The invention provides a porcine epidemic diarrhea virus strain SD10 which is preserved in General Microbiology Center of China Committee for Culture Collection of Microorganisms, School of Chinese Academy of Sciences Committee, at Beichen West Road, No. 1 yard, No. 3, Chaoyang District, Beijing, on November 8, 2013, and the preservation number is CGMCC No. 8503. Compared with the conventional viruses, the porcine epidemic diarrhea virus strain SD10 screened by the invention is low in toxicity, and good in immunogenicity; the prepared vaccine can quick generate antibodies after immunizing; the generated antibodies are high in titer and long in duration; the vaccine is long in storage life, and small in immunity dose, and can enable piglets of a pregnant sow to get better ectophylaxination if being injected to the pregnant sow before mating, so that the piglets can generate strong immune and can resist virulent attacks, and the piglet survival is improved.