197results about How to "Raise antibody levels" patented technology

The invention provides a porcine epidemic diarrhea virus inactivated vaccine. The porcine epidemic diarrhea virus inactivated vaccine contains the inactivated porcine epidemic diarrhea virus and an adjuvant, wherein the adjuvant is prepared from the following components in percentage by weight: 5% of squalane, 1% of oleic acid, 1% of Tween 80, 92% of 0.005M sodium citrate buffer solution, and 1% of beta-glucan, the porcine epidemic diarrhea virus is prepared by inactivating the porcine epidemic diarrhea virus strain PEDV-KB2013-4, is assigned with the microbial accession number of CGMCC No.12663, has the classification name of Porcine Epidemic Diarrhea Virus (PEDV), and is preserved in the China General Microbiological Culture Collection Center (CGMCC) on Aug 23, 2016, and the preservation address is the Institute of Microbiology of Chinese Academy of Sciences located in 3, Courtyard 1, West Beichen Road, Chaoyang District, Beijing.

The invention provides fermented feed special for piglets and a preparation method thereof. The fermented feed special for the piglets comprises the following components: 25-35 wt% of corn powder, 20-35 wt% of fermented soybean meal, 5-7 wt% of fish meal, 3-5 wt% of spray dried fish soluble protein powder, 2-3 wt% of spray dried blood meal, 2-5 wt% of compound enzyme preparation, 4-5 wt% of complex amino acid preparation, 8-12 wt% of low-protein whey powder, 5-10 wt% of lactose, 3-5% of sucrose, 1-2 wt% of glutamine, 0.05-0.1 wt% of xylo-oligosaccharide, 0.2-0.5 wt% of zinc oxide, 0.1-0.2 wt%of copper sulfate and 0.1-0.2 wt% of calcium carbonate. The fermented feed overcomes the disadvantage that the digestive enzyme in the piglets are not enough, balances the microbial community in the piglets, improves the resistance of the piglets, and has good diet-induced effect.

The invention discloses a magnetic separation and multicolor quantum dot marking-based method and a kit for quickly detecting anti-human chlamydia pneumoniae IgM and IgG antibodies simultaneously. The kit is composed of nanometer magnetic anti-human chlamydia pneumoniae antibody capturing beads, a multicolor quantum dot marked anti-human IgM antibody nanometer probe, a multicolor quantum dot marked anti-human IgG antibody nanometer probe, quality control products and a PBST buffer solution, wherein the quality control products comprise positive quality control products and negative quality control products; the positive quality control products are positive anti-human chlamydia pneumoniae IgM and IgG antibodies of a human chlamydia pneumoniae infected person and are positive serums, and the negative quality control products are negative anti-human chlamydia pneumoniae IgM and IgG and are negative human serums. The method has the advantages of simplicity, quickness and high sensitivity, and can detect the anti-human chlamydia pneumoniae IgM and IgG antibodies simultaneously.

The invention discloses a traditional Chinese herbal microecological preparation for preventing and treating piglet bacterial dysentery and a preparation method thereof, and belongs to the technical field of veterinary traditional Chinese medicine. The traditional Chinese herbal microecological preparation is mainly composed of the following traditional Chinese herbals in parts by weight: 1 to 10 parts of Chinese pulsatilla root, 3 to 20 parts of coptis, 5 to 15 parts of phellodendron, and 6 to 25 parts of ash bark; and also comprises the following bacterium liquids in parts by volume: 1 to 3 parts of lactobacillus acidophilus liquid, 1 to 6 parts of enterococcus faecalis liquid, and 1 to 6 parts of bacillus subtilis liquid; wherein the ratio of the total weight of the bacterium liquid to the total weight of the traditional Chinese herbals is (1-5):(40-50). The microecological preparation is prepared by compounding traditional Chinese herbals and beneficial bacteria and has the characteristics of no drug resistance, little side or toxic effect, and difficulty in generating harmful residues in the prevention and treatment of piglet bacterial dysentery. The beneficial bacteria can generate antibacterial substances and compete with harmful bacteria to occupy the ecological niche, thus the propagation of pathogenic bacteria is inhibited, the flora balance in the intestinal tract is modulated and balanced, the piglet diarrhea rate is reduced, and the piglet growth is promoted.

The invention provides a preparation method of a bivalent vaccine by taking newcastle disease virus La Sota strain and infectious bronchitis virus N-S multi-epitope protein as antigen. To immunize 21-day-old chickens, the bivalent vaccine prepared by the method disclosed by the invention can be used for simultaneously preventing newcastle disease and nephrotropic infectious bronchitis virus infection, and the antibody level of the bivalent vaccine is higher than that of respectively immunizing by single vaccine, so that immunological stress is effectively relieved and immunity time is reduced.

The invention discloses a traditional Chinese medicine micro-ecological preparation, and belongs to the technical field of veterinary traditional Chinese medicines and feed additives. The traditionalChinese medicine micro-ecological preparation is mainly prepared from the following raw materials in parts by weight: 2-10 parts of codonopsis pilosula, 2-10 parts of milkvetch root, 2-8 parts of poria cocos, 1-5 parts of medicated leaven, 1-6 parts of malt, 1-5 parts of hawthorn fruit, 1-3 parts of licorice root and 1-3 parts of betel nut; a bacterium solution comprises the following components in parts by volume: 1-3 parts of a lactobacillus acidophilus bacterium solution, 1-6 parts of an enterococcus faecalis bacterium solution and 1-8 parts of a bacillus subtilis bacterium solution, and the ratio of the total weight of the bacterium solution to the total weight of the raw materials is (1-5):(45-55). According to the traditional Chinese medicine micro-ecological preparation provided bythe invention, the eight pure natural Chinese herbal medicines are compounded with three probiotics to prepare the micro-ecological preparation which can generate various hydrolytic enzymes in animals, promote the decomposition of protein, starch, fat and hemicellulose, and facilitate digestion and absorption of nutrients in feeds.

The invention belongs to the technical field of biology, and particularly relates to a tetravalent influenza virus subunit vaccine and a preparation method thereof. Each dose of the tetravalent influenza virus subunit vaccine contains H1N1, H3N2 and two types of B; and the tetravalent influenza virus subunit vaccine is prepared by virus inoculation, virus multiplication culture, allantoic fluid harvesting, clarification, inactivation, ultrafiltration concentration, cracking and ultracentrifugal purification, mixing, filtration sterilization, split charging and packaging, wherein the inactivation process comprises the following steps: firstly, adding a carboxymethyl glucan solution into monovalent virus harvesting liquid, and then adding formaldehyde for inactivation. According to the method disclosed by the invention, the content of free formaldehyde in the prepared tetravalent influenza virus subunit vaccine is reduced, the storage life of the vaccine is prolonged, and the antibody level after vaccine immunization is increased.

The invention relates to a veterinary Chinese medicinal composition for improving growth performance and enhancing the non-specific immune function of an organism. The composition is prepared from the following raw material medicaments: Chinese pulsatilla root, astragalus, large head atractylodes rhizome, indigowoad root, baical skullcap root, bupleurum and rhubarb. The invention also relates to a veterinary medicament prepared from the veterinary Chinese medicinal composition, a method for preparing the veterinary medicament and the application of the veterinary Chinese medicinal composition. The veterinary Chinese medicinal composition has the effects of strengthening body resistance, eliminating evil, clearing away heat and toxic material and enhancing the non-specific immune function of the organism and can treat various atypical symptoms such as low immune function of the organism, immune tolerance, endogenous toxin, stress and the like, the decrease of the growth performance and damp-heat symptoms such as anemopyretic cold, flu, gumboro disease, enterotoxin syndrome and the like.

The invention relates to a preparation method and application of high-purity polysaccharopeptide. The preparation method comprises extracting and purifying polysaccharopeptide coarse powder and obtaining the polysaccharopeptide with high polysaccharide content and high protein level. The polysaccharopeptide can improve antibody levels of chickens, accordingly improve immunity of the chickens, be used for preparing human medicines or beast medicines with immunity or/and physiological accommodation activity and particularly serve as vaccine immunopotentiator of beasts and birds.

The invention provides a Muscovy duck parvovirus inactivation vaccine which comprises an antigen and a vaccine adjuvant, wherein the antigen is inactivated Muscovy duck parvovirus, and the collection number of the Muscovy duck parvovirus is CGMCC No.8504. The Muscovy duck parvovirus inactivation vaccine provided by the invention is prepared by the steps of screening a Muscovy duck parvovirus YBMDP strain with low toxicity and high immunogenicity; inoculating and collecting the virus liquid; performing ultrafiltration concentration and formaldehyde solution inactivation; adding an oil adjuvant and mixing and emulsifying to obtain the vaccine. The vaccine provided by the invention can improve the antibody level of Muscovy duck, guarantee the maternal antibody level of the filial generation and prevent the duckling parvovirus infection caused by the Muscovy duck parvovirus; the vaccine has the advantages of high efficiency and good safety.

The invention provides a traditional Chinese medicinal composition for veterinary use. The traditional Chinese medicinal composition contains a Radix Astragali and Chinese angelica extract product which is prepared from 10-90 parts by weight of Radix Astragali and 10-90 parts by weight of Chinese angelica. The traditional Chinese medicinal composition for veterinary use can be used for preventing and curing livestock viral diseases and has characteristics of high efficiency and low toxicity. The traditional Chinese medicinal composition for veterinary use can be combined with a vaccine for livestock, so as to raise the immune effect of the vaccine. Through the combined application method, treatment effect also can be enhanced. The traditional Chinese medicinal composition for veterinary use has advantages of simple preparation method and low cost. The prepared preparation product requires small dose and is convenient to store, carry and use.