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Inactivated lactic acid bacteria vaccine adjuvant

A technology for inactivating lactic acid bacteria and vaccine adjuvants, which is applied in the field of inactivated lactic acid bacteria vaccine adjuvants, and can solve the problems of high safety, efficacy to be improved, and high cost

Active Publication Date: 2018-03-16
WEIFANG HUAYING BIOTECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After being inoculated into the body, an "antigen library" can be formed, and the antigen can be released slowly, which fully prolongs the action time of the antigen, and can also promote the response of macrophages at the injection site. However, aluminum salt adjuvants have certain defects. For mild local reactions, granulomas may form
(2) Freund's adjuvant: Freund's adjuvant is a kind of oil-containing adjuvant, which contains mineral oil and inactivated Mycobacterium tuberculosis and other ingredients. It has a strong activation effect on humoral immunity and cellular immunity, but also has a strong side effects, especially severe inflammation and ulceration at the injection site when given subcutaneously
[0003] There are many types of adjuvants above, but their characteristics are different. For example, some adjuvants are simple to make, but have certain side effects, such as aluminum salt adjuvant and Freund’s adjuvant; some adjuvants are more complicated to prepare and cost higher. Such as cytokine adjuvants and adjuvants derived from microorganisms; some have higher safety, and their effectiveness may need to be improved, such as natural source immune adjuvants, etc.

Method used

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  • Inactivated lactic acid bacteria vaccine adjuvant
  • Inactivated lactic acid bacteria vaccine adjuvant
  • Inactivated lactic acid bacteria vaccine adjuvant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Inoculate Enterococcus faecium (purchased from China Industrial Microorganism Culture Collection and Management Center, Latin name: Enterococcusfaecium, preservation number: CICC 6049) in MRS medium, cultivate in an incubator at 37°C for 24 hours, then centrifuge at 3000 rpm for 5 minutes, remove Keep the precipitate in the upper layer culture solution, add sterile normal saline to wash the precipitate, centrifuge for 5 minutes, repeat the washing 3 times, add sterile normal saline, and mix with the precipitate. Get a certain amount of Enterococcus faecium physiological salt suspension solution, measure its OD value at the 690nm place of the spectrophotometer, when the OD value of the final concentration diluted with sterile physiological saline is 0.38, the Enterococcus faecium of such diluted concentration Physiological saline solution is used as 1 times (1×) concentration, and it can be determined that each ml of bacterial suspension at this concentration contains ~10...

Embodiment 2

[0024] Dilute the above-prepared inactivated Enterococcus faecium suspension to a concentration of 10×, and detect the effect of inactivated Enterococcus faecium as an adjuvant on the antibody levels of Newcastle disease and avian influenza H9 subtypes after chickens were inoculated with Xinliu dual inactivated vaccine . Twenty white-feathered broilers were randomly divided into two groups, namely the control group and the inactivated Enterococcus faecium adjuvant group, with 10 chickens in each group. Vaccine immunization was carried out at the age of 7 days. When the control group was vaccinated, 20 mL of sterile saline was added to 30 mL of Xinliu double inactivated oil seedlings and mixed well, and 0.5 mL was injected subcutaneously into the neck of each chick; When the coccal adjuvant group was vaccinated, 20 mL of inactivated Enterococcus faecium suspension at a concentration of 10× was added to 30 mL of Xinliu double inactivated oil seedlings, mixed evenly, and 0.5 mL w...

Embodiment 3

[0026] Lactobacillus plantarum subsp. plantarum (purchased from China Industrial Microorganism Culture Collection and Management Center, Latin name: Lactobacillus plantarum subsp.Plantarum, preservation number: CICC 6240) was prepared according to the method described in Example 1 into a 10× concentration of inactivated plant Lactobacillus subsp. plantarum vaccine adjuvant. To detect the effect of inactivated Lactobacillus plantarum subsp. plantarum as an adjuvant on the antibody level of mice inoculated with classical swine fever vaccine. Twenty clean-grade Kunming mice weighing 18-22 g were randomly divided into two groups, namely the control group and the inactivated Lactobacillus plantarum subsp. plantar adjuvant group, with 10 mice in each group, half male and half male. When the mice in the control group were inoculated with the live CSF vaccine, an equal volume of sterile saline was added to the live CSF vaccine; 100 μL was injected intramuscularly on the outer side of ...

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Abstract

The invention relates to an inactivated lactic acid bacteria vaccine adjuvant, which belongs to the field of a biological medicine. A main component of the adjuvant is inactivated lactic acid bacteria, and the adjuvant is prepared by inactivating the live lactic acid bacteria through a conventional method. The inactivated lactic acid bacteria can be taken as various vaccine adjuvant for human or animals, used for enhancing vaccine immunization effect, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to an inactivated lactic acid bacteria vaccine adjuvant. Background technique [0002] An adjuvant is a non-specific immune enhancer whose main function is to enhance the immune system's immune response to antigens or change the type of immune response. The most widespread use of adjuvants is to increase the antibody level of human and livestock vaccines to prevent infectious diseases. Adjuvants commonly used at present include the following types: (1) aluminum salt adjuvants: a class of inorganic salt adjuvants containing aluminum ions, such as aluminum hydroxide gel and alum. As an adsorbent, this type of adjuvant can strongly adsorb protein antigens from the solution and form a precipitate. After being inoculated into the body, an "antigen library" can be formed, and the antigen can be released slowly, which fully prolongs the action time of the antigen, and can also promote the resp...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61P37/04
CPCA61K39/39A61K2039/55594
Inventor 张勇石有斐
Owner WEIFANG HUAYING BIOTECH CO LTD
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