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Infectious bursal disease antigen-antibody complex as well as preparation and preparation method thereof

A technology of antigen antibody and chicken infectivity, applied in the direction of virus antigen components, antibodies, antiviral agents, etc., can solve the problems of stress, tolerance, risk of poisoning, etc., and achieve simple and fast preparation, safe and convenient use , fast immune effect

Active Publication Date: 2013-10-09
TIANJIN RINGPU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the production and use of related products, the inactivated whole virus has the risk of spreading the virus. At the same time, because chemical reagents such as formaldehyde are often introduced in the process of virus inactivation, it is easy to cause stress and tolerance problems in the immune body. Therefore, The introduction of new specific antigens is urgently needed in the preparation of antigen-antibody complexes

Method used

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  • Infectious bursal disease antigen-antibody complex as well as preparation and preparation method thereof
  • Infectious bursal disease antigen-antibody complex as well as preparation and preparation method thereof

Examples

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Effect test

Embodiment 1

[0028] Embodiment 1: chicken infectious bursal disease antigen-antibody complex

[0029] Chicken infectious bursal disease antigen-antibody complex is a mixture of antigen and antibody, in which the antigen is the chicken infectious bursal virus VP2 protein prepared by conventional biotechnology means, and the antibody is the inactivated chicken infectious bursal virus Egg yolk antibody to bursal virus inactivated vaccine.

Embodiment 2

[0030] Embodiment 2: the preparation of chicken infectious bursal virus VP2 protein

[0031]Using gene cloning technology, the VP2 gene of chicken infectious bursal virus is cloned into a prokaryotic expression vector, transformed into a prokaryotic expression host to induce expression, and purified by affinity chromatography to obtain VP2 protein. The specific method is: refer to GenBank and the VP2 co-conserved segment of super-virulent strains, classic virulent strains and attenuated strains of infectious bursal disease, use software Premier 5.0 to design and synthesize upstream and downstream primers for VP2 gene; extract infected chickens The bursa tissue of SPF chickens of infectious bursal disease supervirulent strain (vvIBDV) was ground and homogenized, and the total RNA of vvIBDV genome was extracted by conventional RNA extraction technology and reverse-transcribed into cDNA ; Using cDNA as a template, amplify the VP2 gene under the action of the above primers and rec...

Embodiment 3

[0032] Embodiment 3: the preparation of chicken infectious bursal virus egg yolk antibody IgY

[0033] Eggs produced by high-immune laying hens immunized with chicken infectious bursal disease inactivated vaccine for multiple times are collected, positive yolks are separated for acidification and extraction, and the extracted products are sterilized and concentrated to obtain yolk antibodies. The specific process is: on the 14th day and 28th day after the basic immunization of the laying hens with the inactivated chicken infectious bursal disease vaccine, the booster immunization was carried out respectively; the eggs were collected from 10 to 14 days after the booster immunization, and agar was used for sampling. Biphasic diffusion assay (AGP) was used to measure the titer of IBD antibody in the egg yolk of high-free eggs; after disinfection and sterilization of high-free eggs with AGP≥1:64, the yolk was separated; Extraction: filter and clarify the egg yolk extract with a 0....

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Abstract

The invention discloses an infectious bursal disease antigen-antibody complex as well as a preparation and a preparation method thereof. The preparation method of the antigen-antibody complex comprises the following steps of: (1) preparing a VP2 protein as an antigen; (2) preparing an egg yolk antibody from an infectious bursal disease virus inactivated vaccine as an antibody; and (3) mixing the antigen and the antibody according to the prescription proportion, and performing sterilization treatment. The antigen-antibody complex preparation mainly refers to a common liquid preparation, an oil emulsion and a solid preparation prepared from the infectious bursal disease antigen-antibody complex. The antigen-antibody complex disclosed by the invention has the characteristics of stable performance, good safety, fast immunization effect after animal immunization, strong immunity and high protection rate; and the prepared complex preparation is simple and quick to prepare and safe and convenient to use.

Description

technical field [0001] The invention relates to an antigen-antibody complex, a preparation and a preparation method, in particular to an antigen-antibody complex, a preparation and a preparation method for chicken infectious bursal disease. Background technique [0002] Chicken infectious bursal disease (Infectious Bursal Disease, IBD), also known as chicken infectious supracoelomal bursal disease, is an acute, contagious disease caused by infectious bursal virus. It is characterized by inflammation, necrosis, atrophy and severe damage of lymphocytes in the bursa of Fabricius, which causes immune dysfunction in chickens and interferes with the immune effects of various vaccines. Chicken infectious bursal disease has a high incidence rate, almost 100%, and a low mortality rate, generally 5% to 15%. It is currently one of the most important diseases in the poultry industry. [0003] Anti-infective drugs currently used in the prevention and treatment of chicken infectious burs...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/42A61P31/14A61K39/12
Inventor 苏建东杨保收李守军梁武
Owner TIANJIN RINGPU BIO TECH
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