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Solid-phase micro extraction fiber extraction head and preparation method thereof

A technology for extracting fibers and extraction heads, which is applied in the field of preparation of solid-phase microextraction-coated fibers, can solve the problems of poor compatibility of biological samples, low selectivity, and few types of SPME coatings, and achieve small surface area and improved bonding volume effect

Inactive Publication Date: 2012-11-21
SOUTH CHINA NORMAL UNIVERSITY
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Problems solved by technology

[0006] The present invention aims at the aforementioned SPME coating types, low selectivity, and poor compatibility of biological samples, etc., combining the characteristics of high affinity, strong specificity, and good compatibility of biological samples with nucleic acid aptamers. SPME technology combines the advantages of less time-consuming, high efficiency, and simple operation. A layer of highly permeable porous polymer coating is prepared on the surface of silanized quartz fiber, and the nucleic acid aptamer is fixed to the coating by chemical bonding. In the porous structure, a novel SPME fiber extraction head based on nucleic acid aptamer / porous polymer coating was developed for high selection of trace and ultra-trace alkaloids, antibiotics or nucleotides in complex biological samples Sex Segregation and Enrichment

Method used

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  • Solid-phase micro extraction fiber extraction head and preparation method thereof
  • Solid-phase micro extraction fiber extraction head and preparation method thereof
  • Solid-phase micro extraction fiber extraction head and preparation method thereof

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Embodiment Construction

[0029] In this implementation, the preparation of an ATP nucleic acid aptamer / porous polymer coated SPME extraction head is taken as an example to describe the present invention in detail, but this does not limit the protection scope of the present invention.

[0030] Such as figure 1 As shown, the preparation steps of the novel SPME extraction head of ATP nucleic acid aptamer / porous polymer coating are as follows:

[0031] (1) Take a bare quartz fiber with a diameter of 125 μm and a length of 6.0 cm, corrode it with 10% hydrofluoric acid solution for 20 seconds, rinse it with distilled water three times after taking it out; soak the fiber in 1.0 mol / L NaOH alkaline solution at room temperature for 0.5 h, rinse three times with distilled water after taking it out; soak the quartz fiber in 1.0mol / L HCl pickling solution for 0.5h after alkali washing, rinse it three times with distilled water after taking it out; put the quartz fiber in a petri dish and place it in an oven for 1...

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Abstract

The invention discloses a preparation method for a solid-phase micro extraction fiber extraction head. The preparation method comprises the following steps of: firstly, carrying out silane treatment on a bare quartz fiber; secondly, adding an acrylamide function monomer, a cross-linking agent and an initiator in a polymerization solution and fully mixing to obtain a prepolymerization solution; thirdly, coating a porous polymer coating on the surface of the quartz fiber subjected to silane treatment; fourthly, carrying out ageing treatment on the quartz fiber; fifthly, repeating the second step to the fourth step for multiple times and repeatedly coating the porous polymer coating on the surface of the quartz fiber for multiple times; sixthly, carrying out bonding reaction on the porous polymer coating and the quartz fiber; and seventhly, adding the quartz fiber treated in the sixth step into an aptamer solution and carrying out bonding reaction at room temperature. According to the preparation method disclosed by the invention, the quartz fiber is treated by the steps, and thus the bonded amount of the aptamer on the surface of the fiber can be remarkably improved; and an SPME (Solid-Phase Micro Extraction) head with a novel aptamer / porous polymer coating can be prepared.

Description

technical field [0001] The invention belongs to the field of chemical analysis testing instruments, and relates to a preparation method of a solid-phase microextraction coating fiber, which is suitable for the separation and enrichment of trace alkaloids, antibiotics or nucleotide substances in complex biological samples. Background technique [0002] Efficient and highly selective sample pretreatment technology is an important step in the analysis of trace and ultra-trace substances in complex matrices such as biological, pharmaceutical, environmental samples and food. During chromatographic analysis, sample pretreatment is usually the most time-consuming and difficult-to-automate step, and is currently recognized as the most critical step. However, compared with the development of instrumental analysis technology, the progress of sample pretreatment technology has been relatively slow. Traditional sample pretreatment techniques such as liquid-liquid extraction, Soxhlet ex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26B01J20/285B01J20/30B01D15/08
Inventor 胡小刚叶婷婷蔡泉林范亚男柳晓飞伍莉莉
Owner SOUTH CHINA NORMAL UNIVERSITY
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