Preparation method of novel solid phase microextraction fiber based on nucleic acid aptamer/ nanogold/ porous polymer coating

Abstract

The invention discloses a preparation method of novel solid phase microextraction fiber based on a nucleic acid aptamer / nanogold / porous polymer coating. The method comprises the steps of washing quartz fiber with acetone, benzene, alkali and acid, and carrying out high-temperature activation and silanization treatment on the washed quartz fiber; feeding functional monomer, a cross-linking agent and an initiating agent into a polymerization solvent, then feeding the silanized quartz fiber, carrying out thermal initiation copolymerization reaction, and pulling out fiber after polymerization; carrying out silanization treatment on the prepared coating with a sulfydryl silanization reagent to obtain the quartz fiber with a sulphur modified porous polymer coating; putting the obtained quartz fiber into the prepared nanogold solution for bonding to obtain quartz fiber with a nanogold / porous polymer coating; putting the obtained quartz fiber into aptamer phosphate buffer solution, and bonding the quartz fiber with the nanogold / porous polymer coating and the aptamer for a certain period of time to obtain a solid phase microextraction head with the single-stranded DNA nucleic acid aptamer / nanogold / porous polymer coating.

Description

technical field [0001] The invention belongs to the field of chemical analysis and testing instruments, and relates to a preparation method of a novel solid-phase microextraction fiber based on nucleic acid aptamer / nano gold / porous polymer coating. It is suitable for high selectivity, rapid separation and enrichment of trace alkaloids, antibiotics or nucleotides in complex matrices such as biology, medicine, environmental samples and food. Background technique [0002] Sample pretreatment is the first step in sample analysis and a key step in the entire sample analysis process, which directly affects the accuracy and precision of the analysis. Its main purpose is to separate and enrich the components to be measured in the sample. However, traditional sample pretreatment techniques such as liquid-liquid extraction, solvent extraction, Soxhlet extraction, column chromatography, etc., generally have problems such as time-consuming, inefficient, large amount of toxic organic sol...

AI Technical Summary

Problems solved by technology

However, due to the limitations of molecular imprinting technology itself, there are still the following shortcomings: MIP synthesis needs to add a large number of template molecules, if the elution is not clean, template molecules will easily leak during extraction and interfere with the determination; MIP selectivity is mainly based on hydrogen bonding. , it is seriously interfered when extracted in polar solvents such as water, and biological samples often exist in the form of aqueous solution, so the application of MIP coatings in biological sample analysis is limited; MIP coatings are often extracted with benzene, toluene, chloroform or other organic Solvents pollute the environment; the rigid recognition "cavity" of the MIP coating is easily destroyed or deformed during the preparation process or extraction, and its specificity and affinity are not as good as those of biological recognition systems such as enzyme-substrate, antibody-receptor, etc. effect

The SPE method combined with the appropriate ligand-ligand recognition system can significantly improve the extraction selectivity and enhance the enrichment efficiency. However, compared with the SPME method, there are still problems such as more cumbersome operation, lower efficiency, time-consuming, and difficult to use with instruments. However, the application of aptamers to SPME methods has not been reported yet.

Images