175results about How to "Fight infection" patented technology

The invention discloses spicy hot pot seasoning and a preparation method of the spicy hot pot seasoning. The spicy hot pot seasoning comprises the following raw materials: rapeseed oil, beef tallow, watercress, dried chilli, glutinous rice cake pepper, green prickleyash, fermented glutinous rice, broken rice sprouts, lobster sauce, amomun kravak pierre ex gagnep, medlar, lily, cumin, white lotus and the like. The preparation method comprises the steps of: heating up a pot, adding the rapeseed oil and the beef tallow, and putting in the dried chilli, the green prickleyash, ginger, garlic and green onions when bubbles do not exist for stir-frying; adding the glutinous rice cake pepper, the watercress, the broken rice sprouts and the lobster sauce for stir-frying at medium baking temperature; adding anise, kaempferiae, cinnamon, fennel, myrcia, lithospermum, tsaoko amomum fruit, clove, fructus amomi, the amomun kravak pierre ex gagnep, the cumin and the white lotus for stir-frying by using soft fire; and adding chicken essence, monosodium glutamate, salt, rock candy, the fermented glutinous rice, the medlar and the lily into the pot for stirring, turning off the heat and dishing up. The spicy hot pot seasoning and the preparation method use the mixed oil of the rapeseed oil and the beef tallow, and add the ingredients such as the cumin, the amomun kravak pierre ex gagnep, the medlar and the white lotus so as to effectively improve the adverse reactions such as parched mouth and scorched tongue, eye conjunctiva hyperemia and gastrointestinal discomfort after eating, and the spicy hot pot seasoning is mellow in taste, spicy but not dry, pungent but not strong, healthy and nutritional.

The invention relates to a water soluble body lubricant with natural antibacterial components and with pH value consistence with pH value of vagina, which is characterized by comprising the following components in parts by weight: 10-30 parts of glycerol, 1-5 parts of 1,2-propylene glycol, 0.1-3 parts of natural antibacterial components, 1-10 parts of pH regulators, 0.1-2 parts of tackifiers, 0.1-2 parts of hyaluronic acid and 48-88 parts of deionized water. The water soluble body lubricant can be used as the lubricant for sexual life, the lubricant on condoms or the external bactericide for the female to prevent and treat bacterial infection in the vagina every day, is temperate in property, has pH value consistent with the vaginal environment, has broad spectrum antibacterial effect, does not change the vaginal flora structure or result in drug resistance of bacteria, does not contain chemical preservatives, is safer to use and has certain effects on moisturizing, protecting and repairing the private parts.

Health function-improving agents, such as an immunological function-improving agent, an antioxidation function-improving agent, a exercise function-improving agent and a liver function-improving agent, comprising, as an effective ingredient, a δ-amino acid, a derivative thereof or a salt thereof

The present invention concerns a suture and clip material for surgical purposes which at least in parts comprises a biodegradable magnesium alloy with the following proportions by weight of the alloy components: rare earth metals between 2.0 and 4.0% by weight, yttrium between 3.5 and 4.5% by weight, zirconium between 0.3 and 1.0% by weight, no aluminum or at most aluminum <0.01% by weight, and balance <0.5% by weight, in particular <0.3% by weight, wherein magnesium occupies the proportion by weight that remains to 100% by weight in the alloy.

Pharmacologically and biologically active compositions containing carotenoids, in combination with micro and macro nutrients, a process for their preparation from carrots and their use in formulations for health care and nutrition applications. The process includes sequentially treating carrot juice with a carboxylic acid and a saccharide to obtain a carotenoid fraction rich in micro and macro nutrients in proportions compatible with those originally found in the natural state. A method of treating retenoid deficient states and immunomodulation is also disclosed using the composition.

The present invention relates to compositions, kits and methods for treating skin at sites of concerns such as infectious lesions and / or points of insertion of an insertable medical device to prevent contamination such as infections or protein absorption that may interfere with the function of the device. The composition can be a topical skin treatment composition that includes polyethylene glycol (PEG), triclosan, one or more of a salicylate, bronopol, and an acrylic emulsion, and, optionally, a solvent.

The present invention relates to the field of controlling pests, such as insects, using a virus to express pest genes in hosts. More specifically, the present invention relates to a method for rapidly screening for pest genes which can lead to mortality of the pest when the pest has ingested host tissues expressing virus-linked pest gene sequences. The present invention also relates to a method for controlling pests by viral expression of target pest sequences to modify endogenous expression of pest genes in cells or tissues of the pest.

The invention relates to engineered matrices comprising collagen fibrils with specific characteristics, including, but not limited to, a specific fibril area fraction (i.e., density) and / or a specific elastic or linear modulus (i.e., stiffness). The invention also relates to methods of preparation and use of the matrices.

The invention discloses a recombinant cell line for stable expression of porcine epidemic diarrhea virus S1 protein, a vaccine and an application. The recombinant cell line is constructed by transfecting HEK-293T cells by virtue of recombinant plasmid which is constructed by carrying a target gene on a lentiviral vector, and then transfecting the HEK-293T cells by virtue of generated high-titer virus particles; and the recombinant cell line, which can achieve stable expression, can still keep an excellent protein expression level after several passages. The recombinant cell line for stable expression of the porcine epidemic diarrhea virus S1 protein provided by the invention has the characteristics of being easy for culture, rapid in proliferation, unlimited in expansion, stable in property and high in protein expression amount; and when the vaccine, which is prepared from the expression protein and adjuvants, is used for immunizing pigs, the generation of a high-titer porcine epidemicdiarrhea virus neutralizing antibody can be induced from animal bodies, and the piglets (the pigs) can resist strong attack of porcine epidemic diarrhea viruses.

The present invention provides adapter-directed display systems for expressing exogenous polypeptide within a host cell and / or displaying the exogenous polypeptide on the outer surface of a genetic package. This subject systems are particularly useful for displaying a genetically diverse repertoire of monomeric and multimeric polypeptides. The invention also provides both expression and helper vectors and kits containing components of the subject display systems. Also provided are genetic packages displaying the exogenous polypeptides of particular interest. Further provided by the invention are methods of using the subject display systems.

The invention relates to a biological lysozyme compound preparation and a preparation method thereof. The preparation method comprises the following steps: dissolving lysozyme, amino acid, proteolytic enzyme, lysostaphin and peroxidase in water according to a certain proportion, transferring the mixed solution into a mixing tank, adding sorbitol, hydrated silica, aluminium hydroxide, glycerol, sodium lauroyl sarcosine, edible essence, cellulose gum, carrageenan, hydroxyethyl cellulose, sodium phytate, saccharin sodium, strontium chloride, trisodium phosphate, borneols, sodium borate, cinnabar, natrii sulfas exsiccatus and allantoin according to corresponding proportion, reacting for 47-49 h under a constant temperature condition of 38-42 DEG C to prepare the activated biological lysozyme compound preparation finished product. The biological lysozyme compound preparation belongs to a novel non-hormonic and non-antibiotic protein function preparation, has extremely great capacities of bacteriolyzing, reducing inflammation and enhancing immunity, and various effects of resisting various infections and promoting rehabilitation.

The invention discloses a polypeptide, an immunogenicity conjugate and an influenza vaccine, and relates to the bio-technical field. The polypeptide is any one of a), b) or c): a) a polypeptide with the amino acid sequence as shown in SEQ ID NO.1; b) fused protein obtained by connecting a label to the end N and / or the end C of the polypeptide with the amino acid sequence as shown in SEQ ID NO.1; c) a polypeptide which is obtained by substitution and / or deletion and / or adding of one or several amino acid residues for the amino acid sequence as shown in SEQ ID NO.1 and has the same function. The polypeptide can be used for preventing or treating influenza virus, and can be used as an active component for preparing a medicine for preventing or treating the influenza virus. The immunogenicity conjugate disclosed by the invention can improve the immunogenicity and the immune effect on an antigen at a conformational dependency site.

The invention discloses a TK, ge and gT gene deletion strain of a porcine pseudorabies virus (PRV) variant ZJ01 and application of the TK / ge / gT gene deletion strain. The strain is the TK / ge / gT gene deletion strain ZJ01delta TK / gE / gI (ZJ01R in short) of the PRV ZJ01 strain. According to the in-vitro passage test results, gE / gI gene deletion strains ZJ01delta gE / gI of a recombinant virus ZJ01R and the PRV ZJ01 strain are consistent with cytopathic effect generated by a parental virus ZJ01, and virus titer is similar with the ZJ01 strain; a target gene deletion region of the recombinant virus ZJ01R continuously and stably exists without resilience mutation; according to the pig immunity and attacking protection test, after the recombinant virus ZJ01R is immune, a pig is free of clinical symptoms and pathological changes; after being immunized by 21 days, the pig can generate a PRV neutralizing antibody with relatively high level, and can resist challenging infection of a fatal PRV variant strain ZJ01.

The invention relates to preparation and application of an efficient cell inactivation vaccine against new duck reovirus disease. The preparation method comprises the following steps: an antigen preparation step: inoculating a virus NDRV-TH11 strain with immortalized duck embryo fibroblast to obtain cytotoxicity with 80% of cytopathic effect, and performing cryopreservation at -20 DEG C; and a vaccine preparation step: inactivating the obtained cytotoxicity and mixing with a homemade nano adjuvant and stirring uniformly to obtain the efficient cell inactivation vaccine against new duck reovirus disease. After the duck is immunized by the efficient cell inactivation vaccine against new duck reovirus disease prepared in the invention, the duck can effectively resist the attack of a virulent strain of the new duck reovirus.

The invention discloses an antiphagin L-phenylalanine producing strain as well as a breeding method and application thereof, belonging to the technical field of bioengineering. The breeding method comprises the steps of: inducing and recombining a host bacterium WSH-Z06 of escherichia coli with nitrosoguanidine (NTG), breeding a strain with phage (CCTCC M) resistance by using phage separated from WSH-Z06(pAP-B03)(CCTCC M2010009) fermentation broth as a sieve, and transforming a plasmid pAP-B03 containing a L-phenylalanine synthase gene to finally obtain the antiphagin L-phenylalanine producing strain WSH-BR42(pAP-B03)(CCTCC M 2010008). Compared with a starting strain, the antiphagin L-phenylalanine recombinant strain WSH-BR42(pAP-B03) has the resistance to the phage (CCTCC M) and suffers from no influence to the growth speed and acid forming capability to the strain body. In the invention, the problem that the L-phenylalanine fermentation production is constantly polluted by the phage is solved.

The invention discloses a recombinant baby hamster kidney cell line (BHK 21) capable of stably expressing encephalitis B virus PrM / M-E protein, and more particularly, the recombinant cell line capable of expressing the PrM / M-E protein is BHK-JEV-ME and is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the culture preservation number of CGMCC No. 5263. The invention also discloses a method for preparing the recombinant cell line and the application of the recombinant cell line in the preparation of encephalitis B prevention vaccine.

The invention discloses a facility cultivation technique for crop rotation of stropharia rugosoannulata and rice, and belongs to the technical field of edible mushroom cultivation. The facility cultivation technique includes the steps of fermentation of a culture material, construction of a mushroom shed, construction of a ridged bed, seeding, mycelium management and protection, mushroom cultivation and harvesting, and rice planting and harvesting. After the components of the culture material are subjected to composting and fermentation separately, layered laying is adopted, so that nutrient and oxygen supply in the development process of mycelia and fruiting bodies is ensured, the whole fruiting is uniform, the fruiting rate is stable, the total yield is high, the quality is good, the planting cycle is short, and the continuous cropping obstacles can be solved by crop rotation of the stropharia rugosoannulata and rice.

The invention relates to a streptococcus-pneumoniae-toxicity-reducing live vaccine used for preventing streptococcus-pneumoniae infection. The vaccine is the composition of streptococcus pneumoniae strains which contain streptococcus pneumoniae SPD-1672 and have gene function deficiency, can effectively prevent the streptococcus-pneumoniae infection, has strong safety and good protection effect, and is easy for large-scale production and potential for generalization.

The invention discloses a porcine reproductive and respiratory syndrome resisting Marc-145 cell line as well as a preparation method and application thereof. The porcine reproductive and respiratory syndrome resisting Marc-145 cell line is obtained by knocking off CD163 extracellular domain SRCR5 of the Marc-145 cell line. The invention further provides gRNA (guide Ribonucleic Acid) for splicing of a pair of shearing Marc-145 cell CD163 receptors SRCR5 and sequences are shown as SEQ ID No. 2 and SEQ ID No. 3 respectively. After an the fifth extracellular fifth structure domain SRCR5 of the Marc-145 cell surface CD163 receptor on the surface of a Marc-145 cell is deleted through a CRISPR / CAS9 (Clustered Regularly Interspaced Short Palindromic Repeats / CRISPR associated protein 9) gene editing technology, the obtained porcine reproductive and respiratory syndrome resisting Marc-145 cell line can completely resist PRRSV (Porcine Reproductive and Respiratory Syndrome virus) infection and resist high-pathogenicity virulent strain HP-PRRSV. Moreover, the CD163 receptor on the surface of the porcine reproductive and respiratory syndrome resisting Marc-145 cell line surface CD163 receptor is normally expressed and other biological functions are normal. The porcine reproductive and respiratory syndrome resisting Marc-145 cell line disclosed by the invention has important meanings for inresearching PRRSV pathogenesis and mining novel disease-resisting or susceptible genes; a novel model is provided for PRRSV researches and the porcine reproductive and respiratory syndrome resisting Marc-145 cell line has important guidance meanings on in screening and breeding of PRRSV-resisting pigs.

The invention relates to a probiotics-containing special enzyme for piglets and a preparation method of the special enzyme, and belongs to the field of feed enzyme preparations. The probiotics-containing special enzyme for the piglets comprises the following components in parts by weight: 5-20 parts of Chinese herbal medicine powder, 800-1200 parts of xylanase, 80-120 parts of cellulase, 250-300 parts of bacillus licheniformis culture, 15-25 parts of mannose, 600-1000 parts of protease and 50-80 parts of medium-temperature alpha-amylase, wherein the bacillus licheniformis culture is bacillus licheniformis beta-10-25; the preservation number of the bacillus licheniformis culture is CCTCCNO.M2013538. The preparation method of the probiotics-containing special enzyme for the piglets comprises the following steps: fermenting bacillus licheniformis, then filtering, concentrating and drying the bacillus licheniformis to obtain a culture, compounding the culture with other components. The complex enzyme is capable of improving the feed conversion rate, reducing the feed cost and improving preservation stability.

The invention discloses a method for brewing papaya rice wine. The preparation method comprises the following steps of: selecting fresh papaya, crushing, juicing, mixing, fermenting, filtering, performing post-fermentation, clarifying, ageing, and soaking fruit residues; and mixing, clarifying, filtering and canning. The nutrition and medicinal value of papaya are mixed, the drinking mouthfeel aroma and aroma coordination of the rice wine are improved, and the sourness and sweetness are moderate.

The invention belongs to the field of surgical dressing and specifically relates to a traditional Chinese medicine liquid dressing and a preparation method. The traditional Chinese medicine liquid dressing comprises the following components by weight percent: 1.0%-6.0% of film-forming material, 2.0%-4.0% of glycerinum, 0.5%-3.0% of menthol, 0.3%-1.0% of coptis chinensis extract, 1.0%-2.0% of honeysuckle extract and the balance of solvent. The traditional Chinese medicine liquid dressing provided by the invention is acquired according to the steps of mixing the above components in turn, uniformly stirring and filling. The traditional Chinese medicine liquid dressing provided by the invention has the advantages of simple component, short film-forming time, waterproof effect, good antibacterial effect, no drug-fast property and capability of boosting wound healing and shortening the wound healing time.

The invention discloses a process for manufacturing decavitamin nanoemulsion for livestock and poultry. The process comprises the steps of: smelting and stirring an oil phase material to uniform faint yellow transparent liquid; preparing an aqueous phase material into mixed liquid, adding the mixed liquid into the oil phase material for 2-5 times according to a certain liquid ratio, uniformly stirring, and regulating the pH value to obtain decavitamin initial emulsion; then performing nanometer granulation treatment in a nanometer homogenizer, filtering by a filter with an aperture of 0.11-0.22 micron, and performing aseptic package to obtain the decavitamin nanoemulsion for livestock and poultry. The decavitamin nanoemulsion prepared by using the process in the invention has good antiseptic property and isolates bacteria without preservative to achieve green and environment-friendly development of livestock and poultry industry, meanwhile, the decavitamin nanoemulsion has the beneficial effects of improving the digestion and absorption of livestock and poultry animals on nutrient substance, promoting the growth and development of the livestock and poultry animals, enhancing the immunity of the livestock and poultry animals, facilitating to improve the slaughtering rate of the livestock and poultry animals, reducing the breeding cost, shortening the breeding period and effectively preventing the injury of excessive antibiotics to the biologic chain.

The invention discloses cellulose enhanced glucan-based injectable antibacterial hydrogel dressing and a preparation method thereof. The hydrogel is obtained by crosslinking aminated glucan and aldehyde glucan at the body temperature of a human body, and modified cellulose short fiber and polypeptide are distributed in the hydrogel. The preparation method comprises the following steps: reacting the glucan with anhydride, and removing BOC to obtain glucan containing multi-amino; selectively oxidizing the glucan into the aldehyde glucan by an oxidizing agent; and preparing aqueous solutions fromthe glucan containing multi-amino and the aldehyde glucan correspondingly, mixing the aqueous solutions, adding modified cellulose short fiber and solid-phase synthesized polypeptide, and preparing the cellulose enhanced glucan-based injectable antibacterial hydrogel dressing under the condition of the body temperature of the human body. The cellulose enhanced glucan-based injectable antibacterial hydrogel dressing has high mechanical property, has excellent biocompatibility and antibacterial property, has excellent compression-resistant and elastic properties, can promote rapid healing of wound and is suitable for wound treatment and repair of chronic skin ulcer with different depth.

The invention discloses a polypeptide, an immunogenicity conjugate and an influenza vaccine, and relates to the bio-technical field. The polypeptide is any one of a), b) or c): a) a polypeptide with the amino acid sequence as shown in SEQ ID NO.1; b) fused protein obtained by connecting a label to the end N and / or the end C of the polypeptide with the amino acid sequence as shown in SEQ ID NO.1; c) a polypeptide which is obtained by substitution and / or deletion and / or adding of one or several amino acid residues for the amino acid sequence as shown in SEQ ID NO.1 and has the same function. The polypeptide can be used for preventing or treating influenza virus, and can be used as an active component for preparing a medicine for preventing or treating the influenza virus. The immunogenicity conjugate disclosed by the invention can improve the immunogenicity and the immune effect on an antigen at a conformational dependency site.

The invention discloses compound feed for improving structure and function of piglet digestive tracts, which comprises, in weight percentage, 50-65% of corn, 0-15% of wheat, 10-25% of soybean meal, 5-8% of wheat bran, 3.5-4.5% of premix, 0.05-0.15% of mycotoxin adsorbent, 0.01-0.03% of microecological agent, 0.01-0.015% of plant extract, 0.008-0.015% of organic acid, and 0.01-0.02% of sodium butyrate. Intestinal tracts of piglets fed with the compound feed are healthier, the piglets suffer fewer diarrheas, productivity is improved, and the service effect of the compound feed is more evident than that of antibiotics.

In general, this invention relates to nucleic acid and amino acid sequences involved in molting and the use of these sequences as targets for the development of compounds that disrupt Ecdysozoan molting, and are useful as insecticides, nematicides, and anti-parasitic agents.

The invention relates to a feed additive special for a rex rabbit. The additive comprises the components by weight percent: 5-8% of cyrtomium fortunei, 3-5% of semen cuscutae, 15-20% of folium isatidis, 6-9% of sweet wormwood, 10-15% of astragalus mongholicus, 2-4% of coptis chinensis, 4-8% of valerian, 10-20% of unsaturated fatty acid composition and the balance of carriers. The unsaturated fatty acid composition is formed by mixing 50 parts of linoleic acid, 20 parts of arachidonic acid, 15 parts of linolenic acid and 0.005 part of ethyoxyl quinoline. 0.5-3% of a feed additive special for the rex rabbit provided by the invention is added into and uniformly mixed in common marketed feed for the rex rabbit. The feed additive special for the rex rabbit can alleviate stress reaction in breeding of the rex rabbit, improve the immunity of the rex rabbit and accelerate growth of the rex rabbit.

The invention discloses digestive lactic acid bacteria composite powder which is prepared from raw materials including, by weight, 20-35 parts of inulin, 15-20 parts of sorbitol, 15-20 parts of integrated fruit and vegetable powder, 5-15 parts of maltodextrin, 5-15 parts of compound probiotics powder, 5-15 parts of West-Indian cherry fruit powder, 5-15 parts of composite-enzyme tri-beneficial bacteria, 3-10 parts of fructo-oligose and 3-10 parts of vitamin C. The invention further discloses a preparation technology of the product. The synergic effect of the components in the composite powder is achieved, a biological barrier is formed, invasion and infection of harmful bacteria is resisted, immunity is enhanced, detoxifying is promoted, digestion, absorption and metabolism of the intestinal tract is facilitated, nutrients and cholesterol are helpfully synthesized, and metabolism of fat, protein and saccharides is promoted; by the use of the product, gastrointestinal environment can be purified, internal organs can be protected, acidic condition of the human body can be adjusted into healthy alkaline condition, blood vessels can be purified, and triglyceride can be reduced; the product is suitable for most of the crowd like pregnant women and children, wide in application of the crowd and good in use effect.