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Method for preparing vaccine to chicken coccidiosis

A chicken coccidiosis and vaccine technology, which is applied in the field of chicken coccidiosis vaccine preparation, can solve the problems of uneven distribution of oocysts, disease, and unstable weakening performance, and achieves easy popularization and application, reduced use cost, and simple and practical method. Effect

Inactive Publication Date: 2007-10-03
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this kind of virulent insect seedling has not been widely used for more than 40 years. The distribution of oocysts in drinking water or feed is uneven, resulting in inconsistencies in the amount of oocysts ingested by chickens. As a result, some chickens are too lightly infected to produce immunity, and some chickens ingest too much oocysts and cause disease
However, the attenuation method of chicken embryo subculture has four disadvantages: ① the attenuation performance is relatively unstable, and the virulence returns to strong quickly when it is transferred to chicken; ② it is difficult to produce a large number of oocysts of commercial attenuated strains through chicken embryos; ③ As the number of passages of chicken embryos increases, the immunogenicity of the parasites will be reduced or lost. In fact, it is difficult to obtain the best combination that maintains good immunity and makes the virulence weaker; ④The main limitation is that the most popular Although the broad giant, pile type and Eimeria precocci can develop in chicken embryos, the growth of oocysts is poor, so the production of "full price" attenuated larvae through chicken embryo subculture is limited
Progress has been made in the understanding of the basic nature of coccidia infection and immunity by using genetic engineering technology, but it is still far away from the goal of making genetically engineered vaccines, and it will take time to transfer to the practical stage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] First carry out sporulation of oocysts, amplify chicken coccidia oocysts, put the isolated oocysts into a petri dish with 10 times the volume of 2.5% potassium dichromate solution, the solution depth is about 0.6cm, put Cultivate for 5 days in a constant temperature box at 28-30°C; during the cultivation period, gently stir the culture medium 4-5 times a day, when more than 90% of the oocysts have completed sporulation, stop cultivating and store in a 4°C refrigerator Standby; secondly, ultrasonically weaken the oocysts, add the sporulated oocysts collected above with distilled water at 3000 rpm / centrifuge twice to remove the potassium dichromate solution, put them in a 100ml small beaker, count and put the small beaker into After the 1000ml large beaker with crushed ice is ice-bathed, place it on the lifting platform of the soundproof box of the ultrasonic instrument, raise the lifting platform until the horn is immersed in the solution for 10mm, adjust the power to the...

Embodiment 2

[0042] First carry out sporulation of oocysts, amplify chicken coccidia oocysts, put the isolated oocysts into a petri dish with 10 times the volume of 2.5% potassium dichromate solution, the solution depth is about 0.6cm, put Cultivate for 5 days in a constant temperature box at a temperature of 28-30°C; during the cultivation period, gently stir the culture medium 5 times a day, when more than 90% of the oocysts have completed sporulation, stop the cultivation and store in a 4°C refrigerator for later use; Secondly, the oocysts are weakened by ultrasonic waves, and the sporulated oocysts collected above are added with distilled water at 4000 rpm / centrifugation for 3 times to remove the potassium dichromate solution, and then placed in a 100ml small beaker, and then put the small beaker into a container filled with crushed ice. After the 1000ml large beaker is ice-bathed, it is placed on the lifting platform of the soundproof box of the ultrasonic instrument, and the lifting p...

Embodiment 3

[0044] First carry out sporulation of oocysts, amplify chicken coccidia oocysts, put the isolated oocysts into a petri dish with 10 times the volume of 2.5% potassium dichromate solution, the solution depth is about 0.6cm, put Cultivate for 5 days in a constant temperature box at 28-30°C; during the cultivation period, gently stir the culture medium 4-5 times a day, when more than 90% of the oocysts have completed sporulation, stop cultivating and store in a 4°C refrigerator Standby; secondly carry out ultrasonic wave weakening oocyst, add distilled water 5000 rpm / centrifugation 3 times to the sporulated oocyst collected above and remove the potassium dichromate solution, put it in a 100ml small beaker, then put the small beaker into the After the 1000ml large beaker with crushed ice is ice-bathed, place it on the lifting platform of the soundproof box of the ultrasonic instrument, raise the lifting platform until the horn is immersed in the solution for 10mm, adjust the power ...

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Abstract

A process for preparing the vaccine of chicken coccidiosis includes such steps as separating the oogonia from chicken coccidian, culturing in the solution of potassium bichromate until more than 90% of oogonia become spores, storing in refrigerator at 4 deg.C, adding distilled water, centrifugal removal of potassium bichromate solution, counting, ice bath, and ultrasonic weakening.

Description

technical field [0001] The invention relates to a preparation method of chicken coccidiosis vaccine, which is a method for ultrasonically weakening oocysts of Eimeria tenella. Background technique [0002] Chicken coccidiosis is a widely distributed and harmful intestinal parasitic protozoan disease. It is mainly caused by the parasitic Eimeria coccidiosis in intestinal epithelial cells. It consists of nine species of Eimeria caused by coccidia. The pathogen belongs to Apicomplexa, a protozoa of the genus Eimeria in the order Eucoccus, and its life cycle is divided into three stages: schizogenesis, gametogenesis and sporulation. The first two stages are carried out in chickens, and the latter stage is carried out in vitro , during which the sporulated oocysts can be infected orally. In production, chicken coccidiosis not only causes a large number of chicks to die, but more seriously, it will significantly reduce the production performance of chickens and cause major econo...

Claims

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Application Information

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IPC IPC(8): A61K39/012A61P33/00
Inventor 赵权刘春杰
Owner JILIN AGRICULTURAL UNIV
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