Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Triple inactivated vaccine for rabbit viral hemorrhagic disease, pasteurellosis and bordetella disease and preparation method of vaccine

A technology for rabbit viral hemorrhage and bordetella disease, applied in the direction of virus/phage, biochemical equipment and methods, virus, etc.

Active Publication Date: 2019-09-06
QILU ANIMAL HEALTH PROD
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there is no vaccine related to the simultaneous prevention and control of rabbit viral hemorrhagic disease, pasteurellosis multocida, and bordella bronchiseptica

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Triple inactivated vaccine for rabbit viral hemorrhagic disease, pasteurellosis and bordetella disease and preparation method of vaccine
  • Triple inactivated vaccine for rabbit viral hemorrhagic disease, pasteurellosis and bordetella disease and preparation method of vaccine
  • Triple inactivated vaccine for rabbit viral hemorrhagic disease, pasteurellosis and bordetella disease and preparation method of vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Embodiment 1 - construction of recombinant baculovirus VP60 strain

[0075] 1. Construction of transfer vector pVL-HBM

[0076] (1) HBM gene amplification HBM gene amplification uses pMD19-HBM as a template, and HBM-F and HBM-R as primers for PCR amplification ( figure 1 ).

[0077] HBM-F (sequence 1): 5'-agcggatcca aaccatgaaa ttc-3'(BamHI) 23

[0078] HBM-R (sequence 2): 5'-ataagatctg catgcggtac ccc-3'(PstⅠ)23

[0079] (2) Enzyme digestion The correctly identified PCR product was recovered by a DNA gel recovery kit, the pVL1393 plasmid and the purified HBM gene PCR product were digested with BamHI / PstI at 37°C for 3 hours, and the digested product was subjected to gel electrophoresis. Gel Recovery and Purification Kit for purification and recovery.

[0080](3) Ligation and transformation The digested products were subjected to gel electrophoresis, and purified and recovered using a gel recovery and purification kit. The digested and purified pVL1393 plasmid and th...

Embodiment 2

[0104] Embodiment 2——vaccine preparation

[0105] 1. Preparation of semi-finished product of recombinant baculovirus VP60 strain

[0106] (1) Propagation of virus seeds Inoculate the well-grown Sf9 cells with the basic virus seeds of the recombinant baculovirus VP60 strain, culture at 27°C for 72-96 hours, harvest the virus liquid, pass it on for 2 generations, harvest the virus liquid of the fourth generation, and use it as a production virus kind.

[0107] (2) Preparation of cells for production

[0108] 1) Cell recovery Take the frozen Sf9 cells out of the liquid nitrogen, put them into warm water at 37°C and shake quickly until the frozen solution is completely dissolved, transfer the Sf9 cell suspension to a centrifuge tube, centrifuge at 620r / min for 5min, discard For the supernatant, resuspend the cells with SF-SFM medium, place them in a Erlenmeyer flask with a suitable capacity, and culture them on a shaker at 27°C with a rotation speed of 100-105r / min.

[0109] 2)...

Embodiment 3

[0134] Embodiment 3 - the inspection of vaccine

[0135] 1. Properties After standing still, the upper layer is a clear liquid, and the lower layer has a small amount of precipitation. After shaking, it becomes a uniform suspension.

[0136] 2. Filling inspection and sterility inspection are carried out according to the appendix of the current "Chinese Veterinary Pharmacopoeia", and all of them meet the regulations.

[0137] 3. Safety inspection 3 batches of laboratory products were subcutaneously immunized healthy susceptible rabbits around 45 days old, 2.0ml / piece, and observed continuously for 10 days. The results showed that all the experimental rabbits were healthy and alive, and their spirit, diet, feces, and body temperature were all normal. ; There was no swelling or necrosis at the inoculation site, and no other abnormal clinical symptoms appeared. The specific results are shown in Table 1.

[0138] Table 1 Safety test results of 3 batches of laboratory products

...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a triple inactivated vaccine for rabbit viral hemorrhagic disease, pasteurellosis and bordetella disease and a preparation method of the vaccine. The effective components of the vaccine comprise VP60 protein antigen of rabbit viral hemorrhagic disease virus, inactivated pasteurella multocida QLT-1 strain and bordetella bronchiseptica JN01 strain antigen. The triple inactivated vaccine has fast generation of protective antibody after immunization and high immune attack protection rate, with the attack protection rate for rabbit viral hemorrhagic disease virus AV-34 strain, pasteurella multocida QLT-1 strain and bordetella bronchiseptica JN01 strain all reaching above 80%. The results show that the vaccine is safe and reliable, and can be used for preventing the occurrence of rabbit viral hemorrhagic disease (rabbit plague), rabbit pasteurella multocida disease (type A) and rabbit bronchisepticaemia Bordetella disease.

Description

technical field [0001] The invention relates to a triple inactivated vaccine of rabbit viral hemorrhagic disease, pasteurellosis and bordetella disease and a preparation method thereof. It belongs to the field of veterinary biological products. Background technique [0002] Rabbit viral hemorrhagic disease (viral haemorrhagic disease of rabbits) is an acute, septic, highly contagious, and highly lethal infectious disease of rabbits caused by rabbit haemorrhagic disease virus (RHDV) infection. Disease, also known as rabbit hemorrhagic pneumonia and rabbit hemorrhagic disease virus, commonly known as rabbit plague, is an infectious disease characterized by edema, congestion and bleeding in the parenchymal organs of the whole body. Rabbit viral hemorrhagic disease is a worldwide epidemic distribution, with a high morbidity and fatality rate, up to 100%. It is one of the common and frequently-occurring diseases of rabbits. In 1989, the disease was officially listed as a B-type ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K39/23A61P31/20A61K39/102A61K39/02A61P31/04
CPCA61K39/12A61P31/20A61K39/102A61K39/0208A61P31/04A61K2039/70A61K2039/552A61K2039/5252A61K2039/521C12N2750/14334Y02A50/30
Inventor 陈辉张小军宋晓飞葛平萍秦绪伟张娣李金波贾爱琴王蕾徐龙涛
Owner QILU ANIMAL HEALTH PROD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com