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Preparation method and application of high-purity polysaccharopeptide

A Yunzhi glycopeptide, a high-purity technology, is applied in the preparation methods of peptides, chemical instruments and methods, medical preparations containing active ingredients, etc., and can solve the problem of no reports of livestock and poultry vaccine immune enhancers.

Active Publication Date: 2013-02-27
重庆优宝生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the current domestic and foreign literature, there are many studies on Yunzhi glycopeptide, and there are many reports on the extraction and application of Yunzhi glycopeptide, but there is a lack of a high-purity extraction and purification process for Yunzhi glycopeptide, and it has not been used as a Report on Immunity Booster for Livestock and Poultry Vaccines

Method used

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  • Preparation method and application of high-purity polysaccharopeptide
  • Preparation method and application of high-purity polysaccharopeptide
  • Preparation method and application of high-purity polysaccharopeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Extraction and Purification of Yunzhi Glycopeptide

[0021] Take Yunzhi glycopeptide coarse powder 1kg, add 6% (w / v) NaHCO according to the ratio of material to water 1:48 3 Aqueous solution, heated to 70-72°C, heat-preserved and leached for 2 hours, centrifuged at 5500rpm, the supernatant was taken, heated to 75°C, heat-preserved and concentrated 9 times, then added 6 times the volume of absolute ethanol, static at 1-3°C After 24 hours, centrifuge at 4500rpm, discard the supernatant, wash the precipitate twice with 95% ethanol, absolute ethanol, acetone, and ether (4000rpm, 5min), and dry to obtain 492.5g of semi-finished versicolor glycopeptide.

[0022] Take 500g of Yunzhi glycopeptide semi-finished product, add double distilled water to dissolve, then add 0.25 times the volume of organic solvent, the organic solvent is chloroform and n-butanol with a volume ratio of 5:1, vigorously shake and mix for 20 minutes, and centrifuge at 4500rpm for 10 minutes , t...

Embodiment 2

[0026] Example 2 Extraction and Purification of Yunzhi Glycopeptide

[0027] Take Yunzhi glycopeptide coarse powder 1kg, add 8% (w / v) NaHCO according to the ratio of material to water 1:40 3 Aqueous solution, heated to 70-72°C, heat-preserved and leached for 1.5h, centrifuged at 6000rpm, took the supernatant, heated to 78°C and concentrated 8 times, then added 5 times the volume of absolute ethanol, in the environment of 1-3°C After standing for 20 hours, centrifuge at 5000rpm, discard the supernatant, wash the precipitate twice with 95% ethanol, absolute ethanol, acetone, and ether (4000rpm, 5min), and dry to obtain 489.8g of semi-finished versicolor glycopeptide.

[0028] Take 500g of Yunzhi glycopeptide semi-finished product, add double distilled water to dissolve, then add 0.2 times the volume of organic solvent, the organic solvent is chloroform and n-butanol with a volume ratio of 5:1, shake vigorously for 15 minutes, and centrifuge at 4500rpm for 10 minutes , take the ...

Embodiment 3

[0032] Example 3 Extraction and Purification of Yunzhi Glycopeptide

[0033] Take Yunzhi glycopeptide coarse powder 1kg, add 7% (w / v) NaHCO according to the ratio of material to water 1:55 3 Aqueous solution, heated to 70-72°C, heat preservation and leaching for 2.5h, centrifuged at 5000rpm, take the supernatant, heat to 78°C, heat preservation and concentrate 10 times, then add 7 times the volume of absolute ethanol, in the environment of 1-3°C After standing for 30 hours, centrifuge at 4500rpm, discard the supernatant, wash the precipitate twice with 95% ethanol, absolute ethanol, acetone, and ether (4000rpm, 5min), and dry to obtain 485.0g of semi-finished versicolor glycopeptide.

[0034] Take 500g of Yunzhi glycopeptide semi-finished product, add double distilled water to dissolve, then add 0.3 times the volume of organic solvent, the organic solvent is chloroform and n-butanol with a volume ratio of 5:1, vigorously shake and mix for 25 minutes, and centrifuge at 5000rpm fo...

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Abstract

The invention relates to a preparation method and application of high-purity polysaccharopeptide. The preparation method comprises extracting and purifying polysaccharopeptide coarse powder and obtaining the polysaccharopeptide with high polysaccharide content and high protein level. The polysaccharopeptide can improve antibody levels of chickens, accordingly improve immunity of the chickens, be used for preparing human medicines or beast medicines with immunity or / and physiological accommodation activity and particularly serve as vaccine immunopotentiator of beasts and birds.

Description

technical field [0001] The invention relates to the technical field of production of veterinary or human medicines, in particular to a preparation method and pharmaceutical application of high-purity versicolor glycopeptide. Background technique [0002] Bacterial and viral infectious diseases are the enemies that endanger the production of animal husbandry. With the increasing intensification of animal husbandry, the prevention of infectious diseases becomes more important. For viral diseases, how to choose the appropriate drug treatment has always been a headache for farmers. At the same time, the drug treatment of viral diseases is difficult to be effective, and immunization must be carried out to prevent it. However, in the production process, animal immunity failures often occur, such as improper vaccine transportation, storage, unreasonable immunization procedures, wrong vaccination methods, inaccurate doses, such as too much or too little inoculation dose, etc., resul...

Claims

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Application Information

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IPC IPC(8): C07K1/14A61K39/39
Inventor 任丽孟怀旺
Owner 重庆优宝生物技术股份有限公司
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