The invention discloses a soft-shelled turtle systemic septicemia spherical
virus inactivated vaccine and its preparation method, and is characterized in that the vaccine contains the inactivated soft-shelled turtle systemic septicemia spherical
virus with the collection number being CGMCCNo.5378. The preparation method comprises the following steps of:
cutting internal organ of sick soft-shelledturtles into pieces, adding into a TNE buffer, centrifuging, taking a supernatant, centrifuging, taking a white precipitate, suspending in the TNE buffer, centrifuging the precipitate, taking the supernatant, and centrifuging to obtain a white precipitate, namely the purified
virus; diluting the purified virus by the use of aseptic normal
saline until the final concentration of
viral protein reaches 0.5-1mg / ml, and adding 0.5% (final volume) of formalin for inactivation in a
thermostat water bath cauldron; and completely emulsifying inactivated virus and a complete Freund's
adjuvant accordingto the volume ratio of 1:1 to obtain the
inactivated vaccine. The soft-shelled turtle systemic septicemia spherical virus
inactivated vaccine proposed for the first time is safe and effective and hasstrong
immunity, and the preparation method is simple and easy to operate.