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Egg yolk antibody for preventing novel goose astrovirus and preparation method thereof

An astrovirus, egg yolk antibody technology, applied in the directions of virus antigen components, antibodies, antiviral agents, etc., can solve the problems of inconvenience, restrict the development and application of egg yolk antibody, etc., and achieve good safety, good commercialization development prospects, The effect of reducing the impurity content

Active Publication Date: 2018-09-21
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the disease is reported for the first time in my country, there is no commercial vaccine and antibody on the market, and the egg yolk antibody used for the emergency prevention and early infection treatment of the new goose astrovirus has not been reported; and because the egg yolk contains A large amount of lipids brings a lot of inconvenience to use, which also restricts the development and application of the yolk antibody for preventing and treating this novel goose astrovirus

Method used

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  • Egg yolk antibody for preventing novel goose astrovirus and preparation method thereof
  • Egg yolk antibody for preventing novel goose astrovirus and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1: Discovery and Identification of Novel Goose Astrovirus

[0060] 1. Materials and methods

[0061] 1.1 Collection and processing of disease materials:

[0062] Take liver, spleen, kidney and other tissues of dead goslings, add sterile saline at a ratio of 1:4 for homogenization, place the homogenate in the refrigerator for 3 times, centrifuge at 6000r / min for 15min, and take the supernatant The solution was filtered through a 0.22 μm filter and stored at -20°C for later use.

[0063] 1.2 Main reagents:

[0064] Goose embryos were purchased from a goose farm in Liaocheng, Shandong, the RNA extraction kit was purchased from Beijing Kangwei Century Co., Ltd., the ordinary agarose gel recovery kit was purchased from Beijing Quanshijin Company, and the reverse transcription kit was purchased from Bao Biology (Dalian ) Co., Ltd., 2×EsTaq MasterMix was purchased from Beijing Kangwei Century Co., Ltd., and DL2000Marker was purchased from Bao Biological (Dalian) Co....

Embodiment 2

[0086] Embodiment 2: the preparation of inactivated vaccine

[0087] (1) Virus propagation procedure: inoculate 9-day-old healthy SPF chicken embryos with the new goose star virus strain isolated in Example 1 at a dose of 0.2ml / piece, discard dead embryos within 24 hours, and collect dead chicken embryos within 24 to 120 hours Allantoic fluid, virus fluid obtained, stored at -20°C.

[0088] (2) Concentration of the virus liquid: Concentrate the harvested virus liquid to 1 / 3 of the original volume with an ultrafiltration concentrator at 2-8°C, and conduct a sterility test according to the appendix of the current "Chinese Veterinary Pharmacopoeia" to ensure aseptic growth , and keep samples for testing the poison price. The virus content per 0.1ml of virus liquid should be ≥10 5.0 ELD 50 , the concentrated allantoic fluid is then inactivated.

[0089] (3) Inactivation: the concentrated virus liquid is introduced into an inactivation tank, and 10% formaldehyde solution is add...

Embodiment 3

[0097] Embodiment 3: the preparation of new goose Astrovirus egg yolk antibody

[0098] 1. Use inactivated vaccines to immunize laying hens:

[0099] The new goose Astrovirus inactivated vaccine prepared in Example 2 is used to immunize laying hens, and the neck of each chicken is subcutaneously injected with 2.0ml of the inactivated vaccine for the first time, and the second immunization is carried out after 14 days, and the neck of each chicken is subcutaneously injected with 2.0ml Inactivated vaccine, the third immunization was carried out 14 days after the second immunization, and 2.0ml inactivated vaccine was injected subcutaneously in the neck of each chicken, and booster immunization was carried out 14 days after the third immunization, 2.0ml inactivated vaccine was injected subcutaneously in the neck of each chicken, 14 days after the booster immunization, the egg yolk was collected to determine the neutralizing antibody titer of the new goose Astrovirus should be ≥1:1...

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Abstract

The invention discloses an egg yolk antibody for preventing novel goose astrovirus and a preparation method thereof. The egg yolk antibody contains a goose astrovirus strain resistance antibody; the preservation number of the goose astrovirus strain is CCTCC NO:V201808. The prepared egg yolk antibody is good in safety, and no any partial or whole adverse reaction is caused by the egg yolk antibody; infections caused by novel goose astrovirus can be effectively prevented and / or treated, and the good commercialization development prospect is achieved.

Description

technical field [0001] The invention relates to the technical field of veterinary biological products, in particular to an egg yolk antibody for preventing and treating novel goose Astrovirus and a preparation method thereof. Background technique [0002] Astrovirus (AStV) is a kind of non-encapsulated, spherical, single-stranded positive-sense RNA virus with a diameter of 28-30nm. The genome is infectious and 6.4-7.9kb long. The infection of this virus has a certain prevalence. It can cause enteritis and diarrhea in humans or animals, sometimes accompanied by vomiting and abdominal pain. Astroviridae can be divided into mammalian Astrovirus genus and avian Astrovirus genus according to the different infected hosts. Avian Astrovirus can cause a variety of diseases in poultry. vary with each other. Goose astrovirus (GAstV), which mainly affects young goslings, has been prevalent in many provinces, increasing the mortality rate of geese, and greatly endangering the healthy d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/10C07K16/02A61K39/12A61P31/14
CPCA61K2039/505A61K2039/552A61P31/14C07K16/02C07K16/10C07K2317/76
Inventor 唐熠刁有祥牛晓宇
Owner SHANDONG AGRICULTURAL UNIVERSITY
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