48 results about "Mycosphaerella" patented technology

The invention discloses a bacillus belleus GT11 and application thereof. The bacillus belleus CT11 is collected at China General Microbiological Culture Collection Center on December 13th, 2016; the collection number is CGMCC No.13446; the bacillus belleus CT11 can be prepared into water agent, wettable powder or coating material and can be used for preventing and controlling the root rot diseases of pseudo-ginseng and plukenetia volubilis and Chinese cabbage clubroot. The invention provides an efficient, nontoxic, safe, non-residual and conveniently used biological agent for preventing and controlling the diseases such as root rot and clubroot; the effective active ingredient is bacillus belleus CT11 strain; the strain can generate the secondary metabolite antimicrobial active matter, is capable of degrading the cell wall of pathogenic bacteria and has an excellent growth-promoting effect for the crops, such as, plukenetia volubilis, pseudo-ginseng and Chinese cabbage; besides, the bacillus belleus CT11 has a better antibacterial effect for pseudo-ginseng phytophthora sojae, pseudo-ginseng fusarium solani, plukenetia volubilis gray mold, paddy bacterial mycosphaerella, and the like.

The invention belongs to the technical field of veterinary microbial additive preparation, and specifically relates to a strain of separated and screened Enterococcus faecium providing significant bacterial inhibition effects for common enteropathogenic bacteria such as staphylococcus aureus, escherichia coli and salmonella in breeding animals, and an application thereof. The probiotic Enterococcus faecium of the present invention is characterized in that: the strain is Enterococcus faecium HDRsEf1, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CCTCC NO:M2011031. The probiotic Enterococcus faecium of the present invention has characteristics of fast growth, high acid production capacity, strong stress resistance, safety, disease resistance and growth promotion, and can be used as the microbial feed additive for livestock and poultry feeds.

The invention relates to a salt-tolerant COD removal denitrifying microbial agent. The microbial agent consists of at least one of paracoccus sp FSTB-2, microbacterium kitamiense FSTB-4 and pseudomonas stutzeri FSTB-5, and further consists of at least one of paracoccus denitrificans DN-3 and methylobacterium phyllosphaerae SDN-3, wherein the paracoccus sp FSTB-2, the microbacterium kitamiense FSTB-4 and the pseudomonas stutzeri FSTB-5 are preserved in China General Microbiological Culture Collection Center on June 1, 2015, with preservation numbers of CGMCC No.10938, CGMCC No.10939 and CGMCC No.10940; and the paracoccus denitrificans DN-3 and the methylobacterium phyllosphaerae SDN-3 are disclosed in CN102465104A and CN102465103, and the preservation numbers of the paracoccus denitrificans DN-3 and the methylobacterium phyllosphaerae SDN-3 are CGMCC No.3658 and CGMCC No.3660. The microbial agent can be used for directly processing total nitrogen and COD in high-salinity wastewater, and the microbial agent can be also added to various biochemical reaction constructions so as to improve microbiological composition, optimize the salt tolerance of a microbial system in wastewater treatment and improve total nitrogen and COD removal rate of the entire process.

The invention relates to a molecular marker method for a melon blight resistance gene, belonging to the field of biotechnology. There are four AFLP molecular markers that are linked to the gene for resistance to blight blight, the marker EcoRI-TG / MseI-CTC200 is a 200bp band; the marker EcoRI-AT / MseI-CTG90 is a 90bp band; the EcoRI-TC / MseI-CAG60 is a 60bp band band; the marker EcoRI-TG / MseI-CTA70 is a 70bp band; the linkage distances are 2.0, 6.0, 5.4 and 6.0cM, respectively. Detect whether PI 420145 and its derivative varieties (lines) contain the gene through the molecular marker method provided by the present invention, predict its resistance level to vine blight, improve the selection efficiency, and help to accelerate the selection of excellent vine blight-resistant melon varieties in my country It is used in the production process to identify the purity of muskmelon varieties resistant to vine blight.

The invention discloses Plectosphaerella sp. EF01 separated from a root tuber of radix tetrastigme and application of the Plectosphaerella sp. EF01 in promotion of growth of the radix tetrastigme and increasing of total flavonoid content. An endophytic fungus, namely the Plectosphaerella sp. EF01, is separated from the root tuber of the radix tetrastigme, the Plectosphaerella sp. EF01 has effects of promoting growth of the radix tetrastigme and increasing the total flavonoid content, the fresh weight of the plant is increased by above two times, the length of a root is above two times greater than that of a control group, expression quantity of Th-exp in leaves, stems and roots of the radix tetrastigme is increased, and the total flavonoid content is increased by 87-121%.

The invention relates to the technical field of microorganisms and in particular relates to tetragenococcus halophilus. The tetragenococcus halophilus can be used for remarkably improving the contentof 4-vinylguaiacol in soy sauce or thick sauce, so that the aroma of the soy sauce is increased and the flavor and quality are improved. Therefore, the invention also relates to a method for preparingthe soy sauce or the thick sauce; the method comprises the step of adding the tetragenococcus halophilus provided by the invention before soy sauce mash is fermented or in a soy sauce mash fermentation process.

The invention discloses a soy sauce flavor Pixian bean paste production method based on 4-ethyl guaiacol fermentation intensification. The biological fungicide comprises yeast fungicide and tetragenococcus halophilus fungicide, wherein the yeast fungicide inoculates a 5Be' malt agar culture medium through zygosaccharomyces rouxii and intermediate aroma yeast to obtain fermentation broth; the tetragenococcus halophilus fungicide inoculates an improved mMRS culture medium to tetragenococcus halophilus to obtain the fermentation broth; then, porous starch, saccharose and glycerinum are added intothe fermentation broth to obtain corresponding fungicide. The yeast fungicide and the tetragenococcus halophilus fungicide are added into the Pixian bean paste in a normal after fermentation period to be subjected to joint fermentation, the content of 4-ethyl guaiacol can be improved by three times or more, the quality of a traditional Pixian bean paste product is comprehensively improved, and the traditional Pixian bean paste product is full of new life.

The invention relates to a Pediococcus pentosaceus R1 strain with in-vitro antioxidation activity and application thereof as an antioxidant in fermented meat products, belonging to the technical field of microbes. The Pediococcus pentosaceus R1 is collected at China General Microbiological Culture Collection Center (CGMCC), wherein the culture collection number is CGMCC No.9171, the collection date is May 16th, 2014, and the classification designation is Pediococcus pentosaceus. The Pediococcus pentosaceus R1 has favorable antioxidation activity in in-vitro antioxidation tests; the complete cell and intracellular extract have high capacity for removing hydroxy free radicals, and can inhibit the lipids from peroxidation; and the cell surface substances have antioxidation activity. Therefore, the Pediococcus pentosaceus R1 can be used as an antioxidant in fermented meat products to postpone the product deterioration caused by oxidation in the fermentation and storage process, thereby reducing the consumption of the antioxidant.

A carotenoid producing bacterium belonging to the genus Paracoccus that selectively produces canthaxanthin so that the amount thereof is not less than 90 percent by weight of the total amount of produced carotenoids including β-carotene, β-cryptoxanthin, echinenone, canthaxanthin, 3-hydroxyechinenone, 3′-hydroxyechinenone, zeaxanthin, phoenicoxanthin, adonixanthin, and astaxanthin. A method for producing canthaxanthin by culturing the above bacterium, and then collecting carotenoids from bacterial cells or a culture solution after the culturing.

The invention belongs to the technical field of veterinary microbial additive preparation, and specifically relates to a strain of separated and screened Enterococcus faecium providing significant bacterial inhibition effects for common enteropathogenic bacteria such as staphylococcus aureus, escherichia coli and salmonella in breeding animals, and an application thereof. The probiotic Enterococcus faecium of the present invention is characterized in that: the strain is Enterococcus faecium HDRsEf1, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CCTCC NO:M2011031. The probiotic Enterococcus faecium of the present invention has characteristics of fast growth, high acid production capacity, strong stress resistance, safety, disease resistance and growth promotion, and can be used as the microbial feed additive for livestock and poultry feeds.

The invention discloses a pediococcus pentosaceus strain, ferment produced thereby and the use of ferment in meat product, wherein the docket number of the R9(Pediiococcus Pentosaceus I9) strain in the Chinese Microbiological Culture Preservation Administration Commission Common Microbiological Center is CGMCC No.0918. The leaven prepared from the bacterial can be applied into the fermentation production of meat food.

The invention discloses a culture method of watermelon and melon. The method specifically includes the following steps of soaking plump watermelon and melon seeds in a saturated table salt water solution for 4-4.2 hours at the vacuum degree from -0.08 MPa to -0.085 MPa, scooping up the watermelon and melon seeds to be thoroughly flushed, taking garden soil where watermelon and melon is continuously planted for 2-5 years to serve as a seedling breeding substrate, sowing the watermelon and melon seeds in the seedling breeding substrate, thoroughly watering the seeds after sowing ends, inoculating young stems and leaves of watermelon and melon with melon type fusarium oxysporum through a needle point method three days after inoculation of mycosphaerella, and instantly and comprehensively spraying an allium caelureum extraction solution to the young stems and leaves of watermelon and melon 10-11 hours after inoculation. The cultured watermelon and melon is good in overall disease resistance, the later-period maintenance pesticide spraying frequency is greatly reduced, the damage of pesticides to fruits is reduced, and thus the healthiness degree is higher.

The invention relates to a method for screening mycosphaerella melonis-resistant IL 14 of cucumber-pickled cucumber introgression line. According to batch treatment of different concentrations, plants with good resistance are screened, and the method belongs to the field of biotechnology breeding. The method comprises the following steps: by taking wild species pickled cucumber as a female parent, cultivating cucumber 'Beijing jietou' as a male parent, and hybridizing to obtain hybrid F1; combining the hybrid F1 with a somaclone variation chromosome reduplication technology by virtue of embryo rescue, thereby obtaining a fertile inter-specific heterologous tetraploid new species which is named as Cucumis hytivus; and by taking the heterologous tetraploid new species Cucumis hytivus as a female parent, performing system backcrossing and self-cross selective breeding with the cultivated variety 'Beijing jietou', thereby obtaining 86 parts of cucumber inter-specific introgression line materials carried with wild species chromosome segments, and respectively numbering as IL1-IL86. The 86 parts of introgression line groups are screened by adopting the greenhouse seedling stage artificial inoculation identification technology, stable moderate resistance or more strains are obtained by virtue of inoculation treatment in spring and autumn, the inoculation treatment is performed by increasing the concentration of bacterium, and the mycosphaerella melonis resistance strain IL14 is screened.

The invention discloses a method for improving the quality of tobacco leaves by using blastococcus cavernae sp.. The strain is classified and named as Blastococcus cavernae sp., and is preserved in the China Center for Type Culture Collection CCTCC, and the preservation number is M2016739. Microorganism property analysis and 16S rDNA gene sequence analysis show that the strain is a novel bacterium. After an SYSUZL-2 agent is sprayed to tobacco leaves, and after alcoholization fermentation, the fragrance quality of the tobacco leaves is improved, the content of reduction sugar is increased, thecontents of proteins, cellulose, total nitrogen and nicotine are reduced, the ratios of sugar to nitrogen, sugar to nicotine and nitrogen to nicotine trend are increased, chemical components of the tobacco leaves are relatively well harmonized, and the sensory quality of the tobacco leaves is also remarkably improved.

The invention relates to a Pediococcus pentosaceus R1 strain with in-vitro antioxidation activity and application thereof as an antioxidant in fermented meat products, belonging to the technical field of microbes. The Pediococcus pentosaceus R1 is collected at China General Microbiological Culture Collection Center (CGMCC), wherein the culture collection number is CGMCC No.9171, the collection date is May 16th, 2014, and the classification designation is Pediococcus pentosaceus. The Pediococcus pentosaceus R1 has favorable antioxidation activity in in-vitro antioxidation tests; the complete cell and intracellular extract have high capacity for removing hydroxy free radicals, and can inhibit the lipids from peroxidation; and the cell surface substances have antioxidation activity. Therefore, the Pediococcus pentosaceus R1 can be used as an antioxidant in fermented meat products to postpone the product deterioration caused by oxidation in the fermentation and storage process, thereby reducing the consumption of the antioxidant.

Provided herein are compositions designed to reduce or prevent pneuomococcal infections, nasal carriage, nasal colonization, and central nervous system invasion. Provided herein is a composition comprising a polypeptide comprising the amino acid sequence of SEQ ID NO: 19 or a variant thereof that can elicit an anti-neuraminidase immune response. Further provided are methods of making and using the compositions disclosed herein. Specifically provided are methods of generating antibodies in a subject comprising administering to the subject an agent or composition taught herein. Also provided are methods of reducing or preventing nasal carriage or pneumococcal infection in a subject comprising administering to the subject a composition taught herein.