention relates to an enrichment method of thiosulfated peptides, and applications thereof. According to the enrichment method, a polyaminodendritic polymer is taken as a carrier, covalent modification with iodoacetic acid-N-succinamide is carried out so as to prepare a water soluble high-molecular compound capable of realizing selective reaction with mercapto peptides; the water soluble high-molecular compound is mixed with a protein enzymatic hydrolysate fully, an obtained product is placed on an ultrafiltration membrane, selective blocking of the mercapto peptides on the ultrafiltration membrane is realized via high speed centrifugation; and at last, a disulfide bond reducing agent is added onto the ultrafiltration membrane to realize selective release of the thiosulfated peptides from the high-molecular compound at a certain temperature; and the thiosulfated peptides are obtained via high speed centrifugation. Operation of the enrichment method is simple; flux is high; selectivity is high; and the enrichment method is used for large-scale enrichment of the thiosulfated peptides in complex proteome samples.

When the high-contact-performance cylindrical gear serving as a driving gear and the high-contact-performance cylindrical gear serving as a driven gear are in meshing transmission, the relative curvature kr meets the condition that kr = k1-k2 = ar2 + br + c, r is the distance from a meshing point C to a node P, k1, k2, k3, k4, k5, k6, k7, k8, k7, k8, k8, k8, k8, k8, k8, k8, k8, k8, k8, k8, k8, k8, k8, k1, k2 are respectively curvatures of the tooth profile sigma 1 of the driving gear and the tooth profile sigma 2 of the driven gear at any meshing point C, and a, b and c are coefficients of a quadratic polynomial. Compared with the prior art, the cylindrical gear with the high contact performance can obtain higher contact, bending, abrasion and gluing bearing capacity and better lubricating performance.

The invention relates to a high molecular material, in particular to a preparation method for a three-dimensional ordered macroporous chelating resin used for absorbing precious metal ions in aqueous solution. The method comprises the following steps: (a) preparation of a silica colloidal crystal template; (b) preparation of a three-dimensional ordered macroporous crosslinked polystyrene porous material; (c) chloromethylation of the three-dimensional ordered macroporous crosslinked polystyrene porous material; (d) introduction of a hydrophilic cantilever; and (e) introduction of chelation groups. Compared with the traditional polystyrene resin microspheres, the prepared three-dimensional ordered macroporous chelating resin has the advantages that the resistance force of the regularly arranged pore canal systems is small, the chelation groups introduced on the hydrophilic cantilever can be changed according to the variety of the concrete absorbed metal ions, so that the purpose of highadsorption capacity to different metal ions is achieved.