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A dual vaccine against porcine epidemic diarrhea and Escherichia coli

A porcine epidemic diarrhea and Escherichia coli technology, applied in the field of porcine epidemic diarrhea and Escherichia coli dual vaccine, can solve the problems of no effective vaccines for preventing porcine epidemic diarrhea and piglet yellow scour, and achieve Good passive immunity, small immune dose, long-lasting effect

Active Publication Date: 2016-03-30
YEBIO BIOENG OF QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no effective vaccine for preventing porcine epidemic diarrhea and piglet yellow scour in the market

Method used

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  • A dual vaccine against porcine epidemic diarrhea and Escherichia coli
  • A dual vaccine against porcine epidemic diarrhea and Escherichia coli
  • A dual vaccine against porcine epidemic diarrhea and Escherichia coli

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Screening of porcine epidemic diarrhea virus SD10 strain

[0022] 1.1 Isolation and Identification of Porcine Epidemic Diarrhea Virus SD10 Strain During the epidemiological investigation, a strain of porcine epidemic diarrhea virus (named SD10A1 strain) was isolated from the contents of the small intestine of a pig farm with diarrhea in Laixi, Shandong Province. ), porcine epidemic diarrhea virus RT-PCR test was positive, blind transmission to the 8th generation of cytopathy, the 16th generation of regular and stable cytopathy, virus content ≥ 10 5.0 TCID 50 / 0.1ml, can be neutralized by anti-porcine epidemic diarrhea virus-specific serum. When passed to the 121st generation, the passaged virus is safe, pure, and free of bacteria, mold, and mycoplasma for 3-5 day-old healthy piglets that have not fed colostrum and exogenous viruses (classic swine fever, porcine parvovirus, bovine viral diarrhea virus, porcine reproductive and respiratory syndrome, porcine foo...

Embodiment 2

[0047] Preparation and inspection of porcine epidemic diarrhea and Escherichia coli dual inactivated vaccine of embodiment 2

[0048] 1. Preparation of semi-finished products

[0049] 1.1 Prepare Vero cells according to conventional methods. The growth medium is MEM containing 8% newborn bovine serum. When the cells grow into a single layer, they are inoculated with poison;

[0050] 1.2 Take porcine epidemic diarrhea virus SD10 strain virus seeds (≥10 5.0 TCID 50 / 0.1ml) Inoculate a monolayer of well-growing Vero cells at 1%, place at 37°C for 1.5 hours, add MEM maintenance solution containing 2% newborn bovine serum to continue culturing;

[0051] 1.3 After inoculation, observe twice a day, and record the cell lesions. When the cell lesions reach more than 75%, harvest the cells, and freeze and thaw them repeatedly 3 times;

[0052] 1.4 Take the virus liquid after freezing and thawing to measure the virus content, and the virus content per 0.1ml is not less than 10 5.0 TC...

Embodiment 3

[0071] The safety test of embodiment 3 porcine epidemic diarrhea, Escherichia coli dual inactivated vaccine

[0072] 1. Materials and Methods

[0073] 1.1 Single-dose test 6 pregnant sows aged 30-45 days before delivery were randomly divided into 2 groups with 3 heads in each group. The first group was intramuscularly injected with dual inactivated vaccine 2ml / head in the neck, and observed until the litter was born.

[0074] 1.2 Single-dose repeated test with 6 pregnant sows aged 30-45 days before delivery, randomly divided into 2 groups, 3 heads in each group, the first group was injected with dual inactivated vaccine 2ml / head in the neck muscle, and immunized 14 days after immunization The group was injected with the vaccine again, with the same batch number and dosage as before, and was observed until calving.

[0075] 1.3 An overdose test used 6 pregnant sows aged 30-45 days before delivery, and randomly divided them into 2 groups, 3 heads in each group. The first group ...

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Abstract

The invention provides a bivalent vaccine for preventing porcine epidemic diarrhea and Escherichia coli. The bivalent vaccine comprises an antigen and a vaccine adjuvant, wherein the antigen is a fimbriae antigen of porcine epidemic diarrhea bacterium and Escherichia coli K88, K99 and 987P conserved under CGMCC No. 8503. The bivalent vaccine for preventing porcine epidemic diarrhea and Escherichia coli has good immunogenicity, and can quickly produce antibody after immunity, the produced antibody has high titer and long preservation period and can maintain for long time, the immunizing dose is small, and the adjuvant selected is easily injected; the bivalent vaccine can be used for preventing two types of diseases, piglets given birth by pregnant sows which are inoculated with the vaccine before hybridization can obtain better passive immunity and have strong immunity against virulent strains, and therefore the survival rate of the piglets is improved.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a porcine epidemic diarrhea-Escherichia coli dual vaccine. Background technique [0002] Porcine epidemic diarrhea (Porcineepidemicdiarrhea, PED) is a highly contagious intestinal infectious disease of pigs caused by porcine epidemic diarrhea virus. It is characterized by vomiting, diarrhea and loss of appetite. The disease is susceptible to pigs of different ages Infection, especially in suckling piglets, is the most serious and is a highly contagious infectious disease. The disease first appeared in the United Kingdom in 1971, and occurred successively in my country in the early 1980s. In recent years, the outbreak of porcine epidemic diarrhea in the whole country has caused huge economic losses to the breeding industry. There is currently no effective treatment for this disease infection, mainly based on vaccine immunization prevention. At...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/295A61K39/39A61P1/12A61P31/14A61P31/04
Inventor 刘蕾于晓云郭玉广申洪银陶晓珊邹桂荣邹敏胡潇范根成孙健
Owner YEBIO BIOENG OF QINGDAO
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