Magnetic separation and multicolor quantum dot marking-based method and kit for quickly detecting anti-human chlamydia pneumoniae IgM and IgG antibodies simultaneously

Abstract

The invention discloses a magnetic separation and multicolor quantum dot marking-based method and a kit for quickly detecting anti-human chlamydia pneumoniae IgM and IgG antibodies simultaneously. The kit is composed of nanometer magnetic anti-human chlamydia pneumoniae antibody capturing beads, a multicolor quantum dot marked anti-human IgM antibody nanometer probe, a multicolor quantum dot marked anti-human IgG antibody nanometer probe, quality control products and a PBST buffer solution, wherein the quality control products comprise positive quality control products and negative quality control products; the positive quality control products are positive anti-human chlamydia pneumoniae IgM and IgG antibodies of a human chlamydia pneumoniae infected person and are positive serums, and the negative quality control products are negative anti-human chlamydia pneumoniae IgM and IgG and are negative human serums. The method has the advantages of simplicity, quickness and high sensitivity, and can detect the anti-human chlamydia pneumoniae IgM and IgG antibodies simultaneously.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a detection method and detection kit for rapid co-detection of anti-human pneumoniae IgM and IgG antibodies based on magnetic separation and multicolor quantum dot labeling, and the preparation and use of the detection kit method. Background technique [0002] Chlamydia pneumoniae (Cpn), as an important respiratory pathogenic microorganism of the genus Chlamydia, was officially named by Grayston et al. in the mid-1980s, and has been extensively studied by researchers in the subsequent time. It is only known that humans are the host of the pathogen, and the infection may be transmitted from person to person through respiratory secretions. Children under the age of 5 are rarely infected, and children over the age of 8 and young people are susceptible to infection, especially in places where people gather, such as families, schools, and barracks. Serological epidemiologic...

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Problems solved by technology

The specificity and sensitivity of the cold agglutination test are the lowest, so its clinical diagnostic value is not great; although the gold-labeled immunoblot method and the gold-labeled immunochromatography method are simple and quick to operate, their sensitivity is slightly low, and they are not suitable for those with low antibody levels. Patients may miss the diagnosis; the preparation and operation of gelatin agglutination test reagents are cumbersome and require professional operators

Enzyme-linked immunosorbent assay has the advantages of specificity and sensitivity, and has been used to check various antibodies or antigens. However, this method has the following problems: (1) There are many steps in each batch of tests, such as adding samples, warming baths, and washing plates. It is cumbersome and takes a long time (2-4 hours in total); (2) The color components A and B need to be added separately during the detection, and the reading must be completed within the specified time, which increases labor intensity and operation to a certain extent. Possibility of mistakes; (3) This method cannot achieve simultaneous detection of IgM and IgG antibodies

Although individual detection of IgM and IgG antibodies and comprehensive analysis of the test results have no effect on the diagnosis of the disease, the operation process is cumbersome, the detection is not convenient and fast, and the cost of detection will increase compared with the joint detection

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