197results about How to "Raise antibody levels" patented technology

The invention discloses a method for raising traditional Chinese medicine chicken. The method comprises the following steps of: 1) selecting a traditional Chinese medicine formula; 2) fermenting components according to the traditional Chinese medicine formula to obtain fermentation liquid and fermentation bodies; and 3) applying the fermentation liquid and the fermentation bodies obtained in the step 2) to different feeding stages of the traditional Chinese medicine chicken. According to the invention, hormones and antibiotics are not needed during the raising process of the chicken, and the effects of the traditional Chinese medicine components are maximally brought into play after the components are fermented. The method has the characteristics of being natural, avoiding toxic and side effects and drug resistance and the like, can effectively reduce drug residues in the chicken products, obviously improve the constitution of the chicken, improve the immunity and the antibody level of organisms and reduce diseases, has functions of health care and curing diseases, and can improve the ecological environment.

The invention discloses medicinal steamed bread for treating diabetes. Every 100 grams of steamed bread contain 3-10 percent by weight of medicament which is prepared from the following raw materials in parts by weight: 10-6 parts of Chinese yam, 10-5 parts of gorgon euryale seed, 10-3 parts of fragrant solomonseal rhizome, 8-3 parts of common anemarrhena, 8-3 parts of Mongolian snakegourd root, 8-3 parts of sophora flower, 8-2 parts of sealwort, 8-2 parts of corn stigma, 6-2 parts of dwarf lilyturf root, 6-2 parts of asparagus, 6-2 parts of propolis, 6-1 parts of curcumin and 6-1 parts of mulberry leaf. The medicinal steamed bread can be used for relieving, improving, preventing and treating diabetes type I and diabetes type II, so that the aims of relieving, improving, preventing and treating with medicinal foods are achieved.

The invention provides a porcine epidemic diarrhea virus inactivated vaccine. The porcine epidemic diarrhea virus inactivated vaccine contains the inactivated porcine epidemic diarrhea virus and an adjuvant, wherein the adjuvant is prepared from the following components in percentage by weight: 5% of squalane, 1% of oleic acid, 1% of Tween 80, 92% of 0.005M sodium citrate buffer solution, and 1% of beta-glucan, the porcine epidemic diarrhea virus is prepared by inactivating the porcine epidemic diarrhea virus strain PEDV-KB2013-4, is assigned with the microbial accession number of CGMCC No.12663, has the classification name of Porcine Epidemic Diarrhea Virus (PEDV), and is preserved in the China General Microbiological Culture Collection Center (CGMCC) on Aug 23, 2016, and the preservation address is the Institute of Microbiology of Chinese Academy of Sciences located in 3, Courtyard 1, West Beichen Road, Chaoyang District, Beijing.

The invention discloses methods for preparing a duck virus hepatitis strain and an inactivated vaccine. The strain is obtained with the method comprising the following steps of: collecting duck hepatitis material with obvious pathological changes in a duck farm where duck virus hepatitis prevails, washing with bi-anti-sterilization PBS, diluting and grinding, freeze thawing twice and performing centrifugal separation; diluting virus separated by sterilization PBS, vaccinating chick embryo, selecting chick embryo with classic pathological changes that is dead 48-96h after vaccination, respectively gaining chick embryo liquid, transferring for 18-25 generations continuously to obtain a duck virus hepatitis virus chick embryo suitable strain. The strain can be in production, vaccinated in the chick embryo to gain chick embryo liquid, and is emulsified after vaccination to prepare the inactivated vaccine. The inactivated vaccine is high in safety, has good immune efficacy to young ducks, long immune time for breeding ducks and high level of antibody of breeding ducks, and the young ducks bred by the breeding ducks can resist duck virus hepatitis strains.

The invention discloses eco-nutritional compound feed for laying hens at an egg-laying peak and a preparation method of the eco-nutritional compound feed. The eco-nutritional compound feed for the laying hens at the egg-laying peak comprises components in percentage by weight as follows: corn, bean pulp, rice bran, calcium hydrophosphate, rock flour, baking soda, sodium chloride, soybean oil, compound trace elements for the laying hens, compound vitamins for the laying hens, 50% choline chloride, a bacillus coagulans preparation, a fructo-oligosaccharide preparation, an DL-methionine, compound enzyme for the laying hens, phytase, montmorillonite and zeolite powder. The nutritional requirement of the laying hens can be met, the immunity and the anti-stress capacity of the laying hens can be effectively improved, the disease rate is reduced, the egg laying rate is increased, the lasting time of the peak is prolonged, the quality of eggs is improved, the environment of henhouses can be improved, and the eco-nutritional compound feed is a novel laying egg feed product integrating nutrition, the anti-disease property, promotion of a high yield and pollution-free property.

The invention provides fermented feed special for piglets and a preparation method thereof. The fermented feed special for the piglets comprises the following components: 25-35 wt% of corn powder, 20-35 wt% of fermented soybean meal, 5-7 wt% of fish meal, 3-5 wt% of spray dried fish soluble protein powder, 2-3 wt% of spray dried blood meal, 2-5 wt% of compound enzyme preparation, 4-5 wt% of complex amino acid preparation, 8-12 wt% of low-protein whey powder, 5-10 wt% of lactose, 3-5% of sucrose, 1-2 wt% of glutamine, 0.05-0.1 wt% of xylo-oligosaccharide, 0.2-0.5 wt% of zinc oxide, 0.1-0.2 wt%of copper sulfate and 0.1-0.2 wt% of calcium carbonate. The fermented feed overcomes the disadvantage that the digestive enzyme in the piglets are not enough, balances the microbial community in the piglets, improves the resistance of the piglets, and has good diet-induced effect.

The invention discloses an oil emulsion vaccine for broilers and a preparation method thereof. The oil emulsion vaccine comprises a water phase and an oil phase, wherein the water phase contains concentrated and inactivated ND (newcastle disease) and H9 AI (avian influenza) venom and Tween-80; the oil phase contains an oil adjuvant formed by white oil, Span-80 and aluminium stearate; the volume ratio of the water phase to the oil phase is (1:1.6) to (1:2); and the kinematic viscosity (40 DEG C) of the selected white oil is 4-8mm<2> / s. The oil emulsion vaccine has the characteristics of easiness in absorption, quick effect and high level of the generated antibodies, is suitable for growth of the broilers, can effectively increase the immune levels of the broilers, can effectively protect the broilers from being invaded by ND and H9 AI viruses and avoids ND and H9 AI in the immune phase.

The invention discloses a traditional Chinese medicine microecological preparation for meat chicken, a preparation method and applications thereof, and belongs to the technical fields of veterinary traditional Chinese medicine and feed additive. The traditional Chinese medicine microecological preparation is mainly composed of the following traditional Chinese herbals in parts by weight: 2 to 10 parts of radix codonopsis, 2 to 10 parts of radix astragali, 2 to 8 parts of poria cocos, 1 to 5 parts of medicated leaven, 1 to 6 parts of malt, 1 to 5 parts of haw, 1 to 3 parts of licorice, and 1 to 3 parts of betel nut; and also comprises the following components in parts by volume: 1 to 3 parts of lactobacillus acidophilus liquid, 1 to 6 parts of enterococcus faecalis liquid, and 1 to 8 parts of bacillus subtilis liquid; wherein the total weight ratio of the traditional Chinese herbals to bacterium liquids is (1-5):(45-55). Through compounding eight pure natural traditional Chinese herbals mentioned above and three beneficial bacteria, the preparation can generate a plurality of hydrolytic enzymes in animals, and the enzymes can promote the degradation of protein, starch, fat, and hemicelluloses, so the preparation can promote the digestion and absorption of nutrients in feed.

The invention discloses a magnetic separation and multicolor quantum dot marking-based method and a kit for quickly detecting anti-human chlamydia pneumoniae IgM and IgG antibodies simultaneously. The kit is composed of nanometer magnetic anti-human chlamydia pneumoniae antibody capturing beads, a multicolor quantum dot marked anti-human IgM antibody nanometer probe, a multicolor quantum dot marked anti-human IgG antibody nanometer probe, quality control products and a PBST buffer solution, wherein the quality control products comprise positive quality control products and negative quality control products; the positive quality control products are positive anti-human chlamydia pneumoniae IgM and IgG antibodies of a human chlamydia pneumoniae infected person and are positive serums, and the negative quality control products are negative anti-human chlamydia pneumoniae IgM and IgG and are negative human serums. The method has the advantages of simplicity, quickness and high sensitivity, and can detect the anti-human chlamydia pneumoniae IgM and IgG antibodies simultaneously.

The invention relates to piglet feed and especially relates to fermented type mixed feed for piglets, which is prepared reasonably after feed raw materials subjected to fermentation treatment are added. The fermented type mixed feed for piglets comprises the following components in parts by weight: 584.19-651.79 parts of corn, 178-200 parts of bean meal, 19-31 parts of wheat bran, 50-90 parts of fermented raw materials, 25-30 parts of sucrose, 20-40 parts of fishmeal, 5-8 parts of stone powder, 8-12 parts of soybean oil, 8-12 parts of calcium hydrogen phosphate, 3.0-3.5 parts of sodium chloride, 1.0-1.5 parts of choline chloride, 0.6-0.8 part of methionine, 3.5-4.0 parts of lysine hydrochloride, 1.0-1.3 parts of threonine, 0.26-0.34 part of tryptophan, 0.15-0.17 part of phytase, 0.9-1.1 parts of trace element premixed feed, 1.0-1.1 parts of vitamin premixed feed and 0.4 part of probiotics for pigs. The fermented type mixed feed for piglets has long-term economic benefits for feed production enterprises and piglet raisers and also can generate huge environmental protection benefits for the society.

The invention discloses a traditional Chinese herbal microecological preparation for preventing and treating piglet bacterial dysentery and a preparation method thereof, and belongs to the technical field of veterinary traditional Chinese medicine. The traditional Chinese herbal microecological preparation is mainly composed of the following traditional Chinese herbals in parts by weight: 1 to 10 parts of Chinese pulsatilla root, 3 to 20 parts of coptis, 5 to 15 parts of phellodendron, and 6 to 25 parts of ash bark; and also comprises the following bacterium liquids in parts by volume: 1 to 3 parts of lactobacillus acidophilus liquid, 1 to 6 parts of enterococcus faecalis liquid, and 1 to 6 parts of bacillus subtilis liquid; wherein the ratio of the total weight of the bacterium liquid to the total weight of the traditional Chinese herbals is (1-5):(40-50). The microecological preparation is prepared by compounding traditional Chinese herbals and beneficial bacteria and has the characteristics of no drug resistance, little side or toxic effect, and difficulty in generating harmful residues in the prevention and treatment of piglet bacterial dysentery. The beneficial bacteria can generate antibacterial substances and compete with harmful bacteria to occupy the ecological niche, thus the propagation of pathogenic bacteria is inhibited, the flora balance in the intestinal tract is modulated and balanced, the piglet diarrhea rate is reduced, and the piglet growth is promoted.

The invention provides a preparation method of a bivalent vaccine by taking newcastle disease virus La Sota strain and infectious bronchitis virus N-S multi-epitope protein as antigen. To immunize 21-day-old chickens, the bivalent vaccine prepared by the method disclosed by the invention can be used for simultaneously preventing newcastle disease and nephrotropic infectious bronchitis virus infection, and the antibody level of the bivalent vaccine is higher than that of respectively immunizing by single vaccine, so that immunological stress is effectively relieved and immunity time is reduced.

The invention discloses a porcine epidemic diarrhea virus genetic engineering vaccine and a preparation method thereof, which belongs to the field of veterinary biological products. Core proteins of hepatitis B virus are used as a vector, a porcine epidemic diarrhea virus protective antigen is inserted between a 79 amino acid to 80 amino acid of the vector by using a molecular biology method to form the genetic engineering vaccine for preventing the porcine epidemic diarrhea virus. The preparation method of the vaccine is simple, a great amount of antigen proteins can be prepared, theconsumed time is short, the expression amount is high, the production cost is greatly reduced, and the mass production is facilitated. The porcine epidemic diarrhea sub-unit vaccine comprising HBcAg-PEDV-COE recombinant proteins prepared in the invention is good in immunity effect, small in immunity dose, and capable of effectively preventing the infection of the porcine epidemic diarrhea virus.

The invention relates to preparation and application of an efficient cell inactivation vaccine against new duck reovirus disease. The preparation method comprises the following steps: an antigen preparation step: inoculating a virus NDRV-TH11 strain with immortalized duck embryo fibroblast to obtain cytotoxicity with 80% of cytopathic effect, and performing cryopreservation at -20 DEG C; and a vaccine preparation step: inactivating the obtained cytotoxicity and mixing with a homemade nano adjuvant and stirring uniformly to obtain the efficient cell inactivation vaccine against new duck reovirus disease. After the duck is immunized by the efficient cell inactivation vaccine against new duck reovirus disease prepared in the invention, the duck can effectively resist the attack of a virulent strain of the new duck reovirus.

The invention discloses a traditional Chinese medicine micro-ecological preparation, and belongs to the technical field of veterinary traditional Chinese medicines and feed additives. The traditionalChinese medicine micro-ecological preparation is mainly prepared from the following raw materials in parts by weight: 2-10 parts of codonopsis pilosula, 2-10 parts of milkvetch root, 2-8 parts of poria cocos, 1-5 parts of medicated leaven, 1-6 parts of malt, 1-5 parts of hawthorn fruit, 1-3 parts of licorice root and 1-3 parts of betel nut; a bacterium solution comprises the following components in parts by volume: 1-3 parts of a lactobacillus acidophilus bacterium solution, 1-6 parts of an enterococcus faecalis bacterium solution and 1-8 parts of a bacillus subtilis bacterium solution, and the ratio of the total weight of the bacterium solution to the total weight of the raw materials is (1-5):(45-55). According to the traditional Chinese medicine micro-ecological preparation provided bythe invention, the eight pure natural Chinese herbal medicines are compounded with three probiotics to prepare the micro-ecological preparation which can generate various hydrolytic enzymes in animals, promote the decomposition of protein, starch, fat and hemicellulose, and facilitate digestion and absorption of nutrients in feeds.

The invention relates to an inactivated lactic acid bacteria vaccine adjuvant, which belongs to the field of a biological medicine. A main component of the adjuvant is inactivated lactic acid bacteria, and the adjuvant is prepared by inactivating the live lactic acid bacteria through a conventional method. The inactivated lactic acid bacteria can be taken as various vaccine adjuvant for human or animals, used for enhancing vaccine immunization effect, and has a wide application prospect.

The invention discloses a combined powder of traditional Chinese medicine and western medicine for enhancing chicken immunity and a preparation method thereof, which is characterized in that: the combined powder is prepared by evenly mixing the raw materials according the following mix ratio with parts by weight: 3 to 6 parts of levamisole hydrochloride, 6 to 10 parts of astragalus polysaccharide, and the glucose anhydrous is added till 100g, thus preparing the powder. The combined powder has the advantages of fully activating the immune response ability, promoting the secretion of immunocytes and improving the level of antibodies generated by the body. The combination of the traditional Chinese medicine and the western medicine has complementary effect, which has no residues in the poultry body, no drug resistance and can effectively enhance the immunity of the chickens.

The invention discloses a method for ecologically rearing chickens in undergrowth, and belongs to the technical field of livestock rearing. According to the method for ecologically rearing the chickens in the undergrowth, a chicken house is kept dry and clean by changing the chicken house environment, due to the chicken rearing mode with the combination of cage rearing and free rearing at the appropriate temperature, the health of the chickens is ensured, and the special flavor is not influenced by the cage-rearing mode. The method is beneficial to harmonious coexistence of the chickens, grass, forests and lands, so that the chickens live a better life, the ecological environment is protected, and the incomes of farmers are increased.

The invention belongs to the technical field of biology, and particularly relates to a tetravalent influenza virus subunit vaccine and a preparation method thereof. Each dose of the tetravalent influenza virus subunit vaccine contains H1N1, H3N2 and two types of B; and the tetravalent influenza virus subunit vaccine is prepared by virus inoculation, virus multiplication culture, allantoic fluid harvesting, clarification, inactivation, ultrafiltration concentration, cracking and ultracentrifugal purification, mixing, filtration sterilization, split charging and packaging, wherein the inactivation process comprises the following steps: firstly, adding a carboxymethyl glucan solution into monovalent virus harvesting liquid, and then adding formaldehyde for inactivation. According to the method disclosed by the invention, the content of free formaldehyde in the prepared tetravalent influenza virus subunit vaccine is reduced, the storage life of the vaccine is prolonged, and the antibody level after vaccine immunization is increased.

The present invention belongs to the technical field of murrain vaccine preparation, and in particular relates to a Streptococcus suis type 2 trimaceral subunit vaccine and a preparation method thereof. The key technology is that recombinant Escherichia coli such as Escherichia coli BL21 / pET-28a-1036N, Escherichia coli BL21 / pET-28a-0197 and Escherichia coli BL21 / pET-28a-enolase is prepared, which can excrete and express Streptococcus suis type 2 antigen albumen 1036N,0197 and enolase, and which is preserved as CCTCC NO M208147, CCTCC NO M208146 and CCTCC NO M208148 in China Center for Type Culture Collection respectively. The present invention also discloses a preparation method and a use suitable for the trimaceral subunit vaccine of Streptococcus suis type 2.

The invention relates to a veterinary Chinese medicinal composition for improving growth performance and enhancing the non-specific immune function of an organism. The composition is prepared from the following raw material medicaments: Chinese pulsatilla root, astragalus, large head atractylodes rhizome, indigowoad root, baical skullcap root, bupleurum and rhubarb. The invention also relates to a veterinary medicament prepared from the veterinary Chinese medicinal composition, a method for preparing the veterinary medicament and the application of the veterinary Chinese medicinal composition. The veterinary Chinese medicinal composition has the effects of strengthening body resistance, eliminating evil, clearing away heat and toxic material and enhancing the non-specific immune function of the organism and can treat various atypical symptoms such as low immune function of the organism, immune tolerance, endogenous toxin, stress and the like, the decrease of the growth performance and damp-heat symptoms such as anemopyretic cold, flu, gumboro disease, enterotoxin syndrome and the like.

The invention discloses an astragalus mongholicus formulation for veterinary use and a preparation method thereof, and belongs to the technical of extraction and application of effective components of plant traditional Chinese medicine. The medicine comprises two active ingredients including astragalus polysaccharide and astragalus polypeptide, and the form of the medicine is injection, oral liquid or powder. The preparation method includes comprehensively utilizing an enzyme hydrolysis extraction technology to effectively extract the high-activity astragalus polypeptide and the high-content high-purity astragalus polysaccharide from raw materials of astragalus mongholicus and effectively preparing the astragalus mongholicus formulation. The technical problems of comprehensive extraction and preparation are solved, further systematic and deep research on the astragalus polysaccharide and the astragalus polypeptide is facilitated, yield and purity are greatly increased, resource waste is reduced, cost is lowered, and industrialized production is facilitated.

The invention relates to a preparation method and application of high-purity polysaccharopeptide. The preparation method comprises extracting and purifying polysaccharopeptide coarse powder and obtaining the polysaccharopeptide with high polysaccharide content and high protein level. The polysaccharopeptide can improve antibody levels of chickens, accordingly improve immunity of the chickens, be used for preparing human medicines or beast medicines with immunity or / and physiological accommodation activity and particularly serve as vaccine immunopotentiator of beasts and birds.

The invention provides a Muscovy duck parvovirus inactivation vaccine which comprises an antigen and a vaccine adjuvant, wherein the antigen is inactivated Muscovy duck parvovirus, and the collection number of the Muscovy duck parvovirus is CGMCC No.8504. The Muscovy duck parvovirus inactivation vaccine provided by the invention is prepared by the steps of screening a Muscovy duck parvovirus YBMDP strain with low toxicity and high immunogenicity; inoculating and collecting the virus liquid; performing ultrafiltration concentration and formaldehyde solution inactivation; adding an oil adjuvant and mixing and emulsifying to obtain the vaccine. The vaccine provided by the invention can improve the antibody level of Muscovy duck, guarantee the maternal antibody level of the filial generation and prevent the duckling parvovirus infection caused by the Muscovy duck parvovirus; the vaccine has the advantages of high efficiency and good safety.

The invention relates to a composition and proportion method of a feed for enabling hens to lay eggs with protein which is higher than that of common eggs. Commonly, common raw materials of a laying hen feed used in China are formed by microelements, amino acids substances, soybeans including soya bean meals, cottonseed meals, rapeseed dregs, distiller grains and the like, corns, mountain flour including triple superphosphate, calcium carbonate and the like, and a necessary coloring agent. The formula used by the invention is increased and decreased on the basis of a formula of a conventional feed, and is particularly updated on the aspect of improving the protein content; miscellaneous meals are increased while alfalfa meal and cup plant powder are increased; biological microbial inoculums including bacillus licheniformis, bacillus subtilis and the like for accelerating proteins to absorb are increased; the coloring agent does not use chemical additives; the chemical additives are replaced by chili powder, marigold powder, carrot powder, purple sweet potato powder and the like for people. The protein content of the laid eggs is obviously higher than that of the common eggs and is commonly more than 13%; the contents of lecithin, lututrin, various types of minerals and vitamins are higher.

The invention belongs to the technical field of microbes, and particularly relates to a porcine epizootic diarrhea virus variant strain (PEDV-HNCADC-2017, preservation number: CCTCC NO:V201766) and application thereof. The variant strain provided by the invention realizes the generation passage to 156th generation in VERO cells of 10-percent blood serum medium; the TCID50 of the VERO cells can reach 107.7 / 0.1mL; the toxic valence is high; the reaction can be performed with porcine positive blood serum and Balb / c mouse positive blood serum; the immunofluorescence reaction is stronger. The prepared weak toxicity live vaccine can be used for preventing the porcine epizootic diarrhea diseases; the antibody generation is fast; the high antibody level can be generated after the immunization for2 weeks. The prepared inactivated vaccine has high safety; toxin expelling is avoided after the immunization; the strain can be used for preventing and controlling the porcine epizootic diarrhea diseases of sows and one-day-old piglets. When the combined immunization is used, the immunization effect can be obviously improved.

G2 protein is obtained through prokaryotic recombinant expression, the protein fragment has good immunogenicity, can stimulate an organism to generate a high antibody level, and has better immunogenicity and immune protection performance compared with the whole G protein and other fragments; a carbon nano tube carrier sub-unit vaccine prepared on the basis of combination of a G2 protein and a functional carbon nano tube attacks an MSRV FJ985 strain 21 days after immunizing weever, the immune protection rate of the weever is 86% and is higher than the protection rate reported in the prior art, on the basis of selection of specific protein fragments, and compared with the original G protein and other G protein fragments obtained based on immunoassay, the G protein fragment has better protective efficacy, can generate a better protective effect after immunizing weever, and can enable the weever to effectively resist attack of MSRV virulent virus.

The invention discloses a crop straw silage leavening agent, prepared from the following raw materials in percentage by weight: 50-60% of a microbial strain, 15-25% of a protective agent, 1-5% of a compound additive, 5-15% of carrier defatted rice bran, and 5-15% of light calcium carbonate, wherein the microbial strain is obtained by mixing of following bacterial powder in percentage by weight: 20-25% of lactobacillus plantarum, 20-25% of pediococcus acidilactici, 10-20% of lactobacillus acidophilus, 10-15% of enterococcus faecalis, 10-15% of lactobacillus buchneri, and 10-15% of lactobacillus paracasei. The leavening agent disclosed by the invention can be applied to preparation of a crop straw silage, and the invention also discloses a preparation method of the crop straw silage. The leavening agent disclosed by the invention can be used for fermenting various crop straws into available feed products, antibiotics are not used at all, and the digestivity to feeds of animals is improved, so that the crop straws are changed from waste into valuable, and the cost of raising livestock is reduced.

The invention provides a traditional Chinese medicinal composition for veterinary use. The traditional Chinese medicinal composition contains a Radix Astragali and Chinese angelica extract product which is prepared from 10-90 parts by weight of Radix Astragali and 10-90 parts by weight of Chinese angelica. The traditional Chinese medicinal composition for veterinary use can be used for preventing and curing livestock viral diseases and has characteristics of high efficiency and low toxicity. The traditional Chinese medicinal composition for veterinary use can be combined with a vaccine for livestock, so as to raise the immune effect of the vaccine. Through the combined application method, treatment effect also can be enhanced. The traditional Chinese medicinal composition for veterinary use has advantages of simple preparation method and low cost. The prepared preparation product requires small dose and is convenient to store, carry and use.

The invention discloses a method and a kit for performing quick co-detection on anti-Sp (Streptococcus pneumoniae) IgM (Immunoglobulin M) and IgG (Immunoglobulin G) antibodies based on magnetic separation and multi-color quantum dot labeling. The kit consists of anti-Sp antibody capturing nano magnetic beads with an anti-Sp IgM and IgG antibody gathering function, anti-human IgM and IgG antibody nano probes labeled by multi-color quantum dots, quality control substances and a PBST buffering solution, wherein the quality control substance comprise a positive quality control substance and a negative quality control substance; the positive quality control substance is serum, in which anti-Sp IgM and IgG antibodies of Sp infected people are respectively positive; the negative quality control substance is serum, in which anti-Sp IgM and IgG are respectively negative. The kit and the method have the advantages of simplicity, quickness and high sensitivity, and can be used for carrying out synchronous detection on the anti-Sp IgM and IgG antibodies.