Plectosphaerella sp. EF01 separated from root tuber of radix tetrastigme and application of Plectosphaerella sp. EF01
A cloverleaf, non-integrity technology, applied in the field of strains for improving the growth and development of cloverleaf, can solve the problems of long time to form spherical tuberous roots and slow root development, and achieve the effects of increasing the content of total flavonoids and promoting growth.
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[0021] 1. Materials and methods
[0022] (1) Isolation and purification of endophytic fungi in root tubers
[0023] Wild clover collected in the mountainous area of Jinyun County, Zhejiang Province, take a spherical tuber with a diameter of about 2cm, wash off the surface soil with tap water, soak it in detergent for 10 minutes, rinse it with running water, and soak the tuber in a diluted 1 times saturated sodium hypochlorite (Antiformin) solution for 30 minutes, and finally rinsed with sterile water 3 times. Cut into small pieces with a length and width of 3-5 mm, place them in PDA medium containing 50 mg / L Str (streptomycin) (200 g / L of potatoes (peeled and cut into pieces, boiled in water and then filtered through gauze to remove slag), glucose 20 g / L and agar 14g / L, the solvent is deionized water, pH7.0), the cut surface faces the culture medium, 4-6 pieces per dish. At the same time, collect the sterile water used to rinse the tubers for the last time and spread them ...
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