178results about How to "Improve disease and stress resistance" patented technology

Materials and methods for gene targeting using Clustered Regularly Interspersed Short Palindromic Repeats / CRISPR-associated (CRISPR / Cas) systems are provided herein.

The invention relates to a method for producing water-soluble fertilizers, in particular to a method for producing the water-soluble fertilizers with biochemical humic acid. The method is characterized by including matrix fermentation, concentrated liquor preparation and finished product preparation: firstly, weighing prepared concentrated liquor; secondly, adding modifiers, heating and stirring the liquor in the whole process, controlling the temperature to range from 40 DEG C to 60 DEG C, and cooling the liquor to be below 30 DEG C after reaction to obtain the fertilizers; and thirdly, packaging the fertilizers. The mass ratio of the concentrated liquor, the modifiers and inorganic auxiliary materials is (55-80): (0.1-2): (20-45). The method is simple in manufacture, easy in operation and high in actionability, solves the problem of water solubility of organic fertilizers, provides nutrition, improves soil, prevents diseases and insect pests, and integrates the effects of fertilizer supplementation, bacterium resistance and disease resistance. The fertilizers have balanced nutrition, stable state and persistent fertilizer effects, crop yield can be remarkably improved, the quality of agricultural products is improved, and soil fertility is enhanced.

The invention relates to a composition for improving soil, in particular relates to a saline-alkali soil conditioner. The saline-alkali soil conditioner is prepared by mixing the following components by weight percent: 20%-35% of sulfur powder, 20%-30% of humic acid, 5%-10% of bentonite, 20%-25% of magnesium sulfate heptahydrate, 8%-12% of calcium superphosphate, 1%-2% of zinc sulfate heptahydrate and 2%-3% of boric acid. The saline-alkali soil conditioner can be used for regulating acid-base level of the soil, and effectively supplementing microelements in the soil, improving the nutritional deficiency symptom of crops, chelating harmful heavy metal elements in the soil, reducing saline and alkali degree, promoting the soil to form a granular structure, improving root growth environment of crops, enhancing saline and alkali resistance of crops, disease resistance and cultivation of resilience, reducing the harm of saline and alkali to plant roots, and releasing the effective phosphorous solidified by the soil, increasing soil fertility, holding water and saving fertilizer so as to finally and completely improve the soil, recover the soil fertility, increase both production and income and improve crop quality and utilization rate of the fertilizer.

The invention relates to a planting method of selenium-rich passiflora edulis. The variety of the planted passiflora edulis is Tainung No1. The planting method comprises steps as follows: land selection, land preparation, field planting, shelving, training and pruning, fertilizer and water management, field management and pest control. According to the planting method of the selenium-rich passiflora edulis, a selenium-rich fertilizer fermentation broth obtained through fermentation of inorganic selenium fertilizer is sprayed to leaf surfaces and fruits of the passiflora edulis, the average single fruit weight of the produced passiflora edulis is 63 g, the maximum single fruit weight is 125 g, the fruit juice is reddish yellow and has strong fragrance, the acidity is 2.14%, the highest selenium content can reach 12 mu g / kg, the sweetness reaches 23% Brix, the fruit juice rate is up to 34%, the color and luster are bright, the yield is high, pollution is prevented, and five crops are harvested every year.

The invention discloses a microbial organic fertilizer as well as a preparation method and application thereof. The preparation method of the microbial organic fertilizer comprises the following steps of: mixing five types of bacteria liquid, including Klebsiella pneumoniae subspecies, Bacillus mucilaginosus, Bacillus megatherium, Bacillus subtilis and Thermophilic sporotrichum, with an organic matter base stock in a weight ratio of 1: 1: 1: 1: 1: 500 to obtain a mixture A and fermenting the mixture A, wherein the organic matter base stock is composed of poultry excrement, straws and humic acid-like substances in a weight ratio of 3: 3: 2; externally placing one bag of Streptomycesmicroflavus bacteria powder as B; and adding the bacteria powder of B into A according to the ratio of 0.2% to obtain the microbial organic fertilizer. The microbial organic fertilizer is applied to cotton and potato planting and grape planting under an extreme drought condition and the yield increasing effect is obvious. According to the invention, the technical difficulty of the fermentation of the existing microbial organic fertilizer is effectively solved, and nitrogen fixation, phosphorous decomposition and potassium decomposition effects are comprehensively achieved; and meanwhile, the microbial organic fertilizer is convenient for farmers, is simple and practical and has important application value.

The invention relates to an efficient snail-killing type organic fertilizer produced from tea seed cakes and a preparation method thereof. The efficient snail-killing type organic fertilizer is characterized by being prepared from the following raw materials in percentage by weight: 45 to 60 percent of the tea seed cakes, 25 to 45 percent of chicken manure, 0.5 to 3 percent of phosphate fertilizer, 5 to 20 percent of organic additive, 0.5 to 3 percent of microbial decomposition agent, and 1 to 4 percent of snail-killing synergistic agent. The efficient snail-killing type organic fertilizer has the characteristics of complete nutrients, cultivatable fertility of a common organic fertilizer, can effectively kill paddy field pomacea, leeches, wireworms and the like, is biologically environment-friendly and nuisanceless, has the function of regulating the plant growth, can carry out snail-killing and fertilizing at the same time, simplifies the farming procedures, is convenient to use, saves labor power and materials, and can stimulate the growth of plant; and a contained plant growth regulator can promote the metabolism and the photosynthesis of the plants, promote the root system development, increase new roots, promote the reproductive development, and improve the disease-resistant and degeneration-resistant capacities of paddy.

The invention belongs to the field of soil conditioners and relates to an algae microbial soil conditioner specially used for saline-alkali soil. The algae microbial soil conditioner is prepared by blending the raw materials including an acidic raw material, a plant sourced organic substance, an animal sourced organic substance, a composite probiotic agent, an enzymolysed algae extract, and medium and trace elements. The soil conditioner is prepared by combining the probiotics and the algae active extract, so that on one hand, pH value of the saline-alkali soil can be adjusted, saline-alkali degree is reduced, root system growth environment of crops is improved and damage on a root system due to the salt and alkali is reduced; and on the other hand, available phosphorus, immobilized by soil, can be released, so that available microelements in soil are effectively supplied, thereby improving soil fertility. Meanwhile, alginic acid can improve the water retention and fertilizer saving capability of soil, thereby finally improving the soil completely, increasing crop quality and utilization rate of fertilizers, and achieving yield increasing.

The invention discloses a selenium-rich nutrient solution for a pear. The nutrient solution comprises, by weight, 20 to 30 parts of magnesium sulfate, 5 to 15 parts of zinc sulfate, 10 to 15 parts of copper sulfate, 10 to 25 parts of iron sulfate, 10 to 25 parts of urea, 8 to 15 parts of potassium sulfate, 10 to 15 parts of potassium dihydrogen phosphate and 25 to 40 parts of sodium selenite. Through application of the nutrient solution provided by the invention, selenium content of the pear can be substantially increased, production of the pear is promoted, net weight of fruit is increased, resistance of the pear to insect attack is enhanced, and attack by diseases and insect pests is reduced.

The invention relates to a planting method for improving the sugar content of passion fruits. The variety of the planted passion fruits is 'Tainong No.1', and the planting method comprises the steps of land selection, soil preparation, planting, frame erection, reshaping and pruning, fertilizer and water management, field management and prevention and control of diseases and insect pests. By the adoption of the planting method for improving the sugar content of the passion fruits, a fertilizer containing sugar and fermentation broth prepared through fermentation of human excrete and containing a sugar fertilizer are irrigated in a drop mode before blooming and during fruit forming, the average single-fruit weight of produced passion fruits is 63 g, the maximum weight is 125 g, fruit juice is reddish yellow and has heavy flavor, the acidity is 2.14%, the sweetness is up to 30% Brix, the fruit juice rate is up to 34%, color and luster are bright, the yield is high, no pollution exists, and five-time harvesting can be performed in one year.

The invention belongs to the field of biological organic fertilizers, and discloses a moisture-retaining and nitrogen-fixing compound microbial fertilizer. The preparation method of the fertilizer comprises the following steps: (1) preparing fermentation broth; (2) preparing compound organic matter feed liquid; (3) preparing the compound microbial fertilizer. According to the fertilizer, agricultural waste is effectively utilized, a microbial fermentation technology is used, and the soil structure is improved, and loss of nutrition in the using process is controlled, so that the fertilizer efficiency is more durable. The compound microbial fertilizer has the effects of promoting high stable yield of crops, even and strong seedlings and the like.

The invention discloses a high-efficiency microbial organic fertilizer. The high-efficiency microbial organic fertilizer is prepared from the following raw materials: filtered mud, rice bran, peanut bran, molasses, yeast waste solution, a compound fermentation strain and compound fermentation enzyme. The invention also discloses a preparation method of the high-efficiency microbial organic fertilizer. The preparation method comprises a multistage fermentation step, an ageing step and the like. According to the high-efficiency microbial organic fertilizer disclosed by the invention, the prematurity of crops can be promoted, and the yield and the quality are improved; in addition, the use amounts of fertilizer and pesticides are reduced, soil is improved and the hazards of heavy metal to the soil and the crops is eliminated.

The invention relates to a freshwater shrimp cultivation method, in particular to a pollution-free freshwater shrimp cultivation method according to which hybridization is adopted and fish larvae are matched for cultivation together. The pollution-free freshwater shrimp cultivation method includes the following steps of shrimp pond requirements, shrimp pond treatment, hybridization, shrimp larvae breeding, feeing management, water quality management and density control. The pollution-free freshwater shrimp cultivation method makes the most of idle resources for production, standardizes cultivation techniques, and obtains high-quality offspring seeds for cultivation after Lake Taihu youth shimps and local shrimps are hybridized; moreover, when a second generation of freshwater shrimps are reproduced, the fish larvae are bred, on the one hand, small shrimp larvae can be used as feed of the fish larvae, and on the other hand, space is saved by the small shrimp larvae which are eaten and oxygen consumption is reduced, and therefore unified specifications and high output of the freshwater shrimps are ensured.

The invention provides a method for preparing and using high organic matter compound microbial water-soluble fertilizer, and relates to the technical field of fertilizer. The fertilizer is prepared from the following raw materials in parts by weight: potassium fulvic acid, potassium nitrate, urea, potassium sulfate, full-water soluble ammonium phosphate, potassium hydrogen phosphate, amino acid fertilizer, humic acid, peptone, concentrated biological fermentation broth, modified biomass charcoal, microbial agent and medium trace elements. The fertilizer has the beneficial effects that organicmatters and functional microbial flora are supplied while inorganic nutrition is supplied, the effect of regulating the soil microbial flora structure is achieved, organic and inorganic matters and microbes of water-soluble fertilizer can be effectively combined, occurrence of soil obstacle of continuous cropping can be effectively relieved, occurrence of root rot diseases in a greenhouse facilityculturing process can be reduced, yield can be increased, and the quality of agricultural products can be improved.

The invention relates to a preparation and use method of a microbial agent for relieving continuous cropping obstacle of solanaceae crops, and relates to the technical field of microbial agents. The microbial agent is prepared from the following raw materials in parts by weight: bacillus methylotrophicus, bacillus subtilis, bacillus lichenniformis, bacillus colloid, saccharomycetes, photosyntheticbacteria, humic acid, potassium fulvic acid, potassium humate, amino acid fertilizer, potassium hydrogen phosphate, modified biomass carbon, alginic acid, chitin, peptone, and medium trace elements.The microbial agent has the beneficial effects that the defects of only use as base fertilizer for applying and difficulty in colonization in the common microbial agent are overcome, the occurrence ofroot rot of continuous cropping obstacle is prevented in the whole growth period of solanaceae crops, and the application is convenient; the auxiliary materials in the microbial agent are suitable for the colonization of functional microorganisms and enhancement of activity, the integrated flushing and applying effects with the water fertilizer of solanaceae crops are better, the occurrence of root rot in the continuous cropping can be better prevented, and the high output and stable output of the solanaceae crops can be promoted.

The present invention discloses a high-activity humic acid bio-fertilizer preparation method, and belongs to the technical field of agriculture. The high-activity humic acid bio-fertilizer is prepared by completely fermenting lignite, maifanite, a montan wax resin, traditional Chinese medicine residue, silkworm excrement, oil cake and compound bacteria. According to the present invention, with the high-activity humic acid bio-fertilizer, the physical and chemical properties of soil can be significantly improved, the absorption of crops on nutrients can be promoted, and the utilization rate of the chemical fertilizer can be increased; the fertilizer efficiency period is lasting and stable, the disease resistance and the stress resistance of crops are enhanced, and the yield increasing effect and the quality improving effect are significant; and the high-activity humic acid bio-fertilizer can be widely used in grain, vegetables, melon fruits and other field and cash crops, meets the development trend of green agriculture, has characteristics of wide raw material source, easy industrialization achieving, and broad application prospect.

The invention relates to a production process of lignin ammonium sulphonate as a plant root growth regulator, aiming at the problems that the traditional fertilizer has low utilization ratio, causes hardening on lands and is not beneficial to plant root growth. A formula of an organic fertilizer comprises the following materials in percentage by weight: 40%-70% of added organic raw materials, 30%-45% of straw power generation plant ash, 5%-15% of attapulgites and 2%-6% of lignin ammonium sulphonate, wherein the added organic raw materials contain more than or equal to 60% of organic matters; a formula of an inorganic compound fertilizer comprises the following materials in percentage by weight: 20%-40% of urea, 20%-30% of monoammonium phosphate, 10%-30% of potassium chloride, 2%-6% of ammonium sulfate, 2%-6% of medium trace elements, 5%-15% of attapulgites and 2%-6% of lignin ammonium sulphonate, wherein the content of the potassium chloride is 60%; and a formula of an organic-inorganic compound fertilizer comprises the following materials in percentage by weight: 10%-20% of urea, 10%-20% of monoammonium phosphate, 20%-30% of ammonium chloride, 5%-15% of potassium chloride, 2%-6% of ammonium sulfate and 10%-20% of added organic raw materials with the organic matters of more than or equal to 60%, 2%-6% of medium trace elements, 2%-6% of lignin ammonium sulphonate and 2%-6% of attapulgites. The production process comprises the following steps of: blending various raw materials in a blending disc according to the formulas; and crushing, granulating, dying, screening, cooling and packaging to form fertilizer commodities according to a general production process of producing compound fertilizers.

The invention discloses a preparation method of an organic fertilizer containing humic acid, and belongs to the technical field of agriculture. The organic fertilizer containing the humid acid is scientifically prepared from lignite, medical stone, lignite wax resin, Chinese medicine slag, wormcast, oil cake, a composite bacteria agent and common fertilizers. By the organic fertilizer containing the humic acid, physical and chemical properties of soil can be improved obviously, adsorption of crops to nutrient is promoted, and the utilization rate of chemical fertilizers is increased; the effect period of the fertilizer is lasting and stable, disease resistance and stress resistance of crops are improved, and effects of increasing yield and improving quality are obvious; and the organic fertilizer containing the humic acid can be widely used for lands for growing field crops such as cereal, vegetables, melons and fruits and the like and economic crops, and conforms to the development trend of the agriculture. In addition, sources of raw materials are extensive, industrialization is easy to implement, and the application prospect is wide.

The invention relates to the field of biostimulants and particularly relates to a preparation method for a plant growth stimulant and an application of the plant growth stimulant. The plant growth stimulant is prepared through preparing a seed solution from yellow-ocher streptomyces, inoculating the seed solution to a fermentation tank containing a mixed culture medium of maize meal, soybean meal, glutamic acid, potassium dihydrogen phosphate, sodium chloride, seaweed powder and cerealose which are in a certain ratio, carrying out fermentation, and uniformly mixing fermentation broth of the yellow-ocher streptomyces with multivitamins, multiple amino acids and algal polysaccharides which are in a certain ratio. The plant growth stimulant disclosed by the invention can be added into organic fertilizers, water-soluble fertilizers and microbicides according to a certain ratio so as to carry out synergizing, increase yield, prolong drug effect duration and enhance immunity. The plant growth stimulant disclosed by the invention has the effect of playing a role in stimulating natural courses of plants, including being beneficial to the enhancement of nutrient absorption, nutritional effects, abiotic stress resistance and crop quality, promoting the growth of roots, stems and leaves of crops, and improving the resistance of the crops, can be used for stimulating cell division of plants, promoting formation of chlorophyll and enhancing photosynthesis of the plants and has the aims of promoting growth, advancing ripeness, keeping enough flowers and young fruits, increasing yield, improving crop quality and achieving the yield increase and high yield of the crops.

The invention discloses a pesticide composition. The pesticide composition comprises an active component A and an active component B. The active component A is brassinolide or a derivative thereof, and the active component B is selected from one of strobilurin fungicides. The weight ratio of the active component A to the active component B is (0.0001-15):(1-85), and the strobilurin fungicides are selected from picoxystrobin or pyraclostrobin. The pesticide composition can be applied to the agricultural field to prevent and resist diseases or promote plant growth, and is especially applied to rice or wheat or fruit trees to adjust crop growth or improve the disease resisting effect.

The invention discloses a special fertilizer for eucalyptus and a preparation method of the special fertilizer. The fertilizer comprises the following components in part by weight: 65-85 parts of a large element basic fertilizer, 1-5 parts of medium and trace element fertilizers, 2-4 parts of humic acid, 0.1-0.5 part of microbial bacteria, 0.1-0.5 parts of a sustained release synergist, 1-4 partsof organic carbon and 10-20 parts of a filler. The special fertilizer for the eucalyptus, disclosed by the invention, has complete element compositions and can prevent an element deficiency symptom inthe growth process of the eucalyptus, thereby enhancing the resistance of the eucalyptus to an acuminate disease, reducing miscellaneous branches and enhancing the tree potential and the volume accumulation; in cooperation with the sustained release synergist, the fertilizer efficiency can be increased, and the times for applying the fertilizer by growers is reduced, so that the labor cost is reduced; finally, in match with more highly-active organic matters, carbon sources can be supplemented for the eucalyptus; while the eucalyptus photosynthesis is improved, and the volume accumulation isincreased, the carbon sources can also be taken as nutrient sources for microorganisms, so that the proliferation of the microorganisms is promoted, the soil environment is improved, and the disease and stress resistance of the eucalyptus is enhanced; and the effect on the cultivation of the eucalyptus is better.

The invention discloses a method for seedling raising and cultivation of polygonatum kingianum on the basis of seed breeding. The method comprises the following steps that 1, berries of picked polygonatum kingianum are subjected to after-ripening for 20-25 days first, and pulp parts are removed to obtain seeds of polygonatum kingianum; 2, fertilization, ploughing, pest killing and sterilization are carried out on seedling raising land; 3, sowing is carried out; 4, soil is kept moist in a seedling period, and the shading rate is controlled to be 70-80%; 5, transplanting is carried out, whereinseedlings are transplanted in a planting area with the plant spacing of 25-35 cm and the row spacing of 30-45 cm, and the seedlings are watered once 3-5 days after transplanting; 6, management in a growing period is carried out, wherein the management includes water management, weeding, topdressing, control over the shading rate and top sealing to remove moss; 7, harvesting is carried out. The method achieves refined and standardized seedling raising of polygonatum kingianum, not only is the seedling raising time shortened, the survival rate of seedlings is increased, the quality of the seedlings is improved, but also the seedling rate is high, the incidence rate of diseases and pests is low, the growth speed is high, rhizomes of polygonatum kingianum after harvesting are thick and strong,the quality is good, and the yield is high.

The invention discloses a preparation method of a liquid fertilizer for preventing and treating banana wilt.The preparation method comprises the following steps that 1, lactic acid bacteria accounting for 0.1%-0.5% of the weight of molasses-alcohol concentrated waste liquid and saccharomycetes accounting for 0.01%-0.05% of the weight of the molasses-alcohol concentrated waste liquid are added into the molasses-alcohol concentrated waste liquid, the materials are mixed to be uniform, and after natural fermentation is performed for 3 months, molasses-alcohol fermentation liquid is obtained; 2, 5-30 parts by weight of cassava extracting liquid, 2-20 parts by weight of nitrogen-phosphorus-potassium fertilizer, 0.1-5 parts by weight of medium trace elements and 5-40 parts by weight of the molasses-alcohol fermentation liquid prepared in the step 1 are put into a reactor, water is added to supplement the total parts by weight to 100, then beneficial bacteria are added, the materials are stirred to be uniform to enable the density of the beneficial bacteria to be 0.5-2 hundred million per milliliter, and the liquid fertilizer is obtained.The liquid fertilizer prepared through the method contains allelopathy inhibiting substances of fusarium oxysporum and has the positive effect on prevention and treatment of the banana wilt.

The invention belongs to the field of fertilizers, and relates to a humic acid long-acting compound fertilizer which is prepared from the following raw materials in parts by weight: 20-25 parts of humic acid, 2-4 parts of microbial inoculum, 4-8 parts of zeolite powder, 5-10 parts of magnesium powder, 5-10 parts of calcium powder, 15-20 parts of urea, 10-15 parts of monoammonium phosphate and 10-15 parts of potassium chloride. The invention also discloses a preparation method of the humic acid long-acting compound fertilizer. The humic acid long-acting compound fertilizer provided by the invention can effectively control the loss of fertilizer and moisture, enhance the yield of the crops, and greatly lower the cost.

The invention provides a wood frog stereoscopic cultivation technique. The wood frog stereoscopic cultivation technique relates to a wood frog cultivation ecological system, a wood frog anti-escape method, a wood frog cultivation harm prevention method and a feeding method for increasing the wood frog survival rate and the oil yield. The facilities used in the wood frog cultivation ecological system comprise a frog house and a grape trellis. A wall used in the wood frog anti-escape method is built by stones or bricks, the wall is of a T shape, and the top of the wall is covered by an inverted U-shaped alloy aluminum plate. The wood frog cultivation harm prevention method comprises the method for preventing harm caused by snakes, mice and cats and the method for preventing harm caused by birds. The feeding method for increasing the wood frog survival rate and the oil yield is achieved through the following steps that (1) after being hatched, firstly, tadpoles eat egg-covered egg membranes for 7-10 days, and then the tadpoles eat bait after eating up the egg membranes; (2) after the tails of froglets are completely absorbed, small yellow mealworms which are 0.6-0.8mm in length begin to be utilized to feed the froglets; (3) after 10-15 days, yellow mealworms which are 1-1.2mm in length are utilized to feed the froglets; (4) after three months, the yellow mealworms which are 1.5-2mm in length are utilized to feed the froglets.

The invention belongs to the technical field of fermentation and discloses a new technology for extracting L-glutamine. The new technology for extracting the L-glutamine comprises the following steps: fermenting, carrying out micromembrane filtration, concentrating, crystallizing, carrying out ion exchange, recrystalizing, and preparing a fertilizer. The new technology for extracting the L-glutamine has the advantages that fermentation efficiency is high, an extraction technology is easy and feasible, environmental protection and no pollution are realized, and waste materials are changed into things of value.

The invention provides a synergistic kasugamycin composition containing tea saponin. The kasugamycin composition has insecticidal, bactericidal and antiviral functions and is capable of inducing a plant to generate a disease-resistant function; the composition specifically comprises 1 to 50% of kasugamycin, 9 to 70% of tea saponin, 1 to 10% of salicylic acid and 5 to 30% of a surfactant, with the balance being a carrier or accessory material. The composition provided by the invention has stable properties; a formula for the composition is reasonable; a wettable powder, a water dispersible granule, a water aqua, a soluble powder and a soluble granule containing 1 to 50% of the effective component kasugamycin can be prepared by using a production method for a common pesticide preparation; the composition has wide-spectrum prevention and treatment effects on bacteria, fungi, viruses and insects on crops, can induce a plant to generate stress resistance and is a purely biological pesticide composition.

The invention belongs to the technical field of fertilizers and discloses a bio-organic fertilizer with a soil remediation function. The bio-organic fertilizer is prepared from the following raw materials in parts by weight: 10-12 parts of an ingredient A, 4-6 parts of an ingredient B, 3-5 parts of an ingredient C and 1-2 parts of a microbial preparation. The bio-organic fertilizer disclosed by the invention has a relatively good fertilizer effect, can be used for removing pesticide residues from soil and can be used for remedying contaminated soil.

The invention discloses a plant growth regulator. The plant growth regulator is prepared from an effective ingredient A and an effective ingredient B, wherein the effective ingredient A is thidiazuron, and the effective ingredient B is amino-oligosaccharin. The invention also provides a preparation method and application of the composition. A test result proves that the composition disclosed by the invention can regulate growth of crops preferably, improve disease resistance and stress resistance, promote a health effect of the crops, and improve the yield and the quality of the crops.

The invention discloses a carbon-based bio-organic fertilizer for improving degraded vegetable soil and application thereof. The carbon-based bio-organic fertilizer comprises, by mass percentage, 30-40% of straw biochar, 5-15% of potassium fulvate, 5-10% of amino acid powder, and the balance an organic carrier rich in BS211 and L3 bacillus amyloliquefaciens. The carbon-based bio-organic fertilizerprovided by the invention has an obvious improvement effect on degraded vegetable soil, can effectively replace fertilizers, increase the soil organic matter content, improve the soil microbial community structure, reduce vegetable soil-borne diseases and promote crop growth, and is beneficial to yield and income increase.