The invention relates to a biological lysozyme compound preparation and a preparation method thereof. The preparation method comprises the following steps: dissolving lysozyme, amino acid, proteolytic enzyme, lysostaphin and peroxidase in water according to a certain proportion, transferring the mixed solution into a mixing tank, adding sorbitol, hydrated silica, aluminium hydroxide, glycerol, sodium lauroyl sarcosine, edible essence, cellulose gum, carrageenan, hydroxyethyl cellulose, sodium phytate, saccharin sodium, strontium chloride, trisodium phosphate, borneols, sodium borate, cinnabar, natrii sulfas exsiccatus and allantoin according to corresponding proportion, reacting for 47-49 h under a constant temperature condition of 38-42 DEG C to prepare the activated biological lysozyme compound preparation finished product. The biological lysozyme compound preparation belongs to a novel non-hormonic and non-antibiotic protein function preparation, has extremely great capacities of bacteriolyzing, reducing inflammation and enhancing immunity, and various effects of resisting various infections and promoting rehabilitation.