162 results about "Eimeria" patented technology

Vaccines comprising TRAP polypeptides and Salmonella enteritidis vectors comprising TRAP polypeptides are provided. The vaccines may also include a CD 154 polypeptide capable of binding to CD4Q. Also provided are methods of enhancing an immune response against Apicomplexan parasites and methods of reducing morbidity associated with infection with Apicomplexan parasites.

Vaccines comprising TRAP polypeptides and Salmonella enteritidis vectors comprising TRAP polypeptides are provided. The vaccines may also include a CD154 polypeptide capable of binding to CD40. Also provided are methods of enhancing an immune response against Apicomplexan parasites and methods of reducing morbidity associated with infection with Apicomplexan parasites.

The present invention relates to a vaccine for coccidiosis in chickens prepared from three attenuated Eimeria species: E. acervulina, E. maxima and E. tenella. The vaccine was similar to or superior to other anticoccidial drugs in stimulating protective immunity against coccidiosis.

The invention discloses an Eimeria tenella AMA 1 gene, the nucleotide sequence of which includes a DNA sequence that codes the amino acid sequence represented by SEQ ID No.1 or a DNA sequence that codes the amino acid sequence which has AMA 1 protein activity and is obtained by omitting, adding, inserting or substituting one or more amino acid in the amino acid sequence represented by SEQ ID No.1. The invention also discloses proteins coded by the above-mentioned genes. The Eimeria tenella AMA 1 gene and proteins provided in the invention are closely related to invasion of daughter spores, escape and invasion of merozoites and escape and invasion of microgametes in the stage of sexual reproduction, and are suitable for being developed into novel vaccines or medicines for controlling coccidiosis.

The invention discloses an industrialized production method for separating and purifying a chicken eimeria coccidium oocyst. According to the industrialized production method, the separation and purification of the chicken eimeria coccidium oocyst are carried out by adopting the scientific assembly of a disc separator and a tubular type centrifuge, after sporulated oocysts are purified, larger impurities and extremely tiny impurities are not generated; an object plate is thoroughly subjected to microscopic examination, unsporulated oocysts are also all thoroughly removed, sporulated oocysts are all in the visual field, and the solution background is clear. The industrial production method disclosed by the invention can be used for shortening the separation and purification time of the oocysts; compared with the traditional technology, the industrial production method disclosed by the invention can be used for saving the time by more than 70%, reducing the product instability caused by excessive human factors by preventing integral oocyst purification from the disturbance of excessive artificial working and is the breakthrough of the existing coccidium vaccine production method; the industrial production method can be used for continuous production by only needing two centrifuges, thereby greatly reducing the cost input of an enterprise.

Vaccine vectors and methods of using the vaccine vectors to enhance the immune response to an Apicomplexan parasite and reduce the morbidity or morality associated with subsequent infection are provided herein. The vaccine vectors include a polynucleotide encoding a Rhomboid polypeptide and optionally include an immune-stimulatory polypeptide suitably expressed on the surface of the vaccine vector.

The invention discloses a new Eimeria tenella heat shock protein 60 (Et HSP60) gene. The cloning number is ZB2-D07, the total length is 1802 bp, and the Et HSP60 gene contains a 1455 bp complete open reading frame (ORF) and can code 484 amino acids (Genbank accession number: FJ911605). The gene is connected with a prokaryotic expression vector pET28a(+) to construct a prokaryotic expression recombinant plasmid pET28a(+)-Et HSP60 and is expressed in an escherichia coli system. SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) is carried out by purifying the recombinant protein pET28a(+)-Et HSP60, and then the recombinant protein pET28a(+)-Et HSP60 is transferred to a PVDF (Polyvinylidene Fluoride) membrane. The antiserum of immuned chicken orally taking E. tenella oocyst is taken as a primary antibody, and goat-anti-chicken IgG (immunoglobulin G) is taken as a secondary antibody to carry out Western-blot analysis. The analysis result shows that the recombinant protein pET28a(+)-Et HSP60 has certain antigenicity and can be used for preparing a medicine for resisting chicken coccidiosis and a vaccine resisting chicken coccidiosis.

The invention provides an immunoregulation type DNA vaccine with actions of preventing and treating chicken coccidiosis, which comprises sporozoite surface antigen genes TA4 of Eimeria tenella and chicken interleukin-2 genes, and a section of proteinase hydrolytic sequence is introduced to the 5' end of the chIL-2 gene.

The invention discloses an Eimeria tenella microcorticoid protein 2 related protein, and a preparation method and an application thereof. The protein is a newly discovered protein which is highly expressed by Eimeria tenella in each developmental stage. The amino acid sequence of the protein comprises an amino acid sequence represented by SEQ ID NO. 1. An EtM2AP gene is inserted into an Escherichia coli expression vector pET28a to obtain a recombinant expression vector, the recombinant expression vector is transferred into Escherichia coli to express a chicken Eimeria tenella recombinant protein (EtM2AP), and the recombinant protein is purified, and then is mixed with an adjuvant to prepare an EtM2AP recombinant protein vaccine. The vaccine can effectively increase the survival rate and the relative weight gain rate of chicks and reduce the coccidiosis oocyst discharge amount of the immunized chicks, and the EtM2AP recombinant protein vaccine has a good immune protection effect and hasthe potential of being used as a vaccine for preventing chicken Eimeria tenella coccidiosis.

The invention discloses an eimeria tenella gene. The eimeria tenella gene is an eimeria tenella elF3d gene which can code an amino acid sequence shown in SEQ ID NO.1. The invention also discloses a protein coded by the eimeria tenella eIF3d gene. Recombinant protein coded by the eimeria tenella eIF3d gene has a good immune protection effect on avian coccidiosis and is applicable to being developed into a new vaccine or new drug which can be used for preventing and treating the avian coccidiosis.

The present invention provides improved methods and compositions for producing oocysts. The oocysts produced according to the invention find use in the manufacture of vaccines. In preferred embodiments, the present invention provides methods and compositions for the production of Eimeria oocysts. Vaccines containing Eimeria oocysts, sporocysts and / or sporozoites produced according to the present invention may be used to immunize birds against coccidiosis either in ovo or post hatch.

The invention provides an eimeria tenella AN1-like Zinc finger domain-containing protein and an application thereof in inhibiting invasion of coccidium. The eimeria tenella AN1-like Zinc finger domain-containing protein is cloned, expressed and purified. The transcription level of EtAN1-ZnFP in different developmental stages of polypides is detected by fluorescence quantitative PCR (qPCR). The immune protective effect of EtAN1-ZnFP after chickens are infected with coccidium is evaluated by immunoprotection experiments. Research results indicate that the EtAN1-ZnFP is possible to participate ininvasion and growing development of eimeria tenella.