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Porcine epidemic diarrhea virus and application thereof

A porcine epidemic diarrhea and virus technology, applied in antiviral agents, antiviral immunoglobulins, inactivation/attenuation, etc., can solve the problems of poor clinical application effect, achieve resistance to attack, small immune dose, and immunogenicity Good results

Active Publication Date: 2014-05-28
YEBIO BIOENG OF QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, foreign countries are mainly attenuated vaccines from South Korea, and domestically mainly are porcine transmissible gastroenteritis and epidemic diarrhea inactivated vaccines developed by Harbin Animal Research Institute, but the clinical application effect is relatively poor

Method used

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  • Porcine epidemic diarrhea virus and application thereof
  • Porcine epidemic diarrhea virus and application thereof
  • Porcine epidemic diarrhea virus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Example 1: Screening of porcine epidemic diarrhea virus SD10 strain

[0010] 1.1 Isolation and Identification of Porcine Epidemic Diarrhea Virus SD10 Strain During the epidemiological investigation, a strain of porcine epidemic diarrhea virus (named SD10A1 strain) was isolated from the contents of the small intestine of a pig farm with diarrhea in Laixi, Shandong Province. ), porcine epidemic diarrhea virus RT-PCR test was positive, blind transmission to the 8th generation of cytopathy, the 16th generation of regular and stable cytopathy, virus content ≥ 10 5.0 TCID 50 / 0.1ml, can be neutralized by anti-porcine epidemic diarrhea virus-specific serum. When passed to the 121st generation, the passaged virus is safe, pure, and free of bacteria, mold, and mycoplasma for 3-5 day-old healthy piglets that have not fed colostrum and exogenous viruses (classic swine fever, porcine parvovirus, bovine viral diarrhea virus, porcine reproductive and respiratory syndrome, porcine fo...

Embodiment 2

[0036] The safety test of embodiment 2 porcine epidemic diarrhea live vaccine

[0037] 1. Materials and methods

[0038] 1.1 Single dose test

[0039] 1.1.1 Single-dose test on pregnant sows Take 6 pregnant sows that were negative for porcine epidemic diarrhea neutralizing antibodies and antigens 4 to 6 weeks before delivery, and randomly divided them into 2 groups, 3 in each group, the first group The 201001 batches of porcine epidemic diarrhea live vaccines were intramuscularly injected with 1 part per head, and the second group was not injected as the control group, and the sows were observed until the sows gave birth.

[0040] 1.1.2 Single-dose test on piglets Take 12 1-day-old piglets and randomly divide them into 2 groups, 6 pigs in each group. The first group takes 1 batch of 201001 porcine epidemic diarrhea live vaccine orally, and the second group does not Oral as a control group, observed for 14 days.

[0041] 1.2 Single-dose repeated test

[0042] 1.2.1 Repeated...

Embodiment 3

[0051] Efficacy test of embodiment 3 porcine epidemic diarrhea live vaccine

[0052] 1. Active immunization test

[0053] 1.1 Materials and methods Eight sows were randomly divided into 2 groups with 4 pigs in each group. The first group was injected with 201,001 batches of porcine epidemic diarrhea live vaccine in the neck muscle, and the second group was not injected as a control. Group, after the sows gave birth, 14 piglets were taken respectively at the age of 3 days, 7 days, 14 days, and 21 days, and were randomly divided into 2 groups, with 7 pigs in each group. Clinical manifestations of piglets after challenge.

[0054] 1.2 Results The piglets produced by the immunized sows, after the 3-day-old challenge, 7 immunized piglets showed no abnormalities in lactation, spirit, and feces, and were all healthy and alive; after the 7 control piglets were challenged, 7 / 7 showed typical pig epidemics. Sexual diarrhea symptoms, 6 / 7 died. After the 7-day-old challenge, the immune...

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Abstract

The invention provides a porcine epidemic diarrhea virus strain SD10 which is preserved in General Microbiology Center of China Committee for Culture Collection of Microorganisms, School of Chinese Academy of Sciences Committee, at Beichen West Road, No. 1 yard, No. 3, Chaoyang District, Beijing, on November 8, 2013, and the preservation number is CGMCC No. 8503. Compared with the conventional viruses, the porcine epidemic diarrhea virus strain SD10 screened by the invention is low in toxicity, and good in immunogenicity; the prepared vaccine can quick generate antibodies after immunizing; the generated antibodies are high in titer and long in duration; the vaccine is long in storage life, and small in immunity dose, and can enable piglets of a pregnant sow to get better ectophylaxination if being injected to the pregnant sow before mating, so that the piglets can generate strong immune and can resist virulent attacks, and the piglet survival is improved.

Description

technical field [0001] The invention belongs to the field of veterinary biological products, in particular to a porcine epidemic diarrhea virus and its application. Background technique [0002] Porcine epidemic diarrhea (Porcine epidemic diarrhea, PED) is a highly contagious intestinal infectious disease of pigs caused by porcine epidemic diarrhea virus. It is characterized by vomiting, diarrhea and loss of appetite. The disease affects pigs of different ages. All are susceptible, especially suckling piglets are the most harmful, and it is a highly contagious infectious disease. The disease first appeared in the United Kingdom in 1971, and occurred successively in my country in the early 1980s. In recent years, the outbreak of porcine epidemic diarrhea in the whole country has caused huge economic losses to the breeding industry. There is currently no effective treatment for this disease infection, mainly based on vaccine immunization prevention. At present, foreign coun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/04C07K16/10A61K39/215A61P31/14C12R1/93
Inventor 陶晓珊刘蕾韩乃君申洪银邹桂荣邹敏胡潇范根成孙健
Owner YEBIO BIOENG OF QINGDAO
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